Source 1primary paper2019
Toolkit/Deg-LITer
Deg-LITer
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Deg-LITer is a multi-component optogenetic gene circuit in which the TetR repressor is fused to a degradation tag through the LOV2 light-sensitive domain. It is part of the LITer toolset for light-controlled regulation in mammalian cells.
Usefulness & Problems
Why this is useful
This design is useful as a light-responsive means to couple a transcriptional regulator to a degradation module in mammalian cells. The available evidence supports its role as a component of an optogenetic regulation platform, but does not provide performance details in the supplied source.
Problem solved
Deg-LITer addresses the need for light-controlled regulation of gene circuits in mammalian cells using a TetR-based architecture linked to a degradation tag. The supplied evidence does not further specify the exact experimental bottleneck or comparative advantage it was designed to overcome.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
DegradationDegradationlight-regulated degradationlight-regulated degradationlov2-based photosensory controllov2-based photosensory controlTechniques
No technique tags yet.
Target processes
degradationInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: multi component delivery burdenimplementation constraint: spectral hardware requirementoperating role: actuatorswitch architecture: multi componentvariant basis: degradation-tag-based
The reported construct contains TetR, a degradation tag, and the LOV2 light-sensitive domain in a fusion architecture. It is described in the context of mammalian cells, but the source excerpt does not specify construct orientation, promoter design, illumination conditions, or required cofactors.
The supplied evidence is limited to circuit composition and does not report activation wavelength, dynamic range, kinetics, leakiness, or target gene outputs. Independent replication and breadth of validation cannot be established from the provided material.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Source 2primary paper2019Nucleic Acids Research
Ranked Claims
The LITer toolset uses TetR fused with either a Tet-Inhibitory peptide or a degradation tag through the LOV2 light-sensitive domain.
We build a toolset of these noise-reducing Light-Inducible Tuner (LITer) gene circuits using the TetR repressor fused with a Tet-Inhibitory peptide (TIP) or a degradation tag through the light-sensitive LOV2 protein domain.
The LITer toolset uses TetR fused with either a Tet-Inhibitory peptide or a degradation tag through the LOV2 light-sensitive domain.
We build a toolset of these noise-reducing Light-Inducible Tuner (LITer) gene circuits using the TetR repressor fused with a Tet-Inhibitory peptide (TIP) or a degradation tag through the light-sensitive LOV2 protein domain.
The LITer toolset uses TetR fused with either a Tet-Inhibitory peptide or a degradation tag through the LOV2 light-sensitive domain.
We build a toolset of these noise-reducing Light-Inducible Tuner (LITer) gene circuits using the TetR repressor fused with a Tet-Inhibitory peptide (TIP) or a degradation tag through the light-sensitive LOV2 protein domain.
The LITer toolset uses TetR fused with either a Tet-Inhibitory peptide or a degradation tag through the LOV2 light-sensitive domain.
We build a toolset of these noise-reducing Light-Inducible Tuner (LITer) gene circuits using the TetR repressor fused with a Tet-Inhibitory peptide (TIP) or a degradation tag through the light-sensitive LOV2 protein domain.
The LITer toolset uses TetR fused with either a Tet-Inhibitory peptide or a degradation tag through the LOV2 light-sensitive domain.
We build a toolset of these noise-reducing Light-Inducible Tuner (LITer) gene circuits using the TetR repressor fused with a Tet-Inhibitory peptide (TIP) or a degradation tag through the light-sensitive LOV2 protein domain.
Approval Evidence
2 sources1 linked approval claimfirst-pass slug deg-liter
We build a toolset of these noise-reducing Light-Inducible Tuner (LITer) gene circuits using the TetR repressor fused with ... a degradation tag through the light-sensitive LOV2 protein domain.
Source:
We build a toolset of these noise-reducing Light-Inducible Tuner (LITer) gene circuits using the TetR repressor fused with ... a degradation tag through the light-sensitive LOV2 protein domain.
Source:
compositionsupports
The LITer toolset uses TetR fused with either a Tet-Inhibitory peptide or a degradation tag through the LOV2 light-sensitive domain.
We build a toolset of these noise-reducing Light-Inducible Tuner (LITer) gene circuits using the TetR repressor fused with a Tet-Inhibitory peptide (TIP) or a degradation tag through the light-sensitive LOV2 protein domain.
Source:
Comparisons
Source-backed strengths
A clear strength is its modular composition: TetR is connected to a degradation tag via the LOV2 photosensory domain, enabling a genetically encoded light-responsive design. The source also places it within a broader LITer toolset for mammalian optogenetics, but no quantitative validation is provided here.
Ranked Citations
- 1.
- 2.
Extracted from this source document.