Source 1primary paper2020Nature Communications
Toolkit/far-red light-induced split Cre-loxP system
far-red light-induced split Cre-loxP system
Also known as: FISC system
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The far-red light-induced split Cre-loxP system (FISC system) is a multi-component optogenetic genome-engineering tool built from a bacteriophytochrome-based light-responsive system and split Cre recombinase. It enables far-red-light-controlled Cre-loxP recombination for non-invasive, spatiotemporally regulated genome engineering in living systems and mice.
Usefulness & Problems
Why this is useful
FISC is useful for controlling DNA recombination with far-red light, providing temporal and spatial regulation without invasive intervention. The reported in vivo organ penetration and superior liver recombination induction relative to two blue-light-based Cre systems support its value for deep-tissue applications.
Source:
Thus, the FISC system expands the optogenetic toolbox for DNA recombination to achieve spatiotemporally controlled, non-invasive genome engineering in living systems.
Problem solved
FISC addresses the problem of achieving non-invasive, spatiotemporally controlled Cre-loxP genome engineering in vivo. It specifically extends optogenetic DNA recombination beyond more weakly penetrating blue-light approaches by using a far-red-responsive bacteriophytochrome system.
Source:
Thus, the FISC system expands the optogenetic toolbox for DNA recombination to achieve spatiotemporally controlled, non-invasive genome engineering in living systems.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
bacteriophytochrome-based photoswitchingcre-loxp site-specific recombinationlight-induced reconstitution of split cre recombinaseTechniques
No technique tags yet.
Target processes
recombinationInput: Light
Implementation Constraints
FISC is based on a bacteriophytochrome optogenetic system fused to split Cre recombinase components, indicating a multi-component construct architecture assembled by domain fusion and protein splitting. The evidence also states that the system was successfully delivered using adeno-associated virus, but the supplied material does not specify construct stoichiometry, chromophore requirements, serotype, or promoter design.
The supplied evidence does not provide quantitative recombination efficiencies, background activity, illumination parameters, or kinetics. Validation is described for living systems and mice, with a specific comparative result in liver, but broader tissue generality and independent replication are not documented here.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The FISC system expands the optogenetic toolbox for DNA recombination to enable spatiotemporally controlled, non-invasive genome engineering in living systems.
Thus, the FISC system expands the optogenetic toolbox for DNA recombination to achieve spatiotemporally controlled, non-invasive genome engineering in living systems.
The FISC system expands the optogenetic toolbox for DNA recombination to enable spatiotemporally controlled, non-invasive genome engineering in living systems.
Thus, the FISC system expands the optogenetic toolbox for DNA recombination to achieve spatiotemporally controlled, non-invasive genome engineering in living systems.
The FISC system expands the optogenetic toolbox for DNA recombination to enable spatiotemporally controlled, non-invasive genome engineering in living systems.
Thus, the FISC system expands the optogenetic toolbox for DNA recombination to achieve spatiotemporally controlled, non-invasive genome engineering in living systems.
The FISC system expands the optogenetic toolbox for DNA recombination to enable spatiotemporally controlled, non-invasive genome engineering in living systems.
Thus, the FISC system expands the optogenetic toolbox for DNA recombination to achieve spatiotemporally controlled, non-invasive genome engineering in living systems.
The FISC system expands the optogenetic toolbox for DNA recombination to enable spatiotemporally controlled, non-invasive genome engineering in living systems.
Thus, the FISC system expands the optogenetic toolbox for DNA recombination to achieve spatiotemporally controlled, non-invasive genome engineering in living systems.
The FISC system expands the optogenetic toolbox for DNA recombination to enable spatiotemporally controlled, non-invasive genome engineering in living systems.
Thus, the FISC system expands the optogenetic toolbox for DNA recombination to achieve spatiotemporally controlled, non-invasive genome engineering in living systems.
The FISC system expands the optogenetic toolbox for DNA recombination to enable spatiotemporally controlled, non-invasive genome engineering in living systems.
Thus, the FISC system expands the optogenetic toolbox for DNA recombination to achieve spatiotemporally controlled, non-invasive genome engineering in living systems.
In vivo, the FISC system shows strong organ penetration and markedly outperforms two blue-light-based Cre systems for recombination induction in the liver.
Our in vivo studies showcase the strong organ-penetration capacity of FISC system, markedly outperforming two blue-light-based Cre systems for recombination induction in the liver.
In vivo, the FISC system shows strong organ penetration and markedly outperforms two blue-light-based Cre systems for recombination induction in the liver.
Our in vivo studies showcase the strong organ-penetration capacity of FISC system, markedly outperforming two blue-light-based Cre systems for recombination induction in the liver.
In vivo, the FISC system shows strong organ penetration and markedly outperforms two blue-light-based Cre systems for recombination induction in the liver.
Our in vivo studies showcase the strong organ-penetration capacity of FISC system, markedly outperforming two blue-light-based Cre systems for recombination induction in the liver.
In vivo, the FISC system shows strong organ penetration and markedly outperforms two blue-light-based Cre systems for recombination induction in the liver.
Our in vivo studies showcase the strong organ-penetration capacity of FISC system, markedly outperforming two blue-light-based Cre systems for recombination induction in the liver.
In vivo, the FISC system shows strong organ penetration and markedly outperforms two blue-light-based Cre systems for recombination induction in the liver.
Our in vivo studies showcase the strong organ-penetration capacity of FISC system, markedly outperforming two blue-light-based Cre systems for recombination induction in the liver.
In vivo, the FISC system shows strong organ penetration and markedly outperforms two blue-light-based Cre systems for recombination induction in the liver.
Our in vivo studies showcase the strong organ-penetration capacity of FISC system, markedly outperforming two blue-light-based Cre systems for recombination induction in the liver.
In vivo, the FISC system shows strong organ penetration and markedly outperforms two blue-light-based Cre systems for recombination induction in the liver.
Our in vivo studies showcase the strong organ-penetration capacity of FISC system, markedly outperforming two blue-light-based Cre systems for recombination induction in the liver.
The FISC system was successfully deployed using adeno-associated virus delivery.
Demonstrating its strong clinical relevance, we successfully deploy a FISC system using adeno-associated virus (AAV) delivery.
The FISC system was successfully deployed using adeno-associated virus delivery.
Demonstrating its strong clinical relevance, we successfully deploy a FISC system using adeno-associated virus (AAV) delivery.
The FISC system was successfully deployed using adeno-associated virus delivery.
Demonstrating its strong clinical relevance, we successfully deploy a FISC system using adeno-associated virus (AAV) delivery.
The FISC system was successfully deployed using adeno-associated virus delivery.
Demonstrating its strong clinical relevance, we successfully deploy a FISC system using adeno-associated virus (AAV) delivery.
The FISC system was successfully deployed using adeno-associated virus delivery.
Demonstrating its strong clinical relevance, we successfully deploy a FISC system using adeno-associated virus (AAV) delivery.
The FISC system was successfully deployed using adeno-associated virus delivery.
Demonstrating its strong clinical relevance, we successfully deploy a FISC system using adeno-associated virus (AAV) delivery.
The FISC system was successfully deployed using adeno-associated virus delivery.
Demonstrating its strong clinical relevance, we successfully deploy a FISC system using adeno-associated virus (AAV) delivery.
The paper reports development of a far-red light-induced split Cre-loxP system called FISC for optogenetic regulation of genome engineering in vivo using far-red light.
Here, we develop a far-red light-induced split Cre-loxP system (FISC system) based on a bacteriophytochrome optogenetic system and split-Cre recombinase, enabling optogenetical regulation of genome engineering in vivo solely by utilizing a far-red light (FRL).
The paper reports development of a far-red light-induced split Cre-loxP system called FISC for optogenetic regulation of genome engineering in vivo using far-red light.
Here, we develop a far-red light-induced split Cre-loxP system (FISC system) based on a bacteriophytochrome optogenetic system and split-Cre recombinase, enabling optogenetical regulation of genome engineering in vivo solely by utilizing a far-red light (FRL).
The paper reports development of a far-red light-induced split Cre-loxP system called FISC for optogenetic regulation of genome engineering in vivo using far-red light.
Here, we develop a far-red light-induced split Cre-loxP system (FISC system) based on a bacteriophytochrome optogenetic system and split-Cre recombinase, enabling optogenetical regulation of genome engineering in vivo solely by utilizing a far-red light (FRL).
The paper reports development of a far-red light-induced split Cre-loxP system called FISC for optogenetic regulation of genome engineering in vivo using far-red light.
Here, we develop a far-red light-induced split Cre-loxP system (FISC system) based on a bacteriophytochrome optogenetic system and split-Cre recombinase, enabling optogenetical regulation of genome engineering in vivo solely by utilizing a far-red light (FRL).
The paper reports development of a far-red light-induced split Cre-loxP system called FISC for optogenetic regulation of genome engineering in vivo using far-red light.
Here, we develop a far-red light-induced split Cre-loxP system (FISC system) based on a bacteriophytochrome optogenetic system and split-Cre recombinase, enabling optogenetical regulation of genome engineering in vivo solely by utilizing a far-red light (FRL).
The paper reports development of a far-red light-induced split Cre-loxP system called FISC for optogenetic regulation of genome engineering in vivo using far-red light.
Here, we develop a far-red light-induced split Cre-loxP system (FISC system) based on a bacteriophytochrome optogenetic system and split-Cre recombinase, enabling optogenetical regulation of genome engineering in vivo solely by utilizing a far-red light (FRL).
The paper reports development of a far-red light-induced split Cre-loxP system called FISC for optogenetic regulation of genome engineering in vivo using far-red light.
Here, we develop a far-red light-induced split Cre-loxP system (FISC system) based on a bacteriophytochrome optogenetic system and split-Cre recombinase, enabling optogenetical regulation of genome engineering in vivo solely by utilizing a far-red light (FRL).
The FISC system exhibits low background, no detectable photocytotoxicity, and efficient far-red-light-induced DNA recombination.
The FISC system exhibits low background and no detectable photocytotoxicity, while offering efficient FRL-induced DNA recombination.
The FISC system exhibits low background, no detectable photocytotoxicity, and efficient far-red-light-induced DNA recombination.
The FISC system exhibits low background and no detectable photocytotoxicity, while offering efficient FRL-induced DNA recombination.
The FISC system exhibits low background, no detectable photocytotoxicity, and efficient far-red-light-induced DNA recombination.
The FISC system exhibits low background and no detectable photocytotoxicity, while offering efficient FRL-induced DNA recombination.
The FISC system exhibits low background, no detectable photocytotoxicity, and efficient far-red-light-induced DNA recombination.
The FISC system exhibits low background and no detectable photocytotoxicity, while offering efficient FRL-induced DNA recombination.
The FISC system exhibits low background, no detectable photocytotoxicity, and efficient far-red-light-induced DNA recombination.
The FISC system exhibits low background and no detectable photocytotoxicity, while offering efficient FRL-induced DNA recombination.
The FISC system exhibits low background, no detectable photocytotoxicity, and efficient far-red-light-induced DNA recombination.
The FISC system exhibits low background and no detectable photocytotoxicity, while offering efficient FRL-induced DNA recombination.
The FISC system exhibits low background, no detectable photocytotoxicity, and efficient far-red-light-induced DNA recombination.
The FISC system exhibits low background and no detectable photocytotoxicity, while offering efficient FRL-induced DNA recombination.
Approval Evidence
1 source5 linked approval claimsfirst-pass slug far-red-light-induced-split-cre-loxp-system
Here, we develop a far-red light-induced split Cre-loxP system (FISC system) based on a bacteriophytochrome optogenetic system and split-Cre recombinase
Source:
application scopesupports
The FISC system expands the optogenetic toolbox for DNA recombination to enable spatiotemporally controlled, non-invasive genome engineering in living systems.
Thus, the FISC system expands the optogenetic toolbox for DNA recombination to achieve spatiotemporally controlled, non-invasive genome engineering in living systems.
Source:
comparative performancesupports
In vivo, the FISC system shows strong organ penetration and markedly outperforms two blue-light-based Cre systems for recombination induction in the liver.
Our in vivo studies showcase the strong organ-penetration capacity of FISC system, markedly outperforming two blue-light-based Cre systems for recombination induction in the liver.
Source:
delivery applicationsupports
The FISC system was successfully deployed using adeno-associated virus delivery.
Demonstrating its strong clinical relevance, we successfully deploy a FISC system using adeno-associated virus (AAV) delivery.
Source:
developmentsupports
The paper reports development of a far-red light-induced split Cre-loxP system called FISC for optogenetic regulation of genome engineering in vivo using far-red light.
Here, we develop a far-red light-induced split Cre-loxP system (FISC system) based on a bacteriophytochrome optogenetic system and split-Cre recombinase, enabling optogenetical regulation of genome engineering in vivo solely by utilizing a far-red light (FRL).
Source:
performancesupports
The FISC system exhibits low background, no detectable photocytotoxicity, and efficient far-red-light-induced DNA recombination.
The FISC system exhibits low background and no detectable photocytotoxicity, while offering efficient FRL-induced DNA recombination.
Source:
Comparisons
Source-backed strengths
The system was reported to expand the optogenetic toolbox for DNA recombination in living systems. In vivo, it showed strong organ penetration and markedly outperformed two blue-light-based Cre systems for recombination induction in the liver, and it was also successfully deployed using adeno-associated virus delivery.
Source:
Our in vivo studies showcase the strong organ-penetration capacity of FISC system, markedly outperforming two blue-light-based Cre systems for recombination induction in the liver.
Source:
The FISC system exhibits low background and no detectable photocytotoxicity, while offering efficient FRL-induced DNA recombination.
Ranked Citations
- 1.