Source 1primary paper2017Nucleic Acids Research
Toolkit/CRY2 and LOV-fused degron light-responsive repression/degradation system
CRY2 and LOV-fused degron light-responsive repression/degradation system
Also known as: CRY2 and a LOV-fused degron system
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The CRY2 and LOV-fused degron light-responsive repression/degradation system is a multi-component optogenetic platform reported in mammalian cells that uses light to simultaneously block transcription and deplete protein levels. It is based on Arabidopsis cryptochrome 2 (CRY2), which interacts with CIB1 upon illumination, together with a LOV-fused degron configuration.
Usefulness & Problems
Why this is useful
This system is useful because it couples light control of transcriptional repression with light-driven protein depletion in a single framework, enabling bidirectional regulation of gene expression. The cited work states that such tools enable precise bidirectional control of gene expression across a variety of cells and model systems.
Source:
These tools will allow precise, bi-directional control of gene expression in a variety of cells and model systems.
Source:
a system using CRY2 and a LOV-fused degron to simultaneously block transcription and deplete protein levels with light
Source:
a system using CRY2 and CIB1 to induce protein expression with light through stimulation of transcription
Problem solved
This tool addresses the problem of coordinating rapid, light-triggered suppression of gene output at more than one regulatory layer. Specifically, the evidence supports a design intended to simultaneously block transcription and deplete protein levels with light.
Problem links
Need conditional protein clearance
DerivedThe CRY2 and LOV-fused degron light-responsive repression/degradation system is a multi-component optogenetic platform that uses light to simultaneously block transcription and deplete protein levels. It is based on Arabidopsis cryptochrome 2 (CRY2) and a LOV-fused degron configuration reported in mammalian cells.
Need precise spatiotemporal control with light input
DerivedThe CRY2 and LOV-fused degron light-responsive repression/degradation system is a multi-component optogenetic platform that uses light to simultaneously block transcription and deplete protein levels. It is based on Arabidopsis cryptochrome 2 (CRY2) and a LOV-fused degron configuration reported in mammalian cells.
Need tighter control over gene expression timing or amplitude
DerivedThe CRY2 and LOV-fused degron light-responsive repression/degradation system is a multi-component optogenetic platform that uses light to simultaneously block transcription and deplete protein levels. It is based on Arabidopsis cryptochrome 2 (CRY2) and a LOV-fused degron configuration reported in mammalian cells.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
Degradationlight-dependent protein degradationlight-dependent protein degradationlight-dependent subcellular redistributionlight-dependent subcellular redistributionlight-induced heterodimerizationlight-induced heterodimerizationTechniques
No technique tags yet.
Target processes
degradationtranscriptionInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: multi component delivery burdenimplementation constraint: spectral hardware requirementoperating role: regulatorswitch architecture: multi componentswitch architecture: recruitment
The system is multi-component and relies on CRY2 together with CIB1, since CRY2-CIB1 interaction upon illumination is explicitly supported by the source. The nuclear clearing phenotype depended on a dimerization domain in CRY2-fused transcriptional activators, indicating that construct architecture and domain fusion are important design variables; the evidence otherwise gives limited practical detail on expression, delivery, or cofactor requirements.
The supplied evidence does not provide quantitative performance metrics such as repression magnitude, degradation kinetics, reversibility, or wavelength dependence for the combined CRY2/LOV-degron configuration. Independent replication beyond the cited 2017 study is not provided, and validation details for organisms or cell types using the exact combined system are limited.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
These tools enable precise bidirectional control of gene expression in a variety of cells and model systems.
These tools will allow precise, bi-directional control of gene expression in a variety of cells and model systems.
These tools enable precise bidirectional control of gene expression in a variety of cells and model systems.
These tools will allow precise, bi-directional control of gene expression in a variety of cells and model systems.
These tools enable precise bidirectional control of gene expression in a variety of cells and model systems.
These tools will allow precise, bi-directional control of gene expression in a variety of cells and model systems.
These tools enable precise bidirectional control of gene expression in a variety of cells and model systems.
These tools will allow precise, bi-directional control of gene expression in a variety of cells and model systems.
These tools enable precise bidirectional control of gene expression in a variety of cells and model systems.
These tools will allow precise, bi-directional control of gene expression in a variety of cells and model systems.
These tools enable precise bidirectional control of gene expression in a variety of cells and model systems.
These tools will allow precise, bi-directional control of gene expression in a variety of cells and model systems.
These tools enable precise bidirectional control of gene expression in a variety of cells and model systems.
These tools will allow precise, bi-directional control of gene expression in a variety of cells and model systems.
These tools enable precise bidirectional control of gene expression in a variety of cells and model systems.
These tools will allow precise, bi-directional control of gene expression in a variety of cells and model systems.
These tools enable precise bidirectional control of gene expression in a variety of cells and model systems.
These tools will allow precise, bi-directional control of gene expression in a variety of cells and model systems.
These tools enable precise bidirectional control of gene expression in a variety of cells and model systems.
These tools will allow precise, bi-directional control of gene expression in a variety of cells and model systems.
These tools enable precise bidirectional control of gene expression in a variety of cells and model systems.
These tools will allow precise, bi-directional control of gene expression in a variety of cells and model systems.
These tools enable precise bidirectional control of gene expression in a variety of cells and model systems.
These tools will allow precise, bi-directional control of gene expression in a variety of cells and model systems.
These tools enable precise bidirectional control of gene expression in a variety of cells and model systems.
These tools will allow precise, bi-directional control of gene expression in a variety of cells and model systems.
These tools enable precise bidirectional control of gene expression in a variety of cells and model systems.
These tools will allow precise, bi-directional control of gene expression in a variety of cells and model systems.
These tools enable precise bidirectional control of gene expression in a variety of cells and model systems.
These tools will allow precise, bi-directional control of gene expression in a variety of cells and model systems.
These tools enable precise bidirectional control of gene expression in a variety of cells and model systems.
These tools will allow precise, bi-directional control of gene expression in a variety of cells and model systems.
These tools enable precise bidirectional control of gene expression in a variety of cells and model systems.
These tools will allow precise, bi-directional control of gene expression in a variety of cells and model systems.
CRY2 and CIB1 interact upon light illumination.
CRY2 and CIB1, Arabidopsis proteins that interact upon light illumination
CRY2 and CIB1 interact upon light illumination.
CRY2 and CIB1, Arabidopsis proteins that interact upon light illumination
CRY2 and CIB1 interact upon light illumination.
CRY2 and CIB1, Arabidopsis proteins that interact upon light illumination
CRY2 and CIB1 interact upon light illumination.
CRY2 and CIB1, Arabidopsis proteins that interact upon light illumination
CRY2 and CIB1 interact upon light illumination.
CRY2 and CIB1, Arabidopsis proteins that interact upon light illumination
The nuclear clearing phenotype depended on the presence of a dimerization domain in CRY2-fused transcriptional activators.
The nuclear clearing phenotype was dependent on the presence of a dimerization domain contained within the CRY2-fused transcriptional activators.
The nuclear clearing phenotype depended on the presence of a dimerization domain in CRY2-fused transcriptional activators.
The nuclear clearing phenotype was dependent on the presence of a dimerization domain contained within the CRY2-fused transcriptional activators.
The nuclear clearing phenotype depended on the presence of a dimerization domain in CRY2-fused transcriptional activators.
The nuclear clearing phenotype was dependent on the presence of a dimerization domain contained within the CRY2-fused transcriptional activators.
The nuclear clearing phenotype depended on the presence of a dimerization domain in CRY2-fused transcriptional activators.
The nuclear clearing phenotype was dependent on the presence of a dimerization domain contained within the CRY2-fused transcriptional activators.
The nuclear clearing phenotype depended on the presence of a dimerization domain in CRY2-fused transcriptional activators.
The nuclear clearing phenotype was dependent on the presence of a dimerization domain contained within the CRY2-fused transcriptional activators.
In mammalian cells, CRY2-tethered proteins showed light-dependent redistribution and clearing within the nucleus.
While adopting this approach to regulate transcription in mammalian cells, we observed light-dependent redistribution and clearing of CRY2-tethered proteins within the nucleus.
In mammalian cells, CRY2-tethered proteins showed light-dependent redistribution and clearing within the nucleus.
While adopting this approach to regulate transcription in mammalian cells, we observed light-dependent redistribution and clearing of CRY2-tethered proteins within the nucleus.
In mammalian cells, CRY2-tethered proteins showed light-dependent redistribution and clearing within the nucleus.
While adopting this approach to regulate transcription in mammalian cells, we observed light-dependent redistribution and clearing of CRY2-tethered proteins within the nucleus.
In mammalian cells, CRY2-tethered proteins showed light-dependent redistribution and clearing within the nucleus.
While adopting this approach to regulate transcription in mammalian cells, we observed light-dependent redistribution and clearing of CRY2-tethered proteins within the nucleus.
In mammalian cells, CRY2-tethered proteins showed light-dependent redistribution and clearing within the nucleus.
While adopting this approach to regulate transcription in mammalian cells, we observed light-dependent redistribution and clearing of CRY2-tethered proteins within the nucleus.
A CRY2-CIB1 system was developed to induce protein expression with light through stimulation of transcription.
a system using CRY2 and CIB1 to induce protein expression with light through stimulation of transcription
A CRY2-CIB1 system was developed to induce protein expression with light through stimulation of transcription.
a system using CRY2 and CIB1 to induce protein expression with light through stimulation of transcription
A CRY2-CIB1 system was developed to induce protein expression with light through stimulation of transcription.
a system using CRY2 and CIB1 to induce protein expression with light through stimulation of transcription
A CRY2-CIB1 system was developed to induce protein expression with light through stimulation of transcription.
a system using CRY2 and CIB1 to induce protein expression with light through stimulation of transcription
A CRY2-CIB1 system was developed to induce protein expression with light through stimulation of transcription.
a system using CRY2 and CIB1 to induce protein expression with light through stimulation of transcription
A CRY2-CIB1 system was developed to induce protein expression with light through stimulation of transcription.
a system using CRY2 and CIB1 to induce protein expression with light through stimulation of transcription
A CRY2-CIB1 system was developed to induce protein expression with light through stimulation of transcription.
a system using CRY2 and CIB1 to induce protein expression with light through stimulation of transcription
A CRY2-CIB1 system was developed to induce protein expression with light through stimulation of transcription.
a system using CRY2 and CIB1 to induce protein expression with light through stimulation of transcription
A CRY2-CIB1 system was developed to induce protein expression with light through stimulation of transcription.
a system using CRY2 and CIB1 to induce protein expression with light through stimulation of transcription
A CRY2-CIB1 system was developed to induce protein expression with light through stimulation of transcription.
a system using CRY2 and CIB1 to induce protein expression with light through stimulation of transcription
A CRY2 plus LOV-fused degron system was developed to simultaneously block transcription and deplete protein levels with light.
a system using CRY2 and a LOV-fused degron to simultaneously block transcription and deplete protein levels with light
A CRY2 plus LOV-fused degron system was developed to simultaneously block transcription and deplete protein levels with light.
a system using CRY2 and a LOV-fused degron to simultaneously block transcription and deplete protein levels with light
A CRY2 plus LOV-fused degron system was developed to simultaneously block transcription and deplete protein levels with light.
a system using CRY2 and a LOV-fused degron to simultaneously block transcription and deplete protein levels with light
A CRY2 plus LOV-fused degron system was developed to simultaneously block transcription and deplete protein levels with light.
a system using CRY2 and a LOV-fused degron to simultaneously block transcription and deplete protein levels with light
A CRY2 plus LOV-fused degron system was developed to simultaneously block transcription and deplete protein levels with light.
a system using CRY2 and a LOV-fused degron to simultaneously block transcription and deplete protein levels with light
A CRY2 plus LOV-fused degron system was developed to simultaneously block transcription and deplete protein levels with light.
a system using CRY2 and a LOV-fused degron to simultaneously block transcription and deplete protein levels with light
A CRY2 plus LOV-fused degron system was developed to simultaneously block transcription and deplete protein levels with light.
a system using CRY2 and a LOV-fused degron to simultaneously block transcription and deplete protein levels with light
A CRY2 plus LOV-fused degron system was developed to simultaneously block transcription and deplete protein levels with light.
a system using CRY2 and a LOV-fused degron to simultaneously block transcription and deplete protein levels with light
A CRY2 plus LOV-fused degron system was developed to simultaneously block transcription and deplete protein levels with light.
a system using CRY2 and a LOV-fused degron to simultaneously block transcription and deplete protein levels with light
A CRY2 plus LOV-fused degron system was developed to simultaneously block transcription and deplete protein levels with light.
a system using CRY2 and a LOV-fused degron to simultaneously block transcription and deplete protein levels with light
A CRY2 plus LOV-fused degron system was developed to simultaneously block transcription and deplete protein levels with light.
a system using CRY2 and a LOV-fused degron to simultaneously block transcription and deplete protein levels with light
A CRY2 plus LOV-fused degron system was developed to simultaneously block transcription and deplete protein levels with light.
a system using CRY2 and a LOV-fused degron to simultaneously block transcription and deplete protein levels with light
A CRY2 plus LOV-fused degron system was developed to simultaneously block transcription and deplete protein levels with light.
a system using CRY2 and a LOV-fused degron to simultaneously block transcription and deplete protein levels with light
A CRY2 plus LOV-fused degron system was developed to simultaneously block transcription and deplete protein levels with light.
a system using CRY2 and a LOV-fused degron to simultaneously block transcription and deplete protein levels with light
A CRY2 plus LOV-fused degron system was developed to simultaneously block transcription and deplete protein levels with light.
a system using CRY2 and a LOV-fused degron to simultaneously block transcription and deplete protein levels with light
A CRY2 plus LOV-fused degron system was developed to simultaneously block transcription and deplete protein levels with light.
a system using CRY2 and a LOV-fused degron to simultaneously block transcription and deplete protein levels with light
A CRY2 plus LOV-fused degron system was developed to simultaneously block transcription and deplete protein levels with light.
a system using CRY2 and a LOV-fused degron to simultaneously block transcription and deplete protein levels with light
Approval Evidence
1 source2 linked approval claimsfirst-pass slug cry2-and-lov-fused-degron-light-responsive-repression-degradation-system
a system using CRY2 and a LOV-fused degron to simultaneously block transcription and deplete protein levels with light
Source:
capability statementsupports
These tools enable precise bidirectional control of gene expression in a variety of cells and model systems.
These tools will allow precise, bi-directional control of gene expression in a variety of cells and model systems.
Source:
tool functionsupports
A CRY2 plus LOV-fused degron system was developed to simultaneously block transcription and deplete protein levels with light.
a system using CRY2 and a LOV-fused degron to simultaneously block transcription and deplete protein levels with light
Source:
Comparisons
Source-backed strengths
Evidence supports light-induced CRY2-CIB1 interaction and light-dependent redistribution/clearing of CRY2-tethered proteins within the nucleus in mammalian cells. The source also reports that these optogenetic tools provide precise bidirectional control of gene expression in multiple cellular and model-system contexts.
Compared with Cry2
CRY2 and LOV-fused degron light-responsive repression/degradation system and Cry2 address a similar problem space because they share degradation, transcription.
Shared frame: same top-level item type; shared target processes: degradation, transcription; shared mechanisms: degradation; same primary input modality: light
Strengths here: may avoid an exogenous cofactor requirement.
Relative tradeoffs: appears more independently replicated.
Compared with CRY2-CIB1 light-inducible transcription system
CRY2 and LOV-fused degron light-responsive repression/degradation system and CRY2-CIB1 light-inducible transcription system address a similar problem space because they share transcription.
Shared frame: same top-level item type; shared target processes: transcription; shared mechanisms: light-dependent subcellular redistribution, light-induced heterodimerization; same primary input modality: light
Compared with mOptoT7
CRY2 and LOV-fused degron light-responsive repression/degradation system and mOptoT7 address a similar problem space because they share transcription.
Shared frame: same top-level item type; shared target processes: transcription; shared mechanisms: light-induced heterodimerization; same primary input modality: light
Relative tradeoffs: appears more independently replicated; looks easier to implement in practice.
Ranked Citations
- 1.