Source 1primary paper2023MED
Toolkit/eGAV
eGAV
Also known as: enhanced Gal4-VVD transcription factor
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
eGAV (enhanced Gal4-VVD transcription factor) is a light-controlled transcription switch for mammalian and vertebrate systems. It mediates inducible gene expression and has been validated in cultured HEK293T and mouse EpH4 cells, as well as in mouse brain, chick spinal cord, and adult mouse hepatocytes.
Usefulness & Problems
Why this is useful
eGAV is useful for optically controlling transgene expression across multiple mammalian and vertebrate experimental contexts. The available evidence indicates applicability in transiently transfected cultured cells, lentivirally transduced cells, and several in vivo tissues.
Source:
eGAV-mediated light-controlled gene expression was verified in cultured HEK293T cells by plasmid transient transfection and in mouse EpH4 cells by lentiviral vector-mediated transduction.
Source:
eGAV-mediated light-controlled transcription was confirmed in neural stem cells and progenitors in developing and adult mouse brain and chick spinal cord, and in adult mouse hepatocytes, indicating applicability across a wide range of experimental systems and model organisms.
Source:
eGAV is a new optimized photo-activatable Gal4 transcription factor developed by optimizing Gal4 DNA-binding domain, transcription activation domain, and VVD-based light-dependent dimerization configurations.
Problem solved
eGAV helps solve the problem of inducing gene expression with light in systems where spatial and temporal control of transcription is needed. The cited study specifically supports its use across diverse cell types and tissues rather than restricting use to a single model.
Source:
eGAV-mediated light-controlled gene expression was verified in cultured HEK293T cells by plasmid transient transfection and in mouse EpH4 cells by lentiviral vector-mediated transduction.
Source:
eGAV-mediated light-controlled transcription was confirmed in neural stem cells and progenitors in developing and adult mouse brain and chick spinal cord, and in adult mouse hepatocytes, indicating applicability across a wide range of experimental systems and model organisms.
Published Workflows
Objective: Develop a reliable photo-activatable Gal4 transcription factor with robust light-induced gene expression and limited background activity in dark conditions for mammalian and other vertebrate experimental systems.
Why it works: The workflow is based on optimizing component configurations of a synthetic PA-Gal4 transcription factor so that the light-responsive VVD module supports stronger induced transcription while reducing dark-state activity, followed by validation across multiple delivery modes and biological systems.
blue light-dependent dimer formation via VVDconfiguration optimization of Gal4 DNA-binding domain and transcription activation domainsynthetic transcription factor optimizationcomparative validation in cultured cells and in vivo or ex vivo systems
Stages
- 1.Optimization of synthetic PA-Gal4 transcription factor configurations(library_design)
This stage exists to identify an improved PA-Gal4 configuration because prior PA-Gal4 transcription factors showed undesired background activity in dark conditions.
Selection: Configurations of Gal4 DNA-binding domain, transcription activation domain, and VVD-based light-dependent dimer formation were evaluated to develop an improved PA-Gal4 factor.
- 2.Cultured-cell verification of light-controlled gene expression(confirmatory_validation)
This stage confirms that the optimized factor functions in cultured mammalian cells before broader application claims.
Selection: Verification of light-controlled gene expression in cultured HEK293T and mouse EpH4 cells using different delivery modes.
- 3.Extended validation across neural and hepatic in vivo or ex vivo contexts(in_vivo_validation)
This stage tests whether the optimized transcription factor remains useful beyond cultured cell lines in diverse vertebrate biological contexts.
Selection: Confirmation of eGAV-mediated transcription in neural stem cells and progenitors in mouse brain and chick spinal cord, and in adult mouse hepatocytes.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
HeterodimerizationTechniques
No technique tags yet.
Target processes
transcriptionInput: Light
Implementation Constraints
eGAV-mediated gene expression was verified after plasmid transient transfection in HEK293T cells and after lentiviral vector-mediated transduction in mouse EpH4 cells. The provided evidence identifies light as the input modality, but does not specify wavelength, dosing regimen, or cofactor requirements.
The supplied evidence does not provide quantitative performance metrics, illumination parameters, construct architecture, or direct comparative data beyond the current summary statement. Independent replication is not documented in the provided material.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
eGAV-mediated light-controlled gene expression was verified in cultured HEK293T cells by plasmid transient transfection and in mouse EpH4 cells by lentiviral vector-mediated transduction.
eGAV-mediated light-controlled gene expression was verified in cultured HEK293T cells by plasmid transient transfection and in mouse EpH4 cells by lentiviral vector-mediated transduction.
eGAV-mediated light-controlled gene expression was verified in cultured HEK293T cells by plasmid transient transfection and in mouse EpH4 cells by lentiviral vector-mediated transduction.
eGAV-mediated light-controlled gene expression was verified in cultured HEK293T cells by plasmid transient transfection and in mouse EpH4 cells by lentiviral vector-mediated transduction.
eGAV-mediated light-controlled gene expression was verified in cultured HEK293T cells by plasmid transient transfection and in mouse EpH4 cells by lentiviral vector-mediated transduction.
eGAV-mediated light-controlled gene expression was verified in cultured HEK293T cells by plasmid transient transfection and in mouse EpH4 cells by lentiviral vector-mediated transduction.
eGAV-mediated light-controlled gene expression was verified in cultured HEK293T cells by plasmid transient transfection and in mouse EpH4 cells by lentiviral vector-mediated transduction.
eGAV-mediated light-controlled gene expression was verified in cultured HEK293T cells by plasmid transient transfection and in mouse EpH4 cells by lentiviral vector-mediated transduction.
eGAV-mediated light-controlled transcription was confirmed in neural stem cells and progenitors in developing and adult mouse brain and chick spinal cord, and in adult mouse hepatocytes, indicating applicability across a wide range of experimental systems and model organisms.
eGAV-mediated light-controlled transcription was confirmed in neural stem cells and progenitors in developing and adult mouse brain and chick spinal cord, and in adult mouse hepatocytes, indicating applicability across a wide range of experimental systems and model organisms.
eGAV-mediated light-controlled transcription was confirmed in neural stem cells and progenitors in developing and adult mouse brain and chick spinal cord, and in adult mouse hepatocytes, indicating applicability across a wide range of experimental systems and model organisms.
eGAV-mediated light-controlled transcription was confirmed in neural stem cells and progenitors in developing and adult mouse brain and chick spinal cord, and in adult mouse hepatocytes, indicating applicability across a wide range of experimental systems and model organisms.
eGAV-mediated light-controlled transcription was confirmed in neural stem cells and progenitors in developing and adult mouse brain and chick spinal cord, and in adult mouse hepatocytes, indicating applicability across a wide range of experimental systems and model organisms.
eGAV-mediated light-controlled transcription was confirmed in neural stem cells and progenitors in developing and adult mouse brain and chick spinal cord, and in adult mouse hepatocytes, indicating applicability across a wide range of experimental systems and model organisms.
eGAV-mediated light-controlled transcription was confirmed in neural stem cells and progenitors in developing and adult mouse brain and chick spinal cord, and in adult mouse hepatocytes, indicating applicability across a wide range of experimental systems and model organisms.
eGAV-mediated light-controlled transcription was confirmed in neural stem cells and progenitors in developing and adult mouse brain and chick spinal cord, and in adult mouse hepatocytes, indicating applicability across a wide range of experimental systems and model organisms.
eGAV achieves improved maximum light-induced gene expression levels relative to hGAVPO.
eGAV achieves improved maximum light-induced gene expression levels relative to hGAVPO.
eGAV achieves improved maximum light-induced gene expression levels relative to hGAVPO.
eGAV achieves improved maximum light-induced gene expression levels relative to hGAVPO.
eGAV achieves improved maximum light-induced gene expression levels relative to hGAVPO.
eGAV achieves improved maximum light-induced gene expression levels relative to hGAVPO.
eGAV achieves improved maximum light-induced gene expression levels relative to hGAVPO.
eGAV achieves improved maximum light-induced gene expression levels relative to hGAVPO.
eGAV has significantly lower background transcription activity in dark conditions than hGAVPO.
eGAV has significantly lower background transcription activity in dark conditions than hGAVPO.
eGAV has significantly lower background transcription activity in dark conditions than hGAVPO.
eGAV has significantly lower background transcription activity in dark conditions than hGAVPO.
eGAV has significantly lower background transcription activity in dark conditions than hGAVPO.
eGAV has significantly lower background transcription activity in dark conditions than hGAVPO.
eGAV has significantly lower background transcription activity in dark conditions than hGAVPO.
eGAV has significantly lower background transcription activity in dark conditions than hGAVPO.
eGAV is a new optimized photo-activatable Gal4 transcription factor developed by optimizing Gal4 DNA-binding domain, transcription activation domain, and VVD-based light-dependent dimerization configurations.
eGAV is a new optimized photo-activatable Gal4 transcription factor developed by optimizing Gal4 DNA-binding domain, transcription activation domain, and VVD-based light-dependent dimerization configurations.
eGAV is a new optimized photo-activatable Gal4 transcription factor developed by optimizing Gal4 DNA-binding domain, transcription activation domain, and VVD-based light-dependent dimerization configurations.
eGAV is a new optimized photo-activatable Gal4 transcription factor developed by optimizing Gal4 DNA-binding domain, transcription activation domain, and VVD-based light-dependent dimerization configurations.
eGAV is a new optimized photo-activatable Gal4 transcription factor developed by optimizing Gal4 DNA-binding domain, transcription activation domain, and VVD-based light-dependent dimerization configurations.
eGAV is a new optimized photo-activatable Gal4 transcription factor developed by optimizing Gal4 DNA-binding domain, transcription activation domain, and VVD-based light-dependent dimerization configurations.
eGAV is a new optimized photo-activatable Gal4 transcription factor developed by optimizing Gal4 DNA-binding domain, transcription activation domain, and VVD-based light-dependent dimerization configurations.
eGAV is a new optimized photo-activatable Gal4 transcription factor developed by optimizing Gal4 DNA-binding domain, transcription activation domain, and VVD-based light-dependent dimerization configurations.
Approval Evidence
1 source5 linked approval claimsfirst-pass slug egav
we have named eGAV (enhanced Gal4-VVD transcription factor). Background activity of eGAV in dark conditions was significantly lower than that of hGAVPO ... and maximum light-induced gene expression levels were also improved.
Source:
application scopesupports
eGAV-mediated light-controlled gene expression was verified in cultured HEK293T cells by plasmid transient transfection and in mouse EpH4 cells by lentiviral vector-mediated transduction.
Source:
application scopesupports
eGAV-mediated light-controlled transcription was confirmed in neural stem cells and progenitors in developing and adult mouse brain and chick spinal cord, and in adult mouse hepatocytes, indicating applicability across a wide range of experimental systems and model organisms.
Source:
comparative performancesupports
eGAV achieves improved maximum light-induced gene expression levels relative to hGAVPO.
Source:
comparative performancesupports
eGAV has significantly lower background transcription activity in dark conditions than hGAVPO.
Source:
tool performancesupports
eGAV is a new optimized photo-activatable Gal4 transcription factor developed by optimizing Gal4 DNA-binding domain, transcription activation domain, and VVD-based light-dependent dimerization configurations.
Source:
Comparisons
Source-backed strengths
The reported validation spans cultured HEK293T cells, mouse EpH4 cells, neural stem cells and progenitors in developing and adult mouse brain, chick spinal cord, and adult mouse hepatocytes. This breadth supports that eGAV can function in both in vitro and in vivo vertebrate settings.
Source:
eGAV achieves improved maximum light-induced gene expression levels relative to hGAVPO.
Source:
eGAV has significantly lower background transcription activity in dark conditions than hGAVPO.
Ranked Citations
- 1.