Source 1review2016Current Opinion in Pharmacology
Toolkit/fluorescent protein based reporters and biosensors
fluorescent protein based reporters and biosensors
Also known as: biosensors, fluorescent protein-based reporters
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Fluorescent protein-based reporters and biosensors are presented as optical tools for observing signaling states relevant to neuromodulatory GPCR pathways.
Usefulness & Problems
Why this is useful
Fluorescent protein-based reporters and biosensors are presented as optical tools for observing signaling states relevant to neuromodulatory GPCR pathways.; monitoring GPCR signaling; investigating neuromodulatory signaling in vitro and in vivo
Source:
Fluorescent protein-based reporters and biosensors are presented as optical tools for observing signaling states relevant to neuromodulatory GPCR pathways.
Source:
monitoring GPCR signaling
Source:
investigating neuromodulatory signaling in vitro and in vivo
Problem solved
They provide a way to read out signaling activity rather than only perturb it.; providing optical readouts of signaling pathway activity
Source:
They provide a way to read out signaling activity rather than only perturb it.
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providing optical readouts of signaling pathway activity
Problem links
providing optical readouts of signaling pathway activity
LiteratureThey provide a way to read out signaling activity rather than only perturb it.
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They provide a way to read out signaling activity rather than only perturb it.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Mechanisms
fluorescence-based optical reportingTarget processes
recombinationsignalingInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: payload burdenimplementation constraint: spectral hardware requirementoperating role: sensor
They require reporter or biosensor expression and optical imaging instrumentation; the review specifically mentions two-photon microscopy as a relevant enabling technology.; requires fluorescent reporter or biosensor expression and compatible imaging
The abstract does not indicate which reporters are best for specific pathway nodes or whether they directly manipulate signaling.; the abstract does not specify analytes, kinetics, or sensitivity limits
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
These optical techniques targeting specific members of the GPCR signaling pathway provide a broad base for investigating GPCR signaling in behavior and disease states and may support therapeutic development.
These emerging techniques targeting specific members of the GPCR signaling pathway offer an expansive base for investigating GPCR signaling in behavior and disease states, in addition to paving a path to potential therapeutic developments.
Optogenetics provides means to control cell signaling with spatiotemporal control in discrete cell types.
Optogenetics has revolutionized neuroscience by providing means to control cell signaling with spatiotemporal control in discrete cell types.
Cre-dependent viral vector expression and two-photon microscopy are highlighted as technologies to utilize these optical tools in vitro and in vivo.
we highlight technologies to utilize these tools in vitro and in vivo, including Cre dependent viral vector expression and two-photon microscopy
The review organizes optical manipulation of neuromodulatory GPCR signaling into four major tool classes: opsins including engineered chimeric receptors, photoactivatable proteins, photopharmacology using caged or photoswitchable molecules, and fluorescent protein-based reporters and biosensors.
we summarize four major classes of optical tools to manipulate neuromodulatory GPCR signaling: opsins (including engineered chimeric receptors); photoactivatable proteins; photopharmacology through caging-photoswitchable molecules; fluorescent protein based reporters and biosensors
Approval Evidence
1 source3 linked approval claimsfirst-pass slug fluorescent-protein-based-reporters-and-biosensors
fluorescent protein based reporters and biosensors
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application scopesupports
These optical techniques targeting specific members of the GPCR signaling pathway provide a broad base for investigating GPCR signaling in behavior and disease states and may support therapeutic development.
These emerging techniques targeting specific members of the GPCR signaling pathway offer an expansive base for investigating GPCR signaling in behavior and disease states, in addition to paving a path to potential therapeutic developments.
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capability summarysupports
Optogenetics provides means to control cell signaling with spatiotemporal control in discrete cell types.
Optogenetics has revolutionized neuroscience by providing means to control cell signaling with spatiotemporal control in discrete cell types.
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review scope summarysupports
The review organizes optical manipulation of neuromodulatory GPCR signaling into four major tool classes: opsins including engineered chimeric receptors, photoactivatable proteins, photopharmacology using caged or photoswitchable molecules, and fluorescent protein-based reporters and biosensors.
we summarize four major classes of optical tools to manipulate neuromodulatory GPCR signaling: opsins (including engineered chimeric receptors); photoactivatable proteins; photopharmacology through caging-photoswitchable molecules; fluorescent protein based reporters and biosensors
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Comparisons
Source-stated alternatives
They complement actuator-oriented classes such as opsins, engineered chimeric receptors, photoactivatable proteins, and photopharmacology.
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They complement actuator-oriented classes such as opsins, engineered chimeric receptors, photoactivatable proteins, and photopharmacology.
Source-backed strengths
included as a major optical tool class for GPCR pathway investigation
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included as a major optical tool class for GPCR pathway investigation
Compared with opsins
They complement actuator-oriented classes such as opsins, engineered chimeric receptors, photoactivatable proteins, and photopharmacology.
Shared frame: source-stated alternative in extracted literature
Strengths here: included as a major optical tool class for GPCR pathway investigation.
Relative tradeoffs: the abstract does not specify analytes, kinetics, or sensitivity limits.
Source:
They complement actuator-oriented classes such as opsins, engineered chimeric receptors, photoactivatable proteins, and photopharmacology.
Ranked Citations
- 1.