Source 1primary paper2023iScience
Toolkit/Diya
Diya
Also known as: universal light illumination platform
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Diya is a universal light illumination platform for multiwell plate cultures that is compatible with a wide range of cell culture plates and dishes. It provides programmable optical induction and has been used to drive light-controlled operation of an in vivo biomolecular feedback circuit.
Usefulness & Problems
Why this is useful
Diya is useful as a general-purpose illumination harness for optically controlling cultured cells in standard multiwell and dish-based formats. The available evidence indicates that it supports light induction while maintaining viable culture conditions in bacteria, yeast, and human cell lines.
Source:
Finally, we demonstrate an optogenetic strategy for in vivo biomolecular controller operation. With a custom-designed antithetic integral feedback circuit, we exhibit robust perfect adaptation and light-controlled set-point variation using Diya.
Problem solved
Diya addresses the practical need for a single illumination platform that can interface with diverse cell culture vessels for programmable light induction experiments. It also supports implementation of light-controlled biomolecular control systems, including set-point variation in an antithetic integral feedback circuit.
Source:
Finally, we demonstrate an optogenetic strategy for in vivo biomolecular controller operation. With a custom-designed antithetic integral feedback circuit, we exhibit robust perfect adaptation and light-controlled set-point variation using Diya.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A delivery strategy grouped with the mechanism branch because it determines how a system is instantiated and deployed in context.
Techniques
Computational DesignTarget processes
No target processes tagged yet.
Input: Light
Implementation Constraints
The current summary states that Diya uses a custom-designed graphical user interface for programmable light induction. Beyond compatibility with multiple culture vessel formats and use in bacteria, yeast, and human cell lines, the supplied evidence does not specify construct design, illumination hardware, or operating parameters.
The supplied evidence does not provide quantitative optical specifications such as wavelength range, irradiance, spatial uniformity, or temporal resolution. It also does not describe hardware architecture, throughput limits, or independent replication beyond the cited study.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Using Diya with a custom-designed antithetic integral feedback circuit, the authors demonstrated in vivo biomolecular controller operation with robust perfect adaptation and light-controlled set-point variation.
Finally, we demonstrate an optogenetic strategy for in vivo biomolecular controller operation. With a custom-designed antithetic integral feedback circuit, we exhibit robust perfect adaptation and light-controlled set-point variation using Diya.
Using Diya with a custom-designed antithetic integral feedback circuit, the authors demonstrated in vivo biomolecular controller operation with robust perfect adaptation and light-controlled set-point variation.
Finally, we demonstrate an optogenetic strategy for in vivo biomolecular controller operation. With a custom-designed antithetic integral feedback circuit, we exhibit robust perfect adaptation and light-controlled set-point variation using Diya.
Using Diya with a custom-designed antithetic integral feedback circuit, the authors demonstrated in vivo biomolecular controller operation with robust perfect adaptation and light-controlled set-point variation.
Finally, we demonstrate an optogenetic strategy for in vivo biomolecular controller operation. With a custom-designed antithetic integral feedback circuit, we exhibit robust perfect adaptation and light-controlled set-point variation using Diya.
Using Diya with a custom-designed antithetic integral feedback circuit, the authors demonstrated in vivo biomolecular controller operation with robust perfect adaptation and light-controlled set-point variation.
Finally, we demonstrate an optogenetic strategy for in vivo biomolecular controller operation. With a custom-designed antithetic integral feedback circuit, we exhibit robust perfect adaptation and light-controlled set-point variation using Diya.
Using Diya with a custom-designed antithetic integral feedback circuit, the authors demonstrated in vivo biomolecular controller operation with robust perfect adaptation and light-controlled set-point variation.
Finally, we demonstrate an optogenetic strategy for in vivo biomolecular controller operation. With a custom-designed antithetic integral feedback circuit, we exhibit robust perfect adaptation and light-controlled set-point variation using Diya.
Using Diya with a custom-designed antithetic integral feedback circuit, the authors demonstrated in vivo biomolecular controller operation with robust perfect adaptation and light-controlled set-point variation.
Finally, we demonstrate an optogenetic strategy for in vivo biomolecular controller operation. With a custom-designed antithetic integral feedback circuit, we exhibit robust perfect adaptation and light-controlled set-point variation using Diya.
Using Diya with a custom-designed antithetic integral feedback circuit, the authors demonstrated in vivo biomolecular controller operation with robust perfect adaptation and light-controlled set-point variation.
Finally, we demonstrate an optogenetic strategy for in vivo biomolecular controller operation. With a custom-designed antithetic integral feedback circuit, we exhibit robust perfect adaptation and light-controlled set-point variation using Diya.
Diya maintains viable conditions for cell cultures undergoing light induction across bacteria, yeast, and human cell lines.
Through extensive characterization experiments with multiple optogenetic tools in diverse model organisms (bacteria, yeast, and human cell lines), we show that Diya maintains viable conditions for cell cultures undergoing light induction.
Diya maintains viable conditions for cell cultures undergoing light induction across bacteria, yeast, and human cell lines.
Through extensive characterization experiments with multiple optogenetic tools in diverse model organisms (bacteria, yeast, and human cell lines), we show that Diya maintains viable conditions for cell cultures undergoing light induction.
Diya maintains viable conditions for cell cultures undergoing light induction across bacteria, yeast, and human cell lines.
Through extensive characterization experiments with multiple optogenetic tools in diverse model organisms (bacteria, yeast, and human cell lines), we show that Diya maintains viable conditions for cell cultures undergoing light induction.
Diya maintains viable conditions for cell cultures undergoing light induction across bacteria, yeast, and human cell lines.
Through extensive characterization experiments with multiple optogenetic tools in diverse model organisms (bacteria, yeast, and human cell lines), we show that Diya maintains viable conditions for cell cultures undergoing light induction.
Diya maintains viable conditions for cell cultures undergoing light induction across bacteria, yeast, and human cell lines.
Through extensive characterization experiments with multiple optogenetic tools in diverse model organisms (bacteria, yeast, and human cell lines), we show that Diya maintains viable conditions for cell cultures undergoing light induction.
Diya maintains viable conditions for cell cultures undergoing light induction across bacteria, yeast, and human cell lines.
Through extensive characterization experiments with multiple optogenetic tools in diverse model organisms (bacteria, yeast, and human cell lines), we show that Diya maintains viable conditions for cell cultures undergoing light induction.
Diya maintains viable conditions for cell cultures undergoing light induction across bacteria, yeast, and human cell lines.
Through extensive characterization experiments with multiple optogenetic tools in diverse model organisms (bacteria, yeast, and human cell lines), we show that Diya maintains viable conditions for cell cultures undergoing light induction.
Diya is a universal light illumination platform compatible with a wide range of cell culture plates and dishes.
Here, we present a universal light illumination platform - Diya, compatible with a wide range of cell culture plates and dishes.
Diya is a universal light illumination platform compatible with a wide range of cell culture plates and dishes.
Here, we present a universal light illumination platform - Diya, compatible with a wide range of cell culture plates and dishes.
Diya is a universal light illumination platform compatible with a wide range of cell culture plates and dishes.
Here, we present a universal light illumination platform - Diya, compatible with a wide range of cell culture plates and dishes.
Diya is a universal light illumination platform compatible with a wide range of cell culture plates and dishes.
Here, we present a universal light illumination platform - Diya, compatible with a wide range of cell culture plates and dishes.
Diya is a universal light illumination platform compatible with a wide range of cell culture plates and dishes.
Here, we present a universal light illumination platform - Diya, compatible with a wide range of cell culture plates and dishes.
Diya is a universal light illumination platform compatible with a wide range of cell culture plates and dishes.
Here, we present a universal light illumination platform - Diya, compatible with a wide range of cell culture plates and dishes.
Diya is a universal light illumination platform compatible with a wide range of cell culture plates and dishes.
Here, we present a universal light illumination platform - Diya, compatible with a wide range of cell culture plates and dishes.
Diya offers programmable light induction through a custom-designed graphical user interface.
It offers light induction programming via a user-friendly custom-designed GUI.
Diya offers programmable light induction through a custom-designed graphical user interface.
It offers light induction programming via a user-friendly custom-designed GUI.
Diya offers programmable light induction through a custom-designed graphical user interface.
It offers light induction programming via a user-friendly custom-designed GUI.
Diya offers programmable light induction through a custom-designed graphical user interface.
It offers light induction programming via a user-friendly custom-designed GUI.
Diya offers programmable light induction through a custom-designed graphical user interface.
It offers light induction programming via a user-friendly custom-designed GUI.
Diya offers programmable light induction through a custom-designed graphical user interface.
It offers light induction programming via a user-friendly custom-designed GUI.
Diya offers programmable light induction through a custom-designed graphical user interface.
It offers light induction programming via a user-friendly custom-designed GUI.
Diya includes features for active thermal management, homogeneous illumination, and minimal light bleedthrough.
Diya hosts specially designed features ensuring active thermal management, homogeneous illumination, and minimal light bleedthrough.
Diya includes features for active thermal management, homogeneous illumination, and minimal light bleedthrough.
Diya hosts specially designed features ensuring active thermal management, homogeneous illumination, and minimal light bleedthrough.
Diya includes features for active thermal management, homogeneous illumination, and minimal light bleedthrough.
Diya hosts specially designed features ensuring active thermal management, homogeneous illumination, and minimal light bleedthrough.
Diya includes features for active thermal management, homogeneous illumination, and minimal light bleedthrough.
Diya hosts specially designed features ensuring active thermal management, homogeneous illumination, and minimal light bleedthrough.
Diya includes features for active thermal management, homogeneous illumination, and minimal light bleedthrough.
Diya hosts specially designed features ensuring active thermal management, homogeneous illumination, and minimal light bleedthrough.
Diya includes features for active thermal management, homogeneous illumination, and minimal light bleedthrough.
Diya hosts specially designed features ensuring active thermal management, homogeneous illumination, and minimal light bleedthrough.
Diya includes features for active thermal management, homogeneous illumination, and minimal light bleedthrough.
Diya hosts specially designed features ensuring active thermal management, homogeneous illumination, and minimal light bleedthrough.
Approval Evidence
1 source5 linked approval claimsfirst-pass slug diya
Here, we present a universal light illumination platform - Diya, compatible with a wide range of cell culture plates and dishes.
Source:
application demosupports
Using Diya with a custom-designed antithetic integral feedback circuit, the authors demonstrated in vivo biomolecular controller operation with robust perfect adaptation and light-controlled set-point variation.
Finally, we demonstrate an optogenetic strategy for in vivo biomolecular controller operation. With a custom-designed antithetic integral feedback circuit, we exhibit robust perfect adaptation and light-controlled set-point variation using Diya.
Source:
culture viability supportsupports
Diya maintains viable conditions for cell cultures undergoing light induction across bacteria, yeast, and human cell lines.
Through extensive characterization experiments with multiple optogenetic tools in diverse model organisms (bacteria, yeast, and human cell lines), we show that Diya maintains viable conditions for cell cultures undergoing light induction.
Source:
platform compatibilitysupports
Diya is a universal light illumination platform compatible with a wide range of cell culture plates and dishes.
Here, we present a universal light illumination platform - Diya, compatible with a wide range of cell culture plates and dishes.
Source:
platform control interfacesupports
Diya offers programmable light induction through a custom-designed graphical user interface.
It offers light induction programming via a user-friendly custom-designed GUI.
Source:
platform featuresupports
Diya includes features for active thermal management, homogeneous illumination, and minimal light bleedthrough.
Diya hosts specially designed features ensuring active thermal management, homogeneous illumination, and minimal light bleedthrough.
Source:
Comparisons
Source-backed strengths
A reported strength is broad physical compatibility with a wide range of cell culture plates and dishes. It was also demonstrated in an application involving in vivo biomolecular controller operation with robust perfect adaptation and light-controlled set-point variation, and it reportedly maintained viable conditions across bacterial, yeast, and human cell cultures.
Ranked Citations
- 1.