Source 1primary paper2017Nucleic Acids Research
Toolkit/CRY2-CIB1 light-inducible transcription system
CRY2-CIB1 light-inducible transcription system
Also known as: CRY2 and CIB1 system
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The CRY2-CIB1 light-inducible transcription system is a multi-component optogenetic switch built from Arabidopsis cryptochrome 2 (CRY2) and CIB1 that drives protein expression by stimulating transcription in response to light. In mammalian cells, it uses light-triggered CRY2-CIB1 interaction to regulate gene expression and can also produce light-dependent redistribution of CRY2-tethered proteins within the nucleus.
Usefulness & Problems
Why this is useful
This system is useful for optical control of transcription, enabling precise regulation of gene expression in mammalian cells. Source literature further states that related CRY2-based tools enable precise bidirectional control of gene expression across a variety of cells and model systems.
Source:
These tools will allow precise, bi-directional control of gene expression in a variety of cells and model systems.
Source:
a system using CRY2 and a LOV-fused degron to simultaneously block transcription and deplete protein levels with light
Source:
a system using CRY2 and CIB1 to induce protein expression with light through stimulation of transcription
Problem solved
It addresses the problem of controlling transgene expression with light rather than constitutive or chemically induced inputs. Specifically, it provides a way to couple illumination to transcriptional activation through CRY2-CIB1 interaction.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
light-dependent subcellular redistributionlight-induced heterodimerizationtranscriptional activationTechniques
No technique tags yet.
Target processes
transcriptionInput: Light
Implementation Constraints
Implementation requires a multi-component genetic design using CRY2 and CIB1 fusion proteins to couple light exposure to transcriptional output. In mammalian cells, construct architecture appears important because light-dependent nuclear clearing was observed for CRY2-tethered proteins and depended on the presence of a dimerization domain in CRY2-fused transcriptional activators.
The supplied evidence is limited to one source focused on mammalian cells, so validation breadth across organisms, promoters, and payload genes is not established here. The same study reports a nuclear clearing phenotype that depended on a dimerization domain in CRY2-fused transcriptional activators, indicating construct-dependent behavior that may complicate interpretation or implementation.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
These tools enable precise bidirectional control of gene expression in a variety of cells and model systems.
These tools will allow precise, bi-directional control of gene expression in a variety of cells and model systems.
These tools enable precise bidirectional control of gene expression in a variety of cells and model systems.
These tools will allow precise, bi-directional control of gene expression in a variety of cells and model systems.
These tools enable precise bidirectional control of gene expression in a variety of cells and model systems.
These tools will allow precise, bi-directional control of gene expression in a variety of cells and model systems.
These tools enable precise bidirectional control of gene expression in a variety of cells and model systems.
These tools will allow precise, bi-directional control of gene expression in a variety of cells and model systems.
These tools enable precise bidirectional control of gene expression in a variety of cells and model systems.
These tools will allow precise, bi-directional control of gene expression in a variety of cells and model systems.
These tools enable precise bidirectional control of gene expression in a variety of cells and model systems.
These tools will allow precise, bi-directional control of gene expression in a variety of cells and model systems.
These tools enable precise bidirectional control of gene expression in a variety of cells and model systems.
These tools will allow precise, bi-directional control of gene expression in a variety of cells and model systems.
CRY2 and CIB1 interact upon light illumination.
CRY2 and CIB1, Arabidopsis proteins that interact upon light illumination
The nuclear clearing phenotype depended on the presence of a dimerization domain in CRY2-fused transcriptional activators.
The nuclear clearing phenotype was dependent on the presence of a dimerization domain contained within the CRY2-fused transcriptional activators.
In mammalian cells, CRY2-tethered proteins showed light-dependent redistribution and clearing within the nucleus.
While adopting this approach to regulate transcription in mammalian cells, we observed light-dependent redistribution and clearing of CRY2-tethered proteins within the nucleus.
A CRY2-CIB1 system was developed to induce protein expression with light through stimulation of transcription.
a system using CRY2 and CIB1 to induce protein expression with light through stimulation of transcription
A CRY2-CIB1 system was developed to induce protein expression with light through stimulation of transcription.
a system using CRY2 and CIB1 to induce protein expression with light through stimulation of transcription
A CRY2-CIB1 system was developed to induce protein expression with light through stimulation of transcription.
a system using CRY2 and CIB1 to induce protein expression with light through stimulation of transcription
A CRY2-CIB1 system was developed to induce protein expression with light through stimulation of transcription.
a system using CRY2 and CIB1 to induce protein expression with light through stimulation of transcription
A CRY2-CIB1 system was developed to induce protein expression with light through stimulation of transcription.
a system using CRY2 and CIB1 to induce protein expression with light through stimulation of transcription
A CRY2-CIB1 system was developed to induce protein expression with light through stimulation of transcription.
a system using CRY2 and CIB1 to induce protein expression with light through stimulation of transcription
A CRY2-CIB1 system was developed to induce protein expression with light through stimulation of transcription.
a system using CRY2 and CIB1 to induce protein expression with light through stimulation of transcription
A CRY2 plus LOV-fused degron system was developed to simultaneously block transcription and deplete protein levels with light.
a system using CRY2 and a LOV-fused degron to simultaneously block transcription and deplete protein levels with light
A CRY2 plus LOV-fused degron system was developed to simultaneously block transcription and deplete protein levels with light.
a system using CRY2 and a LOV-fused degron to simultaneously block transcription and deplete protein levels with light
A CRY2 plus LOV-fused degron system was developed to simultaneously block transcription and deplete protein levels with light.
a system using CRY2 and a LOV-fused degron to simultaneously block transcription and deplete protein levels with light
A CRY2 plus LOV-fused degron system was developed to simultaneously block transcription and deplete protein levels with light.
a system using CRY2 and a LOV-fused degron to simultaneously block transcription and deplete protein levels with light
A CRY2 plus LOV-fused degron system was developed to simultaneously block transcription and deplete protein levels with light.
a system using CRY2 and a LOV-fused degron to simultaneously block transcription and deplete protein levels with light
A CRY2 plus LOV-fused degron system was developed to simultaneously block transcription and deplete protein levels with light.
a system using CRY2 and a LOV-fused degron to simultaneously block transcription and deplete protein levels with light
A CRY2 plus LOV-fused degron system was developed to simultaneously block transcription and deplete protein levels with light.
a system using CRY2 and a LOV-fused degron to simultaneously block transcription and deplete protein levels with light
Approval Evidence
2 sources4 linked approval claimsfirst-pass slug cry2-cib1-light-inducible-transcription-system
a system using CRY2 and CIB1 to induce protein expression with light through stimulation of transcription
Source:
we report newly optimized optogenetic tools to induce transcription with light in mammalian cells ... as well as CRY2/CIB1. In addition, we have improved the CRY2/CIB1-based light-inducible transcription with split construct optimization.
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application scopesupports
The optimized FKF1/GI- and CRY2/CIB1-based systems are presented as widely applicable for light-dependent control of transcription in mammalian cells.
The improvements regarding the FKF1/GI- and CRY2/CIB1-based systems will be widely applicable for the light-dependent control of transcription in mammalian cells.
Source:
capability statementsupports
These tools enable precise bidirectional control of gene expression in a variety of cells and model systems.
These tools will allow precise, bi-directional control of gene expression in a variety of cells and model systems.
Source:
tool functionsupports
A CRY2-CIB1 system was developed to induce protein expression with light through stimulation of transcription.
a system using CRY2 and CIB1 to induce protein expression with light through stimulation of transcription
Source:
tool optimizationsupports
CRY2/CIB1-based light-inducible transcription was improved by split construct optimization in mammalian cells.
In addition, we have improved the CRY2/CIB1-based light-inducible transcription with split construct optimization.
Source:
Comparisons
Source-backed strengths
A key strength is that CRY2 and CIB1 interact upon light illumination, providing a direct optogenetic input-output link for transcriptional control. The cited study also reports light-dependent nuclear redistribution and clearing of CRY2-tethered proteins in mammalian cells, indicating an additional light-responsive behavior that can influence system function.
Ranked Citations
- 1.