Source 1primary paper2021
Toolkit/engineered MT-cleaving enzymes
engineered MT-cleaving enzymes
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Engineered MT-cleaving enzymes are recruitment-based perturbation tools that acutely disassemble selected microtubule subtypes in living cells. Reported implementations use chemogenetic or optogenetic recruitment to localize microtubule-cleaving activity and thereby control microtubule disassembly.
Usefulness & Problems
Why this is useful
These tools enable perturbation of specific microtubule populations with faster and more localized effects than conventional microtubule-targeting agents. In the cited study, acute disassembly was used to rapidly interrogate microtubule-dependent vesicular trafficking and lysosome dynamics.
Source:
we have used chemogenetics and optogenetics to disassemble specific MT subtypes by rapid recruitment of engineered MT-cleaving enzymes
Source:
Acute MT disassembly swiftly halted vesicular trafficking and lysosome dynamics.
Problem solved
Conventional microtubule-targeting agents act slowly and affect the entire cellular microtubule pool, limiting analysis of dynamic mechanisms in specific microtubule populations. Engineered MT-cleaving enzymes address this by enabling acute, targeted disassembly of selected microtubule subtypes.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete method used to build, optimize, or evolve an engineered system.
Techniques
No technique tags yet.
Target processes
localizationInput: Light
Implementation Constraints
Reported implementations rely on chemogenetic or optogenetic recruitment to localize engineered MT-cleaving activity in living cells. The available evidence does not specify construct architecture, cofactors, expression system, delivery method, or illumination parameters.
The supplied evidence does not identify the enzyme scaffold, recruitment modules, optical wavelengths, or quantitative performance metrics such as kinetics, reversibility, or subtype specificity. Validation is described from a single 2021 source, so independent replication and breadth across cell types or organisms are not established here.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Conventional microtubule-targeting agents are insufficient to dissect dynamic mechanisms of specific microtubule populations because their effects are slow and act on the entire microtubule pool in cells.
MT-targeting agents, which, however, are insufficient to dissect the dynamic mechanisms of specific MT populations due to their slow effects on the entire pool of MTs in cells
Conventional microtubule-targeting agents are insufficient to dissect dynamic mechanisms of specific microtubule populations because their effects are slow and act on the entire microtubule pool in cells.
MT-targeting agents, which, however, are insufficient to dissect the dynamic mechanisms of specific MT populations due to their slow effects on the entire pool of MTs in cells
Conventional microtubule-targeting agents are insufficient to dissect dynamic mechanisms of specific microtubule populations because their effects are slow and act on the entire microtubule pool in cells.
MT-targeting agents, which, however, are insufficient to dissect the dynamic mechanisms of specific MT populations due to their slow effects on the entire pool of MTs in cells
Conventional microtubule-targeting agents are insufficient to dissect dynamic mechanisms of specific microtubule populations because their effects are slow and act on the entire microtubule pool in cells.
MT-targeting agents, which, however, are insufficient to dissect the dynamic mechanisms of specific MT populations due to their slow effects on the entire pool of MTs in cells
Conventional microtubule-targeting agents are insufficient to dissect dynamic mechanisms of specific microtubule populations because their effects are slow and act on the entire microtubule pool in cells.
MT-targeting agents, which, however, are insufficient to dissect the dynamic mechanisms of specific MT populations due to their slow effects on the entire pool of MTs in cells
Conventional microtubule-targeting agents are insufficient to dissect dynamic mechanisms of specific microtubule populations because their effects are slow and act on the entire microtubule pool in cells.
MT-targeting agents, which, however, are insufficient to dissect the dynamic mechanisms of specific MT populations due to their slow effects on the entire pool of MTs in cells
Conventional microtubule-targeting agents are insufficient to dissect dynamic mechanisms of specific microtubule populations because their effects are slow and act on the entire microtubule pool in cells.
MT-targeting agents, which, however, are insufficient to dissect the dynamic mechanisms of specific MT populations due to their slow effects on the entire pool of MTs in cells
Chemogenetic and optogenetic recruitment of engineered MT-cleaving enzymes can disassemble specific microtubule subtypes.
we have used chemogenetics and optogenetics to disassemble specific MT subtypes by rapid recruitment of engineered MT-cleaving enzymes
Chemogenetic and optogenetic recruitment of engineered MT-cleaving enzymes can disassemble specific microtubule subtypes.
we have used chemogenetics and optogenetics to disassemble specific MT subtypes by rapid recruitment of engineered MT-cleaving enzymes
Chemogenetic and optogenetic recruitment of engineered MT-cleaving enzymes can disassemble specific microtubule subtypes.
we have used chemogenetics and optogenetics to disassemble specific MT subtypes by rapid recruitment of engineered MT-cleaving enzymes
Chemogenetic and optogenetic recruitment of engineered MT-cleaving enzymes can disassemble specific microtubule subtypes.
we have used chemogenetics and optogenetics to disassemble specific MT subtypes by rapid recruitment of engineered MT-cleaving enzymes
Chemogenetic and optogenetic recruitment of engineered MT-cleaving enzymes can disassemble specific microtubule subtypes.
we have used chemogenetics and optogenetics to disassemble specific MT subtypes by rapid recruitment of engineered MT-cleaving enzymes
Chemogenetic and optogenetic recruitment of engineered MT-cleaving enzymes can disassemble specific microtubule subtypes.
we have used chemogenetics and optogenetics to disassemble specific MT subtypes by rapid recruitment of engineered MT-cleaving enzymes
Chemogenetic and optogenetic recruitment of engineered MT-cleaving enzymes can disassemble specific microtubule subtypes.
we have used chemogenetics and optogenetics to disassemble specific MT subtypes by rapid recruitment of engineered MT-cleaving enzymes
Acute microtubule disassembly swiftly halted vesicular trafficking and lysosome dynamics.
Acute MT disassembly swiftly halted vesicular trafficking and lysosome dynamics.
Acute microtubule disassembly swiftly halted vesicular trafficking and lysosome dynamics.
Acute MT disassembly swiftly halted vesicular trafficking and lysosome dynamics.
Acute microtubule disassembly swiftly halted vesicular trafficking and lysosome dynamics.
Acute MT disassembly swiftly halted vesicular trafficking and lysosome dynamics.
Acute microtubule disassembly swiftly halted vesicular trafficking and lysosome dynamics.
Acute MT disassembly swiftly halted vesicular trafficking and lysosome dynamics.
Acute microtubule disassembly swiftly halted vesicular trafficking and lysosome dynamics.
Acute MT disassembly swiftly halted vesicular trafficking and lysosome dynamics.
Acute microtubule disassembly swiftly halted vesicular trafficking and lysosome dynamics.
Acute MT disassembly swiftly halted vesicular trafficking and lysosome dynamics.
Acute microtubule disassembly swiftly halted vesicular trafficking and lysosome dynamics.
Acute MT disassembly swiftly halted vesicular trafficking and lysosome dynamics.
The induced effects were rapidly reversible by inhibiting cleaving-enzyme activity or microtubule association.
These effects were rapidly reversed by inhibiting the activity or MT association of the cleaving enzymes.
The induced effects were rapidly reversible by inhibiting cleaving-enzyme activity or microtubule association.
These effects were rapidly reversed by inhibiting the activity or MT association of the cleaving enzymes.
The induced effects were rapidly reversible by inhibiting cleaving-enzyme activity or microtubule association.
These effects were rapidly reversed by inhibiting the activity or MT association of the cleaving enzymes.
The induced effects were rapidly reversible by inhibiting cleaving-enzyme activity or microtubule association.
These effects were rapidly reversed by inhibiting the activity or MT association of the cleaving enzymes.
The induced effects were rapidly reversible by inhibiting cleaving-enzyme activity or microtubule association.
These effects were rapidly reversed by inhibiting the activity or MT association of the cleaving enzymes.
The induced effects were rapidly reversible by inhibiting cleaving-enzyme activity or microtubule association.
These effects were rapidly reversed by inhibiting the activity or MT association of the cleaving enzymes.
The induced effects were rapidly reversible by inhibiting cleaving-enzyme activity or microtubule association.
These effects were rapidly reversed by inhibiting the activity or MT association of the cleaving enzymes.
The approach was used to disassemble tyrosinated microtubules and microtubule-based structures including primary cilia, mitotic spindles, and intercellular bridges.
We also used this approach to disassemble MTs specifically modified by tyrosination and several MT-based structures including primary cilia, mitotic spindles, and intercellular bridges.
The approach was used to disassemble tyrosinated microtubules and microtubule-based structures including primary cilia, mitotic spindles, and intercellular bridges.
We also used this approach to disassemble MTs specifically modified by tyrosination and several MT-based structures including primary cilia, mitotic spindles, and intercellular bridges.
The approach was used to disassemble tyrosinated microtubules and microtubule-based structures including primary cilia, mitotic spindles, and intercellular bridges.
We also used this approach to disassemble MTs specifically modified by tyrosination and several MT-based structures including primary cilia, mitotic spindles, and intercellular bridges.
The approach was used to disassemble tyrosinated microtubules and microtubule-based structures including primary cilia, mitotic spindles, and intercellular bridges.
We also used this approach to disassemble MTs specifically modified by tyrosination and several MT-based structures including primary cilia, mitotic spindles, and intercellular bridges.
The approach was used to disassemble tyrosinated microtubules and microtubule-based structures including primary cilia, mitotic spindles, and intercellular bridges.
We also used this approach to disassemble MTs specifically modified by tyrosination and several MT-based structures including primary cilia, mitotic spindles, and intercellular bridges.
The approach was used to disassemble tyrosinated microtubules and microtubule-based structures including primary cilia, mitotic spindles, and intercellular bridges.
We also used this approach to disassemble MTs specifically modified by tyrosination and several MT-based structures including primary cilia, mitotic spindles, and intercellular bridges.
The approach was used to disassemble tyrosinated microtubules and microtubule-based structures including primary cilia, mitotic spindles, and intercellular bridges.
We also used this approach to disassemble MTs specifically modified by tyrosination and several MT-based structures including primary cilia, mitotic spindles, and intercellular bridges.
Approval Evidence
1 source5 linked approval claimsfirst-pass slug engineered-mt-cleaving-enzymes
rapid recruitment of engineered MT-cleaving enzymes
Source:
advantage over prior methodssupports
Conventional microtubule-targeting agents are insufficient to dissect dynamic mechanisms of specific microtubule populations because their effects are slow and act on the entire microtubule pool in cells.
MT-targeting agents, which, however, are insufficient to dissect the dynamic mechanisms of specific MT populations due to their slow effects on the entire pool of MTs in cells
Source:
capabilitysupports
Chemogenetic and optogenetic recruitment of engineered MT-cleaving enzymes can disassemble specific microtubule subtypes.
we have used chemogenetics and optogenetics to disassemble specific MT subtypes by rapid recruitment of engineered MT-cleaving enzymes
Source:
functional effectsupports
Acute microtubule disassembly swiftly halted vesicular trafficking and lysosome dynamics.
Acute MT disassembly swiftly halted vesicular trafficking and lysosome dynamics.
Source:
reversibilitysupports
The induced effects were rapidly reversible by inhibiting cleaving-enzyme activity or microtubule association.
These effects were rapidly reversed by inhibiting the activity or MT association of the cleaving enzymes.
Source:
target scopesupports
The approach was used to disassemble tyrosinated microtubules and microtubule-based structures including primary cilia, mitotic spindles, and intercellular bridges.
We also used this approach to disassemble MTs specifically modified by tyrosination and several MT-based structures including primary cilia, mitotic spindles, and intercellular bridges.
Source:
Comparisons
Source-backed strengths
The reported systems support rapid recruitment of engineered MT-cleaving enzymes and disassembly of specific microtubule subtypes in living cells. Acute microtubule disassembly swiftly halted vesicular trafficking and lysosome dynamics, demonstrating strong functional impact on microtubule-dependent processes.
Source:
MT-targeting agents, which, however, are insufficient to dissect the dynamic mechanisms of specific MT populations due to their slow effects on the entire pool of MTs in cells
Ranked Citations
- 1.