Toolkit/epigenetic element screening

epigenetic element screening

Assay Method·Research·Since 2018

Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.

Summary

Epigenetic element screening is described in a 2018 review as a CRISPR/Cas9-based epigenetic technique. The supplied evidence establishes only that it belongs to the set of CRISPR/Cas9-enabled approaches used in epigenetics, without further methodological detail.

Usefulness & Problems

Why this is useful

Based on the review context, this approach is useful as part of the expanded experimental repertoire that CRISPR/Cas9 has brought to epigenetics. The available evidence does not specify the assay format, readout, or biological endpoints measured by the screening strategy.

Problem solved

The evidence supports that this method addresses an epigenetics-related screening problem within the broader CRISPR/Cas9 toolkit. However, the specific problem solved, such as identifying regulatory DNA elements or mapping chromatin function, is not stated in the supplied sources.

Problem links

Need better screening or enrichment leverage

Derived

Epigenetic element screening is described in a 2018 review as one of several novel epigenetic techniques enabled by the CRISPR/Cas9 system. The supplied evidence establishes only that it is a CRISPR/Cas9-based epigenetic screening approach, without further methodological detail.

Need conditional recombination or state switching

Derived

Epigenetic element screening is described in a 2018 review as one of several novel epigenetic techniques enabled by the CRISPR/Cas9 system. The supplied evidence establishes only that it is a CRISPR/Cas9-based epigenetic screening approach, without further methodological detail.

Need controllable genome or transcript editing

Derived

Epigenetic element screening is described in a 2018 review as one of several novel epigenetic techniques enabled by the CRISPR/Cas9 system. The supplied evidence establishes only that it is a CRISPR/Cas9-based epigenetic screening approach, without further methodological detail.

Taxonomy & Function

Primary hierarchy

Technique Branch

Method: A concrete measurement method used to characterize an engineered system.

Mechanisms

No mechanism tags yet.

Target processes

editingrecombinationselection

Implementation Constraints

cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor

The only implementation detail supported by the evidence is that the method is CRISPR/Cas9-based. No information is provided on guide RNA design, nuclease activity, effector domains, delivery system, cell type, or selection/readout strategy.

The evidence is limited to review-level mention and does not define construct design, Cas9 variant usage, screening scale, or assay outputs. There is no primary experimental validation, organismal context, or benchmark information in the supplied material.

Validation

Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication

Supporting Sources

Ranked Claims

Claim 1field impactsupports2018Source 1needs review

The emergence of CRISPR/Cas9 technology has provided new routes into the epigenetics field.

In recent years, the emergence of CRISPR/Cas9 technology has provided us with new routes to the epigenetic field.
Claim 2field impactsupports2018Source 1needs review

The emergence of CRISPR/Cas9 technology has provided new routes into the epigenetics field.

In recent years, the emergence of CRISPR/Cas9 technology has provided us with new routes to the epigenetic field.
Claim 3field impactsupports2018Source 1needs review

The emergence of CRISPR/Cas9 technology has provided new routes into the epigenetics field.

In recent years, the emergence of CRISPR/Cas9 technology has provided us with new routes to the epigenetic field.
Claim 4field impactsupports2018Source 1needs review

The emergence of CRISPR/Cas9 technology has provided new routes into the epigenetics field.

In recent years, the emergence of CRISPR/Cas9 technology has provided us with new routes to the epigenetic field.
Claim 5field impactsupports2018Source 1needs review

The emergence of CRISPR/Cas9 technology has provided new routes into the epigenetics field.

In recent years, the emergence of CRISPR/Cas9 technology has provided us with new routes to the epigenetic field.
Claim 6field impactsupports2018Source 1needs review

The emergence of CRISPR/Cas9 technology has provided new routes into the epigenetics field.

In recent years, the emergence of CRISPR/Cas9 technology has provided us with new routes to the epigenetic field.
Claim 7field impactsupports2018Source 1needs review

The emergence of CRISPR/Cas9 technology has provided new routes into the epigenetics field.

In recent years, the emergence of CRISPR/Cas9 technology has provided us with new routes to the epigenetic field.
Claim 8field impactsupports2018Source 1needs review

The emergence of CRISPR/Cas9 technology has provided new routes into the epigenetics field.

In recent years, the emergence of CRISPR/Cas9 technology has provided us with new routes to the epigenetic field.
Claim 9field impactsupports2018Source 1needs review

The emergence of CRISPR/Cas9 technology has provided new routes into the epigenetics field.

In recent years, the emergence of CRISPR/Cas9 technology has provided us with new routes to the epigenetic field.
Claim 10field impactsupports2018Source 1needs review

The emergence of CRISPR/Cas9 technology has provided new routes into the epigenetics field.

In recent years, the emergence of CRISPR/Cas9 technology has provided us with new routes to the epigenetic field.
Claim 11review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.
Claim 12review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.
Claim 13review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.
Claim 14review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.
Claim 15review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.
Claim 16review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.
Claim 17review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.
Claim 18review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.
Claim 19review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.
Claim 20review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.
Claim 21review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.
Claim 22review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.
Claim 23review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.
Claim 24review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.
Claim 25review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.
Claim 26review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.
Claim 27review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.
Claim 28review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.
Claim 29review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.
Claim 30review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.
Claim 31review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.
Claim 32review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.
Claim 33review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.
Claim 34review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.
Claim 35review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.
Claim 36review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.
Claim 37review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

Approval Evidence

1 source1 linked approval claimfirst-pass slug epigenetic-element-screening
In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including ... epigenetic element screening.

Source:

review scopesupports

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

Source:

Comparisons

Source-backed strengths

A stated strength is its inclusion among novel epigenetic techniques enabled by CRISPR/Cas9, indicating conceptual relevance to modern epigenetics workflows. No direct performance data, validation results, or comparative advantages are provided in the supplied evidence.

epigenetic element screening and chromatin in vivo imaging address a similar problem space because they share editing, recombination, selection.

Shared frame: same top-level item type; shared target processes: editing, recombination, selection

epigenetic element screening and high throughput screening address a similar problem space because they share editing, recombination, selection.

Shared frame: same top-level item type; shared target processes: editing, recombination, selection

epigenetic element screening and whole genome screening of gene knockout mutants address a similar problem space because they share editing, recombination, selection.

Shared frame: same top-level item type; shared target processes: editing, recombination, selection

Ranked Citations

  1. 1.
    StructuralSource 1Stem Cells International2018Claim 10Claim 10Claim 10

    Seeded from load plan for claim cl1. Extracted from this source document.