Source 1review2012Brain Research
Toolkit/DAT-Cre
DAT-Cre
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The supplied web research summary explicitly identifies DAT-Cre as a genetic targeting component used in selective dopamine-neuron stimulation and terminal-dynamics studies relevant to this review topic.
Usefulness & Problems
Why this is useful
DAT-Cre is a genetic targeting component used to direct optogenetic tools to dopamine-neuron populations. The supplied summary links it to studies of terminal dopamine dynamics and selective stimulation.; selective targeting of dopamine neurons for optogenetic studies; studies of terminal dopamine dynamics
Source:
DAT-Cre is a genetic targeting component used to direct optogenetic tools to dopamine-neuron populations. The supplied summary links it to studies of terminal dopamine dynamics and selective stimulation.
Source:
selective targeting of dopamine neurons for optogenetic studies
Source:
studies of terminal dopamine dynamics
Problem solved
It helps achieve dopamine-neuron-selective expression needed for causal optogenetic experiments.; enables targeted opsin expression in dopamine-neuron populations
Source:
It helps achieve dopamine-neuron-selective expression needed for causal optogenetic experiments.
Source:
enables targeted opsin expression in dopamine-neuron populations
Problem links
enables targeted opsin expression in dopamine-neuron populations
LiteratureIt helps achieve dopamine-neuron-selective expression needed for causal optogenetic experiments.
Source:
It helps achieve dopamine-neuron-selective expression needed for causal optogenetic experiments.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Mechanisms
cre-lox recombinationTechniques
No technique tags yet.
Target processes
recombinationInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: payload burdenimplementation constraint: spectral hardware requirementoperating role: regulator
It requires a Cre-dependent payload and the corresponding transgenic or recombinase-based targeting setup.; requires compatible Cre-dependent expression strategy
The provided evidence does not specify how it compares with TH-Cre in specificity or coverage.; the provided payload does not include review-level comparison against TH-Cre or other targeting strategies
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
In source material connected to this review, ChR2 and NpHR are explicit optogenetic actuators used to interrogate dopamine circuits, while FSCV is an explicit paired measurement method for dopamine release dynamics.
Explicitly supported related components/tools include ChR2, NpHR, fast-scan cyclic voltammetry (FSCV), TH-Cre, DAT-Cre, and recombinase-driver rat lines.
Selective genetic targeting is a central enabling component in dopamine optogenetics, with TH-Cre, DAT-Cre, and recombinase-driver rat lines identified as relevant targeting tools in source material connected to this review.
Explicitly supported related components/tools include ChR2, NpHR, fast-scan cyclic voltammetry (FSCV), TH-Cre, DAT-Cre, and recombinase-driver rat lines.
Approval Evidence
1 source1 linked approval claimfirst-pass slug dat-cre
The supplied web research summary explicitly identifies DAT-Cre as a genetic targeting component used in selective dopamine-neuron stimulation and terminal-dynamics studies relevant to this review topic.
Source:
targeting summarysupports
Selective genetic targeting is a central enabling component in dopamine optogenetics, with TH-Cre, DAT-Cre, and recombinase-driver rat lines identified as relevant targeting tools in source material connected to this review.
Explicitly supported related components/tools include ChR2, NpHR, fast-scan cyclic voltammetry (FSCV), TH-Cre, DAT-Cre, and recombinase-driver rat lines.
Source:
Comparisons
Source-stated alternatives
The summary mentions TH-Cre and recombinase-driver rat lines as related targeting approaches.
Source:
The summary mentions TH-Cre and recombinase-driver rat lines as related targeting approaches.
Source-backed strengths
used in studies of selective dopamine-neuron stimulation and terminal dynamics
Source:
used in studies of selective dopamine-neuron stimulation and terminal dynamics
Compared with recombinase-driver rat lines
The summary mentions TH-Cre and recombinase-driver rat lines as related targeting approaches.
Shared frame: source-stated alternative in extracted literature
Strengths here: used in studies of selective dopamine-neuron stimulation and terminal dynamics.
Relative tradeoffs: the provided payload does not include review-level comparison against TH-Cre or other targeting strategies.
Source:
The summary mentions TH-Cre and recombinase-driver rat lines as related targeting approaches.
Compared with TH-Cre
The summary mentions TH-Cre and recombinase-driver rat lines as related targeting approaches.
Shared frame: source-stated alternative in extracted literature
Strengths here: used in studies of selective dopamine-neuron stimulation and terminal dynamics.
Relative tradeoffs: the provided payload does not include review-level comparison against TH-Cre or other targeting strategies.
Source:
The summary mentions TH-Cre and recombinase-driver rat lines as related targeting approaches.
Ranked Citations
- 1.