Source 1review2015Biotechnology Journal
Toolkit/DNA synthesis
DNA synthesis
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
DNA synthesis is presented as an engineering method that supports the development of new dynamic metabolic engineering systems. In the cited review, advances in DNA synthesis are identified as a factor that will continue to drive innovation in responsive cell factory design.
Usefulness & Problems
Why this is useful
The cited evidence supports DNA synthesis as an enabling method for building new dynamic regulation systems in metabolic engineering. This is useful in the context of responsive cell factories, where dynamic control strategies are used to rebalance metabolic fluxes under changing cellular or fermentation conditions.
Problem solved
Within the supplied review, DNA synthesis contributes to the broader challenge of constructing dynamic control systems, which are more complex to implement than static control. These dynamic systems are described as helping manage trade-offs between growth and production and avoid accumulation of undesired intermediates.
Problem links
Need better screening or enrichment leverage
DerivedDNA synthesis is presented as an engineering method that supports the development of new dynamic metabolic engineering systems. In the cited review, advances in DNA synthesis are identified as a driver of continued innovation in responsive cell factory design.
Need conditional recombination or state switching
DerivedDNA synthesis is presented as an engineering method that supports the development of new dynamic metabolic engineering systems. In the cited review, advances in DNA synthesis are identified as a driver of continued innovation in responsive cell factory design.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete method used to build, optimize, or evolve an engineered system.
Mechanisms
No mechanism tags yet.
Target processes
editingrecombinationselectionImplementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationmodality: build technologyoperating role: builder
The review-level evidence only indicates that DNA synthesis supports innovation in dynamic system development. No practical details are provided on construct design, assembly format, host organisms, delivery methods, or integration with recombination or selection workflows.
The supplied evidence does not describe a specific DNA synthesis platform, workflow, sequence scale, fidelity, or organismal validation. It also does not provide direct experimental examples showing how DNA synthesis alone improves dynamic system performance.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Source 2primary paper2025MED
Ranked Claims
DNA synthesis, programmable gene circuits, and CRISPR/Cas-based genome editing are fundamental technologies enabling advances in plant synthetic biology.
This review focuses on the fundamental technologies that have enabled such advances, which include DNA synthesis, programmable gene circuits, and CRISPR/Cas-based genome editing.
Advances in high-throughput screening, screening techniques, synthetic biology, and DNA synthesis support development and innovation of new dynamic systems.
explores how advances in high-throughput screening and synthetic biology can support development of new dynamic systems... advances in screening techniques and DNA synthesis will continue to drive innovation in this field
Advances in high-throughput screening, screening techniques, synthetic biology, and DNA synthesis support development and innovation of new dynamic systems.
explores how advances in high-throughput screening and synthetic biology can support development of new dynamic systems... advances in screening techniques and DNA synthesis will continue to drive innovation in this field
Advances in high-throughput screening, screening techniques, synthetic biology, and DNA synthesis support development and innovation of new dynamic systems.
explores how advances in high-throughput screening and synthetic biology can support development of new dynamic systems... advances in screening techniques and DNA synthesis will continue to drive innovation in this field
Advances in high-throughput screening, screening techniques, synthetic biology, and DNA synthesis support development and innovation of new dynamic systems.
explores how advances in high-throughput screening and synthetic biology can support development of new dynamic systems... advances in screening techniques and DNA synthesis will continue to drive innovation in this field
Advances in high-throughput screening, screening techniques, synthetic biology, and DNA synthesis support development and innovation of new dynamic systems.
explores how advances in high-throughput screening and synthetic biology can support development of new dynamic systems... advances in screening techniques and DNA synthesis will continue to drive innovation in this field
Advances in high-throughput screening, screening techniques, synthetic biology, and DNA synthesis support development and innovation of new dynamic systems.
explores how advances in high-throughput screening and synthetic biology can support development of new dynamic systems... advances in screening techniques and DNA synthesis will continue to drive innovation in this field
Advances in high-throughput screening, screening techniques, synthetic biology, and DNA synthesis support development and innovation of new dynamic systems.
explores how advances in high-throughput screening and synthetic biology can support development of new dynamic systems... advances in screening techniques and DNA synthesis will continue to drive innovation in this field
Advances in high-throughput screening, screening techniques, synthetic biology, and DNA synthesis support development and innovation of new dynamic systems.
explores how advances in high-throughput screening and synthetic biology can support development of new dynamic systems... advances in screening techniques and DNA synthesis will continue to drive innovation in this field
Advances in high-throughput screening, screening techniques, synthetic biology, and DNA synthesis support development and innovation of new dynamic systems.
explores how advances in high-throughput screening and synthetic biology can support development of new dynamic systems... advances in screening techniques and DNA synthesis will continue to drive innovation in this field
Advances in high-throughput screening, screening techniques, synthetic biology, and DNA synthesis support development and innovation of new dynamic systems.
explores how advances in high-throughput screening and synthetic biology can support development of new dynamic systems... advances in screening techniques and DNA synthesis will continue to drive innovation in this field
Advances in high-throughput screening, screening techniques, synthetic biology, and DNA synthesis support development and innovation of new dynamic systems.
explores how advances in high-throughput screening and synthetic biology can support development of new dynamic systems... advances in screening techniques and DNA synthesis will continue to drive innovation in this field
Advances in high-throughput screening, screening techniques, synthetic biology, and DNA synthesis support development and innovation of new dynamic systems.
explores how advances in high-throughput screening and synthetic biology can support development of new dynamic systems... advances in screening techniques and DNA synthesis will continue to drive innovation in this field
Advances in high-throughput screening, screening techniques, synthetic biology, and DNA synthesis support development and innovation of new dynamic systems.
explores how advances in high-throughput screening and synthetic biology can support development of new dynamic systems... advances in screening techniques and DNA synthesis will continue to drive innovation in this field
Advances in high-throughput screening, screening techniques, synthetic biology, and DNA synthesis support development and innovation of new dynamic systems.
explores how advances in high-throughput screening and synthetic biology can support development of new dynamic systems... advances in screening techniques and DNA synthesis will continue to drive innovation in this field
Advances in high-throughput screening, screening techniques, synthetic biology, and DNA synthesis support development and innovation of new dynamic systems.
explores how advances in high-throughput screening and synthetic biology can support development of new dynamic systems... advances in screening techniques and DNA synthesis will continue to drive innovation in this field
Advances in high-throughput screening, screening techniques, synthetic biology, and DNA synthesis support development and innovation of new dynamic systems.
explores how advances in high-throughput screening and synthetic biology can support development of new dynamic systems... advances in screening techniques and DNA synthesis will continue to drive innovation in this field
Advances in high-throughput screening, screening techniques, synthetic biology, and DNA synthesis support development and innovation of new dynamic systems.
explores how advances in high-throughput screening and synthetic biology can support development of new dynamic systems... advances in screening techniques and DNA synthesis will continue to drive innovation in this field
Advances in high-throughput screening, screening techniques, synthetic biology, and DNA synthesis support development and innovation of new dynamic systems.
explores how advances in high-throughput screening and synthetic biology can support development of new dynamic systems... advances in screening techniques and DNA synthesis will continue to drive innovation in this field
Advances in high-throughput screening, screening techniques, synthetic biology, and DNA synthesis support development and innovation of new dynamic systems.
explores how advances in high-throughput screening and synthetic biology can support development of new dynamic systems... advances in screening techniques and DNA synthesis will continue to drive innovation in this field
Advances in high-throughput screening, screening techniques, synthetic biology, and DNA synthesis support development and innovation of new dynamic systems.
explores how advances in high-throughput screening and synthetic biology can support development of new dynamic systems... advances in screening techniques and DNA synthesis will continue to drive innovation in this field
Advances in high-throughput screening, screening techniques, synthetic biology, and DNA synthesis support development and innovation of new dynamic systems.
explores how advances in high-throughput screening and synthetic biology can support development of new dynamic systems... advances in screening techniques and DNA synthesis will continue to drive innovation in this field
Advances in high-throughput screening, screening techniques, synthetic biology, and DNA synthesis support development and innovation of new dynamic systems.
explores how advances in high-throughput screening and synthetic biology can support development of new dynamic systems... advances in screening techniques and DNA synthesis will continue to drive innovation in this field
Advances in high-throughput screening, screening techniques, synthetic biology, and DNA synthesis support development and innovation of new dynamic systems.
explores how advances in high-throughput screening and synthetic biology can support development of new dynamic systems... advances in screening techniques and DNA synthesis will continue to drive innovation in this field
Advances in high-throughput screening, screening techniques, synthetic biology, and DNA synthesis support development and innovation of new dynamic systems.
explores how advances in high-throughput screening and synthetic biology can support development of new dynamic systems... advances in screening techniques and DNA synthesis will continue to drive innovation in this field
Advances in high-throughput screening, screening techniques, synthetic biology, and DNA synthesis support development and innovation of new dynamic systems.
explores how advances in high-throughput screening and synthetic biology can support development of new dynamic systems... advances in screening techniques and DNA synthesis will continue to drive innovation in this field
Advances in high-throughput screening, screening techniques, synthetic biology, and DNA synthesis support development and innovation of new dynamic systems.
explores how advances in high-throughput screening and synthetic biology can support development of new dynamic systems... advances in screening techniques and DNA synthesis will continue to drive innovation in this field
Advances in high-throughput screening, screening techniques, synthetic biology, and DNA synthesis support development and innovation of new dynamic systems.
explores how advances in high-throughput screening and synthetic biology can support development of new dynamic systems... advances in screening techniques and DNA synthesis will continue to drive innovation in this field
Dynamic gene expression profiles can help manage trade-offs between growth and production and avoid buildup of undesired intermediates.
Dynamic gene expression profiles allow trade-offs between growth and production to be better managed and can help avoid build-up of undesired intermediates.
Dynamic gene expression profiles can help manage trade-offs between growth and production and avoid buildup of undesired intermediates.
Dynamic gene expression profiles allow trade-offs between growth and production to be better managed and can help avoid build-up of undesired intermediates.
Dynamic gene expression profiles can help manage trade-offs between growth and production and avoid buildup of undesired intermediates.
Dynamic gene expression profiles allow trade-offs between growth and production to be better managed and can help avoid build-up of undesired intermediates.
Dynamic gene expression profiles can help manage trade-offs between growth and production and avoid buildup of undesired intermediates.
Dynamic gene expression profiles allow trade-offs between growth and production to be better managed and can help avoid build-up of undesired intermediates.
Dynamic gene expression profiles can help manage trade-offs between growth and production and avoid buildup of undesired intermediates.
Dynamic gene expression profiles allow trade-offs between growth and production to be better managed and can help avoid build-up of undesired intermediates.
Dynamic gene expression profiles can help manage trade-offs between growth and production and avoid buildup of undesired intermediates.
Dynamic gene expression profiles allow trade-offs between growth and production to be better managed and can help avoid build-up of undesired intermediates.
Dynamic gene expression profiles can help manage trade-offs between growth and production and avoid buildup of undesired intermediates.
Dynamic gene expression profiles allow trade-offs between growth and production to be better managed and can help avoid build-up of undesired intermediates.
Dynamic gene expression profiles can help manage trade-offs between growth and production and avoid buildup of undesired intermediates.
Dynamic gene expression profiles allow trade-offs between growth and production to be better managed and can help avoid build-up of undesired intermediates.
Dynamic gene expression profiles can help manage trade-offs between growth and production and avoid buildup of undesired intermediates.
Dynamic gene expression profiles allow trade-offs between growth and production to be better managed and can help avoid build-up of undesired intermediates.
Dynamic gene expression profiles can help manage trade-offs between growth and production and avoid buildup of undesired intermediates.
Dynamic gene expression profiles allow trade-offs between growth and production to be better managed and can help avoid build-up of undesired intermediates.
Dynamic gene expression profiles can help manage trade-offs between growth and production and avoid buildup of undesired intermediates.
Dynamic gene expression profiles allow trade-offs between growth and production to be better managed and can help avoid build-up of undesired intermediates.
Dynamic gene expression profiles can help manage trade-offs between growth and production and avoid buildup of undesired intermediates.
Dynamic gene expression profiles allow trade-offs between growth and production to be better managed and can help avoid build-up of undesired intermediates.
Dynamic gene expression profiles can help manage trade-offs between growth and production and avoid buildup of undesired intermediates.
Dynamic gene expression profiles allow trade-offs between growth and production to be better managed and can help avoid build-up of undesired intermediates.
Dynamic gene expression profiles can help manage trade-offs between growth and production and avoid buildup of undesired intermediates.
Dynamic gene expression profiles allow trade-offs between growth and production to be better managed and can help avoid build-up of undesired intermediates.
Dynamic gene expression profiles can help manage trade-offs between growth and production and avoid buildup of undesired intermediates.
Dynamic gene expression profiles allow trade-offs between growth and production to be better managed and can help avoid build-up of undesired intermediates.
Dynamic gene expression profiles can help manage trade-offs between growth and production and avoid buildup of undesired intermediates.
Dynamic gene expression profiles allow trade-offs between growth and production to be better managed and can help avoid build-up of undesired intermediates.
Dynamic gene expression profiles can help manage trade-offs between growth and production and avoid buildup of undesired intermediates.
Dynamic gene expression profiles allow trade-offs between growth and production to be better managed and can help avoid build-up of undesired intermediates.
Dynamic gene expression profiles can help manage trade-offs between growth and production and avoid buildup of undesired intermediates.
Dynamic gene expression profiles allow trade-offs between growth and production to be better managed and can help avoid build-up of undesired intermediates.
Dynamic gene expression profiles can help manage trade-offs between growth and production and avoid buildup of undesired intermediates.
Dynamic gene expression profiles allow trade-offs between growth and production to be better managed and can help avoid build-up of undesired intermediates.
Dynamic gene expression profiles can help manage trade-offs between growth and production and avoid buildup of undesired intermediates.
Dynamic gene expression profiles allow trade-offs between growth and production to be better managed and can help avoid build-up of undesired intermediates.
Dynamic regulation strategies allow rebalancing of metabolic fluxes according to changing cellular or fermentation conditions.
a number of groups have developed strategies for dynamic regulation, which allows rebalancing of fluxes according to changing conditions in the cell or the fermentation medium
Dynamic regulation strategies allow rebalancing of metabolic fluxes according to changing cellular or fermentation conditions.
a number of groups have developed strategies for dynamic regulation, which allows rebalancing of fluxes according to changing conditions in the cell or the fermentation medium
Dynamic regulation strategies allow rebalancing of metabolic fluxes according to changing cellular or fermentation conditions.
a number of groups have developed strategies for dynamic regulation, which allows rebalancing of fluxes according to changing conditions in the cell or the fermentation medium
Dynamic regulation strategies allow rebalancing of metabolic fluxes according to changing cellular or fermentation conditions.
a number of groups have developed strategies for dynamic regulation, which allows rebalancing of fluxes according to changing conditions in the cell or the fermentation medium
Dynamic regulation strategies allow rebalancing of metabolic fluxes according to changing cellular or fermentation conditions.
a number of groups have developed strategies for dynamic regulation, which allows rebalancing of fluxes according to changing conditions in the cell or the fermentation medium
Dynamic regulation strategies allow rebalancing of metabolic fluxes according to changing cellular or fermentation conditions.
a number of groups have developed strategies for dynamic regulation, which allows rebalancing of fluxes according to changing conditions in the cell or the fermentation medium
Dynamic regulation strategies allow rebalancing of metabolic fluxes according to changing cellular or fermentation conditions.
a number of groups have developed strategies for dynamic regulation, which allows rebalancing of fluxes according to changing conditions in the cell or the fermentation medium
Dynamic regulation strategies allow rebalancing of metabolic fluxes according to changing cellular or fermentation conditions.
a number of groups have developed strategies for dynamic regulation, which allows rebalancing of fluxes according to changing conditions in the cell or the fermentation medium
Dynamic regulation strategies allow rebalancing of metabolic fluxes according to changing cellular or fermentation conditions.
a number of groups have developed strategies for dynamic regulation, which allows rebalancing of fluxes according to changing conditions in the cell or the fermentation medium
Dynamic regulation strategies allow rebalancing of metabolic fluxes according to changing cellular or fermentation conditions.
a number of groups have developed strategies for dynamic regulation, which allows rebalancing of fluxes according to changing conditions in the cell or the fermentation medium
Dynamic regulation strategies allow rebalancing of metabolic fluxes according to changing cellular or fermentation conditions.
a number of groups have developed strategies for dynamic regulation, which allows rebalancing of fluxes according to changing conditions in the cell or the fermentation medium
Dynamic regulation strategies allow rebalancing of metabolic fluxes according to changing cellular or fermentation conditions.
a number of groups have developed strategies for dynamic regulation, which allows rebalancing of fluxes according to changing conditions in the cell or the fermentation medium
Dynamic regulation strategies allow rebalancing of metabolic fluxes according to changing cellular or fermentation conditions.
a number of groups have developed strategies for dynamic regulation, which allows rebalancing of fluxes according to changing conditions in the cell or the fermentation medium
Dynamic regulation strategies allow rebalancing of metabolic fluxes according to changing cellular or fermentation conditions.
a number of groups have developed strategies for dynamic regulation, which allows rebalancing of fluxes according to changing conditions in the cell or the fermentation medium
Dynamic regulation strategies allow rebalancing of metabolic fluxes according to changing cellular or fermentation conditions.
a number of groups have developed strategies for dynamic regulation, which allows rebalancing of fluxes according to changing conditions in the cell or the fermentation medium
Dynamic regulation strategies allow rebalancing of metabolic fluxes according to changing cellular or fermentation conditions.
a number of groups have developed strategies for dynamic regulation, which allows rebalancing of fluxes according to changing conditions in the cell or the fermentation medium
Dynamic regulation strategies allow rebalancing of metabolic fluxes according to changing cellular or fermentation conditions.
a number of groups have developed strategies for dynamic regulation, which allows rebalancing of fluxes according to changing conditions in the cell or the fermentation medium
Dynamic regulation strategies allow rebalancing of metabolic fluxes according to changing cellular or fermentation conditions.
a number of groups have developed strategies for dynamic regulation, which allows rebalancing of fluxes according to changing conditions in the cell or the fermentation medium
Dynamic regulation strategies allow rebalancing of metabolic fluxes according to changing cellular or fermentation conditions.
a number of groups have developed strategies for dynamic regulation, which allows rebalancing of fluxes according to changing conditions in the cell or the fermentation medium
Dynamic regulation strategies allow rebalancing of metabolic fluxes according to changing cellular or fermentation conditions.
a number of groups have developed strategies for dynamic regulation, which allows rebalancing of fluxes according to changing conditions in the cell or the fermentation medium
Implementation of dynamic control is more complex than static control.
The implementation is more complex relative to static control
Implementation of dynamic control is more complex than static control.
The implementation is more complex relative to static control
Implementation of dynamic control is more complex than static control.
The implementation is more complex relative to static control
Implementation of dynamic control is more complex than static control.
The implementation is more complex relative to static control
Implementation of dynamic control is more complex than static control.
The implementation is more complex relative to static control
Implementation of dynamic control is more complex than static control.
The implementation is more complex relative to static control
Implementation of dynamic control is more complex than static control.
The implementation is more complex relative to static control
Implementation of dynamic control is more complex than static control.
The implementation is more complex relative to static control
Implementation of dynamic control is more complex than static control.
The implementation is more complex relative to static control
Implementation of dynamic control is more complex than static control.
The implementation is more complex relative to static control
Implementation of dynamic control is more complex than static control.
The implementation is more complex relative to static control
Implementation of dynamic control is more complex than static control.
The implementation is more complex relative to static control
Implementation of dynamic control is more complex than static control.
The implementation is more complex relative to static control
Implementation of dynamic control is more complex than static control.
The implementation is more complex relative to static control
Implementation of dynamic control is more complex than static control.
The implementation is more complex relative to static control
Implementation of dynamic control is more complex than static control.
The implementation is more complex relative to static control
Implementation of dynamic control is more complex than static control.
The implementation is more complex relative to static control
Implementation of dynamic control is more complex than static control.
The implementation is more complex relative to static control
Implementation of dynamic control is more complex than static control.
The implementation is more complex relative to static control
Implementation of dynamic control is more complex than static control.
The implementation is more complex relative to static control
Approval Evidence
2 sources2 linked approval claimsfirst-pass slug dna-synthesis
This review focuses on the fundamental technologies that have enabled such advances, which include DNA synthesis, programmable gene circuits, and CRISPR/Cas-based genome editing.
Source:
advances in screening techniques and DNA synthesis will continue to drive innovation in this field
Source:
enabling technologysupports
DNA synthesis, programmable gene circuits, and CRISPR/Cas-based genome editing are fundamental technologies enabling advances in plant synthetic biology.
This review focuses on the fundamental technologies that have enabled such advances, which include DNA synthesis, programmable gene circuits, and CRISPR/Cas-based genome editing.
Source:
review summarysupports
Advances in high-throughput screening, screening techniques, synthetic biology, and DNA synthesis support development and innovation of new dynamic systems.
explores how advances in high-throughput screening and synthetic biology can support development of new dynamic systems... advances in screening techniques and DNA synthesis will continue to drive innovation in this field
Source:
Comparisons
Source-backed strengths
A stated strength is its role in supporting continued innovation of dynamic metabolic engineering when combined with advances in screening techniques. The evidence links DNA synthesis to the development of responsive cell factory strategies, but does not provide direct performance metrics or comparative benchmarks.
Compared with CRISPR/Cas9
DNA synthesis and CRISPR/Cas9 address a similar problem space because they share recombination, selection.
Shared frame: same top-level item type; shared target processes: recombination, selection
Strengths here: looks easier to implement in practice.
Compared with genetic screens in Arabidopsis thaliana
DNA synthesis and genetic screens in Arabidopsis thaliana address a similar problem space because they share recombination, selection.
Shared frame: same top-level item type; shared target processes: recombination, selection
Strengths here: looks easier to implement in practice.
Compared with multiplexed engineering
DNA synthesis and multiplexed engineering address a similar problem space because they share recombination, selection.
Shared frame: same top-level item type; shared target processes: recombination, selection
Ranked Citations
- 1.
- 2.