Source 1review2022FEBS Letters
Toolkit/fluorescent probes
fluorescent probes
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Fluorescent probes are described as an expanding assay toolbox that can be combined with larval zebrafish for real-time in vivo monitoring of cell identity, cell fate, and physiology. In the cited review, this use context is framed for organ pathophysiology studies, including the pancreas and islets of Langerhans.
Usefulness & Problems
Why this is useful
This assay approach is useful because larval zebrafish support in vivo microscopy and are presented as well matched to fluorescent probes for dynamic observation of biological states in living animals. The cited evidence specifically supports applications to monitoring cell identity, fate, and physiology during organ pathophysiology studies.
Problem solved
This tool helps address the problem of monitoring cellular states and physiological changes in real time within intact living vertebrate tissue. The supplied evidence places this need in the context of pancreas and islet pathophysiology in zebrafish larvae.
Problem links
Need tighter control over protein production
DerivedFluorescent probes are described as an expanding assay toolbox that can be combined with larval zebrafish for real-time in vivo monitoring of cell identity, cell fate, and physiology. In the cited review, this use context is framed for organ pathophysiology studies, including the pancreas and islets of Langerhans.
Published Workflows
Objective: Bridge live-cell fluorescence microscopy with electron microscopy so that specific targets can be followed dynamically and then interpreted in higher-resolution ultrastructural context.
Why it works: The review describes complementary strengths: fluorescence microscopy can follow specific targets in living cells, while electron microscopy provides higher resolution and ultrastructural context. New probe development enables these modalities to be connected in a single correlative strategy.
fluorescent labeling of specific targetselectron-microscopy visualization of organelles, membranes, and macromoleculescorrelative imaging enabled by probe compatibility across modalitiesfluorescence microscopyelectron microscopycorrelative imaging
Stages
- 1.Fluorescence microscopy of specific targets in living cells(functional_characterization)
This stage captures target-specific and dynamic information that fluorescence microscopy is well suited to provide.
Selection: Follow specific targets on or in living cells to reveal dynamic localization and/or function.
- 2.Electron microscopy for higher-resolution structural context(confirmatory_validation)
This stage addresses the resolution limitation of fluorescence microscopy and places molecules of interest relative to other cellular structures.
Selection: Use electron microscopy to achieve higher resolution and reveal organelles, membranes, and macromolecules.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Techniques
Functional AssayTarget processes
translationImplementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor
Implementation is supported in larval zebrafish in conjunction with in vivo microscopy. The available evidence does not specify construct design, delivery method, expression system, cofactors, or imaging parameters for particular probes.
The supplied evidence is review-level and does not identify specific probe chemistries, fluorescent proteins, target analytes, wavelengths, or quantitative performance metrics. It also does not provide direct evidence for translation-specific readouts despite the provided target process label.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Larval zebrafish enable in vivo microscopy for studying organ pathophysiology, including the pancreas and islets of Langerhans.
zebrafish larvae allow studying pathophysiology of many organs using in vivo microscopy. Here, we review the potential of the larval zebrafish pancreas in the context of islets of Langerhans and Type 1 diabetes.
Larval zebrafish enable in vivo microscopy for studying organ pathophysiology, including the pancreas and islets of Langerhans.
zebrafish larvae allow studying pathophysiology of many organs using in vivo microscopy. Here, we review the potential of the larval zebrafish pancreas in the context of islets of Langerhans and Type 1 diabetes.
Larval zebrafish enable in vivo microscopy for studying organ pathophysiology, including the pancreas and islets of Langerhans.
zebrafish larvae allow studying pathophysiology of many organs using in vivo microscopy. Here, we review the potential of the larval zebrafish pancreas in the context of islets of Langerhans and Type 1 diabetes.
Larval zebrafish enable in vivo microscopy for studying organ pathophysiology, including the pancreas and islets of Langerhans.
zebrafish larvae allow studying pathophysiology of many organs using in vivo microscopy. Here, we review the potential of the larval zebrafish pancreas in the context of islets of Langerhans and Type 1 diabetes.
Larval zebrafish enable in vivo microscopy for studying organ pathophysiology, including the pancreas and islets of Langerhans.
zebrafish larvae allow studying pathophysiology of many organs using in vivo microscopy. Here, we review the potential of the larval zebrafish pancreas in the context of islets of Langerhans and Type 1 diabetes.
Larval zebrafish enable in vivo microscopy for studying organ pathophysiology, including the pancreas and islets of Langerhans.
zebrafish larvae allow studying pathophysiology of many organs using in vivo microscopy. Here, we review the potential of the larval zebrafish pancreas in the context of islets of Langerhans and Type 1 diabetes.
Larval zebrafish enable in vivo microscopy for studying organ pathophysiology, including the pancreas and islets of Langerhans.
zebrafish larvae allow studying pathophysiology of many organs using in vivo microscopy. Here, we review the potential of the larval zebrafish pancreas in the context of islets of Langerhans and Type 1 diabetes.
The review states that larval zebrafish are well matched to fluorescent probes for real-time monitoring of cell identity, fate, and physiology.
We highlight the match of zebrafish larvae with the expanding toolbox of fluorescent probes that monitor cell identity, fate and/or physiology in real time.
The review states that larval zebrafish are well matched to fluorescent probes for real-time monitoring of cell identity, fate, and physiology.
We highlight the match of zebrafish larvae with the expanding toolbox of fluorescent probes that monitor cell identity, fate and/or physiology in real time.
The review states that larval zebrafish are well matched to fluorescent probes for real-time monitoring of cell identity, fate, and physiology.
We highlight the match of zebrafish larvae with the expanding toolbox of fluorescent probes that monitor cell identity, fate and/or physiology in real time.
The review states that larval zebrafish are well matched to fluorescent probes for real-time monitoring of cell identity, fate, and physiology.
We highlight the match of zebrafish larvae with the expanding toolbox of fluorescent probes that monitor cell identity, fate and/or physiology in real time.
The review states that larval zebrafish are well matched to fluorescent probes for real-time monitoring of cell identity, fate, and physiology.
We highlight the match of zebrafish larvae with the expanding toolbox of fluorescent probes that monitor cell identity, fate and/or physiology in real time.
The review states that larval zebrafish are well matched to fluorescent probes for real-time monitoring of cell identity, fate, and physiology.
We highlight the match of zebrafish larvae with the expanding toolbox of fluorescent probes that monitor cell identity, fate and/or physiology in real time.
The review states that larval zebrafish are well matched to fluorescent probes for real-time monitoring of cell identity, fate, and physiology.
We highlight the match of zebrafish larvae with the expanding toolbox of fluorescent probes that monitor cell identity, fate and/or physiology in real time.
The review positions living larval zebrafish as a powerful translational research tool and forecasts replacement of many cell line-based studies for understanding organ pathophysiology in whole organisms.
These developments make the zebrafish larvae an extremely powerful research tool for translational research. We foresee that living larval zebrafish models will replace many cell line-based studies in understanding the contribution of molecules, organelles and cells to organ pathophysiology in whole organisms.
The review positions living larval zebrafish as a powerful translational research tool and forecasts replacement of many cell line-based studies for understanding organ pathophysiology in whole organisms.
These developments make the zebrafish larvae an extremely powerful research tool for translational research. We foresee that living larval zebrafish models will replace many cell line-based studies in understanding the contribution of molecules, organelles and cells to organ pathophysiology in whole organisms.
The review positions living larval zebrafish as a powerful translational research tool and forecasts replacement of many cell line-based studies for understanding organ pathophysiology in whole organisms.
These developments make the zebrafish larvae an extremely powerful research tool for translational research. We foresee that living larval zebrafish models will replace many cell line-based studies in understanding the contribution of molecules, organelles and cells to organ pathophysiology in whole organisms.
The review positions living larval zebrafish as a powerful translational research tool and forecasts replacement of many cell line-based studies for understanding organ pathophysiology in whole organisms.
These developments make the zebrafish larvae an extremely powerful research tool for translational research. We foresee that living larval zebrafish models will replace many cell line-based studies in understanding the contribution of molecules, organelles and cells to organ pathophysiology in whole organisms.
The review positions living larval zebrafish as a powerful translational research tool and forecasts replacement of many cell line-based studies for understanding organ pathophysiology in whole organisms.
These developments make the zebrafish larvae an extremely powerful research tool for translational research. We foresee that living larval zebrafish models will replace many cell line-based studies in understanding the contribution of molecules, organelles and cells to organ pathophysiology in whole organisms.
The review positions living larval zebrafish as a powerful translational research tool and forecasts replacement of many cell line-based studies for understanding organ pathophysiology in whole organisms.
These developments make the zebrafish larvae an extremely powerful research tool for translational research. We foresee that living larval zebrafish models will replace many cell line-based studies in understanding the contribution of molecules, organelles and cells to organ pathophysiology in whole organisms.
The review positions living larval zebrafish as a powerful translational research tool and forecasts replacement of many cell line-based studies for understanding organ pathophysiology in whole organisms.
These developments make the zebrafish larvae an extremely powerful research tool for translational research. We foresee that living larval zebrafish models will replace many cell line-based studies in understanding the contribution of molecules, organelles and cells to organ pathophysiology in whole organisms.
Approval Evidence
1 source2 linked approval claimsfirst-pass slug fluorescent-probes
We highlight the match of zebrafish larvae with the expanding toolbox of fluorescent probes that monitor cell identity, fate and/or physiology in real time.
Source:
toolkit fit summarysupports
The review states that larval zebrafish are well matched to fluorescent probes for real-time monitoring of cell identity, fate, and physiology.
We highlight the match of zebrafish larvae with the expanding toolbox of fluorescent probes that monitor cell identity, fate and/or physiology in real time.
Source:
translational positioningsupports
The review positions living larval zebrafish as a powerful translational research tool and forecasts replacement of many cell line-based studies for understanding organ pathophysiology in whole organisms.
These developments make the zebrafish larvae an extremely powerful research tool for translational research. We foresee that living larval zebrafish models will replace many cell line-based studies in understanding the contribution of molecules, organelles and cells to organ pathophysiology in whole organisms.
Source:
Comparisons
Source-backed strengths
A key strength is compatibility with larval zebrafish, which enable in vivo microscopy in living animals. The review specifically highlights real-time readout of cell identity, fate, and physiology, indicating utility for dynamic rather than endpoint analysis.
Compared with bisulfite amplicons
fluorescent probes and bisulfite amplicons address a similar problem space because they share translation.
Shared frame: same top-level item type; shared target processes: translation; shared mechanisms: translation_control
Compared with single-cell RNA sequencing
fluorescent probes and single-cell RNA sequencing address a similar problem space because they share translation.
Shared frame: same top-level item type; shared target processes: translation; shared mechanisms: translation_control
Relative tradeoffs: appears more independently replicated; looks easier to implement in practice.
Compared with synaptic positron emission tomography imaging
fluorescent probes and synaptic positron emission tomography imaging address a similar problem space because they share translation.
Shared frame: same top-level item type; shared target processes: translation; shared mechanisms: translation_control
Ranked Citations
- 1.