Source 1primary paper2022
Toolkit/CRY-BARs
CRY-BARs
Also known as: CRY-BAR, light-gated I-BAR domain containing tools
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
CRY-BARs are a family of light-gated optogenetic tools that contain an I-BAR domain and are designed to remodel membrane architecture. They are intended to provide spatial and temporal control over cellular processes involving membrane protrusion formation.
Usefulness & Problems
Why this is useful
CRY-BARs are useful for experimentally controlling and monitoring membrane architecture remodeling and associated cellular dynamics with light. Source evidence indicates application in cell lines and primary neuron cultures for reporting membrane dynamic changes associated with cellular activity.
Source:
with applications in the remodeling of membrane architectures and the control of cellular dynamics
Source:
Using cell lines and primary neuron cultures, we demonstrate that the CRY-BAR optogenetic tool reports membrane dynamic changes associated with cellular activity.
Problem solved
CRY-BARs address the need for a light-responsive system to manipulate membrane protrusions and membrane architecture in living cells. The available evidence supports their use for controlling cellular dynamics linked to membrane remodeling, but does not provide more specific benchmarking against prior tools.
Source:
with applications in the remodeling of membrane architectures and the control of cellular dynamics
Source:
Using cell lines and primary neuron cultures, we demonstrate that the CRY-BAR optogenetic tool reports membrane dynamic changes associated with cellular activity.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Techniques
Computational DesignTarget processes
No target processes tagged yet.
Input: Light
Implementation Constraints
The tool is described as a multi-component, light-gated construct family containing an I-BAR domain, consistent with implementation by domain fusion. The supplied evidence does not specify the exact construct architecture, chromophore requirements, expression strategy, or illumination parameters.
The supplied evidence does not report quantitative performance metrics, activation wavelengths, kinetics, reversibility, or comparative performance. Independent replication is not provided in the evidence, and validation appears limited to the originating study.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
CRY-BARs have applications in remodeling membrane architectures and controlling cellular dynamics.
with applications in the remodeling of membrane architectures and the control of cellular dynamics
CRY-BARs have applications in remodeling membrane architectures and controlling cellular dynamics.
with applications in the remodeling of membrane architectures and the control of cellular dynamics
CRY-BARs have applications in remodeling membrane architectures and controlling cellular dynamics.
with applications in the remodeling of membrane architectures and the control of cellular dynamics
CRY-BARs have applications in remodeling membrane architectures and controlling cellular dynamics.
with applications in the remodeling of membrane architectures and the control of cellular dynamics
CRY-BARs have applications in remodeling membrane architectures and controlling cellular dynamics.
with applications in the remodeling of membrane architectures and the control of cellular dynamics
CRY-BARs have applications in remodeling membrane architectures and controlling cellular dynamics.
with applications in the remodeling of membrane architectures and the control of cellular dynamics
CRY-BARs have applications in remodeling membrane architectures and controlling cellular dynamics.
with applications in the remodeling of membrane architectures and the control of cellular dynamics
In cell lines and primary neuron cultures, the CRY-BAR optogenetic tool reports membrane dynamic changes associated with cellular activity.
Using cell lines and primary neuron cultures, we demonstrate that the CRY-BAR optogenetic tool reports membrane dynamic changes associated with cellular activity.
In cell lines and primary neuron cultures, the CRY-BAR optogenetic tool reports membrane dynamic changes associated with cellular activity.
Using cell lines and primary neuron cultures, we demonstrate that the CRY-BAR optogenetic tool reports membrane dynamic changes associated with cellular activity.
In cell lines and primary neuron cultures, the CRY-BAR optogenetic tool reports membrane dynamic changes associated with cellular activity.
Using cell lines and primary neuron cultures, we demonstrate that the CRY-BAR optogenetic tool reports membrane dynamic changes associated with cellular activity.
In cell lines and primary neuron cultures, the CRY-BAR optogenetic tool reports membrane dynamic changes associated with cellular activity.
Using cell lines and primary neuron cultures, we demonstrate that the CRY-BAR optogenetic tool reports membrane dynamic changes associated with cellular activity.
In cell lines and primary neuron cultures, the CRY-BAR optogenetic tool reports membrane dynamic changes associated with cellular activity.
Using cell lines and primary neuron cultures, we demonstrate that the CRY-BAR optogenetic tool reports membrane dynamic changes associated with cellular activity.
In cell lines and primary neuron cultures, the CRY-BAR optogenetic tool reports membrane dynamic changes associated with cellular activity.
Using cell lines and primary neuron cultures, we demonstrate that the CRY-BAR optogenetic tool reports membrane dynamic changes associated with cellular activity.
In cell lines and primary neuron cultures, the CRY-BAR optogenetic tool reports membrane dynamic changes associated with cellular activity.
Using cell lines and primary neuron cultures, we demonstrate that the CRY-BAR optogenetic tool reports membrane dynamic changes associated with cellular activity.
The paper describes the design and implementation of a family of versatile light-gated I-BAR-domain-containing tools called CRY-BARs.
In this work, we describe the design and implementation of a family of versatile light-gated I-BAR domain containing tools (‘CRY-BARs’)
The paper describes the design and implementation of a family of versatile light-gated I-BAR-domain-containing tools called CRY-BARs.
In this work, we describe the design and implementation of a family of versatile light-gated I-BAR domain containing tools (‘CRY-BARs’)
The paper describes the design and implementation of a family of versatile light-gated I-BAR-domain-containing tools called CRY-BARs.
In this work, we describe the design and implementation of a family of versatile light-gated I-BAR domain containing tools (‘CRY-BARs’)
The paper describes the design and implementation of a family of versatile light-gated I-BAR-domain-containing tools called CRY-BARs.
In this work, we describe the design and implementation of a family of versatile light-gated I-BAR domain containing tools (‘CRY-BARs’)
The paper describes the design and implementation of a family of versatile light-gated I-BAR-domain-containing tools called CRY-BARs.
In this work, we describe the design and implementation of a family of versatile light-gated I-BAR domain containing tools (‘CRY-BARs’)
The paper describes the design and implementation of a family of versatile light-gated I-BAR-domain-containing tools called CRY-BARs.
In this work, we describe the design and implementation of a family of versatile light-gated I-BAR domain containing tools (‘CRY-BARs’)
The paper describes the design and implementation of a family of versatile light-gated I-BAR-domain-containing tools called CRY-BARs.
In this work, we describe the design and implementation of a family of versatile light-gated I-BAR domain containing tools (‘CRY-BARs’)
CRY-BARs can be used for spatial and temporal control of cellular processes that require induction of membrane protrusions by leveraging the intrinsic membrane-binding propensity of the I-BAR domain.
By taking advantage of the intrinsic membrane binding propensity of the I-BAR domain, CRY-BARs can be used for spatial and temporal control of cellular processes that require induction of membrane protrusions.
CRY-BARs can be used for spatial and temporal control of cellular processes that require induction of membrane protrusions by leveraging the intrinsic membrane-binding propensity of the I-BAR domain.
By taking advantage of the intrinsic membrane binding propensity of the I-BAR domain, CRY-BARs can be used for spatial and temporal control of cellular processes that require induction of membrane protrusions.
CRY-BARs can be used for spatial and temporal control of cellular processes that require induction of membrane protrusions by leveraging the intrinsic membrane-binding propensity of the I-BAR domain.
By taking advantage of the intrinsic membrane binding propensity of the I-BAR domain, CRY-BARs can be used for spatial and temporal control of cellular processes that require induction of membrane protrusions.
CRY-BARs can be used for spatial and temporal control of cellular processes that require induction of membrane protrusions by leveraging the intrinsic membrane-binding propensity of the I-BAR domain.
By taking advantage of the intrinsic membrane binding propensity of the I-BAR domain, CRY-BARs can be used for spatial and temporal control of cellular processes that require induction of membrane protrusions.
CRY-BARs can be used for spatial and temporal control of cellular processes that require induction of membrane protrusions by leveraging the intrinsic membrane-binding propensity of the I-BAR domain.
By taking advantage of the intrinsic membrane binding propensity of the I-BAR domain, CRY-BARs can be used for spatial and temporal control of cellular processes that require induction of membrane protrusions.
CRY-BARs can be used for spatial and temporal control of cellular processes that require induction of membrane protrusions by leveraging the intrinsic membrane-binding propensity of the I-BAR domain.
By taking advantage of the intrinsic membrane binding propensity of the I-BAR domain, CRY-BARs can be used for spatial and temporal control of cellular processes that require induction of membrane protrusions.
CRY-BARs can be used for spatial and temporal control of cellular processes that require induction of membrane protrusions by leveraging the intrinsic membrane-binding propensity of the I-BAR domain.
By taking advantage of the intrinsic membrane binding propensity of the I-BAR domain, CRY-BARs can be used for spatial and temporal control of cellular processes that require induction of membrane protrusions.
Ezrin acts as a relay between the plasma membrane and the actin cytoskeleton and is an important mediator of CRY-BAR switch function.
Moreover, we provide evidence that Ezrin acts as a relay between the plasma membrane and the actin cytoskeleton and therefore is an important mediator of switch function.
Ezrin acts as a relay between the plasma membrane and the actin cytoskeleton and is an important mediator of CRY-BAR switch function.
Moreover, we provide evidence that Ezrin acts as a relay between the plasma membrane and the actin cytoskeleton and therefore is an important mediator of switch function.
Ezrin acts as a relay between the plasma membrane and the actin cytoskeleton and is an important mediator of CRY-BAR switch function.
Moreover, we provide evidence that Ezrin acts as a relay between the plasma membrane and the actin cytoskeleton and therefore is an important mediator of switch function.
Ezrin acts as a relay between the plasma membrane and the actin cytoskeleton and is an important mediator of CRY-BAR switch function.
Moreover, we provide evidence that Ezrin acts as a relay between the plasma membrane and the actin cytoskeleton and therefore is an important mediator of switch function.
Ezrin acts as a relay between the plasma membrane and the actin cytoskeleton and is an important mediator of CRY-BAR switch function.
Moreover, we provide evidence that Ezrin acts as a relay between the plasma membrane and the actin cytoskeleton and therefore is an important mediator of switch function.
Ezrin acts as a relay between the plasma membrane and the actin cytoskeleton and is an important mediator of CRY-BAR switch function.
Moreover, we provide evidence that Ezrin acts as a relay between the plasma membrane and the actin cytoskeleton and therefore is an important mediator of switch function.
Ezrin acts as a relay between the plasma membrane and the actin cytoskeleton and is an important mediator of CRY-BAR switch function.
Moreover, we provide evidence that Ezrin acts as a relay between the plasma membrane and the actin cytoskeleton and therefore is an important mediator of switch function.
CRY-BARs hold promise as a useful addition to the optogenetic toolkit for studying membrane remodeling in live cells.
Overall, CRY-BARs hold promise as a useful addition to the optogenetic toolkit to study membrane remodeling in live cells.
CRY-BARs hold promise as a useful addition to the optogenetic toolkit for studying membrane remodeling in live cells.
Overall, CRY-BARs hold promise as a useful addition to the optogenetic toolkit to study membrane remodeling in live cells.
CRY-BARs hold promise as a useful addition to the optogenetic toolkit for studying membrane remodeling in live cells.
Overall, CRY-BARs hold promise as a useful addition to the optogenetic toolkit to study membrane remodeling in live cells.
CRY-BARs hold promise as a useful addition to the optogenetic toolkit for studying membrane remodeling in live cells.
Overall, CRY-BARs hold promise as a useful addition to the optogenetic toolkit to study membrane remodeling in live cells.
CRY-BARs hold promise as a useful addition to the optogenetic toolkit for studying membrane remodeling in live cells.
Overall, CRY-BARs hold promise as a useful addition to the optogenetic toolkit to study membrane remodeling in live cells.
CRY-BARs hold promise as a useful addition to the optogenetic toolkit for studying membrane remodeling in live cells.
Overall, CRY-BARs hold promise as a useful addition to the optogenetic toolkit to study membrane remodeling in live cells.
CRY-BARs hold promise as a useful addition to the optogenetic toolkit for studying membrane remodeling in live cells.
Overall, CRY-BARs hold promise as a useful addition to the optogenetic toolkit to study membrane remodeling in live cells.
Approval Evidence
1 source6 linked approval claimsfirst-pass slug cry-bars
we describe the design and implementation of a family of versatile light-gated I-BAR domain containing tools (‘CRY-BARs’)
Source:
applicationsupports
CRY-BARs have applications in remodeling membrane architectures and controlling cellular dynamics.
with applications in the remodeling of membrane architectures and the control of cellular dynamics
Source:
application demo resultsupports
In cell lines and primary neuron cultures, the CRY-BAR optogenetic tool reports membrane dynamic changes associated with cellular activity.
Using cell lines and primary neuron cultures, we demonstrate that the CRY-BAR optogenetic tool reports membrane dynamic changes associated with cellular activity.
Source:
design and implementationsupports
The paper describes the design and implementation of a family of versatile light-gated I-BAR-domain-containing tools called CRY-BARs.
In this work, we describe the design and implementation of a family of versatile light-gated I-BAR domain containing tools (‘CRY-BARs’)
Source:
mechanism or functionsupports
CRY-BARs can be used for spatial and temporal control of cellular processes that require induction of membrane protrusions by leveraging the intrinsic membrane-binding propensity of the I-BAR domain.
By taking advantage of the intrinsic membrane binding propensity of the I-BAR domain, CRY-BARs can be used for spatial and temporal control of cellular processes that require induction of membrane protrusions.
Source:
mechanistic rolesupports
Ezrin acts as a relay between the plasma membrane and the actin cytoskeleton and is an important mediator of CRY-BAR switch function.
Moreover, we provide evidence that Ezrin acts as a relay between the plasma membrane and the actin cytoskeleton and therefore is an important mediator of switch function.
Source:
toolkit positioningsupports
CRY-BARs hold promise as a useful addition to the optogenetic toolkit for studying membrane remodeling in live cells.
Overall, CRY-BARs hold promise as a useful addition to the optogenetic toolkit to study membrane remodeling in live cells.
Source:
Comparisons
Source-backed strengths
A key strength is that CRY-BARs were presented as a family of versatile light-gated I-BAR-domain-containing tools for membrane architecture remodeling. They were reported in both cell lines and primary neuron cultures, indicating use beyond a single cellular context.
Ranked Citations
- 1.