flexible plasmonic metasurface

Stages

1. 1.
   Metasurface fabrication(library_build)

   This stage creates the physical SERS-active flexible metasurface required for downstream single-exosome detection.

   Selection: Fabricate the plasmonic metasurface as a modulated silver nanofilm on a thin flexible plastic substrate using holographic lithography.

2. 2.
   Single-exosome discrimination and SERS acquisition(functional_characterization)

   This stage tests whether the metasurface supports the intended analytical function of distinguishing individual exosomes and collecting their spectra.

   Selection: Distinguish individual exosomes from the HEK293T cell line on the metasurface and obtain and assign their SERS spectra.

3. 3.
   Correlative multimodal investigation(secondary_characterization)

   This stage provides multimodal investigation of the metasurface and associated measurements after the primary SERS-based exosome detection step.

   Selection: Investigate the plasmonic metasurface using a correlation approach combining atomic force microscopy, scanning spreading resistance microscopy, and surface-enhanced spectroscopy.

Steps

1. 1.
   Fabricate the plasmonic metasurface by holographic lithographyengineered substrate and fabrication method

   Create the flexible plasmonic substrate used for single-exosome SERS detection.

   The metasurface must be fabricated before exosomes can be analyzed on it.

2. 2.
   Distinguish individual HEK293T exosomes on the metasurfaceSERS-active detection substrate

   Identify individual exosomes from the HEK293T cell line on the metasurface.

   Individual exosomes are first distinguished on the metasurface before their spectra are obtained and assigned.

3. 3.
   Obtain and assign SERS spectra of individual exosomesdetection substrate and correlative analytical method

   Acquire and assign SERS spectra from the individual exosomes distinguished on the metasurface.

   The abstract states that exosomes are first distinguished on the metasurface and then their SERS spectra are obtained and assigned.

4. 4.
   Investigate the metasurface with correlated AFM, scanning spreading resistance microscopy, and surface-enhanced spectroscopysubstrate under study and multimodal characterization workflow

   Further investigate the plasmonic metasurface using a correlative multimodal approach.

   The abstract presents this as a further investigation step after fabrication and the proposed exosome-detection method.