Source 1primary paper2023
Toolkit/EL222 and LOVdeg system
EL222 and LOVdeg system
Also known as: combined EL222 and LOVdeg system
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The combined EL222 and LOVdeg system is a light-responsive multi-component optogenetic system developed in Escherichia coli by pairing the existing EL222 module with the LOVdeg blue-light-inducible degradation tag. It is intended to enhance optogenetic performance by combining EL222-based control with post-translational light-inducible protein degradation.
Usefulness & Problems
Why this is useful
This system is useful because it combines an existing optogenetic module with a degradative light-control layer, enabling post-translational regulation in addition to EL222-based optogenetic control. The source specifically states that pairing LOVdeg with existing optogenetic tools was used to enhance performance.
Source:
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
Source:
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
Source:
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
Problem solved
The system addresses the problem of improving the performance of an existing bacterial optogenetic tool by adding light-inducible protein degradation. More specific performance metrics or target behaviors for the combined EL222 and LOVdeg configuration were not provided in the supplied evidence.
Source:
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
Source:
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
Problem links
Need precise spatiotemporal control with light input
DerivedThe combined EL222 and LOVdeg system is a light-responsive multi-component bacterial optogenetic system that pairs the EL222 optogenetic module with the LOVdeg blue-light-inducible degradation tag. It was developed in Escherichia coli to enhance performance by combining an existing optogenetic tool with post-translational light-inducible protein degradation.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
light-inducible protein degradationlight-inducible protein degradationpost-translational controlpost-translational controlTechniques
No technique tags yet.
Target processes
No target processes tagged yet.
Input: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: multi component delivery burdenimplementation constraint: spectral hardware requirementoperating role: actuatorswitch architecture: multi component
The combined system was developed in Escherichia coli and relies on the LOVdeg tag for blue-light-inducible degradation of a protein of interest. The evidence supports modular combination of LOVdeg with an existing optogenetic tool, but construct architecture, illumination parameters, and EL222-specific design details were not provided.
The supplied evidence does not report quantitative characterization, dynamic range, kinetics, leakiness, or burden for the combined EL222 and LOVdeg system. Independent replication and validation outside the source study were not provided.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Source 2primary paper2024
Ranked Claims
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
The authors engineered LOVdeg, a tag for blue-light-inducible degradation of a protein of interest in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag for blue-light-inducible degradation of a protein of interest in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag for blue-light-inducible degradation of a protein of interest in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag for blue-light-inducible degradation of a protein of interest in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag for blue-light-inducible degradation of a protein of interest in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag for blue-light-inducible degradation of a protein of interest in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag for blue-light-inducible degradation of a protein of interest in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag for blue-light-inducible degradation of a protein of interest in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag for blue-light-inducible degradation of a protein of interest in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag for blue-light-inducible degradation of a protein of interest in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
LOVdeg is modular and was used to tag multiple proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular and was used to tag multiple proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular and was used to tag multiple proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular and was used to tag multiple proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular and was used to tag multiple proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular and was used to tag multiple proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular and was used to tag multiple proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular and was used to tag multiple proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular and was used to tag multiple proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular and was used to tag multiple proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
The results highlight the modularity and functionality of the LOVdeg tag system and introduce a new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The results highlight the modularity and functionality of the LOVdeg tag system and introduce a new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The results highlight the modularity and functionality of the LOVdeg tag system and introduce a new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The results highlight the modularity and functionality of the LOVdeg tag system and introduce a new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The results highlight the modularity and functionality of the LOVdeg tag system and introduce a new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The results highlight the modularity and functionality of the LOVdeg tag system and introduce a new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The results highlight the modularity and functionality of the LOVdeg tag system and introduce a new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The results highlight the modularity and functionality of the LOVdeg tag system and introduce a new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The results highlight the modularity and functionality of the LOVdeg tag system and introduce a new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The results highlight the modularity and functionality of the LOVdeg tag system and introduce a new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
A combined EL222 and LOVdeg system was developed to enhance performance by pairing LOVdeg with an existing optogenetic tool.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
A combined EL222 and LOVdeg system was developed to enhance performance by pairing LOVdeg with an existing optogenetic tool.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
A combined EL222 and LOVdeg system was developed to enhance performance by pairing LOVdeg with an existing optogenetic tool.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
A combined EL222 and LOVdeg system was developed to enhance performance by pairing LOVdeg with an existing optogenetic tool.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
A combined EL222 and LOVdeg system was developed to enhance performance by pairing LOVdeg with an existing optogenetic tool.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
A combined EL222 and LOVdeg system was developed to enhance performance by pairing LOVdeg with an existing optogenetic tool.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
A combined EL222 and LOVdeg system was developed to enhance performance by pairing LOVdeg with an existing optogenetic tool.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
A combined EL222 and LOVdeg system was developed to enhance performance by pairing LOVdeg with an existing optogenetic tool.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
A combined EL222 and LOVdeg system was developed to enhance performance by pairing LOVdeg with an existing optogenetic tool.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
A combined EL222 and LOVdeg system was developed to enhance performance by pairing LOVdeg with an existing optogenetic tool.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
A combined EL222 and LOVdeg system was developed to enhance performance by pairing LOVdeg with an existing optogenetic tool.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
A combined EL222 and LOVdeg system was developed to enhance performance by pairing LOVdeg with an existing optogenetic tool.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
A combined EL222 and LOVdeg system was developed to enhance performance by pairing LOVdeg with an existing optogenetic tool.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
A combined EL222 and LOVdeg system was developed to enhance performance by pairing LOVdeg with an existing optogenetic tool.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
A combined EL222 and LOVdeg system was developed to enhance performance by pairing LOVdeg with an existing optogenetic tool.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
A combined EL222 and LOVdeg system was developed to enhance performance by pairing LOVdeg with an existing optogenetic tool.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
LOVdeg was used in a metabolic engineering application to demonstrate post-translational control of metabolism.
Finally, we use the LOVdeg tag in a metabolic engineering application to demonstrate post-translational control of metabolism.
The authors engineered LOVdeg, a tag that can be appended to a protein of interest for blue-light-inducible degradation in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag that can be appended to a protein of interest for blue-light-inducible degradation in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag that can be appended to a protein of interest for blue-light-inducible degradation in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag that can be appended to a protein of interest for blue-light-inducible degradation in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag that can be appended to a protein of interest for blue-light-inducible degradation in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag that can be appended to a protein of interest for blue-light-inducible degradation in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag that can be appended to a protein of interest for blue-light-inducible degradation in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag that can be appended to a protein of interest for blue-light-inducible degradation in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag that can be appended to a protein of interest for blue-light-inducible degradation in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
The authors engineered LOVdeg, a tag that can be appended to a protein of interest for blue-light-inducible degradation in Escherichia coli.
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
LOVdeg is modular across multiple tagged proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular across multiple tagged proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular across multiple tagged proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular across multiple tagged proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular across multiple tagged proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular across multiple tagged proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular across multiple tagged proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular across multiple tagged proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular across multiple tagged proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
LOVdeg is modular across multiple tagged proteins including LacI repressor, a CRISPRa activator, and the AcrB efflux pump.
We demonstrate the modularity of LOVdeg by using it to tag a range of proteins, including the LacI repressor, CRISPRa activator, and the AcrB efflux pump.
Pairing LOVdeg with EL222 enhanced performance in a combined EL222 and LOVdeg system.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
Pairing LOVdeg with EL222 enhanced performance in a combined EL222 and LOVdeg system.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
Pairing LOVdeg with EL222 enhanced performance in a combined EL222 and LOVdeg system.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
Pairing LOVdeg with EL222 enhanced performance in a combined EL222 and LOVdeg system.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
Pairing LOVdeg with EL222 enhanced performance in a combined EL222 and LOVdeg system.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
Pairing LOVdeg with EL222 enhanced performance in a combined EL222 and LOVdeg system.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
Pairing LOVdeg with EL222 enhanced performance in a combined EL222 and LOVdeg system.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
Pairing LOVdeg with EL222 enhanced performance in a combined EL222 and LOVdeg system.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
Pairing LOVdeg with EL222 enhanced performance in a combined EL222 and LOVdeg system.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
Pairing LOVdeg with EL222 enhanced performance in a combined EL222 and LOVdeg system.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
Pairing LOVdeg with EL222 enhanced performance in a combined EL222 and LOVdeg system.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
Pairing LOVdeg with EL222 enhanced performance in a combined EL222 and LOVdeg system.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
Pairing LOVdeg with EL222 enhanced performance in a combined EL222 and LOVdeg system.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
Pairing LOVdeg with EL222 enhanced performance in a combined EL222 and LOVdeg system.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
Pairing LOVdeg with EL222 enhanced performance in a combined EL222 and LOVdeg system.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
Pairing LOVdeg with EL222 enhanced performance in a combined EL222 and LOVdeg system.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
Pairing LOVdeg with EL222 enhanced performance in a combined EL222 and LOVdeg system.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
The LOVdeg tag system is presented as a powerful new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The LOVdeg tag system is presented as a powerful new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The LOVdeg tag system is presented as a powerful new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The LOVdeg tag system is presented as a powerful new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The LOVdeg tag system is presented as a powerful new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The LOVdeg tag system is presented as a powerful new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The LOVdeg tag system is presented as a powerful new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The LOVdeg tag system is presented as a powerful new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The LOVdeg tag system is presented as a powerful new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
The LOVdeg tag system is presented as a powerful new tool for bacterial optogenetics.
Together, our results highlight the modularity and functionality of the LOVdeg tag system, and introduce a powerful new tool for bacterial optogenetics.
Approval Evidence
2 sources2 linked approval claimsfirst-pass slug el222-and-lovdeg-system
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
Source:
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
Source:
performance enhancementsupports
A combined EL222 and LOVdeg system was developed to enhance performance by pairing LOVdeg with an existing optogenetic tool.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
Source:
performance enhancementsupports
Pairing LOVdeg with EL222 enhanced performance in a combined EL222 and LOVdeg system.
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
Source:
Comparisons
Source-backed strengths
A key strength is modularity: LOVdeg was engineered as a blue-light-inducible degradation tag in E. coli and was applied to multiple proteins, including LacI, a CRISPRa activator, and AcrB. The underlying LOVdeg component was also demonstrated in a metabolic engineering application for post-translational control of metabolism, supporting its functional utility in bacterial contexts.
Source:
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
Source:
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
Source:
Here, we engineered LOVdeg, a tag that can be appended to a protein of interest for inducible degradation in Escherichia coli using blue light.
Source:
Additionally, we demonstrate the utility of pairing the LOVdeg tag with existing optogenetic tools to enhance performance by developing a combined EL222 and LOVdeg system.
Compared with ArrayG
EL222 and LOVdeg system and ArrayG address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Strengths here: appears more independently replicated; looks easier to implement in practice.
Compared with GFP-PHR-caspase8/Flag-CIB1N-caspase8
EL222 and LOVdeg system and GFP-PHR-caspase8/Flag-CIB1N-caspase8 address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Strengths here: appears more independently replicated; looks easier to implement in practice.
Compared with light-inducible split Cre recombinase
EL222 and LOVdeg system and light-inducible split Cre recombinase address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: post-translational control; same primary input modality: light
Strengths here: appears more independently replicated; looks easier to implement in practice.
Ranked Citations
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