Source 1primary paper2022TSpace
Toolkit/cyanobacteriochromes
cyanobacteriochromes
Taxonomy: Mechanism Branch / Component. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Cyanobacteriochromes are photoswitchable protein domains from cyanobacteria that sense light across a broad spectral range from the UV to the near infra-red. In the cited 2022 work, they were engineered into light-inducible dimer pairs that support orthogonal control with red, green, and blue light.
Usefulness & Problems
Why this is useful
These domains are useful as optogenetic components because their native spectral diversity enables development of multichromatic light-responsive tools. The cited study specifically positions cyanobacteriochrome-derived dimer pairs as a route to orthogonal control of biological processes with distinct visible-light inputs.
Source:
permitting orthogonal control of biological processes with red-, green-, and blue-light
Source:
New light inducible dimer pairs from these photoswitchable proteins are developed via directed evolution approaches
Problem solved
This tool helps address the challenge of achieving orthogonal optogenetic control with multiple wavelengths rather than relying on a single light channel. The cited work specifically developed cyanobacteriochrome-based dimer pairs for red-, green-, and blue-light control.
Source:
New light inducible dimer pairs from these photoswitchable proteins are developed via directed evolution approaches
Published Workflows
Objective: Develop near-infrared fluorescent proteins and biosensors from bacterial photoreceptor-derived templates and guide selection of existing probes for imaging use.
Why it works: The review frames naturally NIR-absorbing bacterial photoreceptors as starting templates, then applies molecular evolution to generate fluorescent proteins and biosensors, followed by comparison of resulting phenotypes and consideration of imaging applications.
use of bacterial photoreceptor scaffolds with near-infrared absorbance as molecular templatesengineering of fluorescent proteins and biosensors from natural photoreceptor familiesmolecular evolutioncomparative phenotype analysisprobe selection
Stages
- 1.Source scaffold characterization(functional_characterization)
The review indicates that understanding source photoreceptor families is the basis for choosing and engineering NIR probe templates.
Selection: Structures, photochemical properties, and molecular functions of several families of bacterial photoreceptors
- 2.Molecular evolution-based probe development(library_design)
After identifying suitable natural templates, engineering is used to convert them into NIR fluorescent proteins and biosensors.
Selection: Molecular evolution approaches to develop NIR fluorescent proteins and biosensors
- 3.Comparative phenotype assessment of engineered probe classes(secondary_characterization)
The review explicitly compares current BphP-based NIR FPs with CBCR- and APC-derived alternatives to inform probe choice and future engineering.
Selection: Phenotypes of current BphP-based NIR FPs and comparison with FPs derived from CBCRs and APCs
- 4.Application-oriented imaging evaluation and selection guidance(confirmatory_validation)
Application context is used to guide selection of existing NIR fluorescent proteins and motivate engineering of improved probes.
Selection: Imaging applications in live cells and in vivo and guidelines for selection of existing NIR FPs
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Component: A low-level protein part used inside a larger architecture that realizes a mechanism.
Mechanisms
HeterodimerizationTechniques
Directed EvolutionTarget processes
No target processes tagged yet.
Input: Light
Implementation Constraints
The available evidence indicates that the reported tools were created from cyanobacteriochrome photoswitchable proteins using directed evolution. However, the provided material does not describe construct architecture, chromophore requirements, expression conditions, or delivery considerations.
The provided evidence does not specify the individual cyanobacteriochrome variants, binding partners, kinetics, dynamic range, or host-system validation details. It also does not establish performance outside the cited study or compare these tools quantitatively to other optogenetic systems.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The developed light inducible dimer pairs permit orthogonal control of biological processes with red-, green-, and blue-light.
permitting orthogonal control of biological processes with red-, green-, and blue-light
The developed light inducible dimer pairs permit orthogonal control of biological processes with red-, green-, and blue-light.
permitting orthogonal control of biological processes with red-, green-, and blue-light
The developed light inducible dimer pairs permit orthogonal control of biological processes with red-, green-, and blue-light.
permitting orthogonal control of biological processes with red-, green-, and blue-light
The developed light inducible dimer pairs permit orthogonal control of biological processes with red-, green-, and blue-light.
permitting orthogonal control of biological processes with red-, green-, and blue-light
The developed light inducible dimer pairs permit orthogonal control of biological processes with red-, green-, and blue-light.
permitting orthogonal control of biological processes with red-, green-, and blue-light
The developed light inducible dimer pairs permit orthogonal control of biological processes with red-, green-, and blue-light.
permitting orthogonal control of biological processes with red-, green-, and blue-light
The developed light inducible dimer pairs permit orthogonal control of biological processes with red-, green-, and blue-light.
permitting orthogonal control of biological processes with red-, green-, and blue-light
Cyanobacteriochromes respond to a wide range of wavelengths from the UV to the near infra-red.
Cyanobacteriochromes, photoswitchable proteins from cyanobacteria, respond to a wide range of wavelengths from the UV to the near infra-red.
Cyanobacteriochromes respond to a wide range of wavelengths from the UV to the near infra-red.
Cyanobacteriochromes, photoswitchable proteins from cyanobacteria, respond to a wide range of wavelengths from the UV to the near infra-red.
Cyanobacteriochromes respond to a wide range of wavelengths from the UV to the near infra-red.
Cyanobacteriochromes, photoswitchable proteins from cyanobacteria, respond to a wide range of wavelengths from the UV to the near infra-red.
Cyanobacteriochromes respond to a wide range of wavelengths from the UV to the near infra-red.
Cyanobacteriochromes, photoswitchable proteins from cyanobacteria, respond to a wide range of wavelengths from the UV to the near infra-red.
Cyanobacteriochromes respond to a wide range of wavelengths from the UV to the near infra-red.
Cyanobacteriochromes, photoswitchable proteins from cyanobacteria, respond to a wide range of wavelengths from the UV to the near infra-red.
Cyanobacteriochromes respond to a wide range of wavelengths from the UV to the near infra-red.
Cyanobacteriochromes, photoswitchable proteins from cyanobacteria, respond to a wide range of wavelengths from the UV to the near infra-red.
Cyanobacteriochromes respond to a wide range of wavelengths from the UV to the near infra-red.
Cyanobacteriochromes, photoswitchable proteins from cyanobacteria, respond to a wide range of wavelengths from the UV to the near infra-red.
New light inducible dimer pairs were developed from cyanobacteriochrome photoswitchable proteins via directed evolution approaches.
New light inducible dimer pairs from these photoswitchable proteins are developed via directed evolution approaches
New light inducible dimer pairs were developed from cyanobacteriochrome photoswitchable proteins via directed evolution approaches.
New light inducible dimer pairs from these photoswitchable proteins are developed via directed evolution approaches
New light inducible dimer pairs were developed from cyanobacteriochrome photoswitchable proteins via directed evolution approaches.
New light inducible dimer pairs from these photoswitchable proteins are developed via directed evolution approaches
New light inducible dimer pairs were developed from cyanobacteriochrome photoswitchable proteins via directed evolution approaches.
New light inducible dimer pairs from these photoswitchable proteins are developed via directed evolution approaches
New light inducible dimer pairs were developed from cyanobacteriochrome photoswitchable proteins via directed evolution approaches.
New light inducible dimer pairs from these photoswitchable proteins are developed via directed evolution approaches
New light inducible dimer pairs were developed from cyanobacteriochrome photoswitchable proteins via directed evolution approaches.
New light inducible dimer pairs from these photoswitchable proteins are developed via directed evolution approaches
New light inducible dimer pairs were developed from cyanobacteriochrome photoswitchable proteins via directed evolution approaches.
New light inducible dimer pairs from these photoswitchable proteins are developed via directed evolution approaches
Approval Evidence
1 source2 linked approval claimsfirst-pass slug cyanobacteriochromes
Cyanobacteriochromes, photoswitchable proteins from cyanobacteria, respond to a wide range of wavelengths from the UV to the near infra-red.
Source:
spectral rangesupports
Cyanobacteriochromes respond to a wide range of wavelengths from the UV to the near infra-red.
Cyanobacteriochromes, photoswitchable proteins from cyanobacteria, respond to a wide range of wavelengths from the UV to the near infra-red.
Source:
tool developmentsupports
New light inducible dimer pairs were developed from cyanobacteriochrome photoswitchable proteins via directed evolution approaches.
New light inducible dimer pairs from these photoswitchable proteins are developed via directed evolution approaches
Source:
Comparisons
Source-backed strengths
A key strength is the broad underlying spectral responsiveness of cyanobacteriochromes, spanning UV to near infra-red. The cited work further reports development of new light-inducible dimer pairs and states that these permit orthogonal control with red, green, and blue light.
Ranked Citations
- 1.