Source 1primary paper2025MED
Toolkit/FLP/FRT system
FLP/FRT system
Also known as: FLP/FRT
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
FLP/FRT and CRISPR/Cas9 homology-directed repair (HDR) strategies, as well as their combinations, are currently the most effective for SSTI in maize.
Usefulness & Problems
No literature-backed usefulness or problem-fit explainer has been materialized for this record yet.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
site-specific recombinationTechniques
Selection / EnrichmentTarget processes
editingselectionValidation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Because CRISPR/Cas9 HDR targets known insertion sites, it can effectively direct SSTI in maize while requiring fewer transgenic events.
SSTI is effectively targeted by CRISPR/Cas9 HDR, requiring a smaller number of transgenic events.
The FLP/FRT system for maize SSTI requires many transgenic events, selection of a recombinant target line, and co-transformation with a second T-DNA because insertion-site stability is not known in advance.
The FLP/FRT system still depends on generating a high number of transgenic events, selecting a recombinant target line (RTL), and co-transforming this RTL with a second T-DNA, since there is no prior information on whether the insertion site is considered stable.
CRISPR/Cas9 HDR for maize SSTI requires prior information about the insertion site.
CRISPR/Cas9 HDR requires prior information about the insertion site.
FLP/FRT and CRISPR/Cas9 HDR strategies, including combinations of them, are currently the most effective approaches for site-specific transgene insertion in maize.
FLP/FRT and CRISPR/Cas9 homology-directed repair (HDR) strategies, as well as their combinations, are currently the most effective for SSTI in maize.
Approval Evidence
1 source2 linked approval claimsfirst-pass slug flp-frt-system
FLP/FRT and CRISPR/Cas9 homology-directed repair (HDR) strategies, as well as their combinations, are currently the most effective for SSTI in maize.
Source:
limitationsupports
The FLP/FRT system for maize SSTI requires many transgenic events, selection of a recombinant target line, and co-transformation with a second T-DNA because insertion-site stability is not known in advance.
The FLP/FRT system still depends on generating a high number of transgenic events, selecting a recombinant target line (RTL), and co-transforming this RTL with a second T-DNA, since there is no prior information on whether the insertion site is considered stable.
Source:
state of the artsupports
FLP/FRT and CRISPR/Cas9 HDR strategies, including combinations of them, are currently the most effective approaches for site-specific transgene insertion in maize.
FLP/FRT and CRISPR/Cas9 homology-directed repair (HDR) strategies, as well as their combinations, are currently the most effective for SSTI in maize.
Source:
Comparisons
No literature-backed comparison notes have been materialized for this record yet.
Ranked Citations
- 1.