Source 1primary paper2014Nucleic Acids Research
Toolkit/designer cells engineered for gene switch-driven SEAP expression
designer cells engineered for gene switch-driven SEAP expression
Also known as: designer cells, gene switch-driven expression of the human placental secreted alkaline phosphatase (SEAP)
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
This tool comprises designer mammalian cells engineered to express human placental secreted alkaline phosphatase (SEAP) under control of a benzoate-/vanillate-responsive mammalian gene switch. It functions as a small-molecule-regulated reporter system for monitoring inducible and repressible transgene expression in vitro and in implanted mice.
Usefulness & Problems
Why this is useful
The system is useful for reporting mammalian transgene control by food additive inputs, using secreted SEAP as an accessible output. The cited study also indicates that the benzoate-/vanillate-responsive device is compatible with other transgene control systems and can be incorporated into higher-order control networks.
Source:
we have designed different mammalian gene expression systems that could be induced and repressed by the food additives benzoate and vanillate
Source:
When implanting designer cells engineered for gene switch-driven expression of the human placental secreted alkaline phosphatase (SEAP) into mice, blood SEAP levels of treated animals directly correlated with a benzoate-enriched drinking programme.
Problem solved
This tool addresses the need for a mammalian reporter cell system that links externally supplied small molecules, specifically benzoate and vanillate, to controllable transgene expression. It also supports testing of dual-input antagonistic gene regulation in cultured cells and implanted-cell mouse settings.
Problem links
Need conditional recombination or state switching
DerivedThis tool comprises designer mammalian cells engineered to express human placental secreted alkaline phosphatase (SEAP) under control of a benzoate-/vanillate-responsive mammalian gene switch. It functions as a small-molecule-regulated reporter system for monitoring inducible and repressible transgene expression in vitro and in implanted mice.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Techniques
Computational DesignTarget processes
recombinationImplementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: reporter
The construct places human placental secreted alkaline phosphatase under control of a benzoate-/vanillate-responsive mammalian gene switch in designer mammalian cells. Practical use in the cited work included implantation of the engineered cells into mice and modulation of output through a benzoate-enriched drinking program. Additional implementation details are not available from the supplied evidence.
The provided evidence does not specify the exact switch architecture, promoter design, host cell line, or quantitative performance metrics such as dynamic range or response kinetics. Validation is described for SEAP reporting and implanted-mouse correlation, but broader benchmarking across cell types, payload genes, or long-term performance is not provided here.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Observations
successMouseapplication demo
Inferred from claim c2 during normalization. In mice implanted with designer cells expressing SEAP under gene-switch control, blood SEAP levels directly correlated with a benzoate-enriched drinking program. Derived from claim c2. Quoted text: When implanting designer cells engineered for gene switch-driven expression of the human placental secreted alkaline phosphatase (SEAP) into mice, blood SEAP levels of treated animals directly correlated with a benzoate-enriched drinking programme.
Source:
successMouseapplication demo
Inferred from claim c2 during normalization. In mice implanted with designer cells expressing SEAP under gene-switch control, blood SEAP levels directly correlated with a benzoate-enriched drinking program. Derived from claim c2. Quoted text: When implanting designer cells engineered for gene switch-driven expression of the human placental secreted alkaline phosphatase (SEAP) into mice, blood SEAP levels of treated animals directly correlated with a benzoate-enriched drinking programme.
Source:
successMouseapplication demo
Inferred from claim c2 during normalization. In mice implanted with designer cells expressing SEAP under gene-switch control, blood SEAP levels directly correlated with a benzoate-enriched drinking program. Derived from claim c2. Quoted text: When implanting designer cells engineered for gene switch-driven expression of the human placental secreted alkaline phosphatase (SEAP) into mice, blood SEAP levels of treated animals directly correlated with a benzoate-enriched drinking programme.
Source:
successMouseapplication demo
Inferred from claim c2 during normalization. In mice implanted with designer cells expressing SEAP under gene-switch control, blood SEAP levels directly correlated with a benzoate-enriched drinking program. Derived from claim c2. Quoted text: When implanting designer cells engineered for gene switch-driven expression of the human placental secreted alkaline phosphatase (SEAP) into mice, blood SEAP levels of treated animals directly correlated with a benzoate-enriched drinking programme.
Source:
successMouseapplication demo
Inferred from claim c2 during normalization. In mice implanted with designer cells expressing SEAP under gene-switch control, blood SEAP levels directly correlated with a benzoate-enriched drinking program. Derived from claim c2. Quoted text: When implanting designer cells engineered for gene switch-driven expression of the human placental secreted alkaline phosphatase (SEAP) into mice, blood SEAP levels of treated animals directly correlated with a benzoate-enriched drinking programme.
Source:
successMouseapplication demo
Inferred from claim c2 during normalization. In mice implanted with designer cells expressing SEAP under gene-switch control, blood SEAP levels directly correlated with a benzoate-enriched drinking program. Derived from claim c2. Quoted text: When implanting designer cells engineered for gene switch-driven expression of the human placental secreted alkaline phosphatase (SEAP) into mice, blood SEAP levels of treated animals directly correlated with a benzoate-enriched drinking programme.
Source:
successMouseapplication demo
Inferred from claim c2 during normalization. In mice implanted with designer cells expressing SEAP under gene-switch control, blood SEAP levels directly correlated with a benzoate-enriched drinking program. Derived from claim c2. Quoted text: When implanting designer cells engineered for gene switch-driven expression of the human placental secreted alkaline phosphatase (SEAP) into mice, blood SEAP levels of treated animals directly correlated with a benzoate-enriched drinking programme.
Source:
Supporting Sources
Ranked Claims
The benzoate-/vanillate-responsive device was compatible with other transgene control systems and could be assembled into higher-order control networks.
the benzoate-/vanillate-responsive device was compatible with other transgene control systems and could be assembled into higher-order control networks
The benzoate-/vanillate-responsive device was compatible with other transgene control systems and could be assembled into higher-order control networks.
the benzoate-/vanillate-responsive device was compatible with other transgene control systems and could be assembled into higher-order control networks
The benzoate-/vanillate-responsive device was compatible with other transgene control systems and could be assembled into higher-order control networks.
the benzoate-/vanillate-responsive device was compatible with other transgene control systems and could be assembled into higher-order control networks
The benzoate-/vanillate-responsive device was compatible with other transgene control systems and could be assembled into higher-order control networks.
the benzoate-/vanillate-responsive device was compatible with other transgene control systems and could be assembled into higher-order control networks
The benzoate-/vanillate-responsive device was compatible with other transgene control systems and could be assembled into higher-order control networks.
the benzoate-/vanillate-responsive device was compatible with other transgene control systems and could be assembled into higher-order control networks
The benzoate-/vanillate-responsive device was compatible with other transgene control systems and could be assembled into higher-order control networks.
the benzoate-/vanillate-responsive device was compatible with other transgene control systems and could be assembled into higher-order control networks
The benzoate-/vanillate-responsive device was compatible with other transgene control systems and could be assembled into higher-order control networks.
the benzoate-/vanillate-responsive device was compatible with other transgene control systems and could be assembled into higher-order control networks
The authors designed mammalian gene expression systems that can be induced and repressed by the food additives benzoate and vanillate.
we have designed different mammalian gene expression systems that could be induced and repressed by the food additives benzoate and vanillate
The authors designed mammalian gene expression systems that can be induced and repressed by the food additives benzoate and vanillate.
we have designed different mammalian gene expression systems that could be induced and repressed by the food additives benzoate and vanillate
The authors designed mammalian gene expression systems that can be induced and repressed by the food additives benzoate and vanillate.
we have designed different mammalian gene expression systems that could be induced and repressed by the food additives benzoate and vanillate
The authors designed mammalian gene expression systems that can be induced and repressed by the food additives benzoate and vanillate.
we have designed different mammalian gene expression systems that could be induced and repressed by the food additives benzoate and vanillate
The authors designed mammalian gene expression systems that can be induced and repressed by the food additives benzoate and vanillate.
we have designed different mammalian gene expression systems that could be induced and repressed by the food additives benzoate and vanillate
The authors designed mammalian gene expression systems that can be induced and repressed by the food additives benzoate and vanillate.
we have designed different mammalian gene expression systems that could be induced and repressed by the food additives benzoate and vanillate
The authors designed mammalian gene expression systems that can be induced and repressed by the food additives benzoate and vanillate.
we have designed different mammalian gene expression systems that could be induced and repressed by the food additives benzoate and vanillate
In mice implanted with designer cells expressing SEAP under gene-switch control, blood SEAP levels directly correlated with a benzoate-enriched drinking program.
When implanting designer cells engineered for gene switch-driven expression of the human placental secreted alkaline phosphatase (SEAP) into mice, blood SEAP levels of treated animals directly correlated with a benzoate-enriched drinking programme.
In mice implanted with designer cells expressing SEAP under gene-switch control, blood SEAP levels directly correlated with a benzoate-enriched drinking program.
When implanting designer cells engineered for gene switch-driven expression of the human placental secreted alkaline phosphatase (SEAP) into mice, blood SEAP levels of treated animals directly correlated with a benzoate-enriched drinking programme.
In mice implanted with designer cells expressing SEAP under gene-switch control, blood SEAP levels directly correlated with a benzoate-enriched drinking program.
When implanting designer cells engineered for gene switch-driven expression of the human placental secreted alkaline phosphatase (SEAP) into mice, blood SEAP levels of treated animals directly correlated with a benzoate-enriched drinking programme.
In mice implanted with designer cells expressing SEAP under gene-switch control, blood SEAP levels directly correlated with a benzoate-enriched drinking program.
When implanting designer cells engineered for gene switch-driven expression of the human placental secreted alkaline phosphatase (SEAP) into mice, blood SEAP levels of treated animals directly correlated with a benzoate-enriched drinking programme.
In mice implanted with designer cells expressing SEAP under gene-switch control, blood SEAP levels directly correlated with a benzoate-enriched drinking program.
When implanting designer cells engineered for gene switch-driven expression of the human placental secreted alkaline phosphatase (SEAP) into mice, blood SEAP levels of treated animals directly correlated with a benzoate-enriched drinking programme.
In mice implanted with designer cells expressing SEAP under gene-switch control, blood SEAP levels directly correlated with a benzoate-enriched drinking program.
When implanting designer cells engineered for gene switch-driven expression of the human placental secreted alkaline phosphatase (SEAP) into mice, blood SEAP levels of treated animals directly correlated with a benzoate-enriched drinking programme.
In mice implanted with designer cells expressing SEAP under gene-switch control, blood SEAP levels directly correlated with a benzoate-enriched drinking program.
When implanting designer cells engineered for gene switch-driven expression of the human placental secreted alkaline phosphatase (SEAP) into mice, blood SEAP levels of treated animals directly correlated with a benzoate-enriched drinking programme.
Higher-order control networks assembled from the benzoate-/vanillate-responsive device provided expression dynamics reminiscent of a lap-timing stopwatch.
could be assembled into higher-order control networks providing expression dynamics reminiscent of a lap-timing stopwatch
Higher-order control networks assembled from the benzoate-/vanillate-responsive device provided expression dynamics reminiscent of a lap-timing stopwatch.
could be assembled into higher-order control networks providing expression dynamics reminiscent of a lap-timing stopwatch
Higher-order control networks assembled from the benzoate-/vanillate-responsive device provided expression dynamics reminiscent of a lap-timing stopwatch.
could be assembled into higher-order control networks providing expression dynamics reminiscent of a lap-timing stopwatch
Higher-order control networks assembled from the benzoate-/vanillate-responsive device provided expression dynamics reminiscent of a lap-timing stopwatch.
could be assembled into higher-order control networks providing expression dynamics reminiscent of a lap-timing stopwatch
Higher-order control networks assembled from the benzoate-/vanillate-responsive device provided expression dynamics reminiscent of a lap-timing stopwatch.
could be assembled into higher-order control networks providing expression dynamics reminiscent of a lap-timing stopwatch
Higher-order control networks assembled from the benzoate-/vanillate-responsive device provided expression dynamics reminiscent of a lap-timing stopwatch.
could be assembled into higher-order control networks providing expression dynamics reminiscent of a lap-timing stopwatch
Higher-order control networks assembled from the benzoate-/vanillate-responsive device provided expression dynamics reminiscent of a lap-timing stopwatch.
could be assembled into higher-order control networks providing expression dynamics reminiscent of a lap-timing stopwatch
Approval Evidence
1 source1 linked approval claimfirst-pass slug designer-cells-engineered-for-gene-switch-driven-seap-expression
designer cells engineered for gene switch-driven expression of the human placental secreted alkaline phosphatase (SEAP)
Source:
in vivo responsesupports
In mice implanted with designer cells expressing SEAP under gene-switch control, blood SEAP levels directly correlated with a benzoate-enriched drinking program.
When implanting designer cells engineered for gene switch-driven expression of the human placental secreted alkaline phosphatase (SEAP) into mice, blood SEAP levels of treated animals directly correlated with a benzoate-enriched drinking programme.
Source:
Comparisons
Source-backed strengths
The source reports that the gene expression system can be both induced and repressed by the food additives benzoate and vanillate. In implanted mice, blood SEAP levels directly correlated with a benzoate-enriched drinking program, providing in vivo functional validation. The device was also reported to be compatible with other transgene control systems and suitable for assembly into higher-order control networks.
designer cells engineered for gene switch-driven SEAP expression and cell-specific receptor subtype gene deletion mouse models address a similar problem space because they share recombination.
Shared frame: same top-level item type; shared target processes: recombination
Strengths here: looks easier to implement in practice.
Compared with eNpHR
designer cells engineered for gene switch-driven SEAP expression and eNpHR address a similar problem space because they share recombination.
Shared frame: same top-level item type; shared target processes: recombination
Strengths here: looks easier to implement in practice; may avoid an exogenous cofactor requirement.
Compared with katA::mCherry replacement construct
designer cells engineered for gene switch-driven SEAP expression and katA::mCherry replacement construct address a similar problem space because they share recombination.
Shared frame: same top-level item type; shared target processes: recombination
Ranked Citations
- 1.