Source 1primary paper2025MED
Toolkit/engineered bacteriophytochrome heterodimeric PSMs
engineered bacteriophytochrome heterodimeric PSMs
Also known as: heterodimeric PSMs, monomeric bacteriophytochrome PSMs that form stable heterodimers
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Here, we generate monomeric bacteriophytochrome PSMs that form stable heterodimers once mixed by modifying two salt bridges at the dimerization interface of the Deinococcus radiodurans phytochrome (DrBphP).
Usefulness & Problems
No literature-backed usefulness or problem-fit explainer has been materialized for this record yet.
Published Workflows
Objective: Engineer bacteriophytochrome photosensory modules that form stable heterodimers and use them to control downstream signaling and gene expression with red light.
Why it works: The workflow is based on modifying two salt bridges at the DrBphP dimerization interface so that monomeric PSM variants form stable heterodimers once mixed, allowing light sensing by the PSM to be coupled to downstream output control.
heterodimerization through engineered dimerization-interface salt bridgeslight-regulated control of histidine kinase outputdifferential dependence of kinase versus phosphatase activity on dimerizationprotein interface engineeringapplication of engineered variants to a gene expression tool
Stages
- 1.Engineering heterodimer-forming monomeric PSM variants(library_design)
This stage creates the complementary photosensory modules needed for heterodimeric control rather than native homodimerization.
Selection: Modification of two salt bridges at the DrBphP dimerization interface to generate monomeric PSMs that form stable heterodimers once mixed.
- 2.Functional confirmation of red-light control over HK output(functional_characterization)
This stage tests whether the engineered heterodimeric PSMs retain useful signaling control over downstream HK modules.
Selection: Whether heterodimeric PSMs can control output histidine kinase module activity in response to red light.
- 3.Mechanistic characterization of dimerization dependence(secondary_characterization)
This stage clarifies which output activities depend on dimerization and therefore informs how heterodimeric designs may control signaling behavior.
Selection: Comparison of dimerization requirements for kinase activity of FixL versus phosphatase activity of DrBphP.
- 4.Application in a red light-regulated gene expression tool(confirmatory_validation)
This stage demonstrates that the engineered heterodimeric variants are useful beyond mechanistic assays and can be deployed in an application-oriented optogenetic context.
Selection: Whether the heterodimeric variants can exemplify combined control of cellular events using heterodimerization and light in an applied gene-expression context.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Techniques
No technique tags yet.
Target processes
signalingInput: Light
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Applying the heterodimeric variants to a red light-regulated gene expression tool demonstrates combined control of cellular events using both heterodimerization and light.
Engineered monomeric DrBphP-derived photosensory modules can be made to form stable heterodimers after mixing by modifying two salt bridges at the dimerization interface.
The engineered heterodimeric bacteriophytochrome PSMs can control output histidine kinase module activity in response to red light.
Approval Evidence
1 source3 linked approval claimsfirst-pass slug engineered-bacteriophytochrome-heterodimeric-psms
Here, we generate monomeric bacteriophytochrome PSMs that form stable heterodimers once mixed by modifying two salt bridges at the dimerization interface of the Deinococcus radiodurans phytochrome (DrBphP).
Source:
application demosupports
Applying the heterodimeric variants to a red light-regulated gene expression tool demonstrates combined control of cellular events using both heterodimerization and light.
Source:
engineering resultsupports
Engineered monomeric DrBphP-derived photosensory modules can be made to form stable heterodimers after mixing by modifying two salt bridges at the dimerization interface.
Source:
functional controlsupports
The engineered heterodimeric bacteriophytochrome PSMs can control output histidine kinase module activity in response to red light.
Source:
Comparisons
No literature-backed comparison notes have been materialized for this record yet.
Ranked Citations
- 1.