Source 1review2018International Journal of Genomics
Toolkit/domain fusion
domain fusion
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Domain fusion is a protein engineering method in which protein domains are fused or split to improve existing protein functions or create novel functions. In the supplied evidence, it is described as a general strategy for expanding CRISPR-Cas9 applications.
Usefulness & Problems
Why this is useful
This method is useful because it provides a general route to alter or extend protein function, including in CRISPR-Cas9 systems. The cited context links such engineering strategies to applications spanning gene therapy, gene regulation, epigenome modification, and chromosome imaging.
Source:
CRISPR-Cas9 has been used in a wide variety of applications ranging from basic science to the clinic, such as gene therapy, gene regulation, modifying epigenomes, and imaging chromosomes.
Problem solved
In the supplied evidence, domain fusion is relevant to addressing limitations that restrict broader CRISPR-Cas9 use. These limitations include aberrant off-target activity, strict protospacer-adjacent motif dependence, and the large size of Cas9 that complicates delivery.
Source:
CRISPR-Cas9 has been used in a wide variety of applications ranging from basic science to the clinic, such as gene therapy, gene regulation, modifying epigenomes, and imaging chromosomes.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete method used to build, optimize, or evolve an engineered system.
Target processes
No target processes tagged yet.
Implementation Constraints
The evidence only states that protein functions can be improved or newly developed through domain fusion or splitting. It does not provide construct architecture, linker design, host system, delivery modality, or assay details.
The supplied evidence does not provide a specific fused construct, quantitative performance data, or direct validation outcomes for any one domain-fusion design. It also does not specify which domain combinations solve off-targeting, PAM restriction, or delivery constraints.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
CRISPR-Cas9 has been used across applications including gene therapy, gene regulation, epigenome modification, and chromosome imaging.
CRISPR-Cas9 has been used in a wide variety of applications ranging from basic science to the clinic, such as gene therapy, gene regulation, modifying epigenomes, and imaging chromosomes.
These Cas9 limitations hinder the use of CRISPR for disease treatment and wider biotechnological applications.
These obstacles hinder the use of CRISPR for disease treatment and in wider biotechnological applications.
Cas9 can exhibit aberrant off-target activity.
(ii) aberrant off-target activity
Cas9 has a strict dependence on a protospacer-adjacent motif sequence.
some limitations have also been reported, for instance (i) a strict dependence on a protospacer-adjacent motif (PAM) sequence
Cas9 lacks sufficient modulation of protein binding and endonuclease activity for precise spatiotemporal control.
(iv) lack of modulation of protein binding and endonuclease activity, which is crucial for precise spatiotemporal control of gene expression or genome editing
The large size of Cas9 creates problems for CRISPR delivery.
(iii) the large size of Cas9 is problematic for CRISPR delivery
The review emphasizes domain fusion or splitting, rational design, and directed evolution as protein-engineering strategies for expanding SpCas9 versatility.
Here, recent protein-engineering approaches for expanding the versatility of the Streptococcus pyogenes Cas9 (SpCas9) is reviewed, with an emphasis on studies that improve or develop novel protein functions through domain fusion or splitting, rational design, and directed evolution.
The review emphasizes domain fusion or splitting, rational design, and directed evolution as protein-engineering strategies for expanding SpCas9 versatility.
Here, recent protein-engineering approaches for expanding the versatility of the Streptococcus pyogenes Cas9 (SpCas9) is reviewed, with an emphasis on studies that improve or develop novel protein functions through domain fusion or splitting, rational design, and directed evolution.
The review emphasizes domain fusion or splitting, rational design, and directed evolution as protein-engineering strategies for expanding SpCas9 versatility.
Here, recent protein-engineering approaches for expanding the versatility of the Streptococcus pyogenes Cas9 (SpCas9) is reviewed, with an emphasis on studies that improve or develop novel protein functions through domain fusion or splitting, rational design, and directed evolution.
The review emphasizes domain fusion or splitting, rational design, and directed evolution as protein-engineering strategies for expanding SpCas9 versatility.
Here, recent protein-engineering approaches for expanding the versatility of the Streptococcus pyogenes Cas9 (SpCas9) is reviewed, with an emphasis on studies that improve or develop novel protein functions through domain fusion or splitting, rational design, and directed evolution.
The review emphasizes domain fusion or splitting, rational design, and directed evolution as protein-engineering strategies for expanding SpCas9 versatility.
Here, recent protein-engineering approaches for expanding the versatility of the Streptococcus pyogenes Cas9 (SpCas9) is reviewed, with an emphasis on studies that improve or develop novel protein functions through domain fusion or splitting, rational design, and directed evolution.
The review emphasizes domain fusion or splitting, rational design, and directed evolution as protein-engineering strategies for expanding SpCas9 versatility.
Here, recent protein-engineering approaches for expanding the versatility of the Streptococcus pyogenes Cas9 (SpCas9) is reviewed, with an emphasis on studies that improve or develop novel protein functions through domain fusion or splitting, rational design, and directed evolution.
The review emphasizes domain fusion or splitting, rational design, and directed evolution as protein-engineering strategies for expanding SpCas9 versatility.
Here, recent protein-engineering approaches for expanding the versatility of the Streptococcus pyogenes Cas9 (SpCas9) is reviewed, with an emphasis on studies that improve or develop novel protein functions through domain fusion or splitting, rational design, and directed evolution.
Protein-engineering approaches are presented as solutions to overcome Cas9 limitations and generate more robust and efficient DNA manipulation tools.
Protein-engineering approaches offer solutions to overcome the limitations of Cas9 and generate robust and efficient tools for customized DNA manipulation.
Protein-engineering approaches are presented as solutions to overcome Cas9 limitations and generate more robust and efficient DNA manipulation tools.
Protein-engineering approaches offer solutions to overcome the limitations of Cas9 and generate robust and efficient tools for customized DNA manipulation.
Protein-engineering approaches are presented as solutions to overcome Cas9 limitations and generate more robust and efficient DNA manipulation tools.
Protein-engineering approaches offer solutions to overcome the limitations of Cas9 and generate robust and efficient tools for customized DNA manipulation.
Protein-engineering approaches are presented as solutions to overcome Cas9 limitations and generate more robust and efficient DNA manipulation tools.
Protein-engineering approaches offer solutions to overcome the limitations of Cas9 and generate robust and efficient tools for customized DNA manipulation.
Protein-engineering approaches are presented as solutions to overcome Cas9 limitations and generate more robust and efficient DNA manipulation tools.
Protein-engineering approaches offer solutions to overcome the limitations of Cas9 and generate robust and efficient tools for customized DNA manipulation.
Protein-engineering approaches are presented as solutions to overcome Cas9 limitations and generate more robust and efficient DNA manipulation tools.
Protein-engineering approaches offer solutions to overcome the limitations of Cas9 and generate robust and efficient tools for customized DNA manipulation.
Protein-engineering approaches are presented as solutions to overcome Cas9 limitations and generate more robust and efficient DNA manipulation tools.
Protein-engineering approaches offer solutions to overcome the limitations of Cas9 and generate robust and efficient tools for customized DNA manipulation.
Approval Evidence
1 source2 linked approval claimsfirst-pass slug domain-fusion
studies that improve or develop novel protein functions through domain fusion or splitting
Source:
review focussupports
The review emphasizes domain fusion or splitting, rational design, and directed evolution as protein-engineering strategies for expanding SpCas9 versatility.
Here, recent protein-engineering approaches for expanding the versatility of the Streptococcus pyogenes Cas9 (SpCas9) is reviewed, with an emphasis on studies that improve or develop novel protein functions through domain fusion or splitting, rational design, and directed evolution.
Source:
strategy overviewsupports
Protein-engineering approaches are presented as solutions to overcome Cas9 limitations and generate more robust and efficient DNA manipulation tools.
Protein-engineering approaches offer solutions to overcome the limitations of Cas9 and generate robust and efficient tools for customized DNA manipulation.
Source:
Comparisons
Source-backed strengths
The evidence supports domain fusion and domain splitting as flexible strategies for improving existing protein functions or generating novel ones. It is presented as broadly applicable within efforts to expand CRISPR-Cas9 functionality across multiple use contexts.
Ranked Citations
- 1.