Source 1primary paper2023Scientific Reports
Toolkit/EGxxFP Cas9 reporter
EGxxFP Cas9 reporter
Also known as: Cas9 reporter (EGxxFP), EGxxFP
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
EGxxFP is a Cas9 reporter construct used in split-Cas9 synthetic circuits to convert successful Cas9 reconstitution into a fluorescent readout. In the cited 2023 Scientific Reports study, it reported cellular states and events including cancer epithelial origin, epithelial-to-mesenchymal transition, and cell-cell fusion.
Usefulness & Problems
Why this is useful
This construct is useful as a fluorescence-based output module for synthetic circuits that sense combinatorial cellular inputs through split Cas9. The reported applications show that it can indicate specific biological events that are otherwise difficult to encode into a single genetically programmable reporter, including EMT-associated state changes and fusion-derived syncytia.
Source:
First, we positioned the complementing halves of split Cas9 under different promoters-one specific to cancer cells of epithelial origin (p hCEA) and the other a universal promoter... Consequently, only cancer cells with an epithelial origin activated the reporter, exhibiting green fluorescence.
Source:
we designed a logic gate where one half of Cas9 is expressed under the p hCEA, while the other is activated by TWIST1. The results showed that cells undergoing EMT effectively activated the reporter.
Source:
we combined the two inputs (epithelial origin and EMT) to create a new logic gate, where only cancer epithelial cells undergoing EMT activated the reporter
Source:
we applied the split-Cas9 logic gate as a sensor of cell-cell fusion, both in induced and naturally occurring scenarios. Each cell type expressed one half of split Cas9, and the induction of fusion resulted in the appearance of multinucleated syncytia and the fluorescent reporter.
Problem solved
EGxxFP helps solve the problem of linking conditional split Cas9 activity to an experimentally tractable reporter signal in mammalian cell-state sensing circuits. In the cited work, it enabled detection of epithelial cancer identity, EMT, and cell-cell fusion only when the appropriate split Cas9 logic conditions were met.
Problem links
Need conditional recombination or state switching
DerivedEGxxFP is a Cas9 reporter construct used in split-Cas9 synthetic circuits to convert successful Cas9 reconstitution into a fluorescent readout. In the cited 2023 Scientific Reports study, it reported cellular states and events including cancer epithelial origin, epithelial-to-mesenchymal transition, and cell-cell fusion.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Mechanisms
intein-mediated protein splicingreporter activation following cas9 reconstitutionsplit-protein reconstitutiontranscriptional logic gatingTechniques
Computational DesignTarget processes
recombinationImplementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensorswitch architecture: split
In the reported circuits, split Cas9 halves were reconstituted using self-assembling inteins, and reporter activation depended on successful reconstitution of Cas9 activity. Practical implementation in the cited study involved promoter-controlled expression of the split halves, including an epithelial cancer-specific promoter, p hCEA, a TWIST1-activated input, and a universal promoter, but the evidence does not provide further construct-design or delivery details for EGxxFP.
The available evidence is limited to a single cited study and does not provide detailed quantitative performance metrics for EGxxFP itself, such as dynamic range, background fluorescence, response kinetics, or sensitivity. The evidence also does not specify the exact molecular architecture of the reporter construct beyond its use as a Cas9 reporter in split-Cas9 circuits.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Observations
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c2 during normalization. A split Cas9 gate with one half under an epithelial cancer-specific promoter and the other under a universal promoter activated the reporter only in cancer cells with epithelial origin. Derived from claim c2. Quoted text: First, we positioned the complementing halves of split Cas9 under different promoters-one specific to cancer cells of epithelial origin (p hCEA) and the other a universal promoter... Consequently, only cancer cells with an epithelial origin activated the reporter, exhibiting green fluorescence.
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c3 during normalization. A split Cas9 gate with one half under p hCEA and the other activated by TWIST1 detected cells undergoing epithelial to mesenchymal transition by reporter activation. Derived from claim c3. Quoted text: we designed a logic gate where one half of Cas9 is expressed under the p hCEA, while the other is activated by TWIST1. The results showed that cells undergoing EMT effectively activated the reporter.
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c4 during normalization. Combining epithelial origin and EMT inputs produced a split Cas9 logic gate that activated the reporter only in cancer epithelial cells undergoing EMT. Derived from claim c4. Quoted text: we combined the two inputs (epithelial origin and EMT) to create a new logic gate, where only cancer epithelial cells undergoing EMT activated the reporter
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c5 during normalization. The split Cas9 logic gate functioned as a sensor of cell-cell fusion in induced and naturally occurring scenarios, with fusion producing multinucleated syncytia and reporter fluorescence. Derived from claim c5. Quoted text: we applied the split-Cas9 logic gate as a sensor of cell-cell fusion, both in induced and naturally occurring scenarios. Each cell type expressed one half of split Cas9, and the induction of fusion resulted in the appearance of multinucleated syncytia and the fluorescent reporter.
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c2 during normalization. A split Cas9 gate with one half under an epithelial cancer-specific promoter and the other under a universal promoter activated the reporter only in cancer cells with epithelial origin. Derived from claim c2. Quoted text: First, we positioned the complementing halves of split Cas9 under different promoters-one specific to cancer cells of epithelial origin (p hCEA) and the other a universal promoter... Consequently, only cancer cells with an epithelial origin activated the reporter, exhibiting green fluorescence.
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c3 during normalization. A split Cas9 gate with one half under p hCEA and the other activated by TWIST1 detected cells undergoing epithelial to mesenchymal transition by reporter activation. Derived from claim c3. Quoted text: we designed a logic gate where one half of Cas9 is expressed under the p hCEA, while the other is activated by TWIST1. The results showed that cells undergoing EMT effectively activated the reporter.
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c4 during normalization. Combining epithelial origin and EMT inputs produced a split Cas9 logic gate that activated the reporter only in cancer epithelial cells undergoing EMT. Derived from claim c4. Quoted text: we combined the two inputs (epithelial origin and EMT) to create a new logic gate, where only cancer epithelial cells undergoing EMT activated the reporter
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c5 during normalization. The split Cas9 logic gate functioned as a sensor of cell-cell fusion in induced and naturally occurring scenarios, with fusion producing multinucleated syncytia and reporter fluorescence. Derived from claim c5. Quoted text: we applied the split-Cas9 logic gate as a sensor of cell-cell fusion, both in induced and naturally occurring scenarios. Each cell type expressed one half of split Cas9, and the induction of fusion resulted in the appearance of multinucleated syncytia and the fluorescent reporter.
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c2 during normalization. A split Cas9 gate with one half under an epithelial cancer-specific promoter and the other under a universal promoter activated the reporter only in cancer cells with epithelial origin. Derived from claim c2. Quoted text: First, we positioned the complementing halves of split Cas9 under different promoters-one specific to cancer cells of epithelial origin (p hCEA) and the other a universal promoter... Consequently, only cancer cells with an epithelial origin activated the reporter, exhibiting green fluorescence.
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c3 during normalization. A split Cas9 gate with one half under p hCEA and the other activated by TWIST1 detected cells undergoing epithelial to mesenchymal transition by reporter activation. Derived from claim c3. Quoted text: we designed a logic gate where one half of Cas9 is expressed under the p hCEA, while the other is activated by TWIST1. The results showed that cells undergoing EMT effectively activated the reporter.
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c4 during normalization. Combining epithelial origin and EMT inputs produced a split Cas9 logic gate that activated the reporter only in cancer epithelial cells undergoing EMT. Derived from claim c4. Quoted text: we combined the two inputs (epithelial origin and EMT) to create a new logic gate, where only cancer epithelial cells undergoing EMT activated the reporter
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c5 during normalization. The split Cas9 logic gate functioned as a sensor of cell-cell fusion in induced and naturally occurring scenarios, with fusion producing multinucleated syncytia and reporter fluorescence. Derived from claim c5. Quoted text: we applied the split-Cas9 logic gate as a sensor of cell-cell fusion, both in induced and naturally occurring scenarios. Each cell type expressed one half of split Cas9, and the induction of fusion resulted in the appearance of multinucleated syncytia and the fluorescent reporter.
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c2 during normalization. A split Cas9 gate with one half under an epithelial cancer-specific promoter and the other under a universal promoter activated the reporter only in cancer cells with epithelial origin. Derived from claim c2. Quoted text: First, we positioned the complementing halves of split Cas9 under different promoters-one specific to cancer cells of epithelial origin (p hCEA) and the other a universal promoter... Consequently, only cancer cells with an epithelial origin activated the reporter, exhibiting green fluorescence.
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c3 during normalization. A split Cas9 gate with one half under p hCEA and the other activated by TWIST1 detected cells undergoing epithelial to mesenchymal transition by reporter activation. Derived from claim c3. Quoted text: we designed a logic gate where one half of Cas9 is expressed under the p hCEA, while the other is activated by TWIST1. The results showed that cells undergoing EMT effectively activated the reporter.
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c4 during normalization. Combining epithelial origin and EMT inputs produced a split Cas9 logic gate that activated the reporter only in cancer epithelial cells undergoing EMT. Derived from claim c4. Quoted text: we combined the two inputs (epithelial origin and EMT) to create a new logic gate, where only cancer epithelial cells undergoing EMT activated the reporter
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c5 during normalization. The split Cas9 logic gate functioned as a sensor of cell-cell fusion in induced and naturally occurring scenarios, with fusion producing multinucleated syncytia and reporter fluorescence. Derived from claim c5. Quoted text: we applied the split-Cas9 logic gate as a sensor of cell-cell fusion, both in induced and naturally occurring scenarios. Each cell type expressed one half of split Cas9, and the induction of fusion resulted in the appearance of multinucleated syncytia and the fluorescent reporter.
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c2 during normalization. A split Cas9 gate with one half under an epithelial cancer-specific promoter and the other under a universal promoter activated the reporter only in cancer cells with epithelial origin. Derived from claim c2. Quoted text: First, we positioned the complementing halves of split Cas9 under different promoters-one specific to cancer cells of epithelial origin (p hCEA) and the other a universal promoter... Consequently, only cancer cells with an epithelial origin activated the reporter, exhibiting green fluorescence.
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c3 during normalization. A split Cas9 gate with one half under p hCEA and the other activated by TWIST1 detected cells undergoing epithelial to mesenchymal transition by reporter activation. Derived from claim c3. Quoted text: we designed a logic gate where one half of Cas9 is expressed under the p hCEA, while the other is activated by TWIST1. The results showed that cells undergoing EMT effectively activated the reporter.
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c4 during normalization. Combining epithelial origin and EMT inputs produced a split Cas9 logic gate that activated the reporter only in cancer epithelial cells undergoing EMT. Derived from claim c4. Quoted text: we combined the two inputs (epithelial origin and EMT) to create a new logic gate, where only cancer epithelial cells undergoing EMT activated the reporter
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c5 during normalization. The split Cas9 logic gate functioned as a sensor of cell-cell fusion in induced and naturally occurring scenarios, with fusion producing multinucleated syncytia and reporter fluorescence. Derived from claim c5. Quoted text: we applied the split-Cas9 logic gate as a sensor of cell-cell fusion, both in induced and naturally occurring scenarios. Each cell type expressed one half of split Cas9, and the induction of fusion resulted in the appearance of multinucleated syncytia and the fluorescent reporter.
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c2 during normalization. A split Cas9 gate with one half under an epithelial cancer-specific promoter and the other under a universal promoter activated the reporter only in cancer cells with epithelial origin. Derived from claim c2. Quoted text: First, we positioned the complementing halves of split Cas9 under different promoters-one specific to cancer cells of epithelial origin (p hCEA) and the other a universal promoter... Consequently, only cancer cells with an epithelial origin activated the reporter, exhibiting green fluorescence.
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c3 during normalization. A split Cas9 gate with one half under p hCEA and the other activated by TWIST1 detected cells undergoing epithelial to mesenchymal transition by reporter activation. Derived from claim c3. Quoted text: we designed a logic gate where one half of Cas9 is expressed under the p hCEA, while the other is activated by TWIST1. The results showed that cells undergoing EMT effectively activated the reporter.
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c4 during normalization. Combining epithelial origin and EMT inputs produced a split Cas9 logic gate that activated the reporter only in cancer epithelial cells undergoing EMT. Derived from claim c4. Quoted text: we combined the two inputs (epithelial origin and EMT) to create a new logic gate, where only cancer epithelial cells undergoing EMT activated the reporter
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c5 during normalization. The split Cas9 logic gate functioned as a sensor of cell-cell fusion in induced and naturally occurring scenarios, with fusion producing multinucleated syncytia and reporter fluorescence. Derived from claim c5. Quoted text: we applied the split-Cas9 logic gate as a sensor of cell-cell fusion, both in induced and naturally occurring scenarios. Each cell type expressed one half of split Cas9, and the induction of fusion resulted in the appearance of multinucleated syncytia and the fluorescent reporter.
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c2 during normalization. A split Cas9 gate with one half under an epithelial cancer-specific promoter and the other under a universal promoter activated the reporter only in cancer cells with epithelial origin. Derived from claim c2. Quoted text: First, we positioned the complementing halves of split Cas9 under different promoters-one specific to cancer cells of epithelial origin (p hCEA) and the other a universal promoter... Consequently, only cancer cells with an epithelial origin activated the reporter, exhibiting green fluorescence.
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c3 during normalization. A split Cas9 gate with one half under p hCEA and the other activated by TWIST1 detected cells undergoing epithelial to mesenchymal transition by reporter activation. Derived from claim c3. Quoted text: we designed a logic gate where one half of Cas9 is expressed under the p hCEA, while the other is activated by TWIST1. The results showed that cells undergoing EMT effectively activated the reporter.
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c4 during normalization. Combining epithelial origin and EMT inputs produced a split Cas9 logic gate that activated the reporter only in cancer epithelial cells undergoing EMT. Derived from claim c4. Quoted text: we combined the two inputs (epithelial origin and EMT) to create a new logic gate, where only cancer epithelial cells undergoing EMT activated the reporter
Source:
successMammalian Cell Lineapplication demo
fluorescent reporter
Inferred from claim c5 during normalization. The split Cas9 logic gate functioned as a sensor of cell-cell fusion in induced and naturally occurring scenarios, with fusion producing multinucleated syncytia and reporter fluorescence. Derived from claim c5. Quoted text: we applied the split-Cas9 logic gate as a sensor of cell-cell fusion, both in induced and naturally occurring scenarios. Each cell type expressed one half of split Cas9, and the induction of fusion resulted in the appearance of multinucleated syncytia and the fluorescent reporter.
Source:
Supporting Sources
Ranked Claims
Self-assembling inteins facilitated reconstitution of the split Cas9 halves.
The use of self-assembling inteins facilitated the reconstitution of the Cas9 halves.
Self-assembling inteins facilitated reconstitution of the split Cas9 halves.
The use of self-assembling inteins facilitated the reconstitution of the Cas9 halves.
Self-assembling inteins facilitated reconstitution of the split Cas9 halves.
The use of self-assembling inteins facilitated the reconstitution of the Cas9 halves.
Self-assembling inteins facilitated reconstitution of the split Cas9 halves.
The use of self-assembling inteins facilitated the reconstitution of the Cas9 halves.
Self-assembling inteins facilitated reconstitution of the split Cas9 halves.
The use of self-assembling inteins facilitated the reconstitution of the Cas9 halves.
Self-assembling inteins facilitated reconstitution of the split Cas9 halves.
The use of self-assembling inteins facilitated the reconstitution of the Cas9 halves.
Self-assembling inteins facilitated reconstitution of the split Cas9 halves.
The use of self-assembling inteins facilitated the reconstitution of the Cas9 halves.
A split Cas9 gate with one half under an epithelial cancer-specific promoter and the other under a universal promoter activated the reporter only in cancer cells with epithelial origin.
First, we positioned the complementing halves of split Cas9 under different promoters-one specific to cancer cells of epithelial origin (p hCEA) and the other a universal promoter... Consequently, only cancer cells with an epithelial origin activated the reporter, exhibiting green fluorescence.
A split Cas9 gate with one half under an epithelial cancer-specific promoter and the other under a universal promoter activated the reporter only in cancer cells with epithelial origin.
First, we positioned the complementing halves of split Cas9 under different promoters-one specific to cancer cells of epithelial origin (p hCEA) and the other a universal promoter... Consequently, only cancer cells with an epithelial origin activated the reporter, exhibiting green fluorescence.
A split Cas9 gate with one half under an epithelial cancer-specific promoter and the other under a universal promoter activated the reporter only in cancer cells with epithelial origin.
First, we positioned the complementing halves of split Cas9 under different promoters-one specific to cancer cells of epithelial origin (p hCEA) and the other a universal promoter... Consequently, only cancer cells with an epithelial origin activated the reporter, exhibiting green fluorescence.
A split Cas9 gate with one half under an epithelial cancer-specific promoter and the other under a universal promoter activated the reporter only in cancer cells with epithelial origin.
First, we positioned the complementing halves of split Cas9 under different promoters-one specific to cancer cells of epithelial origin (p hCEA) and the other a universal promoter... Consequently, only cancer cells with an epithelial origin activated the reporter, exhibiting green fluorescence.
A split Cas9 gate with one half under an epithelial cancer-specific promoter and the other under a universal promoter activated the reporter only in cancer cells with epithelial origin.
First, we positioned the complementing halves of split Cas9 under different promoters-one specific to cancer cells of epithelial origin (p hCEA) and the other a universal promoter... Consequently, only cancer cells with an epithelial origin activated the reporter, exhibiting green fluorescence.
A split Cas9 gate with one half under an epithelial cancer-specific promoter and the other under a universal promoter activated the reporter only in cancer cells with epithelial origin.
First, we positioned the complementing halves of split Cas9 under different promoters-one specific to cancer cells of epithelial origin (p hCEA) and the other a universal promoter... Consequently, only cancer cells with an epithelial origin activated the reporter, exhibiting green fluorescence.
A split Cas9 gate with one half under an epithelial cancer-specific promoter and the other under a universal promoter activated the reporter only in cancer cells with epithelial origin.
First, we positioned the complementing halves of split Cas9 under different promoters-one specific to cancer cells of epithelial origin (p hCEA) and the other a universal promoter... Consequently, only cancer cells with an epithelial origin activated the reporter, exhibiting green fluorescence.
A split Cas9 gate with one half under p hCEA and the other activated by TWIST1 detected cells undergoing epithelial to mesenchymal transition by reporter activation.
we designed a logic gate where one half of Cas9 is expressed under the p hCEA, while the other is activated by TWIST1. The results showed that cells undergoing EMT effectively activated the reporter.
A split Cas9 gate with one half under p hCEA and the other activated by TWIST1 detected cells undergoing epithelial to mesenchymal transition by reporter activation.
we designed a logic gate where one half of Cas9 is expressed under the p hCEA, while the other is activated by TWIST1. The results showed that cells undergoing EMT effectively activated the reporter.
A split Cas9 gate with one half under p hCEA and the other activated by TWIST1 detected cells undergoing epithelial to mesenchymal transition by reporter activation.
we designed a logic gate where one half of Cas9 is expressed under the p hCEA, while the other is activated by TWIST1. The results showed that cells undergoing EMT effectively activated the reporter.
A split Cas9 gate with one half under p hCEA and the other activated by TWIST1 detected cells undergoing epithelial to mesenchymal transition by reporter activation.
we designed a logic gate where one half of Cas9 is expressed under the p hCEA, while the other is activated by TWIST1. The results showed that cells undergoing EMT effectively activated the reporter.
A split Cas9 gate with one half under p hCEA and the other activated by TWIST1 detected cells undergoing epithelial to mesenchymal transition by reporter activation.
we designed a logic gate where one half of Cas9 is expressed under the p hCEA, while the other is activated by TWIST1. The results showed that cells undergoing EMT effectively activated the reporter.
A split Cas9 gate with one half under p hCEA and the other activated by TWIST1 detected cells undergoing epithelial to mesenchymal transition by reporter activation.
we designed a logic gate where one half of Cas9 is expressed under the p hCEA, while the other is activated by TWIST1. The results showed that cells undergoing EMT effectively activated the reporter.
A split Cas9 gate with one half under p hCEA and the other activated by TWIST1 detected cells undergoing epithelial to mesenchymal transition by reporter activation.
we designed a logic gate where one half of Cas9 is expressed under the p hCEA, while the other is activated by TWIST1. The results showed that cells undergoing EMT effectively activated the reporter.
Combining epithelial origin and EMT inputs produced a split Cas9 logic gate that activated the reporter only in cancer epithelial cells undergoing EMT.
we combined the two inputs (epithelial origin and EMT) to create a new logic gate, where only cancer epithelial cells undergoing EMT activated the reporter
Combining epithelial origin and EMT inputs produced a split Cas9 logic gate that activated the reporter only in cancer epithelial cells undergoing EMT.
we combined the two inputs (epithelial origin and EMT) to create a new logic gate, where only cancer epithelial cells undergoing EMT activated the reporter
Combining epithelial origin and EMT inputs produced a split Cas9 logic gate that activated the reporter only in cancer epithelial cells undergoing EMT.
we combined the two inputs (epithelial origin and EMT) to create a new logic gate, where only cancer epithelial cells undergoing EMT activated the reporter
Combining epithelial origin and EMT inputs produced a split Cas9 logic gate that activated the reporter only in cancer epithelial cells undergoing EMT.
we combined the two inputs (epithelial origin and EMT) to create a new logic gate, where only cancer epithelial cells undergoing EMT activated the reporter
Combining epithelial origin and EMT inputs produced a split Cas9 logic gate that activated the reporter only in cancer epithelial cells undergoing EMT.
we combined the two inputs (epithelial origin and EMT) to create a new logic gate, where only cancer epithelial cells undergoing EMT activated the reporter
Combining epithelial origin and EMT inputs produced a split Cas9 logic gate that activated the reporter only in cancer epithelial cells undergoing EMT.
we combined the two inputs (epithelial origin and EMT) to create a new logic gate, where only cancer epithelial cells undergoing EMT activated the reporter
Combining epithelial origin and EMT inputs produced a split Cas9 logic gate that activated the reporter only in cancer epithelial cells undergoing EMT.
we combined the two inputs (epithelial origin and EMT) to create a new logic gate, where only cancer epithelial cells undergoing EMT activated the reporter
The split Cas9 logic gate functioned as a sensor of cell-cell fusion in induced and naturally occurring scenarios, with fusion producing multinucleated syncytia and reporter fluorescence.
we applied the split-Cas9 logic gate as a sensor of cell-cell fusion, both in induced and naturally occurring scenarios. Each cell type expressed one half of split Cas9, and the induction of fusion resulted in the appearance of multinucleated syncytia and the fluorescent reporter.
The split Cas9 logic gate functioned as a sensor of cell-cell fusion in induced and naturally occurring scenarios, with fusion producing multinucleated syncytia and reporter fluorescence.
we applied the split-Cas9 logic gate as a sensor of cell-cell fusion, both in induced and naturally occurring scenarios. Each cell type expressed one half of split Cas9, and the induction of fusion resulted in the appearance of multinucleated syncytia and the fluorescent reporter.
The split Cas9 logic gate functioned as a sensor of cell-cell fusion in induced and naturally occurring scenarios, with fusion producing multinucleated syncytia and reporter fluorescence.
we applied the split-Cas9 logic gate as a sensor of cell-cell fusion, both in induced and naturally occurring scenarios. Each cell type expressed one half of split Cas9, and the induction of fusion resulted in the appearance of multinucleated syncytia and the fluorescent reporter.
The split Cas9 logic gate functioned as a sensor of cell-cell fusion in induced and naturally occurring scenarios, with fusion producing multinucleated syncytia and reporter fluorescence.
we applied the split-Cas9 logic gate as a sensor of cell-cell fusion, both in induced and naturally occurring scenarios. Each cell type expressed one half of split Cas9, and the induction of fusion resulted in the appearance of multinucleated syncytia and the fluorescent reporter.
The split Cas9 logic gate functioned as a sensor of cell-cell fusion in induced and naturally occurring scenarios, with fusion producing multinucleated syncytia and reporter fluorescence.
we applied the split-Cas9 logic gate as a sensor of cell-cell fusion, both in induced and naturally occurring scenarios. Each cell type expressed one half of split Cas9, and the induction of fusion resulted in the appearance of multinucleated syncytia and the fluorescent reporter.
The split Cas9 logic gate functioned as a sensor of cell-cell fusion in induced and naturally occurring scenarios, with fusion producing multinucleated syncytia and reporter fluorescence.
we applied the split-Cas9 logic gate as a sensor of cell-cell fusion, both in induced and naturally occurring scenarios. Each cell type expressed one half of split Cas9, and the induction of fusion resulted in the appearance of multinucleated syncytia and the fluorescent reporter.
The split Cas9 logic gate functioned as a sensor of cell-cell fusion in induced and naturally occurring scenarios, with fusion producing multinucleated syncytia and reporter fluorescence.
we applied the split-Cas9 logic gate as a sensor of cell-cell fusion, both in induced and naturally occurring scenarios. Each cell type expressed one half of split Cas9, and the induction of fusion resulted in the appearance of multinucleated syncytia and the fluorescent reporter.
An adaptation of split Cas9 can generate logic gates that sense biological events.
we present an adaptation of split Cas9 to generate logic gates capable of sensing biological events
An adaptation of split Cas9 can generate logic gates that sense biological events.
we present an adaptation of split Cas9 to generate logic gates capable of sensing biological events
An adaptation of split Cas9 can generate logic gates that sense biological events.
we present an adaptation of split Cas9 to generate logic gates capable of sensing biological events
An adaptation of split Cas9 can generate logic gates that sense biological events.
we present an adaptation of split Cas9 to generate logic gates capable of sensing biological events
An adaptation of split Cas9 can generate logic gates that sense biological events.
we present an adaptation of split Cas9 to generate logic gates capable of sensing biological events
An adaptation of split Cas9 can generate logic gates that sense biological events.
we present an adaptation of split Cas9 to generate logic gates capable of sensing biological events
An adaptation of split Cas9 can generate logic gates that sense biological events.
we present an adaptation of split Cas9 to generate logic gates capable of sensing biological events
The split Cas9 system is presented as simple and potentially integrable into various cellular processes as both a sensor and an actuator.
The simplicity of the split Cas9 system presented here allows for its integration into various cellular processes, not only as a sensor but also as an actuator.
The split Cas9 system is presented as simple and potentially integrable into various cellular processes as both a sensor and an actuator.
The simplicity of the split Cas9 system presented here allows for its integration into various cellular processes, not only as a sensor but also as an actuator.
The split Cas9 system is presented as simple and potentially integrable into various cellular processes as both a sensor and an actuator.
The simplicity of the split Cas9 system presented here allows for its integration into various cellular processes, not only as a sensor but also as an actuator.
The split Cas9 system is presented as simple and potentially integrable into various cellular processes as both a sensor and an actuator.
The simplicity of the split Cas9 system presented here allows for its integration into various cellular processes, not only as a sensor but also as an actuator.
The split Cas9 system is presented as simple and potentially integrable into various cellular processes as both a sensor and an actuator.
The simplicity of the split Cas9 system presented here allows for its integration into various cellular processes, not only as a sensor but also as an actuator.
The split Cas9 system is presented as simple and potentially integrable into various cellular processes as both a sensor and an actuator.
The simplicity of the split Cas9 system presented here allows for its integration into various cellular processes, not only as a sensor but also as an actuator.
The split Cas9 system is presented as simple and potentially integrable into various cellular processes as both a sensor and an actuator.
The simplicity of the split Cas9 system presented here allows for its integration into various cellular processes, not only as a sensor but also as an actuator.
Approval Evidence
1 source4 linked approval claimsfirst-pass slug egxxfp-cas9-reporter
leveraging a Cas9 reporter (EGxxFP) to detect occurrences such as cancer cell origin, epithelial to mesenchymal transition (EMT), and cell-cell fusion
Source:
functional resultsupports
A split Cas9 gate with one half under an epithelial cancer-specific promoter and the other under a universal promoter activated the reporter only in cancer cells with epithelial origin.
First, we positioned the complementing halves of split Cas9 under different promoters-one specific to cancer cells of epithelial origin (p hCEA) and the other a universal promoter... Consequently, only cancer cells with an epithelial origin activated the reporter, exhibiting green fluorescence.
Source:
functional resultsupports
A split Cas9 gate with one half under p hCEA and the other activated by TWIST1 detected cells undergoing epithelial to mesenchymal transition by reporter activation.
we designed a logic gate where one half of Cas9 is expressed under the p hCEA, while the other is activated by TWIST1. The results showed that cells undergoing EMT effectively activated the reporter.
Source:
functional resultsupports
Combining epithelial origin and EMT inputs produced a split Cas9 logic gate that activated the reporter only in cancer epithelial cells undergoing EMT.
we combined the two inputs (epithelial origin and EMT) to create a new logic gate, where only cancer epithelial cells undergoing EMT activated the reporter
Source:
functional resultsupports
The split Cas9 logic gate functioned as a sensor of cell-cell fusion in induced and naturally occurring scenarios, with fusion producing multinucleated syncytia and reporter fluorescence.
we applied the split-Cas9 logic gate as a sensor of cell-cell fusion, both in induced and naturally occurring scenarios. Each cell type expressed one half of split Cas9, and the induction of fusion resulted in the appearance of multinucleated syncytia and the fluorescent reporter.
Source:
Comparisons
Source-backed strengths
The reporter was validated in multiple split Cas9 circuit contexts, including gates driven by an epithelial cancer-specific promoter, by p hCEA with a TWIST1-responsive input, and by fusion-dependent complementation. The study reported selective reporter activation in cancer cells with epithelial origin, in cancer epithelial cells undergoing EMT, and in induced and naturally occurring cell-cell fusion events that produced multinucleated syncytia.
EGxxFP Cas9 reporter and cell-specific receptor subtype gene deletion mouse models address a similar problem space because they share recombination.
Shared frame: same top-level item type; shared target processes: recombination
Strengths here: looks easier to implement in practice.
Compared with eNpHR
EGxxFP Cas9 reporter and eNpHR address a similar problem space because they share recombination.
Shared frame: same top-level item type; shared target processes: recombination
Strengths here: looks easier to implement in practice; may avoid an exogenous cofactor requirement.
Compared with katA::mCherry replacement construct
EGxxFP Cas9 reporter and katA::mCherry replacement construct address a similar problem space because they share recombination.
Shared frame: same top-level item type; shared target processes: recombination
Ranked Citations
- 1.