Source 1primary paper2020
Toolkit/drug inducible lentiviral and transposon vectors
drug inducible lentiviral and transposon vectors
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Drug-inducible lentiviral and transposon vectors were used to deliver the PhyB-PIF light-inducible dimerization system together with the synPCB phycocyanobilin synthesis module. In the cited study, doxycycline treatment induced PCB synthesis and enabled PhyB-PIF light-inducible dimerization function.
Usefulness & Problems
Why this is useful
These vectors provide a delivery harness for stable introduction and inducible control of both an optogenetic dimerization module and its required chromophore biosynthesis pathway. This is useful when PhyB-PIF function must be coupled to regulated intracellular PCB production rather than constitutive expression.
Source:
These inducible lentiviral and transposon vectors were used to carry synPCB and PhyB-PIF. The abstract states that they enabled doxycycline-inducible PCB synthesis and PhyB-PIF LID activity.
Source:
stable delivery of synPCB and PhyB-PIF
Source:
doxycycline-inducible activation of PCB synthesis and the LID system
Problem solved
The system addresses the need to co-deliver and drug-inducibly control PhyB-PIF and synPCB modules so that PCB synthesis and phytochrome-based light responses can be turned on by doxycycline. The supplied evidence does not show that the vectors themselves improve PCB synthesis beyond enabling inducible module expression.
Source:
They provide a way to stably introduce and inducibly control both the chromophore synthesis module and the optogenetic dimerization system.
Source:
enables inducible deployment of the chromophore synthesis system and optogenetic switch
Problem links
enables inducible deployment of the chromophore synthesis system and optogenetic switch
LiteratureThey provide a way to stably introduce and inducibly control both the chromophore synthesis module and the optogenetic dimerization system.
Source:
They provide a way to stably introduce and inducibly control both the chromophore synthesis module and the optogenetic dimerization system.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A delivery strategy grouped with the mechanism branch because it determines how a system is instantiated and deployed in context.
Mechanisms
drug-inducible expressiondrug-inducible expressionHeterodimerizationHeterodimerizationHeterodimerizationlight-controlled association and dissociationlight-controlled association and dissociationTechniques
No technique tags yet.
Target processes
No target processes tagged yet.
Input: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknowncofactor dependency: requires exogenous cofactorencoding mode: externally suppliedimplementation constraint: context specific validationimplementation constraint: multi component delivery burdenimplementation constraint: payload burdenimplementation constraint: spectral hardware requirementinducible drug: doxycyclineoperating role: deliverysupports stable cell line: Trueswitch architecture: multi componentswitch architecture: recruitment
Implementation involved incorporating both PhyB-PIF and synPCB into drug-inducible lentiviral and transposon vectors. Operation required doxycycline treatment to induce PCB synthesis and the PhyB-PIF light-inducible dimerization system.
Evidence is limited to a single cited study and a brief statement that inducible delivery was achieved with lentiviral and transposon formats. The provided evidence does not report quantitative induction levels, cell-type scope, vector architecture, or comparative performance between the two delivery systems.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Observations
successMammalian Cell Lineapplication demo
Inferred from claim c3 during normalization. The authors established a stable cell line containing a genetically encoded PhyB-PIF light-inducible dimerization system. Derived from claim c3. Quoted text: successfully established a stable cell line containing a genetically encoded PhyB-PIF LID system
Source:
successMammalian Cell Lineapplication demo
Inferred from claim c3 during normalization. The authors established a stable cell line containing a genetically encoded PhyB-PIF light-inducible dimerization system. Derived from claim c3. Quoted text: successfully established a stable cell line containing a genetically encoded PhyB-PIF LID system
Source:
successMammalian Cell Lineapplication demo
Inferred from claim c3 during normalization. The authors established a stable cell line containing a genetically encoded PhyB-PIF light-inducible dimerization system. Derived from claim c3. Quoted text: successfully established a stable cell line containing a genetically encoded PhyB-PIF LID system
Source:
successMammalian Cell Lineapplication demo
Inferred from claim c3 during normalization. The authors established a stable cell line containing a genetically encoded PhyB-PIF light-inducible dimerization system. Derived from claim c3. Quoted text: successfully established a stable cell line containing a genetically encoded PhyB-PIF LID system
Source:
successMammalian Cell Lineapplication demo
Inferred from claim c3 during normalization. The authors established a stable cell line containing a genetically encoded PhyB-PIF light-inducible dimerization system. Derived from claim c3. Quoted text: successfully established a stable cell line containing a genetically encoded PhyB-PIF LID system
Source:
successMammalian Cell Lineapplication demo
Inferred from claim c3 during normalization. The authors established a stable cell line containing a genetically encoded PhyB-PIF light-inducible dimerization system. Derived from claim c3. Quoted text: successfully established a stable cell line containing a genetically encoded PhyB-PIF LID system
Source:
successMammalian Cell Lineapplication demo
Inferred from claim c3 during normalization. The authors established a stable cell line containing a genetically encoded PhyB-PIF light-inducible dimerization system. Derived from claim c3. Quoted text: successfully established a stable cell line containing a genetically encoded PhyB-PIF LID system
Source:
successMammalian Cell Lineapplication demo
Inferred from claim c3 during normalization. The authors established a stable cell line containing a genetically encoded PhyB-PIF light-inducible dimerization system. Derived from claim c3. Quoted text: successfully established a stable cell line containing a genetically encoded PhyB-PIF LID system
Source:
successMammalian Cell Lineapplication demo
Inferred from claim c3 during normalization. The authors established a stable cell line containing a genetically encoded PhyB-PIF light-inducible dimerization system. Derived from claim c3. Quoted text: successfully established a stable cell line containing a genetically encoded PhyB-PIF LID system
Source:
Supporting Sources
Ranked Claims
The PhyB-PIF light-inducible dimerization system controls both association and dissociation by light on the second time scale.
has a unique property of controlling both association and dissociation by light on the second time scale
response timescale second time scale
The PhyB-PIF light-inducible dimerization system controls both association and dissociation by light on the second time scale.
has a unique property of controlling both association and dissociation by light on the second time scale
response timescale second time scale
The PhyB-PIF light-inducible dimerization system controls both association and dissociation by light on the second time scale.
has a unique property of controlling both association and dissociation by light on the second time scale
response timescale second time scale
The PhyB-PIF light-inducible dimerization system controls both association and dissociation by light on the second time scale.
has a unique property of controlling both association and dissociation by light on the second time scale
response timescale second time scale
The PhyB-PIF light-inducible dimerization system controls both association and dissociation by light on the second time scale.
has a unique property of controlling both association and dissociation by light on the second time scale
response timescale second time scale
Drug inducible lentiviral and transposon vectors carrying PhyB-PIF and synPCB enabled doxycycline-inducible PCB synthesis and PhyB-PIF light-inducible dimerization system expression or function.
we incorporated PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors, which enabled us to induce PCB synthesis and PhyB-PIF LID system by doxycycline treatment
Drug inducible lentiviral and transposon vectors carrying PhyB-PIF and synPCB enabled doxycycline-inducible PCB synthesis and PhyB-PIF light-inducible dimerization system expression or function.
we incorporated PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors, which enabled us to induce PCB synthesis and PhyB-PIF LID system by doxycycline treatment
Drug inducible lentiviral and transposon vectors carrying PhyB-PIF and synPCB enabled doxycycline-inducible PCB synthesis and PhyB-PIF light-inducible dimerization system expression or function.
we incorporated PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors, which enabled us to induce PCB synthesis and PhyB-PIF LID system by doxycycline treatment
Drug inducible lentiviral and transposon vectors carrying PhyB-PIF and synPCB enabled doxycycline-inducible PCB synthesis and PhyB-PIF light-inducible dimerization system expression or function.
we incorporated PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors, which enabled us to induce PCB synthesis and PhyB-PIF LID system by doxycycline treatment
Drug inducible lentiviral and transposon vectors carrying PhyB-PIF and synPCB enabled doxycycline-inducible PCB synthesis and PhyB-PIF light-inducible dimerization system expression or function.
we incorporated PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors, which enabled us to induce PCB synthesis and PhyB-PIF LID system by doxycycline treatment
Drug inducible lentiviral and transposon vectors carrying PhyB-PIF and synPCB enabled doxycycline-inducible PCB synthesis and PhyB-PIF light-inducible dimerization system expression or function.
we incorporated PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors, which enabled us to induce PCB synthesis and PhyB-PIF LID system by doxycycline treatment
Drug inducible lentiviral and transposon vectors carrying PhyB-PIF and synPCB enabled doxycycline-inducible PCB synthesis and PhyB-PIF light-inducible dimerization system expression or function.
we incorporated PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors, which enabled us to induce PCB synthesis and PhyB-PIF LID system by doxycycline treatment
Drug inducible lentiviral and transposon vectors carrying PhyB-PIF and synPCB enabled doxycycline-inducible PCB synthesis and PhyB-PIF light-inducible dimerization system expression or function.
we incorporated PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors, which enabled us to induce PCB synthesis and PhyB-PIF LID system by doxycycline treatment
Drug inducible lentiviral and transposon vectors carrying PhyB-PIF and synPCB enabled doxycycline-inducible PCB synthesis and PhyB-PIF light-inducible dimerization system expression or function.
we incorporated PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors, which enabled us to induce PCB synthesis and PhyB-PIF LID system by doxycycline treatment
Drug inducible lentiviral and transposon vectors carrying PhyB-PIF and synPCB enabled doxycycline-inducible PCB synthesis and PhyB-PIF light-inducible dimerization system expression or function.
we incorporated PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors, which enabled us to induce PCB synthesis and PhyB-PIF LID system by doxycycline treatment
Drug inducible lentiviral and transposon vectors carrying PhyB-PIF and synPCB enabled doxycycline-inducible PCB synthesis and PhyB-PIF light-inducible dimerization system expression or function.
we incorporated PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors, which enabled us to induce PCB synthesis and PhyB-PIF LID system by doxycycline treatment
Drug inducible lentiviral and transposon vectors carrying PhyB-PIF and synPCB enabled doxycycline-inducible PCB synthesis and PhyB-PIF light-inducible dimerization system expression or function.
we incorporated PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors, which enabled us to induce PCB synthesis and PhyB-PIF LID system by doxycycline treatment
Drug inducible lentiviral and transposon vectors carrying PhyB-PIF and synPCB enabled doxycycline-inducible PCB synthesis and PhyB-PIF light-inducible dimerization system expression or function.
we incorporated PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors, which enabled us to induce PCB synthesis and PhyB-PIF LID system by doxycycline treatment
Drug inducible lentiviral and transposon vectors carrying PhyB-PIF and synPCB enabled doxycycline-inducible PCB synthesis and PhyB-PIF light-inducible dimerization system expression or function.
we incorporated PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors, which enabled us to induce PCB synthesis and PhyB-PIF LID system by doxycycline treatment
Drug inducible lentiviral and transposon vectors carrying PhyB-PIF and synPCB enabled doxycycline-inducible PCB synthesis and PhyB-PIF light-inducible dimerization system expression or function.
we incorporated PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors, which enabled us to induce PCB synthesis and PhyB-PIF LID system by doxycycline treatment
Drug inducible lentiviral and transposon vectors carrying PhyB-PIF and synPCB enabled doxycycline-inducible PCB synthesis and PhyB-PIF light-inducible dimerization system expression or function.
we incorporated PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors, which enabled us to induce PCB synthesis and PhyB-PIF LID system by doxycycline treatment
Drug inducible lentiviral and transposon vectors carrying PhyB-PIF and synPCB enabled doxycycline-inducible PCB synthesis and PhyB-PIF light-inducible dimerization system expression or function.
we incorporated PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors, which enabled us to induce PCB synthesis and PhyB-PIF LID system by doxycycline treatment
Drug inducible lentiviral and transposon vectors carrying PhyB-PIF and synPCB enabled doxycycline-inducible PCB synthesis and PhyB-PIF light-inducible dimerization system expression or function.
we incorporated PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors, which enabled us to induce PCB synthesis and PhyB-PIF LID system by doxycycline treatment
Drug inducible lentiviral and transposon vectors carrying PhyB-PIF and synPCB enabled doxycycline-inducible PCB synthesis and PhyB-PIF light-inducible dimerization system expression or function.
we incorporated PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors, which enabled us to induce PCB synthesis and PhyB-PIF LID system by doxycycline treatment
Drug inducible lentiviral and transposon vectors carrying PhyB-PIF and synPCB enabled doxycycline-inducible PCB synthesis and PhyB-PIF light-inducible dimerization system expression or function.
we incorporated PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors, which enabled us to induce PCB synthesis and PhyB-PIF LID system by doxycycline treatment
Drug inducible lentiviral and transposon vectors carrying PhyB-PIF and synPCB enabled doxycycline-inducible PCB synthesis and PhyB-PIF light-inducible dimerization system expression or function.
we incorporated PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors, which enabled us to induce PCB synthesis and PhyB-PIF LID system by doxycycline treatment
Drug inducible lentiviral and transposon vectors carrying PhyB-PIF and synPCB enabled doxycycline-inducible PCB synthesis and PhyB-PIF light-inducible dimerization system expression or function.
we incorporated PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors, which enabled us to induce PCB synthesis and PhyB-PIF LID system by doxycycline treatment
Drug inducible lentiviral and transposon vectors carrying PhyB-PIF and synPCB enabled doxycycline-inducible PCB synthesis and PhyB-PIF light-inducible dimerization system expression or function.
we incorporated PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors, which enabled us to induce PCB synthesis and PhyB-PIF LID system by doxycycline treatment
Drug inducible lentiviral and transposon vectors carrying PhyB-PIF and synPCB enabled doxycycline-inducible PCB synthesis and PhyB-PIF light-inducible dimerization system expression or function.
we incorporated PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors, which enabled us to induce PCB synthesis and PhyB-PIF LID system by doxycycline treatment
Drug inducible lentiviral and transposon vectors carrying PhyB-PIF and synPCB enabled doxycycline-inducible PCB synthesis and PhyB-PIF light-inducible dimerization system expression or function.
we incorporated PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors, which enabled us to induce PCB synthesis and PhyB-PIF LID system by doxycycline treatment
Drug inducible lentiviral and transposon vectors carrying PhyB-PIF and synPCB enabled doxycycline-inducible PCB synthesis and PhyB-PIF light-inducible dimerization system expression or function.
we incorporated PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors, which enabled us to induce PCB synthesis and PhyB-PIF LID system by doxycycline treatment
Drug inducible lentiviral and transposon vectors carrying PhyB-PIF and synPCB enabled doxycycline-inducible PCB synthesis and PhyB-PIF light-inducible dimerization system expression or function.
we incorporated PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors, which enabled us to induce PCB synthesis and PhyB-PIF LID system by doxycycline treatment
Drug inducible lentiviral and transposon vectors carrying PhyB-PIF and synPCB enabled doxycycline-inducible PCB synthesis and PhyB-PIF light-inducible dimerization system expression or function.
we incorporated PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors, which enabled us to induce PCB synthesis and PhyB-PIF LID system by doxycycline treatment
Drug inducible lentiviral and transposon vectors carrying PhyB-PIF and synPCB enabled doxycycline-inducible PCB synthesis and PhyB-PIF light-inducible dimerization system expression or function.
we incorporated PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors, which enabled us to induce PCB synthesis and PhyB-PIF LID system by doxycycline treatment
Incorporation of PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors enabled doxycycline-inducible PCB synthesis and PhyB-PIF light-inducible dimerization system expression or function.
we incorporated PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors, which enabled us to induce PCB synthesis and PhyB-PIF LID system by doxycycline treatment
Incorporation of PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors enabled doxycycline-inducible PCB synthesis and PhyB-PIF light-inducible dimerization system expression or function.
we incorporated PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors, which enabled us to induce PCB synthesis and PhyB-PIF LID system by doxycycline treatment
Incorporation of PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors enabled doxycycline-inducible PCB synthesis and PhyB-PIF light-inducible dimerization system expression or function.
we incorporated PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors, which enabled us to induce PCB synthesis and PhyB-PIF LID system by doxycycline treatment
Incorporation of PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors enabled doxycycline-inducible PCB synthesis and PhyB-PIF light-inducible dimerization system expression or function.
we incorporated PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors, which enabled us to induce PCB synthesis and PhyB-PIF LID system by doxycycline treatment
Incorporation of PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors enabled doxycycline-inducible PCB synthesis and PhyB-PIF light-inducible dimerization system expression or function.
we incorporated PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors, which enabled us to induce PCB synthesis and PhyB-PIF LID system by doxycycline treatment
Incorporation of PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors enabled doxycycline-inducible PCB synthesis and PhyB-PIF light-inducible dimerization system expression or function.
we incorporated PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors, which enabled us to induce PCB synthesis and PhyB-PIF LID system by doxycycline treatment
Incorporation of PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors enabled doxycycline-inducible PCB synthesis and PhyB-PIF light-inducible dimerization system expression or function.
we incorporated PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors, which enabled us to induce PCB synthesis and PhyB-PIF LID system by doxycycline treatment
Incorporation of PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors enabled doxycycline-inducible PCB synthesis and PhyB-PIF light-inducible dimerization system expression or function.
we incorporated PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors, which enabled us to induce PCB synthesis and PhyB-PIF LID system by doxycycline treatment
Incorporation of PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors enabled doxycycline-inducible PCB synthesis and PhyB-PIF light-inducible dimerization system expression or function.
we incorporated PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors, which enabled us to induce PCB synthesis and PhyB-PIF LID system by doxycycline treatment
Incorporation of PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors enabled doxycycline-inducible PCB synthesis and PhyB-PIF light-inducible dimerization system expression or function.
we incorporated PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors, which enabled us to induce PCB synthesis and PhyB-PIF LID system by doxycycline treatment
Concatenating the PCB synthesis genes with P2A peptide cDNAs for polycistronic expression resulted in an approximately 4-fold increase in PCB synthesis compared with the previous version.
these genes were concatenated with P2A peptide cDNAs for polycistronic expression, resulting in an approximately 4-fold increase in PCB synthesis compared with the previous version
PCB synthesis increase 4 fold
Concatenating the PCB synthesis genes with P2A peptide cDNAs for polycistronic expression resulted in an approximately 4-fold increase in PCB synthesis compared with the previous version.
these genes were concatenated with P2A peptide cDNAs for polycistronic expression, resulting in an approximately 4-fold increase in PCB synthesis compared with the previous version
PCB synthesis increase 4 fold
Concatenating the PCB synthesis genes with P2A peptide cDNAs for polycistronic expression resulted in an approximately 4-fold increase in PCB synthesis compared with the previous version.
these genes were concatenated with P2A peptide cDNAs for polycistronic expression, resulting in an approximately 4-fold increase in PCB synthesis compared with the previous version
PCB synthesis increase 4 fold
Concatenating the PCB synthesis genes with P2A peptide cDNAs for polycistronic expression resulted in an approximately 4-fold increase in PCB synthesis compared with the previous version.
these genes were concatenated with P2A peptide cDNAs for polycistronic expression, resulting in an approximately 4-fold increase in PCB synthesis compared with the previous version
PCB synthesis increase 4 fold
Concatenating the PCB synthesis genes with P2A peptide cDNAs for polycistronic expression resulted in an approximately 4-fold increase in PCB synthesis compared with the previous version.
these genes were concatenated with P2A peptide cDNAs for polycistronic expression, resulting in an approximately 4-fold increase in PCB synthesis compared with the previous version
PCB synthesis increase 4 fold
The PhyB-PIF red/far-red responsive light-inducible dimerization system requires a linear tetrapyrrole chromophore such as phytochromobilin or phycocyanobilin.
PhyB requires a linear tetrapyrrole chromophore such as phytochromobilin or phycocyanobilin (PCB)
The PhyB-PIF red/far-red responsive light-inducible dimerization system requires a linear tetrapyrrole chromophore such as phytochromobilin or phycocyanobilin.
PhyB requires a linear tetrapyrrole chromophore such as phytochromobilin or phycocyanobilin (PCB)
The PhyB-PIF red/far-red responsive light-inducible dimerization system requires a linear tetrapyrrole chromophore such as phytochromobilin or phycocyanobilin.
PhyB requires a linear tetrapyrrole chromophore such as phytochromobilin or phycocyanobilin (PCB)
The PhyB-PIF red/far-red responsive light-inducible dimerization system requires a linear tetrapyrrole chromophore such as phytochromobilin or phycocyanobilin.
PhyB requires a linear tetrapyrrole chromophore such as phytochromobilin or phycocyanobilin (PCB)
The PhyB-PIF red/far-red responsive light-inducible dimerization system requires a linear tetrapyrrole chromophore such as phytochromobilin or phycocyanobilin.
PhyB requires a linear tetrapyrrole chromophore such as phytochromobilin or phycocyanobilin (PCB)
The PhyB-PIF red/far-red responsive light-inducible dimerization system requires a linear tetrapyrrole chromophore such as phytochromobilin or phycocyanobilin.
PhyB requires a linear tetrapyrrole chromophore such as phytochromobilin or phycocyanobilin (PCB)
The PhyB-PIF red/far-red responsive light-inducible dimerization system requires a linear tetrapyrrole chromophore such as phytochromobilin or phycocyanobilin.
PhyB requires a linear tetrapyrrole chromophore such as phytochromobilin or phycocyanobilin (PCB)
The PhyB-PIF red/far-red responsive light-inducible dimerization system requires a linear tetrapyrrole chromophore such as phytochromobilin or phycocyanobilin.
PhyB requires a linear tetrapyrrole chromophore such as phytochromobilin or phycocyanobilin (PCB)
The PhyB-PIF red/far-red responsive light-inducible dimerization system requires a linear tetrapyrrole chromophore such as phytochromobilin or phycocyanobilin.
PhyB requires a linear tetrapyrrole chromophore such as phytochromobilin or phycocyanobilin (PCB)
The PhyB-PIF red/far-red responsive light-inducible dimerization system requires a linear tetrapyrrole chromophore such as phytochromobilin or phycocyanobilin.
PhyB requires a linear tetrapyrrole chromophore such as phytochromobilin or phycocyanobilin (PCB)
The authors established a stable cell line containing a genetically encoded PhyB-PIF light-inducible dimerization system.
successfully established a stable cell line containing a genetically encoded PhyB-PIF LID system
The authors established a stable cell line containing a genetically encoded PhyB-PIF light-inducible dimerization system.
successfully established a stable cell line containing a genetically encoded PhyB-PIF LID system
The authors established a stable cell line containing a genetically encoded PhyB-PIF light-inducible dimerization system.
successfully established a stable cell line containing a genetically encoded PhyB-PIF LID system
The authors established a stable cell line containing a genetically encoded PhyB-PIF light-inducible dimerization system.
successfully established a stable cell line containing a genetically encoded PhyB-PIF LID system
The authors established a stable cell line containing a genetically encoded PhyB-PIF light-inducible dimerization system.
successfully established a stable cell line containing a genetically encoded PhyB-PIF LID system
The authors established a stable cell line containing a genetically encoded PhyB-PIF light-inducible dimerization system.
successfully established a stable cell line containing a genetically encoded PhyB-PIF LID system
The authors established a stable cell line containing a genetically encoded PhyB-PIF light-inducible dimerization system.
successfully established a stable cell line containing a genetically encoded PhyB-PIF LID system
The authors established a stable cell line containing a genetically encoded PhyB-PIF light-inducible dimerization system.
successfully established a stable cell line containing a genetically encoded PhyB-PIF LID system
The authors established a stable cell line containing a genetically encoded PhyB-PIF light-inducible dimerization system.
successfully established a stable cell line containing a genetically encoded PhyB-PIF LID system
The authors established a stable cell line containing a genetically encoded PhyB-PIF light-inducible dimerization system.
successfully established a stable cell line containing a genetically encoded PhyB-PIF LID system
The authors established a stable cell line containing a genetically encoded PhyB-PIF light-inducible dimerization system.
successfully established a stable cell line containing a genetically encoded PhyB-PIF LID system
The authors established a stable cell line containing a genetically encoded PhyB-PIF light-inducible dimerization system.
successfully established a stable cell line containing a genetically encoded PhyB-PIF LID system
The authors established a stable cell line containing a genetically encoded PhyB-PIF light-inducible dimerization system.
successfully established a stable cell line containing a genetically encoded PhyB-PIF LID system
The authors established a stable cell line containing a genetically encoded PhyB-PIF light-inducible dimerization system.
successfully established a stable cell line containing a genetically encoded PhyB-PIF LID system
The authors established a stable cell line containing a genetically encoded PhyB-PIF light-inducible dimerization system.
successfully established a stable cell line containing a genetically encoded PhyB-PIF LID system
The authors established a stable cell line containing a genetically encoded PhyB-PIF light-inducible dimerization system.
successfully established a stable cell line containing a genetically encoded PhyB-PIF LID system
The authors established a stable cell line containing a genetically encoded PhyB-PIF light-inducible dimerization system.
successfully established a stable cell line containing a genetically encoded PhyB-PIF LID system
The authors established a stable cell line containing a genetically encoded PhyB-PIF light-inducible dimerization system.
successfully established a stable cell line containing a genetically encoded PhyB-PIF LID system
The authors established a stable cell line containing a genetically encoded PhyB-PIF light-inducible dimerization system.
successfully established a stable cell line containing a genetically encoded PhyB-PIF LID system
The authors established a stable cell line containing a genetically encoded PhyB-PIF light-inducible dimerization system.
successfully established a stable cell line containing a genetically encoded PhyB-PIF LID system
The authors established a stable cell line containing a genetically encoded PhyB-PIF light-inducible dimerization system.
successfully established a stable cell line containing a genetically encoded PhyB-PIF LID system
The authors established a stable cell line containing a genetically encoded PhyB-PIF light-inducible dimerization system.
successfully established a stable cell line containing a genetically encoded PhyB-PIF LID system
The authors established a stable cell line containing a genetically encoded PhyB-PIF light-inducible dimerization system.
successfully established a stable cell line containing a genetically encoded PhyB-PIF LID system
The authors established a stable cell line containing a genetically encoded PhyB-PIF light-inducible dimerization system.
successfully established a stable cell line containing a genetically encoded PhyB-PIF LID system
The authors established a stable cell line containing a genetically encoded PhyB-PIF light-inducible dimerization system.
successfully established a stable cell line containing a genetically encoded PhyB-PIF LID system
The authors established a stable cell line containing a genetically encoded PhyB-PIF light-inducible dimerization system.
successfully established a stable cell line containing a genetically encoded PhyB-PIF LID system
The authors established a stable cell line containing a genetically encoded PhyB-PIF light-inducible dimerization system.
successfully established a stable cell line containing a genetically encoded PhyB-PIF LID system
The authors established a stable cell line containing a genetically encoded PhyB-PIF light-inducible dimerization system.
successfully established a stable cell line containing a genetically encoded PhyB-PIF LID system
The authors established a stable cell line containing a genetically encoded PhyB-PIF light-inducible dimerization system.
successfully established a stable cell line containing a genetically encoded PhyB-PIF LID system
The authors successfully established a stable cell line containing a genetically encoded PhyB-PIF light-inducible dimerization system.
successfully established a stable cell line containing a genetically encoded PhyB-PIF LID system
The authors successfully established a stable cell line containing a genetically encoded PhyB-PIF light-inducible dimerization system.
successfully established a stable cell line containing a genetically encoded PhyB-PIF LID system
The authors successfully established a stable cell line containing a genetically encoded PhyB-PIF light-inducible dimerization system.
successfully established a stable cell line containing a genetically encoded PhyB-PIF LID system
The authors successfully established a stable cell line containing a genetically encoded PhyB-PIF light-inducible dimerization system.
successfully established a stable cell line containing a genetically encoded PhyB-PIF LID system
The authors successfully established a stable cell line containing a genetically encoded PhyB-PIF light-inducible dimerization system.
successfully established a stable cell line containing a genetically encoded PhyB-PIF LID system
The authors successfully established a stable cell line containing a genetically encoded PhyB-PIF light-inducible dimerization system.
successfully established a stable cell line containing a genetically encoded PhyB-PIF LID system
The authors successfully established a stable cell line containing a genetically encoded PhyB-PIF light-inducible dimerization system.
successfully established a stable cell line containing a genetically encoded PhyB-PIF LID system
The authors successfully established a stable cell line containing a genetically encoded PhyB-PIF light-inducible dimerization system.
successfully established a stable cell line containing a genetically encoded PhyB-PIF LID system
The authors successfully established a stable cell line containing a genetically encoded PhyB-PIF light-inducible dimerization system.
successfully established a stable cell line containing a genetically encoded PhyB-PIF LID system
The authors successfully established a stable cell line containing a genetically encoded PhyB-PIF light-inducible dimerization system.
successfully established a stable cell line containing a genetically encoded PhyB-PIF LID system
The improved synPCB design increased PCB synthesis by approximately 4-fold compared with the previous version.
resulting in an approximately 4-fold increase in PCB synthesis compared with the previous version
PCB synthesis increase versus previous version approximately 4-fold
The improved synPCB design increased PCB synthesis by approximately 4-fold compared with the previous version.
resulting in an approximately 4-fold increase in PCB synthesis compared with the previous version
PCB synthesis increase versus previous version approximately 4-fold
The improved synPCB design increased PCB synthesis by approximately 4-fold compared with the previous version.
resulting in an approximately 4-fold increase in PCB synthesis compared with the previous version
PCB synthesis increase versus previous version approximately 4-fold
The improved synPCB design increased PCB synthesis by approximately 4-fold compared with the previous version.
resulting in an approximately 4-fold increase in PCB synthesis compared with the previous version
PCB synthesis increase versus previous version approximately 4-fold
The improved synPCB design increased PCB synthesis by approximately 4-fold compared with the previous version.
resulting in an approximately 4-fold increase in PCB synthesis compared with the previous version
PCB synthesis increase versus previous version approximately 4-fold
The improved synPCB design increased PCB synthesis by approximately 4-fold compared with the previous version.
resulting in an approximately 4-fold increase in PCB synthesis compared with the previous version
PCB synthesis increase versus previous version approximately 4-fold
The improved synPCB design increased PCB synthesis by approximately 4-fold compared with the previous version.
resulting in an approximately 4-fold increase in PCB synthesis compared with the previous version
PCB synthesis increase versus previous version approximately 4-fold
The improved synPCB design increased PCB synthesis by approximately 4-fold compared with the previous version.
resulting in an approximately 4-fold increase in PCB synthesis compared with the previous version
PCB synthesis increase versus previous version approximately 4-fold
The improved synPCB design increased PCB synthesis by approximately 4-fold compared with the previous version.
resulting in an approximately 4-fold increase in PCB synthesis compared with the previous version
PCB synthesis increase versus previous version approximately 4-fold
The improved synPCB design increased PCB synthesis by approximately 4-fold compared with the previous version.
resulting in an approximately 4-fold increase in PCB synthesis compared with the previous version
PCB synthesis increase versus previous version approximately 4-fold
The improved synPCB design increased PCB synthesis by approximately 4-fold compared with the previous version.
resulting in an approximately 4-fold increase in PCB synthesis compared with the previous version
PCB synthesis increase versus previous version approximately 4-fold
The improved synPCB design increased PCB synthesis by approximately 4-fold compared with the previous version.
resulting in an approximately 4-fold increase in PCB synthesis compared with the previous version
PCB synthesis increase versus previous version approximately 4-fold
The improved synPCB design increased PCB synthesis by approximately 4-fold compared with the previous version.
resulting in an approximately 4-fold increase in PCB synthesis compared with the previous version
PCB synthesis increase versus previous version approximately 4-fold
The improved synPCB design increased PCB synthesis by approximately 4-fold compared with the previous version.
resulting in an approximately 4-fold increase in PCB synthesis compared with the previous version
PCB synthesis increase versus previous version approximately 4-fold
The improved synPCB design increased PCB synthesis by approximately 4-fold compared with the previous version.
resulting in an approximately 4-fold increase in PCB synthesis compared with the previous version
PCB synthesis increase versus previous version approximately 4-fold
Approval Evidence
1 source2 linked approval claimsfirst-pass slug drug-inducible-lentiviral-and-transposon-vectors
we incorporated PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors, which enabled us to induce PCB synthesis and PhyB-PIF LID system by doxycycline treatment
Source:
inducible controlsupports
Drug inducible lentiviral and transposon vectors carrying PhyB-PIF and synPCB enabled doxycycline-inducible PCB synthesis and PhyB-PIF light-inducible dimerization system expression or function.
we incorporated PhyB-PIF and synPCB into drug inducible lentiviral and transposon vectors, which enabled us to induce PCB synthesis and PhyB-PIF LID system by doxycycline treatment
Source:
system establishmentsupports
The authors established a stable cell line containing a genetically encoded PhyB-PIF light-inducible dimerization system.
successfully established a stable cell line containing a genetically encoded PhyB-PIF LID system
Source:
Comparisons
Source-stated alternatives
The source mentions both lentiviral and transposon vectors as delivery formats used for inducible implementation.
Source:
The source mentions both lentiviral and transposon vectors as delivery formats used for inducible implementation.
Source-backed strengths
The cited work states that these inducible lentiviral and transposon vectors enabled doxycycline-inducible PCB synthesis and PhyB-PIF light-inducible dimerization system activity. The associated PhyB-PIF system is described as controlling both association and dissociation by light on the second time scale.
Source:
supports doxycycline-inducible control
Source:
enabled establishment of a stable cell line
Compared with LightOn system
drug inducible lentiviral and transposon vectors and LightOn system address a similar problem space.
Shared frame: shared mechanisms: heterodimerization; same primary input modality: light
Strengths here: looks easier to implement in practice.
Relative tradeoffs: may avoid an exogenous cofactor requirement.
Compared with split-TurboID
drug inducible lentiviral and transposon vectors and split-TurboID address a similar problem space.
Shared frame: shared mechanisms: heterodimerization; same primary input modality: light
Relative tradeoffs: appears more independently replicated; looks easier to implement in practice; may avoid an exogenous cofactor requirement.
Compared with tandem-dimer nano (tdnano)
drug inducible lentiviral and transposon vectors and tandem-dimer nano (tdnano) address a similar problem space.
Shared frame: shared mechanisms: heterodimerization; same primary input modality: light
Strengths here: looks easier to implement in practice.
Relative tradeoffs: may avoid an exogenous cofactor requirement.
Ranked Citations