Source 1primary paper2025MED
Toolkit/Flash-Away
Flash-Away
Also known as: FLASH-AWAY
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Flash-Away is an intrabody-directed optogenetic protein degradation system that enables blue-light-triggered, targeted degradation of selected proteins. It has been reported to act on actin, MLKL, and ALFA-tagged proteins.
Usefulness & Problems
Why this is useful
Flash-Away is useful for inducible removal of specific proteins with light, allowing temporal control over protein abundance. The available evidence indicates that it supports selective degradation through intrabody-based target recognition under blue-light exposure.
Source:
This versatility is demonstrated through successful application to diverse protein targets, including actin, MLKL, and ALFA-tag fused proteins.
Source:
Upon exposure to blue light, Flash-Away enables rapid and targeted degradation of selected proteins.
Source:
we introduce an intrabody-based optogenetic toolbox named Flash-Away
Problem solved
Flash-Away addresses the problem of achieving rapid, targeted degradation of chosen proteins in a light-dependent manner. The reported application to actin, MLKL, and ALFA-tagged proteins indicates that it can be adapted to multiple targets recognized by appropriate intrabodies.
Source:
This versatility is demonstrated through successful application to diverse protein targets, including actin, MLKL, and ALFA-tag fused proteins.
Problem links
Need conditional protein clearance
DerivedFlash-Away is an intrabody-directed optogenetic protein degradation system. It couples blue-light-responsive ubiquitination mediated by the TRIM21 RING domain to target recognition by specific intrabodies, enabling rapid degradation of selected proteins.
Need precise spatiotemporal control with light input
DerivedFlash-Away is an intrabody-directed optogenetic protein degradation system. It couples blue-light-responsive ubiquitination mediated by the TRIM21 RING domain to target recognition by specific intrabodies, enabling rapid degradation of selected proteins.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
Degradationintrabody-mediated target recognitionintrabody-mediated target recognitionlight-responsive ubiquitinationlight-responsive ubiquitinationtargeted protein degradationtargeted protein degradationTechniques
No technique tags yet.
Target processes
degradationInput: Light
Implementation Constraints
application scope includes tagged targets: Truecofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: multi component delivery burdenimplementation constraint: spectral hardware requirementlight inducible: Trueoperating role: actuatoroperating role: regulatorswitch architecture: multi componentuses intrabodies: Trueuses trim21 ring domain: True
The system is described as intrabody-directed and blue-light responsive, so implementation requires a target-recognizing intrabody and light delivery in the blue range. The provided evidence does not specify the exact photoreceptor components, degradation domain composition, expression system, or cofactor requirements.
The supplied evidence is limited to a single 2025 source and two high-level claims. Quantitative performance, reversibility, degradation kinetics, off-target effects, construct architecture, and validation across organisms or cell types are not provided here.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Flash-Away was successfully applied to actin, MLKL, and ALFA-tag fused proteins.
This versatility is demonstrated through successful application to diverse protein targets, including actin, MLKL, and ALFA-tag fused proteins.
Flash-Away was successfully applied to actin, MLKL, and ALFA-tag fused proteins.
This versatility is demonstrated through successful application to diverse protein targets, including actin, MLKL, and ALFA-tag fused proteins.
Flash-Away was successfully applied to actin, MLKL, and ALFA-tag fused proteins.
This versatility is demonstrated through successful application to diverse protein targets, including actin, MLKL, and ALFA-tag fused proteins.
Flash-Away was successfully applied to actin, MLKL, and ALFA-tag fused proteins.
This versatility is demonstrated through successful application to diverse protein targets, including actin, MLKL, and ALFA-tag fused proteins.
Flash-Away was successfully applied to actin, MLKL, and ALFA-tag fused proteins.
This versatility is demonstrated through successful application to diverse protein targets, including actin, MLKL, and ALFA-tag fused proteins.
Flash-Away was successfully applied to actin, MLKL, and ALFA-tag fused proteins.
This versatility is demonstrated through successful application to diverse protein targets, including actin, MLKL, and ALFA-tag fused proteins.
Flash-Away was successfully applied to actin, MLKL, and ALFA-tag fused proteins.
This versatility is demonstrated through successful application to diverse protein targets, including actin, MLKL, and ALFA-tag fused proteins.
Flash-Away was successfully applied to actin, MLKL, and ALFA-tag fused proteins.
This versatility is demonstrated through successful application to diverse protein targets, including actin, MLKL, and ALFA-tag fused proteins.
Flash-Away was successfully applied to actin, MLKL, and ALFA-tag fused proteins.
This versatility is demonstrated through successful application to diverse protein targets, including actin, MLKL, and ALFA-tag fused proteins.
Flash-Away was successfully applied to actin, MLKL, and ALFA-tag fused proteins.
This versatility is demonstrated through successful application to diverse protein targets, including actin, MLKL, and ALFA-tag fused proteins.
Flash-Away was successfully applied to actin, MLKL, and ALFA-tag fused proteins.
This versatility is demonstrated through successful application to diverse protein targets, including actin, MLKL, and ALFA-tag fused proteins.
Flash-Away was successfully applied to actin, MLKL, and ALFA-tag fused proteins.
This versatility is demonstrated through successful application to diverse protein targets, including actin, MLKL, and ALFA-tag fused proteins.
Flash-Away was successfully applied to actin, MLKL, and ALFA-tag fused proteins.
This versatility is demonstrated through successful application to diverse protein targets, including actin, MLKL, and ALFA-tag fused proteins.
Flash-Away was successfully applied to actin, MLKL, and ALFA-tag fused proteins.
This versatility is demonstrated through successful application to diverse protein targets, including actin, MLKL, and ALFA-tag fused proteins.
Flash-Away was successfully applied to actin, MLKL, and ALFA-tag fused proteins.
This versatility is demonstrated through successful application to diverse protein targets, including actin, MLKL, and ALFA-tag fused proteins.
Flash-Away was successfully applied to actin, MLKL, and ALFA-tag fused proteins.
This versatility is demonstrated through successful application to diverse protein targets, including actin, MLKL, and ALFA-tag fused proteins.
Flash-Away was successfully applied to actin, MLKL, and ALFA-tag fused proteins.
This versatility is demonstrated through successful application to diverse protein targets, including actin, MLKL, and ALFA-tag fused proteins.
Flash-Away was successfully applied to actin, MLKL, and ALFA-tag fused proteins.
This versatility is demonstrated through successful application to diverse protein targets, including actin, MLKL, and ALFA-tag fused proteins.
Flash-Away was successfully applied to actin, MLKL, and ALFA-tag fused proteins.
This versatility is demonstrated through successful application to diverse protein targets, including actin, MLKL, and ALFA-tag fused proteins.
Flash-Away was successfully applied to actin, MLKL, and ALFA-tag fused proteins.
This versatility is demonstrated through successful application to diverse protein targets, including actin, MLKL, and ALFA-tag fused proteins.
Flash-Away was successfully applied to actin, MLKL, and ALFA-tag fused proteins.
This versatility is demonstrated through successful application to diverse protein targets, including actin, MLKL, and ALFA-tag fused proteins.
Flash-Away was successfully applied to actin, MLKL, and ALFA-tag fused proteins.
This versatility is demonstrated through successful application to diverse protein targets, including actin, MLKL, and ALFA-tag fused proteins.
Flash-Away was successfully applied to actin, MLKL, and ALFA-tag fused proteins.
This versatility is demonstrated through successful application to diverse protein targets, including actin, MLKL, and ALFA-tag fused proteins.
Blue-light exposure enables rapid and targeted degradation of selected proteins by Flash-Away.
Upon exposure to blue light, Flash-Away enables rapid and targeted degradation of selected proteins.
degradation speed rapid
Blue-light exposure enables rapid and targeted degradation of selected proteins by Flash-Away.
Upon exposure to blue light, Flash-Away enables rapid and targeted degradation of selected proteins.
degradation speed rapid
Blue-light exposure enables rapid and targeted degradation of selected proteins by Flash-Away.
Upon exposure to blue light, Flash-Away enables rapid and targeted degradation of selected proteins.
degradation speed rapid
Blue-light exposure enables rapid and targeted degradation of selected proteins by Flash-Away.
Upon exposure to blue light, Flash-Away enables rapid and targeted degradation of selected proteins.
degradation speed rapid
Blue-light exposure enables rapid and targeted degradation of selected proteins by Flash-Away.
Upon exposure to blue light, Flash-Away enables rapid and targeted degradation of selected proteins.
degradation speed rapid
Blue-light exposure enables rapid and targeted degradation of selected proteins by Flash-Away.
Upon exposure to blue light, Flash-Away enables rapid and targeted degradation of selected proteins.
degradation speed rapid
Blue-light exposure enables rapid and targeted degradation of selected proteins by Flash-Away.
Upon exposure to blue light, Flash-Away enables rapid and targeted degradation of selected proteins.
degradation speed rapid
Blue-light exposure enables rapid and targeted degradation of selected proteins by Flash-Away.
Upon exposure to blue light, Flash-Away enables rapid and targeted degradation of selected proteins.
degradation speed rapid
Blue-light exposure enables rapid and targeted degradation of selected proteins by Flash-Away.
Upon exposure to blue light, Flash-Away enables rapid and targeted degradation of selected proteins.
degradation speed rapid
Blue-light exposure enables rapid and targeted degradation of selected proteins by Flash-Away.
Upon exposure to blue light, Flash-Away enables rapid and targeted degradation of selected proteins.
degradation speed rapid
Blue-light exposure enables rapid and targeted degradation of selected proteins by Flash-Away.
Upon exposure to blue light, Flash-Away enables rapid and targeted degradation of selected proteins.
degradation speed rapid
Blue-light exposure enables rapid and targeted degradation of selected proteins by Flash-Away.
Upon exposure to blue light, Flash-Away enables rapid and targeted degradation of selected proteins.
degradation speed rapid
Blue-light exposure enables rapid and targeted degradation of selected proteins by Flash-Away.
Upon exposure to blue light, Flash-Away enables rapid and targeted degradation of selected proteins.
degradation speed rapid
Blue-light exposure enables rapid and targeted degradation of selected proteins by Flash-Away.
Upon exposure to blue light, Flash-Away enables rapid and targeted degradation of selected proteins.
degradation speed rapid
Blue-light exposure enables rapid and targeted degradation of selected proteins by Flash-Away.
Upon exposure to blue light, Flash-Away enables rapid and targeted degradation of selected proteins.
degradation speed rapid
Blue-light exposure enables rapid and targeted degradation of selected proteins by Flash-Away.
Upon exposure to blue light, Flash-Away enables rapid and targeted degradation of selected proteins.
degradation speed rapid
Blue-light exposure enables rapid and targeted degradation of selected proteins by Flash-Away.
Upon exposure to blue light, Flash-Away enables rapid and targeted degradation of selected proteins.
degradation speed rapid
Blue-light exposure enables rapid and targeted degradation of selected proteins by Flash-Away.
Upon exposure to blue light, Flash-Away enables rapid and targeted degradation of selected proteins.
degradation speed rapid
Blue-light exposure enables rapid and targeted degradation of selected proteins by Flash-Away.
Upon exposure to blue light, Flash-Away enables rapid and targeted degradation of selected proteins.
degradation speed rapid
Blue-light exposure enables rapid and targeted degradation of selected proteins by Flash-Away.
Upon exposure to blue light, Flash-Away enables rapid and targeted degradation of selected proteins.
degradation speed rapid
Blue-light exposure enables rapid and targeted degradation of selected proteins by Flash-Away.
Upon exposure to blue light, Flash-Away enables rapid and targeted degradation of selected proteins.
degradation speed rapid
Blue-light exposure enables rapid and targeted degradation of selected proteins by Flash-Away.
Upon exposure to blue light, Flash-Away enables rapid and targeted degradation of selected proteins.
degradation speed rapid
Blue-light exposure enables rapid and targeted degradation of selected proteins by Flash-Away.
Upon exposure to blue light, Flash-Away enables rapid and targeted degradation of selected proteins.
degradation speed rapid
Flash-Away couples light-responsive ubiquitination activity of the TRIM21 RING domain with specific intrabodies for precise target recognition.
Flash-Away integrates the light-responsive ubiquitination activity of the RING domain of TRIM21 for protein degradation, coupled with specific intrabodies for precise targeting.
Flash-Away couples light-responsive ubiquitination activity of the TRIM21 RING domain with specific intrabodies for precise target recognition.
Flash-Away integrates the light-responsive ubiquitination activity of the RING domain of TRIM21 for protein degradation, coupled with specific intrabodies for precise targeting.
Flash-Away couples light-responsive ubiquitination activity of the TRIM21 RING domain with specific intrabodies for precise target recognition.
Flash-Away integrates the light-responsive ubiquitination activity of the RING domain of TRIM21 for protein degradation, coupled with specific intrabodies for precise targeting.
Flash-Away couples light-responsive ubiquitination activity of the TRIM21 RING domain with specific intrabodies for precise target recognition.
Flash-Away integrates the light-responsive ubiquitination activity of the RING domain of TRIM21 for protein degradation, coupled with specific intrabodies for precise targeting.
Flash-Away couples light-responsive ubiquitination activity of the TRIM21 RING domain with specific intrabodies for precise target recognition.
Flash-Away integrates the light-responsive ubiquitination activity of the RING domain of TRIM21 for protein degradation, coupled with specific intrabodies for precise targeting.
Flash-Away couples light-responsive ubiquitination activity of the TRIM21 RING domain with specific intrabodies for precise target recognition.
Flash-Away integrates the light-responsive ubiquitination activity of the RING domain of TRIM21 for protein degradation, coupled with specific intrabodies for precise targeting.
Flash-Away couples light-responsive ubiquitination activity of the TRIM21 RING domain with specific intrabodies for precise target recognition.
Flash-Away integrates the light-responsive ubiquitination activity of the RING domain of TRIM21 for protein degradation, coupled with specific intrabodies for precise targeting.
Flash-Away couples light-responsive ubiquitination activity of the TRIM21 RING domain with specific intrabodies for precise target recognition.
Flash-Away integrates the light-responsive ubiquitination activity of the RING domain of TRIM21 for protein degradation, coupled with specific intrabodies for precise targeting.
Flash-Away couples light-responsive ubiquitination activity of the TRIM21 RING domain with specific intrabodies for precise target recognition.
Flash-Away integrates the light-responsive ubiquitination activity of the RING domain of TRIM21 for protein degradation, coupled with specific intrabodies for precise targeting.
Flash-Away couples light-responsive ubiquitination activity of the TRIM21 RING domain with specific intrabodies for precise target recognition.
Flash-Away integrates the light-responsive ubiquitination activity of the RING domain of TRIM21 for protein degradation, coupled with specific intrabodies for precise targeting.
Flash-Away couples light-responsive ubiquitination activity of the TRIM21 RING domain with specific intrabodies for precise target recognition.
Flash-Away integrates the light-responsive ubiquitination activity of the RING domain of TRIM21 for protein degradation, coupled with specific intrabodies for precise targeting.
Flash-Away couples light-responsive ubiquitination activity of the TRIM21 RING domain with specific intrabodies for precise target recognition.
Flash-Away integrates the light-responsive ubiquitination activity of the RING domain of TRIM21 for protein degradation, coupled with specific intrabodies for precise targeting.
Flash-Away couples light-responsive ubiquitination activity of the TRIM21 RING domain with specific intrabodies for precise target recognition.
Flash-Away integrates the light-responsive ubiquitination activity of the RING domain of TRIM21 for protein degradation, coupled with specific intrabodies for precise targeting.
Flash-Away couples light-responsive ubiquitination activity of the TRIM21 RING domain with specific intrabodies for precise target recognition.
Flash-Away integrates the light-responsive ubiquitination activity of the RING domain of TRIM21 for protein degradation, coupled with specific intrabodies for precise targeting.
Flash-Away couples light-responsive ubiquitination activity of the TRIM21 RING domain with specific intrabodies for precise target recognition.
Flash-Away integrates the light-responsive ubiquitination activity of the RING domain of TRIM21 for protein degradation, coupled with specific intrabodies for precise targeting.
Flash-Away couples light-responsive ubiquitination activity of the TRIM21 RING domain with specific intrabodies for precise target recognition.
Flash-Away integrates the light-responsive ubiquitination activity of the RING domain of TRIM21 for protein degradation, coupled with specific intrabodies for precise targeting.
Flash-Away couples light-responsive ubiquitination activity of the TRIM21 RING domain with specific intrabodies for precise target recognition.
Flash-Away integrates the light-responsive ubiquitination activity of the RING domain of TRIM21 for protein degradation, coupled with specific intrabodies for precise targeting.
Flash-Away couples light-responsive ubiquitination activity of the TRIM21 RING domain with specific intrabodies for precise target recognition.
Flash-Away integrates the light-responsive ubiquitination activity of the RING domain of TRIM21 for protein degradation, coupled with specific intrabodies for precise targeting.
Flash-Away couples light-responsive ubiquitination activity of the TRIM21 RING domain with specific intrabodies for precise target recognition.
Flash-Away integrates the light-responsive ubiquitination activity of the RING domain of TRIM21 for protein degradation, coupled with specific intrabodies for precise targeting.
Flash-Away couples light-responsive ubiquitination activity of the TRIM21 RING domain with specific intrabodies for precise target recognition.
Flash-Away integrates the light-responsive ubiquitination activity of the RING domain of TRIM21 for protein degradation, coupled with specific intrabodies for precise targeting.
Flash-Away couples light-responsive ubiquitination activity of the TRIM21 RING domain with specific intrabodies for precise target recognition.
Flash-Away integrates the light-responsive ubiquitination activity of the RING domain of TRIM21 for protein degradation, coupled with specific intrabodies for precise targeting.
Flash-Away couples light-responsive ubiquitination activity of the TRIM21 RING domain with specific intrabodies for precise target recognition.
Flash-Away integrates the light-responsive ubiquitination activity of the RING domain of TRIM21 for protein degradation, coupled with specific intrabodies for precise targeting.
Flash-Away couples light-responsive ubiquitination activity of the TRIM21 RING domain with specific intrabodies for precise target recognition.
Flash-Away integrates the light-responsive ubiquitination activity of the RING domain of TRIM21 for protein degradation, coupled with specific intrabodies for precise targeting.
Flash-Away is an intrabody-based optogenetic toolbox for targeted protein degradation.
we introduce an intrabody-based optogenetic toolbox named Flash-Away
Flash-Away is an intrabody-based optogenetic toolbox for targeted protein degradation.
we introduce an intrabody-based optogenetic toolbox named Flash-Away
Flash-Away is an intrabody-based optogenetic toolbox for targeted protein degradation.
we introduce an intrabody-based optogenetic toolbox named Flash-Away
Flash-Away is an intrabody-based optogenetic toolbox for targeted protein degradation.
we introduce an intrabody-based optogenetic toolbox named Flash-Away
Flash-Away is an intrabody-based optogenetic toolbox for targeted protein degradation.
we introduce an intrabody-based optogenetic toolbox named Flash-Away
Flash-Away is an intrabody-based optogenetic toolbox for targeted protein degradation.
we introduce an intrabody-based optogenetic toolbox named Flash-Away
Flash-Away is an intrabody-based optogenetic toolbox for targeted protein degradation.
we introduce an intrabody-based optogenetic toolbox named Flash-Away
Flash-Away is an intrabody-based optogenetic toolbox for targeted protein degradation.
we introduce an intrabody-based optogenetic toolbox named Flash-Away
Flash-Away is an intrabody-based optogenetic toolbox for targeted protein degradation.
we introduce an intrabody-based optogenetic toolbox named Flash-Away
Flash-Away is an intrabody-based optogenetic toolbox for targeted protein degradation.
we introduce an intrabody-based optogenetic toolbox named Flash-Away
Flash-Away is an intrabody-based optogenetic toolbox for targeted protein degradation.
we introduce an intrabody-based optogenetic toolbox named Flash-Away
Flash-Away is an intrabody-based optogenetic toolbox for targeted protein degradation.
we introduce an intrabody-based optogenetic toolbox named Flash-Away
Flash-Away is an intrabody-based optogenetic toolbox for targeted protein degradation.
we introduce an intrabody-based optogenetic toolbox named Flash-Away
Flash-Away is an intrabody-based optogenetic toolbox for targeted protein degradation.
we introduce an intrabody-based optogenetic toolbox named Flash-Away
Flash-Away is an intrabody-based optogenetic toolbox for targeted protein degradation.
we introduce an intrabody-based optogenetic toolbox named Flash-Away
Flash-Away is an intrabody-based optogenetic toolbox for targeted protein degradation.
we introduce an intrabody-based optogenetic toolbox named Flash-Away
Flash-Away is an intrabody-based optogenetic toolbox for targeted protein degradation.
we introduce an intrabody-based optogenetic toolbox named Flash-Away
Flash-Away is an intrabody-based optogenetic toolbox for targeted protein degradation.
we introduce an intrabody-based optogenetic toolbox named Flash-Away
Flash-Away is an intrabody-based optogenetic toolbox for targeted protein degradation.
we introduce an intrabody-based optogenetic toolbox named Flash-Away
Flash-Away is an intrabody-based optogenetic toolbox for targeted protein degradation.
we introduce an intrabody-based optogenetic toolbox named Flash-Away
Flash-Away is an intrabody-based optogenetic toolbox for targeted protein degradation.
we introduce an intrabody-based optogenetic toolbox named Flash-Away
Flash-Away is an intrabody-based optogenetic toolbox for targeted protein degradation.
we introduce an intrabody-based optogenetic toolbox named Flash-Away
Flash-Away is an intrabody-based optogenetic toolbox for targeted protein degradation.
we introduce an intrabody-based optogenetic toolbox named Flash-Away
Approval Evidence
1 source4 linked approval claimsfirst-pass slug flash-away
we introduce an intrabody-based optogenetic toolbox named Flash-Away. Flash-Away integrates the light-responsive ubiquitination activity of the RING domain of TRIM21 for protein degradation, coupled with specific intrabodies for precise targeting.
Source:
application scopesupports
Flash-Away was successfully applied to actin, MLKL, and ALFA-tag fused proteins.
This versatility is demonstrated through successful application to diverse protein targets, including actin, MLKL, and ALFA-tag fused proteins.
Source:
functional effectsupports
Blue-light exposure enables rapid and targeted degradation of selected proteins by Flash-Away.
Upon exposure to blue light, Flash-Away enables rapid and targeted degradation of selected proteins.
Source:
mechanismsupports
Flash-Away couples light-responsive ubiquitination activity of the TRIM21 RING domain with specific intrabodies for precise target recognition.
Flash-Away integrates the light-responsive ubiquitination activity of the RING domain of TRIM21 for protein degradation, coupled with specific intrabodies for precise targeting.
Source:
tool introductionsupports
Flash-Away is an intrabody-based optogenetic toolbox for targeted protein degradation.
we introduce an intrabody-based optogenetic toolbox named Flash-Away
Source:
Comparisons
Source-backed strengths
The main reported strength is rapid and targeted degradation upon blue-light exposure. Its demonstrated use against endogenous or specified targets including actin, MLKL, and ALFA-tag fusion proteins supports functional versatility across more than one target class.
Compared with blue light-responsive Cas13b mRNA knockdown system
Flash-Away and blue light-responsive Cas13b mRNA knockdown system address a similar problem space because they share degradation.
Shared frame: shared target processes: degradation; shared mechanisms: degradation; same primary input modality: light
Relative tradeoffs: looks easier to implement in practice.
Compared with Cry2
Flash-Away and Cry2 address a similar problem space because they share degradation.
Shared frame: same top-level item type; shared target processes: degradation; shared mechanisms: degradation; same primary input modality: light
Strengths here: may avoid an exogenous cofactor requirement.
Relative tradeoffs: appears more independently replicated.
Flash-Away and CRY2 and LOV-fused degron light-responsive repression/degradation system address a similar problem space because they share degradation.
Shared frame: same top-level item type; shared target processes: degradation; shared mechanisms: degradation; same primary input modality: light
Ranked Citations
- 1.