Source 1review2017American Journal of Physiology-Regulatory, Integrative and Comparative Physiology
Toolkit/engineered G protein-coupled receptors
engineered G protein-coupled receptors
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Engineered G protein-coupled receptors are bioengineered pharmacogenetic tools for selective, remote control of neuronal activity. The supplied evidence describes receptors that are activated by otherwise inert drug-like small molecules rather than native ligands.
Usefulness & Problems
Why this is useful
These receptors provide a chemogenetic or pharmacogenetic route to modulate neuronal activity without relying on endogenous receptor ligands. The cited review places them within toolboxes that have enabled advances in dissecting nervous system function and its influence on physiological processes in health and disease.
Source:
These novel toolboxes are enabling significant advances in deciphering how the nervous system works and its influence on various physiological processes in health and disease.
Source:
Identification and subsequent bioengineering of light-sensitive ion channels (e.g., channelrhodopsins, halorhodopsin, and archaerhodopsins) from the bacteria have made it possible to use light to artificially modulate neuronal activity, namely optogenetics.
Source:
Recent advance in genetics has also allowed development of novel pharmacological tools to selectively and remotely control neuronal activity using engineered G protein-coupled receptors, which can be activated by otherwise inert drug-like small molecules such as the designer receptors exclusively activated by designer drug, a form of chemogenetics.
Problem solved
They address the need for selective and remote control of neuronal activity using novel pharmacological tools. Specifically, the evidence supports solving the problem of activating engineered receptors with otherwise inert drug-like small molecules instead of native ligands.
Source:
These novel toolboxes are enabling significant advances in deciphering how the nervous system works and its influence on various physiological processes in health and disease.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
chemogenetic activation by otherwise inert drug-like small moleculeschemogenetic activation by small moleculesgpcr-mediated signalingTechniques
Computational DesignTarget processes
No target processes tagged yet.
Input: Light
Implementation Constraints
Implementation is described only at the level of using engineered G protein-coupled receptors as pharmacological tools to control neuronal activity. The sources do not provide construct architecture, expression system, delivery method, dosing strategy, or cofactor requirements.
The supplied evidence is limited to a high-level review description and does not specify receptor variants, G-protein coupling class, ligand identities, kinetics, reversibility, or in vivo performance metrics. Although the input modality is listed as light, the evidence for this tool supports chemogenetic small-molecule activation rather than light activation.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
These optogenetic and chemogenetic toolboxes have enabled advances in deciphering nervous system function and its influence on physiological processes in health and disease.
These novel toolboxes are enabling significant advances in deciphering how the nervous system works and its influence on various physiological processes in health and disease.
These optogenetic and chemogenetic toolboxes have enabled advances in deciphering nervous system function and its influence on physiological processes in health and disease.
These novel toolboxes are enabling significant advances in deciphering how the nervous system works and its influence on various physiological processes in health and disease.
These optogenetic and chemogenetic toolboxes have enabled advances in deciphering nervous system function and its influence on physiological processes in health and disease.
These novel toolboxes are enabling significant advances in deciphering how the nervous system works and its influence on various physiological processes in health and disease.
These optogenetic and chemogenetic toolboxes have enabled advances in deciphering nervous system function and its influence on physiological processes in health and disease.
These novel toolboxes are enabling significant advances in deciphering how the nervous system works and its influence on various physiological processes in health and disease.
These optogenetic and chemogenetic toolboxes have enabled advances in deciphering nervous system function and its influence on physiological processes in health and disease.
These novel toolboxes are enabling significant advances in deciphering how the nervous system works and its influence on various physiological processes in health and disease.
These optogenetic and chemogenetic toolboxes have enabled advances in deciphering nervous system function and its influence on physiological processes in health and disease.
These novel toolboxes are enabling significant advances in deciphering how the nervous system works and its influence on various physiological processes in health and disease.
These optogenetic and chemogenetic toolboxes have enabled advances in deciphering nervous system function and its influence on physiological processes in health and disease.
These novel toolboxes are enabling significant advances in deciphering how the nervous system works and its influence on various physiological processes in health and disease.
Bioengineered light-sensitive ion channels including channelrhodopsins, halorhodopsin, and archaerhodopsins enable light-based artificial modulation of neuronal activity in optogenetics.
Identification and subsequent bioengineering of light-sensitive ion channels (e.g., channelrhodopsins, halorhodopsin, and archaerhodopsins) from the bacteria have made it possible to use light to artificially modulate neuronal activity, namely optogenetics.
Bioengineered light-sensitive ion channels including channelrhodopsins, halorhodopsin, and archaerhodopsins enable light-based artificial modulation of neuronal activity in optogenetics.
Identification and subsequent bioengineering of light-sensitive ion channels (e.g., channelrhodopsins, halorhodopsin, and archaerhodopsins) from the bacteria have made it possible to use light to artificially modulate neuronal activity, namely optogenetics.
Bioengineered light-sensitive ion channels including channelrhodopsins, halorhodopsin, and archaerhodopsins enable light-based artificial modulation of neuronal activity in optogenetics.
Identification and subsequent bioengineering of light-sensitive ion channels (e.g., channelrhodopsins, halorhodopsin, and archaerhodopsins) from the bacteria have made it possible to use light to artificially modulate neuronal activity, namely optogenetics.
Bioengineered light-sensitive ion channels including channelrhodopsins, halorhodopsin, and archaerhodopsins enable light-based artificial modulation of neuronal activity in optogenetics.
Identification and subsequent bioengineering of light-sensitive ion channels (e.g., channelrhodopsins, halorhodopsin, and archaerhodopsins) from the bacteria have made it possible to use light to artificially modulate neuronal activity, namely optogenetics.
Bioengineered light-sensitive ion channels including channelrhodopsins, halorhodopsin, and archaerhodopsins enable light-based artificial modulation of neuronal activity in optogenetics.
Identification and subsequent bioengineering of light-sensitive ion channels (e.g., channelrhodopsins, halorhodopsin, and archaerhodopsins) from the bacteria have made it possible to use light to artificially modulate neuronal activity, namely optogenetics.
Bioengineered light-sensitive ion channels including channelrhodopsins, halorhodopsin, and archaerhodopsins enable light-based artificial modulation of neuronal activity in optogenetics.
Identification and subsequent bioengineering of light-sensitive ion channels (e.g., channelrhodopsins, halorhodopsin, and archaerhodopsins) from the bacteria have made it possible to use light to artificially modulate neuronal activity, namely optogenetics.
Bioengineered light-sensitive ion channels including channelrhodopsins, halorhodopsin, and archaerhodopsins enable light-based artificial modulation of neuronal activity in optogenetics.
Identification and subsequent bioengineering of light-sensitive ion channels (e.g., channelrhodopsins, halorhodopsin, and archaerhodopsins) from the bacteria have made it possible to use light to artificially modulate neuronal activity, namely optogenetics.
Engineered G protein-coupled receptors activated by otherwise inert drug-like small molecules provide a chemogenetic or pharmacogenetic approach for selective remote control of neuronal activity.
Recent advance in genetics has also allowed development of novel pharmacological tools to selectively and remotely control neuronal activity using engineered G protein-coupled receptors, which can be activated by otherwise inert drug-like small molecules such as the designer receptors exclusively activated by designer drug, a form of chemogenetics.
Engineered G protein-coupled receptors activated by otherwise inert drug-like small molecules provide a chemogenetic or pharmacogenetic approach for selective remote control of neuronal activity.
Recent advance in genetics has also allowed development of novel pharmacological tools to selectively and remotely control neuronal activity using engineered G protein-coupled receptors, which can be activated by otherwise inert drug-like small molecules such as the designer receptors exclusively activated by designer drug, a form of chemogenetics.
Engineered G protein-coupled receptors activated by otherwise inert drug-like small molecules provide a chemogenetic or pharmacogenetic approach for selective remote control of neuronal activity.
Recent advance in genetics has also allowed development of novel pharmacological tools to selectively and remotely control neuronal activity using engineered G protein-coupled receptors, which can be activated by otherwise inert drug-like small molecules such as the designer receptors exclusively activated by designer drug, a form of chemogenetics.
Engineered G protein-coupled receptors activated by otherwise inert drug-like small molecules provide a chemogenetic or pharmacogenetic approach for selective remote control of neuronal activity.
Recent advance in genetics has also allowed development of novel pharmacological tools to selectively and remotely control neuronal activity using engineered G protein-coupled receptors, which can be activated by otherwise inert drug-like small molecules such as the designer receptors exclusively activated by designer drug, a form of chemogenetics.
Engineered G protein-coupled receptors activated by otherwise inert drug-like small molecules provide a chemogenetic or pharmacogenetic approach for selective remote control of neuronal activity.
Recent advance in genetics has also allowed development of novel pharmacological tools to selectively and remotely control neuronal activity using engineered G protein-coupled receptors, which can be activated by otherwise inert drug-like small molecules such as the designer receptors exclusively activated by designer drug, a form of chemogenetics.
Engineered G protein-coupled receptors activated by otherwise inert drug-like small molecules provide a chemogenetic or pharmacogenetic approach for selective remote control of neuronal activity.
Recent advance in genetics has also allowed development of novel pharmacological tools to selectively and remotely control neuronal activity using engineered G protein-coupled receptors, which can be activated by otherwise inert drug-like small molecules such as the designer receptors exclusively activated by designer drug, a form of chemogenetics.
Engineered G protein-coupled receptors activated by otherwise inert drug-like small molecules provide a chemogenetic or pharmacogenetic approach for selective remote control of neuronal activity.
Recent advance in genetics has also allowed development of novel pharmacological tools to selectively and remotely control neuronal activity using engineered G protein-coupled receptors, which can be activated by otherwise inert drug-like small molecules such as the designer receptors exclusively activated by designer drug, a form of chemogenetics.
Optogenetics and pharmacogenetics allow selective and bidirectional modulation of defined neuronal populations with unprecedented specificity.
The cutting-edge optogenetics and pharmacogenetics are powerful tools in neuroscience that allow selective and bidirectional modulation of the activity of defined populations of neurons with unprecedented specificity.
Optogenetics and pharmacogenetics allow selective and bidirectional modulation of defined neuronal populations with unprecedented specificity.
The cutting-edge optogenetics and pharmacogenetics are powerful tools in neuroscience that allow selective and bidirectional modulation of the activity of defined populations of neurons with unprecedented specificity.
Optogenetics and pharmacogenetics allow selective and bidirectional modulation of defined neuronal populations with unprecedented specificity.
The cutting-edge optogenetics and pharmacogenetics are powerful tools in neuroscience that allow selective and bidirectional modulation of the activity of defined populations of neurons with unprecedented specificity.
Optogenetics and pharmacogenetics allow selective and bidirectional modulation of defined neuronal populations with unprecedented specificity.
The cutting-edge optogenetics and pharmacogenetics are powerful tools in neuroscience that allow selective and bidirectional modulation of the activity of defined populations of neurons with unprecedented specificity.
Optogenetics and pharmacogenetics allow selective and bidirectional modulation of defined neuronal populations with unprecedented specificity.
The cutting-edge optogenetics and pharmacogenetics are powerful tools in neuroscience that allow selective and bidirectional modulation of the activity of defined populations of neurons with unprecedented specificity.
Optogenetics and pharmacogenetics allow selective and bidirectional modulation of defined neuronal populations with unprecedented specificity.
The cutting-edge optogenetics and pharmacogenetics are powerful tools in neuroscience that allow selective and bidirectional modulation of the activity of defined populations of neurons with unprecedented specificity.
Optogenetics and pharmacogenetics allow selective and bidirectional modulation of defined neuronal populations with unprecedented specificity.
The cutting-edge optogenetics and pharmacogenetics are powerful tools in neuroscience that allow selective and bidirectional modulation of the activity of defined populations of neurons with unprecedented specificity.
Approval Evidence
1 source3 linked approval claimsfirst-pass slug engineered-g-protein-coupled-receptors
development of novel pharmacological tools to selectively and remotely control neuronal activity using engineered G protein-coupled receptors
Source:
application scopesupports
These optogenetic and chemogenetic toolboxes have enabled advances in deciphering nervous system function and its influence on physiological processes in health and disease.
These novel toolboxes are enabling significant advances in deciphering how the nervous system works and its influence on various physiological processes in health and disease.
Source:
capability summarysupports
Engineered G protein-coupled receptors activated by otherwise inert drug-like small molecules provide a chemogenetic or pharmacogenetic approach for selective remote control of neuronal activity.
Recent advance in genetics has also allowed development of novel pharmacological tools to selectively and remotely control neuronal activity using engineered G protein-coupled receptors, which can be activated by otherwise inert drug-like small molecules such as the designer receptors exclusively activated by designer drug, a form of chemogenetics.
Source:
field level assessmentsupports
Optogenetics and pharmacogenetics allow selective and bidirectional modulation of defined neuronal populations with unprecedented specificity.
The cutting-edge optogenetics and pharmacogenetics are powerful tools in neuroscience that allow selective and bidirectional modulation of the activity of defined populations of neurons with unprecedented specificity.
Source:
Comparisons
Source-backed strengths
The main supported advantage is selective remote control of neuronal activity through engineered GPCRs responsive to otherwise inert drug-like small molecules. The evidence also situates pharmacogenetic tools as useful for studying nervous system function in health and disease, but it does not provide quantitative performance data for this specific receptor class.
Ranked Citations
- 1.