Source 1primary paper2004Journal of Bacteriology
Toolkit/DspA
DspA
Also known as: hik33
Taxonomy: Mechanism Branch / Component. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
DspA, also called Hik33, is a sensor histidine kinase from Synechocystis sp. strain PCC6803. It functions in signal transduction that controls photosynthetic and high-light-responsive gene sets and contributes to adaptation of cellular metabolism and growth to environmental light conditions.
Usefulness & Problems
Why this is useful
DspA is useful as a native cyanobacterial signaling component for studying how environmental conditions are coupled to transcriptional programs affecting photosynthesis and high-light responses. The cited evidence supports its relevance for interrogating light-dependent growth regulation in Synechocystis sp. strain PCC6803, but does not provide engineering performance data.
Problem solved
DspA helps address the biological problem of coordinating photosynthetic and high-light-responsive gene expression with environmental constraints on growth. In Synechocystis sp. strain PCC6803, it is implicated in integrating signals that shape photoautotrophic growth under low and moderate light.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Component: A low-level protein part used inside a larger architecture that realizes a mechanism.
Mechanisms
transcriptional regulationtranscriptional regulation of photosynthetic and high-light-responsive genestwo-component histidine kinase signalingTechniques
Directed EvolutionTarget processes
recombinationsignalingInput: Light
Implementation Constraints
The supplied evidence supports that DspA is a sensor histidine kinase from Synechocystis sp. strain PCC6803 and that its deletion alters photoautotrophic growth. However, no practical implementation details such as cofactors, expression strategies, fusion design, or delivery methods are described in the provided material.
The evidence is limited to one cited study in Synechocystis sp. strain PCC6803 and does not establish DspA as an engineered optogenetic or synthetic biology tool. No quantitative activation parameters, domain architecture details, spectral properties, construct designs, or cross-species validation are provided in the supplied evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
DspA is involved in controlling sets of photosynthetic and high-light-responsive genes in Synechocystis sp. strain PCC6803.
These results suggest that DspA is involved in controlling sets of photosynthetic and high-light-responsive genes, either directly or indirectly.
DspA is involved in controlling sets of photosynthetic and high-light-responsive genes in Synechocystis sp. strain PCC6803.
These results suggest that DspA is involved in controlling sets of photosynthetic and high-light-responsive genes, either directly or indirectly.
DspA is involved in controlling sets of photosynthetic and high-light-responsive genes in Synechocystis sp. strain PCC6803.
These results suggest that DspA is involved in controlling sets of photosynthetic and high-light-responsive genes, either directly or indirectly.
DspA is involved in controlling sets of photosynthetic and high-light-responsive genes in Synechocystis sp. strain PCC6803.
These results suggest that DspA is involved in controlling sets of photosynthetic and high-light-responsive genes, either directly or indirectly.
DspA is involved in controlling sets of photosynthetic and high-light-responsive genes in Synechocystis sp. strain PCC6803.
These results suggest that DspA is involved in controlling sets of photosynthetic and high-light-responsive genes, either directly or indirectly.
DspA is involved in controlling sets of photosynthetic and high-light-responsive genes in Synechocystis sp. strain PCC6803.
These results suggest that DspA is involved in controlling sets of photosynthetic and high-light-responsive genes, either directly or indirectly.
DspA is involved in controlling sets of photosynthetic and high-light-responsive genes in Synechocystis sp. strain PCC6803.
These results suggest that DspA is involved in controlling sets of photosynthetic and high-light-responsive genes, either directly or indirectly.
DspA acts as a global regulator that helps coordinate cellular metabolism with growth limitations imposed by environmental conditions.
suggest that DspA acts as a global regulator that helps coordinate cellular metabolism with growth limitations imposed by environmental conditions
DspA acts as a global regulator that helps coordinate cellular metabolism with growth limitations imposed by environmental conditions.
suggest that DspA acts as a global regulator that helps coordinate cellular metabolism with growth limitations imposed by environmental conditions
DspA acts as a global regulator that helps coordinate cellular metabolism with growth limitations imposed by environmental conditions.
suggest that DspA acts as a global regulator that helps coordinate cellular metabolism with growth limitations imposed by environmental conditions
DspA acts as a global regulator that helps coordinate cellular metabolism with growth limitations imposed by environmental conditions.
suggest that DspA acts as a global regulator that helps coordinate cellular metabolism with growth limitations imposed by environmental conditions
DspA acts as a global regulator that helps coordinate cellular metabolism with growth limitations imposed by environmental conditions.
suggest that DspA acts as a global regulator that helps coordinate cellular metabolism with growth limitations imposed by environmental conditions
DspA acts as a global regulator that helps coordinate cellular metabolism with growth limitations imposed by environmental conditions.
suggest that DspA acts as a global regulator that helps coordinate cellular metabolism with growth limitations imposed by environmental conditions
DspA acts as a global regulator that helps coordinate cellular metabolism with growth limitations imposed by environmental conditions.
suggest that DspA acts as a global regulator that helps coordinate cellular metabolism with growth limitations imposed by environmental conditions
Deletion of dspA causes severely reduced photosynthetic oxygen evolution in low and moderate light under photoautotrophic conditions.
and had severely reduced photosynthetic oxygen evolution
Deletion of dspA causes severely reduced photosynthetic oxygen evolution in low and moderate light under photoautotrophic conditions.
and had severely reduced photosynthetic oxygen evolution
Deletion of dspA causes severely reduced photosynthetic oxygen evolution in low and moderate light under photoautotrophic conditions.
and had severely reduced photosynthetic oxygen evolution
Deletion of dspA causes severely reduced photosynthetic oxygen evolution in low and moderate light under photoautotrophic conditions.
and had severely reduced photosynthetic oxygen evolution
Deletion of dspA causes severely reduced photosynthetic oxygen evolution in low and moderate light under photoautotrophic conditions.
and had severely reduced photosynthetic oxygen evolution
Deletion of dspA causes severely reduced photosynthetic oxygen evolution in low and moderate light under photoautotrophic conditions.
and had severely reduced photosynthetic oxygen evolution
Deletion of dspA causes severely reduced photosynthetic oxygen evolution in low and moderate light under photoautotrophic conditions.
and had severely reduced photosynthetic oxygen evolution
Deletion of dspA in Synechocystis sp. strain PCC6803 causes slow photoautotrophic growth in low and moderate light.
In low and moderate light, the mutant grew slowly under photoautotrophic conditions, with a doubling time of approximately 40 h
doubling time 40 h
Deletion of dspA in Synechocystis sp. strain PCC6803 causes slow photoautotrophic growth in low and moderate light.
In low and moderate light, the mutant grew slowly under photoautotrophic conditions, with a doubling time of approximately 40 h
doubling time 40 h
Deletion of dspA in Synechocystis sp. strain PCC6803 causes slow photoautotrophic growth in low and moderate light.
In low and moderate light, the mutant grew slowly under photoautotrophic conditions, with a doubling time of approximately 40 h
doubling time 40 h
Deletion of dspA in Synechocystis sp. strain PCC6803 causes slow photoautotrophic growth in low and moderate light.
In low and moderate light, the mutant grew slowly under photoautotrophic conditions, with a doubling time of approximately 40 h
doubling time 40 h
Deletion of dspA in Synechocystis sp. strain PCC6803 causes slow photoautotrophic growth in low and moderate light.
In low and moderate light, the mutant grew slowly under photoautotrophic conditions, with a doubling time of approximately 40 h
doubling time 40 h
Deletion of dspA in Synechocystis sp. strain PCC6803 causes slow photoautotrophic growth in low and moderate light.
In low and moderate light, the mutant grew slowly under photoautotrophic conditions, with a doubling time of approximately 40 h
doubling time 40 h
Deletion of dspA in Synechocystis sp. strain PCC6803 causes slow photoautotrophic growth in low and moderate light.
In low and moderate light, the mutant grew slowly under photoautotrophic conditions, with a doubling time of approximately 40 h
doubling time 40 h
The dspA mutant is light sensitive and rapidly dies in high light.
However, the mutant was light sensitive and rapidly died in high light.
The dspA mutant is light sensitive and rapidly dies in high light.
However, the mutant was light sensitive and rapidly died in high light.
The dspA mutant is light sensitive and rapidly dies in high light.
However, the mutant was light sensitive and rapidly died in high light.
The dspA mutant is light sensitive and rapidly dies in high light.
However, the mutant was light sensitive and rapidly died in high light.
The dspA mutant is light sensitive and rapidly dies in high light.
However, the mutant was light sensitive and rapidly died in high light.
The dspA mutant is light sensitive and rapidly dies in high light.
However, the mutant was light sensitive and rapidly died in high light.
The dspA mutant is light sensitive and rapidly dies in high light.
However, the mutant was light sensitive and rapidly died in high light.
In low light in the presence of glucose, the dspA mutant fails to accumulate cpcBA and psaAB mRNAs.
the cells failed to accumulate cpcBA and psaAB mRNAs in low light in the presence of glucose
In low light in the presence of glucose, the dspA mutant fails to accumulate cpcBA and psaAB mRNAs.
the cells failed to accumulate cpcBA and psaAB mRNAs in low light in the presence of glucose
In low light in the presence of glucose, the dspA mutant fails to accumulate cpcBA and psaAB mRNAs.
the cells failed to accumulate cpcBA and psaAB mRNAs in low light in the presence of glucose
In low light in the presence of glucose, the dspA mutant fails to accumulate cpcBA and psaAB mRNAs.
the cells failed to accumulate cpcBA and psaAB mRNAs in low light in the presence of glucose
In low light in the presence of glucose, the dspA mutant fails to accumulate cpcBA and psaAB mRNAs.
the cells failed to accumulate cpcBA and psaAB mRNAs in low light in the presence of glucose
In low light in the presence of glucose, the dspA mutant fails to accumulate cpcBA and psaAB mRNAs.
the cells failed to accumulate cpcBA and psaAB mRNAs in low light in the presence of glucose
In low light in the presence of glucose, the dspA mutant fails to accumulate cpcBA and psaAB mRNAs.
the cells failed to accumulate cpcBA and psaAB mRNAs in low light in the presence of glucose
In the dspA mutant, transcript levels of many genes associated with photosynthesis are altered relative to wild type in both low light and after exposure to high light.
levels of many transcripts encoding genes associated with photosynthesis were altered in the mutant relative to wild-type Synechocystis sp. strain PCC6803 both in low light and following exposure to high light
In the dspA mutant, transcript levels of many genes associated with photosynthesis are altered relative to wild type in both low light and after exposure to high light.
levels of many transcripts encoding genes associated with photosynthesis were altered in the mutant relative to wild-type Synechocystis sp. strain PCC6803 both in low light and following exposure to high light
In the dspA mutant, transcript levels of many genes associated with photosynthesis are altered relative to wild type in both low light and after exposure to high light.
levels of many transcripts encoding genes associated with photosynthesis were altered in the mutant relative to wild-type Synechocystis sp. strain PCC6803 both in low light and following exposure to high light
In the dspA mutant, transcript levels of many genes associated with photosynthesis are altered relative to wild type in both low light and after exposure to high light.
levels of many transcripts encoding genes associated with photosynthesis were altered in the mutant relative to wild-type Synechocystis sp. strain PCC6803 both in low light and following exposure to high light
In the dspA mutant, transcript levels of many genes associated with photosynthesis are altered relative to wild type in both low light and after exposure to high light.
levels of many transcripts encoding genes associated with photosynthesis were altered in the mutant relative to wild-type Synechocystis sp. strain PCC6803 both in low light and following exposure to high light
In the dspA mutant, transcript levels of many genes associated with photosynthesis are altered relative to wild type in both low light and after exposure to high light.
levels of many transcripts encoding genes associated with photosynthesis were altered in the mutant relative to wild-type Synechocystis sp. strain PCC6803 both in low light and following exposure to high light
In the dspA mutant, transcript levels of many genes associated with photosynthesis are altered relative to wild type in both low light and after exposure to high light.
levels of many transcripts encoding genes associated with photosynthesis were altered in the mutant relative to wild-type Synechocystis sp. strain PCC6803 both in low light and following exposure to high light
The dspA mutant shows constitutive expression of several high-light-inducible genes including hli, psbAIII, and gpx2.
There was constitutive expression of several high-light-inducible genes, including hli, psbAIII, and gpx2
The dspA mutant shows constitutive expression of several high-light-inducible genes including hli, psbAIII, and gpx2.
There was constitutive expression of several high-light-inducible genes, including hli, psbAIII, and gpx2
The dspA mutant shows constitutive expression of several high-light-inducible genes including hli, psbAIII, and gpx2.
There was constitutive expression of several high-light-inducible genes, including hli, psbAIII, and gpx2
The dspA mutant shows constitutive expression of several high-light-inducible genes including hli, psbAIII, and gpx2.
There was constitutive expression of several high-light-inducible genes, including hli, psbAIII, and gpx2
The dspA mutant shows constitutive expression of several high-light-inducible genes including hli, psbAIII, and gpx2.
There was constitutive expression of several high-light-inducible genes, including hli, psbAIII, and gpx2
The dspA mutant shows constitutive expression of several high-light-inducible genes including hli, psbAIII, and gpx2.
There was constitutive expression of several high-light-inducible genes, including hli, psbAIII, and gpx2
The dspA mutant shows constitutive expression of several high-light-inducible genes including hli, psbAIII, and gpx2.
There was constitutive expression of several high-light-inducible genes, including hli, psbAIII, and gpx2
The dspA mutant shows little increased accumulation of sodB mRNA in high light.
there was little increased accumulation of sodB mRNA in high light
The dspA mutant shows little increased accumulation of sodB mRNA in high light.
there was little increased accumulation of sodB mRNA in high light
The dspA mutant shows little increased accumulation of sodB mRNA in high light.
there was little increased accumulation of sodB mRNA in high light
The dspA mutant shows little increased accumulation of sodB mRNA in high light.
there was little increased accumulation of sodB mRNA in high light
The dspA mutant shows little increased accumulation of sodB mRNA in high light.
there was little increased accumulation of sodB mRNA in high light
The dspA mutant shows little increased accumulation of sodB mRNA in high light.
there was little increased accumulation of sodB mRNA in high light
The dspA mutant shows little increased accumulation of sodB mRNA in high light.
there was little increased accumulation of sodB mRNA in high light
Approval Evidence
1 source9 linked approval claimsfirst-pass slug dspa
a sensor histidine kinase, dspA (or hik33)
Source:
gene functionsupports
DspA is involved in controlling sets of photosynthetic and high-light-responsive genes in Synechocystis sp. strain PCC6803.
These results suggest that DspA is involved in controlling sets of photosynthetic and high-light-responsive genes, either directly or indirectly.
Source:
global regulationsupports
DspA acts as a global regulator that helps coordinate cellular metabolism with growth limitations imposed by environmental conditions.
suggest that DspA acts as a global regulator that helps coordinate cellular metabolism with growth limitations imposed by environmental conditions
Source:
mutant phenotypesupports
Deletion of dspA causes severely reduced photosynthetic oxygen evolution in low and moderate light under photoautotrophic conditions.
and had severely reduced photosynthetic oxygen evolution
Source:
mutant phenotypesupports
Deletion of dspA in Synechocystis sp. strain PCC6803 causes slow photoautotrophic growth in low and moderate light.
In low and moderate light, the mutant grew slowly under photoautotrophic conditions, with a doubling time of approximately 40 h
Source:
mutant phenotypesupports
The dspA mutant is light sensitive and rapidly dies in high light.
However, the mutant was light sensitive and rapidly died in high light.
Source:
transcript regulationsupports
In low light in the presence of glucose, the dspA mutant fails to accumulate cpcBA and psaAB mRNAs.
the cells failed to accumulate cpcBA and psaAB mRNAs in low light in the presence of glucose
Source:
transcript regulationsupports
In the dspA mutant, transcript levels of many genes associated with photosynthesis are altered relative to wild type in both low light and after exposure to high light.
levels of many transcripts encoding genes associated with photosynthesis were altered in the mutant relative to wild-type Synechocystis sp. strain PCC6803 both in low light and following exposure to high light
Source:
transcript regulationsupports
The dspA mutant shows constitutive expression of several high-light-inducible genes including hli, psbAIII, and gpx2.
There was constitutive expression of several high-light-inducible genes, including hli, psbAIII, and gpx2
Source:
transcript regulationsupports
The dspA mutant shows little increased accumulation of sodB mRNA in high light.
there was little increased accumulation of sodB mRNA in high light
Source:
Comparisons
Source-backed strengths
The available evidence identifies DspA as a global regulator rather than a narrowly acting factor, indicating system-level influence on cellular metabolism and gene expression. Functional relevance is supported by a deletion phenotype, as loss of dspA causes slow photoautotrophic growth in low and moderate light.
Ranked Citations
- 1.