Source 1primary paper2024
Toolkit/experimental pipeline for creating designer Nodal signaling patterns
experimental pipeline for creating designer Nodal signaling patterns
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
This tool is an experimental pipeline, together with new optogenetic reagents, for creating designer Nodal signaling patterns in live zebrafish embryos. It uses patterned light to impose synthetic Nodal activation states in vivo and control developmental signaling outputs.
Usefulness & Problems
Why this is useful
The pipeline is useful for experimentally specifying spatiotemporal Nodal signaling patterns in a living vertebrate embryo. Source evidence indicates that these imposed patterns can direct endodermal precursor internalization and can generate synthetic signaling states in Nodal pathway mutants.
Source:
The improved optoNodal2 reagents eliminate dark activity and improve response kinetics, without sacrificing dynamic range.
Problem solved
It addresses the problem of how to create defined, designer Nodal signaling patterns in vivo rather than relying only on endogenous embryonic pattern formation. The reported application is control of developmental patterning outputs in live zebrafish embryos, including rescue of defects in Nodal signaling mutants.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete method used to build, optimize, or evolve an engineered system.
Techniques
Computational DesignTarget processes
recombinationsignalingInput: Light
Implementation Constraints
The implementation involves new optogenetic reagents and an experimental pipeline applied in live zebrafish embryos. The available evidence supports the use of patterned illumination to create synthetic Nodal activation patterns, but it does not provide construct architecture, cofactor requirements, delivery strategy, or expression details.
The supplied evidence does not specify the molecular identity of the optogenetic reagents, the illumination wavelengths, or quantitative performance metrics such as spatial resolution, kinetics, or dynamic range. Validation is described in zebrafish embryos and Nodal-related developmental phenotypes, with no independent replication provided in the supplied evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Patterned Nodal activation drove precisely controlled internalization of endodermal precursors.
Patterned Nodal activation drove precisely controlled internalization of endodermal precursors.
Patterned Nodal activation drove precisely controlled internalization of endodermal precursors.
Patterned Nodal activation drove precisely controlled internalization of endodermal precursors.
Patterned Nodal activation drove precisely controlled internalization of endodermal precursors.
Patterned Nodal activation drove precisely controlled internalization of endodermal precursors.
Patterned Nodal activation drove precisely controlled internalization of endodermal precursors.
Patterned Nodal activation drove precisely controlled internalization of endodermal precursors.
Patterned Nodal activation drove precisely controlled internalization of endodermal precursors.
Patterned Nodal activation drove precisely controlled internalization of endodermal precursors.
Patterned Nodal activation drove precisely controlled internalization of endodermal precursors.
Patterned Nodal activation drove precisely controlled internalization of endodermal precursors.
Patterned Nodal activation drove precisely controlled internalization of endodermal precursors.
Patterned Nodal activation drove precisely controlled internalization of endodermal precursors.
Patterned illumination generated synthetic signaling patterns in Nodal signaling mutants and rescued several characteristic developmental defects.
we used patterned illumination to generate synthetic signaling patterns in Nodal signaling mutants, rescuing several characteristic developmental defects
Patterned illumination generated synthetic signaling patterns in Nodal signaling mutants and rescued several characteristic developmental defects.
we used patterned illumination to generate synthetic signaling patterns in Nodal signaling mutants, rescuing several characteristic developmental defects
Patterned illumination generated synthetic signaling patterns in Nodal signaling mutants and rescued several characteristic developmental defects.
we used patterned illumination to generate synthetic signaling patterns in Nodal signaling mutants, rescuing several characteristic developmental defects
Patterned illumination generated synthetic signaling patterns in Nodal signaling mutants and rescued several characteristic developmental defects.
we used patterned illumination to generate synthetic signaling patterns in Nodal signaling mutants, rescuing several characteristic developmental defects
Patterned illumination generated synthetic signaling patterns in Nodal signaling mutants and rescued several characteristic developmental defects.
we used patterned illumination to generate synthetic signaling patterns in Nodal signaling mutants, rescuing several characteristic developmental defects
Patterned illumination generated synthetic signaling patterns in Nodal signaling mutants and rescued several characteristic developmental defects.
we used patterned illumination to generate synthetic signaling patterns in Nodal signaling mutants, rescuing several characteristic developmental defects
Patterned illumination generated synthetic signaling patterns in Nodal signaling mutants and rescued several characteristic developmental defects.
we used patterned illumination to generate synthetic signaling patterns in Nodal signaling mutants, rescuing several characteristic developmental defects
An adapted ultra-widefield microscopy platform enabled parallel light patterning in up to 36 embryos and precise spatial control over Nodal signaling activity and downstream gene expression.
We adapted an ultra-widefield microscopy platform for parallel light patterning in up to 36 embryos and demonstrated precise spatial control over Nodal signaling activity and downstream gene expression.
parallel light patterned embryos 36 embryos
An adapted ultra-widefield microscopy platform enabled parallel light patterning in up to 36 embryos and precise spatial control over Nodal signaling activity and downstream gene expression.
We adapted an ultra-widefield microscopy platform for parallel light patterning in up to 36 embryos and demonstrated precise spatial control over Nodal signaling activity and downstream gene expression.
parallel light patterned embryos 36 embryos
An adapted ultra-widefield microscopy platform enabled parallel light patterning in up to 36 embryos and precise spatial control over Nodal signaling activity and downstream gene expression.
We adapted an ultra-widefield microscopy platform for parallel light patterning in up to 36 embryos and demonstrated precise spatial control over Nodal signaling activity and downstream gene expression.
parallel light patterned embryos 36 embryos
An adapted ultra-widefield microscopy platform enabled parallel light patterning in up to 36 embryos and precise spatial control over Nodal signaling activity and downstream gene expression.
We adapted an ultra-widefield microscopy platform for parallel light patterning in up to 36 embryos and demonstrated precise spatial control over Nodal signaling activity and downstream gene expression.
parallel light patterned embryos 36 embryos
An adapted ultra-widefield microscopy platform enabled parallel light patterning in up to 36 embryos and precise spatial control over Nodal signaling activity and downstream gene expression.
We adapted an ultra-widefield microscopy platform for parallel light patterning in up to 36 embryos and demonstrated precise spatial control over Nodal signaling activity and downstream gene expression.
parallel light patterned embryos 36 embryos
An adapted ultra-widefield microscopy platform enabled parallel light patterning in up to 36 embryos and precise spatial control over Nodal signaling activity and downstream gene expression.
We adapted an ultra-widefield microscopy platform for parallel light patterning in up to 36 embryos and demonstrated precise spatial control over Nodal signaling activity and downstream gene expression.
parallel light patterned embryos 36 embryos
An adapted ultra-widefield microscopy platform enabled parallel light patterning in up to 36 embryos and precise spatial control over Nodal signaling activity and downstream gene expression.
We adapted an ultra-widefield microscopy platform for parallel light patterning in up to 36 embryos and demonstrated precise spatial control over Nodal signaling activity and downstream gene expression.
parallel light patterned embryos 36 embryos
Improved optoNodal2 reagents eliminate dark activity and improve response kinetics without sacrificing dynamic range.
The improved optoNodal2 reagents eliminate dark activity and improve response kinetics, without sacrificing dynamic range.
Improved optoNodal2 reagents eliminate dark activity and improve response kinetics without sacrificing dynamic range.
The improved optoNodal2 reagents eliminate dark activity and improve response kinetics, without sacrificing dynamic range.
Improved optoNodal2 reagents eliminate dark activity and improve response kinetics without sacrificing dynamic range.
The improved optoNodal2 reagents eliminate dark activity and improve response kinetics, without sacrificing dynamic range.
Improved optoNodal2 reagents eliminate dark activity and improve response kinetics without sacrificing dynamic range.
The improved optoNodal2 reagents eliminate dark activity and improve response kinetics, without sacrificing dynamic range.
Improved optoNodal2 reagents eliminate dark activity and improve response kinetics without sacrificing dynamic range.
The improved optoNodal2 reagents eliminate dark activity and improve response kinetics, without sacrificing dynamic range.
Improved optoNodal2 reagents eliminate dark activity and improve response kinetics without sacrificing dynamic range.
The improved optoNodal2 reagents eliminate dark activity and improve response kinetics, without sacrificing dynamic range.
Improved optoNodal2 reagents eliminate dark activity and improve response kinetics without sacrificing dynamic range.
The improved optoNodal2 reagents eliminate dark activity and improve response kinetics, without sacrificing dynamic range.
Approval Evidence
1 source3 linked approval claimsfirst-pass slug experimental-pipeline-for-creating-designer-nodal-signaling-patterns
we present new optogenetic reagents and an experimental pipeline for creating designer Nodal signaling patterns in live zebrafish embryos
Source:
developmental controlsupports
Patterned Nodal activation drove precisely controlled internalization of endodermal precursors.
Patterned Nodal activation drove precisely controlled internalization of endodermal precursors.
Source:
rescuesupports
Patterned illumination generated synthetic signaling patterns in Nodal signaling mutants and rescued several characteristic developmental defects.
we used patterned illumination to generate synthetic signaling patterns in Nodal signaling mutants, rescuing several characteristic developmental defects
Source:
spatiotemporal controlsupports
An adapted ultra-widefield microscopy platform enabled parallel light patterning in up to 36 embryos and precise spatial control over Nodal signaling activity and downstream gene expression.
We adapted an ultra-widefield microscopy platform for parallel light patterning in up to 36 embryos and demonstrated precise spatial control over Nodal signaling activity and downstream gene expression.
Source:
Comparisons
Source-backed strengths
Reported strengths are functional control over developmental outcomes in vivo: patterned Nodal activation drove precisely controlled internalization of endodermal precursors. Patterned illumination also generated synthetic signaling patterns in Nodal signaling mutants and rescued several characteristic developmental defects.
Ranked Citations
- 1.