Source 1review2024Journal of Molecular Biology
Toolkit/CRY2clust
CRY2clust
Also known as: CRY2clust
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
CRY2clust is an engineered CRY2-based optogenetic module that drives blue light-dependent homo-oligomerization and clustering of fused target proteins. It was reported as a new CRY2 variant that enables rapid and efficient protein clustering with spatiotemporal optical control.
Usefulness & Problems
Why this is useful
CRY2clust is useful for optically inducing protein clustering and homo-oligomerization in a controlled manner. The available evidence indicates that it was developed as an advance over previously reported optogenetic tools and can be implemented using diverse fluorescent proteins together with a very short peptide.
Source:
cryptochrome 2 (CRY2) from Arabidopsis thaliana has been recently utilized for blue light-dependent spatiotemporal control of protein homo-oligomerization
Problem solved
CRY2clust addresses the need for a light-responsive module that can rapidly and efficiently induce target protein homo-oligomerization. It specifically helps solve the challenge of achieving optogenetic protein clustering with improved versatility relative to earlier tools.
Problem links
Need precise spatiotemporal control with light input
DerivedCRY2clust is an engineered CRY2-based optogenetic module for blue light-dependent homo-oligomerization of target proteins. It was introduced as a new CRY2 variant that induces rapid and efficient clustering and supports spatiotemporal control of protein oligomerization.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
light-dependent homo-oligomerizationlight-dependent homo-oligomerizationOligomerizationOligomerizationprotein clusteringprotein clusteringTarget processes
No target processes tagged yet.
Input: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: multi component delivery burdenimplementation constraint: spectral hardware requirementoperating role: actuatorswitch architecture: multi component
The design reportedly employs CRY2 together with diverse fluorescent proteins and an extremely short peptide to achieve target protein homo-oligomerization. The input modality is light, specifically blue light, but the provided evidence does not specify construct architecture, expression system, or cofactor requirements.
The supplied evidence does not provide quantitative performance metrics, illumination parameters, reversibility kinetics, or cell-type-specific validation details. Independent replication is not documented in the provided material.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Source 2primary paper2017Nature Communications
Ranked Claims
CRY2clust showed advancement and versatility when compared against previously reported optogenetic tools.
we demonstrate advancement and versatility of CRY2clust by comparing against previously reported optogenetic tools
CRY2clust showed advancement and versatility when compared against previously reported optogenetic tools.
we demonstrate advancement and versatility of CRY2clust by comparing against previously reported optogenetic tools
CRY2clust showed advancement and versatility when compared against previously reported optogenetic tools.
we demonstrate advancement and versatility of CRY2clust by comparing against previously reported optogenetic tools
CRY2clust showed advancement and versatility when compared against previously reported optogenetic tools.
we demonstrate advancement and versatility of CRY2clust by comparing against previously reported optogenetic tools
CRY2clust showed advancement and versatility when compared against previously reported optogenetic tools.
we demonstrate advancement and versatility of CRY2clust by comparing against previously reported optogenetic tools
CRY2clust showed advancement and versatility when compared against previously reported optogenetic tools.
we demonstrate advancement and versatility of CRY2clust by comparing against previously reported optogenetic tools
CRY2clust showed advancement and versatility when compared against previously reported optogenetic tools.
we demonstrate advancement and versatility of CRY2clust by comparing against previously reported optogenetic tools
CRY2clust showed advancement and versatility when compared against previously reported optogenetic tools.
we demonstrate advancement and versatility of CRY2clust by comparing against previously reported optogenetic tools
CRY2clust showed advancement and versatility when compared against previously reported optogenetic tools.
we demonstrate advancement and versatility of CRY2clust by comparing against previously reported optogenetic tools
CRY2clust showed advancement and versatility when compared against previously reported optogenetic tools.
we demonstrate advancement and versatility of CRY2clust by comparing against previously reported optogenetic tools
CRY2clust showed advancement and versatility when compared against previously reported optogenetic tools.
we demonstrate advancement and versatility of CRY2clust by comparing against previously reported optogenetic tools
CRY2clust showed advancement and versatility when compared against previously reported optogenetic tools.
we demonstrate advancement and versatility of CRY2clust by comparing against previously reported optogenetic tools
CRY2clust showed advancement and versatility when compared against previously reported optogenetic tools.
we demonstrate advancement and versatility of CRY2clust by comparing against previously reported optogenetic tools
CRY2clust showed advancement and versatility when compared against previously reported optogenetic tools.
we demonstrate advancement and versatility of CRY2clust by comparing against previously reported optogenetic tools
CRY2clust showed advancement and versatility when compared against previously reported optogenetic tools.
we demonstrate advancement and versatility of CRY2clust by comparing against previously reported optogenetic tools
CRY2clust showed advancement and versatility when compared against previously reported optogenetic tools.
we demonstrate advancement and versatility of CRY2clust by comparing against previously reported optogenetic tools
CRY2clust showed advancement and versatility when compared against previously reported optogenetic tools.
we demonstrate advancement and versatility of CRY2clust by comparing against previously reported optogenetic tools
CRY2clust achieves target protein homo-oligomerization by employing diverse fluorescent proteins and an extremely short peptide.
by employing diverse fluorescent proteins and an extremely short peptide
CRY2clust achieves target protein homo-oligomerization by employing diverse fluorescent proteins and an extremely short peptide.
by employing diverse fluorescent proteins and an extremely short peptide
CRY2clust achieves target protein homo-oligomerization by employing diverse fluorescent proteins and an extremely short peptide.
by employing diverse fluorescent proteins and an extremely short peptide
CRY2clust achieves target protein homo-oligomerization by employing diverse fluorescent proteins and an extremely short peptide.
by employing diverse fluorescent proteins and an extremely short peptide
CRY2clust achieves target protein homo-oligomerization by employing diverse fluorescent proteins and an extremely short peptide.
by employing diverse fluorescent proteins and an extremely short peptide
CRY2clust achieves target protein homo-oligomerization by employing diverse fluorescent proteins and an extremely short peptide.
by employing diverse fluorescent proteins and an extremely short peptide
CRY2clust achieves target protein homo-oligomerization by employing diverse fluorescent proteins and an extremely short peptide.
by employing diverse fluorescent proteins and an extremely short peptide
CRY2clust achieves target protein homo-oligomerization by employing diverse fluorescent proteins and an extremely short peptide.
by employing diverse fluorescent proteins and an extremely short peptide
CRY2clust achieves target protein homo-oligomerization by employing diverse fluorescent proteins and an extremely short peptide.
by employing diverse fluorescent proteins and an extremely short peptide
CRY2clust achieves target protein homo-oligomerization by employing diverse fluorescent proteins and an extremely short peptide.
by employing diverse fluorescent proteins and an extremely short peptide
CRY2clust achieves target protein homo-oligomerization by employing diverse fluorescent proteins and an extremely short peptide.
by employing diverse fluorescent proteins and an extremely short peptide
CRY2clust achieves target protein homo-oligomerization by employing diverse fluorescent proteins and an extremely short peptide.
by employing diverse fluorescent proteins and an extremely short peptide
CRY2clust achieves target protein homo-oligomerization by employing diverse fluorescent proteins and an extremely short peptide.
by employing diverse fluorescent proteins and an extremely short peptide
CRY2clust achieves target protein homo-oligomerization by employing diverse fluorescent proteins and an extremely short peptide.
by employing diverse fluorescent proteins and an extremely short peptide
CRY2clust achieves target protein homo-oligomerization by employing diverse fluorescent proteins and an extremely short peptide.
by employing diverse fluorescent proteins and an extremely short peptide
CRY2clust achieves target protein homo-oligomerization by employing diverse fluorescent proteins and an extremely short peptide.
by employing diverse fluorescent proteins and an extremely short peptide
CRY2clust achieves target protein homo-oligomerization by employing diverse fluorescent proteins and an extremely short peptide.
by employing diverse fluorescent proteins and an extremely short peptide
CRY2clust is a new CRY2 module that induces rapid and efficient homo-oligomerization of target proteins.
introduce a new CRY2 module, named ''CRY2clust'', to induce rapid and efficient homo-oligomerization of target proteins
CRY2clust is a new CRY2 module that induces rapid and efficient homo-oligomerization of target proteins.
introduce a new CRY2 module, named ''CRY2clust'', to induce rapid and efficient homo-oligomerization of target proteins
CRY2clust is a new CRY2 module that induces rapid and efficient homo-oligomerization of target proteins.
introduce a new CRY2 module, named ''CRY2clust'', to induce rapid and efficient homo-oligomerization of target proteins
CRY2clust is a new CRY2 module that induces rapid and efficient homo-oligomerization of target proteins.
introduce a new CRY2 module, named ''CRY2clust'', to induce rapid and efficient homo-oligomerization of target proteins
CRY2clust is a new CRY2 module that induces rapid and efficient homo-oligomerization of target proteins.
introduce a new CRY2 module, named ''CRY2clust'', to induce rapid and efficient homo-oligomerization of target proteins
CRY2clust is a new CRY2 module that induces rapid and efficient homo-oligomerization of target proteins.
introduce a new CRY2 module, named ''CRY2clust'', to induce rapid and efficient homo-oligomerization of target proteins
CRY2clust is a new CRY2 module that induces rapid and efficient homo-oligomerization of target proteins.
introduce a new CRY2 module, named ''CRY2clust'', to induce rapid and efficient homo-oligomerization of target proteins
CRY2clust is a new CRY2 module that induces rapid and efficient homo-oligomerization of target proteins.
introduce a new CRY2 module, named ''CRY2clust'', to induce rapid and efficient homo-oligomerization of target proteins
CRY2clust is a new CRY2 module that induces rapid and efficient homo-oligomerization of target proteins.
introduce a new CRY2 module, named ''CRY2clust'', to induce rapid and efficient homo-oligomerization of target proteins
CRY2clust is a new CRY2 module that induces rapid and efficient homo-oligomerization of target proteins.
introduce a new CRY2 module, named ''CRY2clust'', to induce rapid and efficient homo-oligomerization of target proteins
CRY2clust is a new CRY2 module that induces rapid and efficient homo-oligomerization of target proteins.
introduce a new CRY2 module, named ''CRY2clust'', to induce rapid and efficient homo-oligomerization of target proteins
CRY2clust is a new CRY2 module that induces rapid and efficient homo-oligomerization of target proteins.
introduce a new CRY2 module, named ''CRY2clust'', to induce rapid and efficient homo-oligomerization of target proteins
CRY2clust is a new CRY2 module that induces rapid and efficient homo-oligomerization of target proteins.
introduce a new CRY2 module, named ''CRY2clust'', to induce rapid and efficient homo-oligomerization of target proteins
CRY2clust is a new CRY2 module that induces rapid and efficient homo-oligomerization of target proteins.
introduce a new CRY2 module, named ''CRY2clust'', to induce rapid and efficient homo-oligomerization of target proteins
CRY2clust is a new CRY2 module that induces rapid and efficient homo-oligomerization of target proteins.
introduce a new CRY2 module, named ''CRY2clust'', to induce rapid and efficient homo-oligomerization of target proteins
CRY2clust is a new CRY2 module that induces rapid and efficient homo-oligomerization of target proteins.
introduce a new CRY2 module, named ''CRY2clust'', to induce rapid and efficient homo-oligomerization of target proteins
CRY2clust is a new CRY2 module that induces rapid and efficient homo-oligomerization of target proteins.
introduce a new CRY2 module, named ''CRY2clust'', to induce rapid and efficient homo-oligomerization of target proteins
CRY2 from Arabidopsis thaliana can be used for blue light-dependent spatiotemporal control of protein homo-oligomerization.
cryptochrome 2 (CRY2) from Arabidopsis thaliana has been recently utilized for blue light-dependent spatiotemporal control of protein homo-oligomerization
CRY2 from Arabidopsis thaliana can be used for blue light-dependent spatiotemporal control of protein homo-oligomerization.
cryptochrome 2 (CRY2) from Arabidopsis thaliana has been recently utilized for blue light-dependent spatiotemporal control of protein homo-oligomerization
CRY2 from Arabidopsis thaliana can be used for blue light-dependent spatiotemporal control of protein homo-oligomerization.
cryptochrome 2 (CRY2) from Arabidopsis thaliana has been recently utilized for blue light-dependent spatiotemporal control of protein homo-oligomerization
CRY2 from Arabidopsis thaliana can be used for blue light-dependent spatiotemporal control of protein homo-oligomerization.
cryptochrome 2 (CRY2) from Arabidopsis thaliana has been recently utilized for blue light-dependent spatiotemporal control of protein homo-oligomerization
CRY2 from Arabidopsis thaliana can be used for blue light-dependent spatiotemporal control of protein homo-oligomerization.
cryptochrome 2 (CRY2) from Arabidopsis thaliana has been recently utilized for blue light-dependent spatiotemporal control of protein homo-oligomerization
The authors identified important determinants for efficient CRY2 clustering.
Here, we identify important determinants for efficient cryptochrome 2 clustering
The authors identified important determinants for efficient CRY2 clustering.
Here, we identify important determinants for efficient cryptochrome 2 clustering
The authors identified important determinants for efficient CRY2 clustering.
Here, we identify important determinants for efficient cryptochrome 2 clustering
The authors identified important determinants for efficient CRY2 clustering.
Here, we identify important determinants for efficient cryptochrome 2 clustering
The authors identified important determinants for efficient CRY2 clustering.
Here, we identify important determinants for efficient cryptochrome 2 clustering
This work expands the optogenetic clustering toolbox and provides a guideline for designing new CRY2-based optogenetic modules.
Our work not only expands the optogenetic clustering toolbox but also provides a guideline for designing CRY2-based new optogenetic modules.
This work expands the optogenetic clustering toolbox and provides a guideline for designing new CRY2-based optogenetic modules.
Our work not only expands the optogenetic clustering toolbox but also provides a guideline for designing CRY2-based new optogenetic modules.
This work expands the optogenetic clustering toolbox and provides a guideline for designing new CRY2-based optogenetic modules.
Our work not only expands the optogenetic clustering toolbox but also provides a guideline for designing CRY2-based new optogenetic modules.
This work expands the optogenetic clustering toolbox and provides a guideline for designing new CRY2-based optogenetic modules.
Our work not only expands the optogenetic clustering toolbox but also provides a guideline for designing CRY2-based new optogenetic modules.
This work expands the optogenetic clustering toolbox and provides a guideline for designing new CRY2-based optogenetic modules.
Our work not only expands the optogenetic clustering toolbox but also provides a guideline for designing CRY2-based new optogenetic modules.
This work expands the optogenetic clustering toolbox and provides a guideline for designing new CRY2-based optogenetic modules.
Our work not only expands the optogenetic clustering toolbox but also provides a guideline for designing CRY2-based new optogenetic modules.
This work expands the optogenetic clustering toolbox and provides a guideline for designing new CRY2-based optogenetic modules.
Our work not only expands the optogenetic clustering toolbox but also provides a guideline for designing CRY2-based new optogenetic modules.
This work expands the optogenetic clustering toolbox and provides a guideline for designing new CRY2-based optogenetic modules.
Our work not only expands the optogenetic clustering toolbox but also provides a guideline for designing CRY2-based new optogenetic modules.
This work expands the optogenetic clustering toolbox and provides a guideline for designing new CRY2-based optogenetic modules.
Our work not only expands the optogenetic clustering toolbox but also provides a guideline for designing CRY2-based new optogenetic modules.
This work expands the optogenetic clustering toolbox and provides a guideline for designing new CRY2-based optogenetic modules.
Our work not only expands the optogenetic clustering toolbox but also provides a guideline for designing CRY2-based new optogenetic modules.
This work expands the optogenetic clustering toolbox and provides a guideline for designing new CRY2-based optogenetic modules.
Our work not only expands the optogenetic clustering toolbox but also provides a guideline for designing CRY2-based new optogenetic modules.
This work expands the optogenetic clustering toolbox and provides a guideline for designing new CRY2-based optogenetic modules.
Our work not only expands the optogenetic clustering toolbox but also provides a guideline for designing CRY2-based new optogenetic modules.
This work expands the optogenetic clustering toolbox and provides a guideline for designing new CRY2-based optogenetic modules.
Our work not only expands the optogenetic clustering toolbox but also provides a guideline for designing CRY2-based new optogenetic modules.
This work expands the optogenetic clustering toolbox and provides a guideline for designing new CRY2-based optogenetic modules.
Our work not only expands the optogenetic clustering toolbox but also provides a guideline for designing CRY2-based new optogenetic modules.
This work expands the optogenetic clustering toolbox and provides a guideline for designing new CRY2-based optogenetic modules.
Our work not only expands the optogenetic clustering toolbox but also provides a guideline for designing CRY2-based new optogenetic modules.
This work expands the optogenetic clustering toolbox and provides a guideline for designing new CRY2-based optogenetic modules.
Our work not only expands the optogenetic clustering toolbox but also provides a guideline for designing CRY2-based new optogenetic modules.
This work expands the optogenetic clustering toolbox and provides a guideline for designing new CRY2-based optogenetic modules.
Our work not only expands the optogenetic clustering toolbox but also provides a guideline for designing CRY2-based new optogenetic modules.
Approval Evidence
2 sources4 linked approval claimsfirst-pass slug cry2clust
Additional strong leads include foundational actuator-engineering papers for CRY2olig/CRY2clust.
Source:
introduce a new CRY2 module, named ''CRY2clust'', to induce rapid and efficient homo-oligomerization of target proteins
Source:
comparative performancesupports
CRY2clust showed advancement and versatility when compared against previously reported optogenetic tools.
we demonstrate advancement and versatility of CRY2clust by comparing against previously reported optogenetic tools
Source:
design strategysupports
CRY2clust achieves target protein homo-oligomerization by employing diverse fluorescent proteins and an extremely short peptide.
by employing diverse fluorescent proteins and an extremely short peptide
Source:
engineering resultsupports
CRY2clust is a new CRY2 module that induces rapid and efficient homo-oligomerization of target proteins.
introduce a new CRY2 module, named ''CRY2clust'', to induce rapid and efficient homo-oligomerization of target proteins
Source:
toolbox expansionsupports
This work expands the optogenetic clustering toolbox and provides a guideline for designing new CRY2-based optogenetic modules.
Our work not only expands the optogenetic clustering toolbox but also provides a guideline for designing CRY2-based new optogenetic modules.
Source:
Comparisons
Source-backed strengths
The reported strengths are rapid and efficient induction of target protein homo-oligomerization. Source claims also state that CRY2clust showed advancement and versatility compared with previously reported optogenetic tools.
Source:
we demonstrate advancement and versatility of CRY2clust by comparing against previously reported optogenetic tools
Source:
introduce a new CRY2 module, named ''CRY2clust'', to induce rapid and efficient homo-oligomerization of target proteins
Compared with AQTrip EL222 variant
CRY2clust and AQTrip EL222 variant address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: oligomerization; same primary input modality: light
Compared with OptoLoop
CRY2clust and OptoLoop address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: oligomerization; same primary input modality: light
Compared with optoRET
CRY2clust and optoRET address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: oligomerization; same primary input modality: light
Ranked Citations
- 1.
Extracted from this source document.
- 2.