Source 1primary paper2025MED
Toolkit/FR-GECO1a
FR-GECO1a
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Here we report the design and development of two far-red fluorescent GECIs, FR-GECO1a and FR-GECO1c, based on the monomeric far-red fluorescent proteins mKelly1 and mKelly2.
Usefulness & Problems
Why this is useful
FR-GECO1a is a far-red fluorescent genetically encoded calcium indicator for imaging Ca2+ dynamics and neuronal activity. The abstract reports in vitro calcium-responsive fluorescence change and neuronal single-action-potential detection.; functional imaging of Ca2+ dynamics; neuronal activity imaging; far-red calcium sensing
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FR-GECO1a is a far-red fluorescent genetically encoded calcium indicator for imaging Ca2+ dynamics and neuronal activity. The abstract reports in vitro calcium-responsive fluorescence change and neuronal single-action-potential detection.
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functional imaging of Ca2+ dynamics
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neuronal activity imaging
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far-red calcium sensing
Problem solved
It addresses the need for far-red calcium indicators for functional imaging and all-optical activity measurement. The far-red spectral window is presented as compatible with optogenetic actuator combinations.; provides a far-red genetically encoded calcium indicator; supports calcium readout compatible with optogenetic actuator combinations
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It addresses the need for far-red calcium indicators for functional imaging and all-optical activity measurement. The far-red spectral window is presented as compatible with optogenetic actuator combinations.
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provides a far-red genetically encoded calcium indicator
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supports calcium readout compatible with optogenetic actuator combinations
Problem links
provides a far-red genetically encoded calcium indicator
LiteratureIt addresses the need for far-red calcium indicators for functional imaging and all-optical activity measurement. The far-red spectral window is presented as compatible with optogenetic actuator combinations.
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It addresses the need for far-red calcium indicators for functional imaging and all-optical activity measurement. The far-red spectral window is presented as compatible with optogenetic actuator combinations.
supports calcium readout compatible with optogenetic actuator combinations
LiteratureIt addresses the need for far-red calcium indicators for functional imaging and all-optical activity measurement. The far-red spectral window is presented as compatible with optogenetic actuator combinations.
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It addresses the need for far-red calcium indicators for functional imaging and all-optical activity measurement. The far-red spectral window is presented as compatible with optogenetic actuator combinations.
Published Workflows
Objective: Design and develop far-red fluorescent genetically encoded calcium indicators for functional imaging of calcium dynamics and neuronal activity, including compatibility with in vivo all-optical manipulation and measurement.
Why it works: The abstract frames the campaign as building new GECIs from monomeric far-red fluorescent protein scaffolds and evaluating spectral, in vitro calcium-response, brightness, affinity, neuronal detection, and in vivo all-optical compatibility properties.
calcium-dependent fluorescence reportingprotein indicator design and development
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Mechanisms
calcium-dependent fluorescent sensingTechniques
Computational DesignTarget processes
No target processes tagged yet.
Input: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: sensor
Use requires expression of the genetically encoded indicator in cells or tissue and fluorescence imaging under one-photon or two-photon illumination. The abstract also frames use alongside optogenetic actuators for all-optical experiments.; is based on monomeric far-red fluorescent protein scaffolds mKelly1 and mKelly2
Needs compatible illumination hardware and optical access. Independent follow-up evidence is still limited. Validation breadth across biological contexts is still narrow. Independent reuse still looks limited, so the evidence base may be fragile. No canonical validation observations are stored yet, so context-specific performance remains under-specified.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
FR-GECO indicators enable in vivo all-optical manipulation and measurement of cellular activities in combination with optogenetic actuators.
FR-GECO1a and FR-GECO1c have high apparent calcium affinities.
apparent Kd 29 nMapparent Kd 83 nM
FR-GECO indicators are bright under both one-photon and two-photon illumination.
FR-GECO1a and FR-GECO1c display large in vitro fluorescence responses to calcium.
deltaF/F0 6deltaF/F0 18
FR-GECO indicators offer sensitive and fast detection of single action potentials in neurons.
FR-GECO indicators have excitation and emission maxima at approximately 596 nm and 644 nm, respectively.
emission maximum 644 nmexcitation maximum 596 nm
This paper reports the design and development of two far-red fluorescent genetically encoded calcium indicators, FR-GECO1a and FR-GECO1c.
Approval Evidence
1 source7 linked approval claimsfirst-pass slug fr-geco1a
Here we report the design and development of two far-red fluorescent GECIs, FR-GECO1a and FR-GECO1c, based on the monomeric far-red fluorescent proteins mKelly1 and mKelly2.
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all optical compatibilitysupports
FR-GECO indicators enable in vivo all-optical manipulation and measurement of cellular activities in combination with optogenetic actuators.
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binding affinitysupports
FR-GECO1a and FR-GECO1c have high apparent calcium affinities.
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illumination compatibilitysupports
FR-GECO indicators are bright under both one-photon and two-photon illumination.
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in vitro performancesupports
FR-GECO1a and FR-GECO1c display large in vitro fluorescence responses to calcium.
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neuronal activity detectionsupports
FR-GECO indicators offer sensitive and fast detection of single action potentials in neurons.
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spectral propertysupports
FR-GECO indicators have excitation and emission maxima at approximately 596 nm and 644 nm, respectively.
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tool developmentsupports
This paper reports the design and development of two far-red fluorescent genetically encoded calcium indicators, FR-GECO1a and FR-GECO1c.
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Comparisons
Source-stated alternatives
The web research summary states that the anchor study explicitly benchmarks FR-GECO variants against jRGECO1a, jRCaMP1a, and near-infrared GECIs.
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The web research summary states that the anchor study explicitly benchmarks FR-GECO variants against jRGECO1a, jRCaMP1a, and near-infrared GECIs.
Source-backed strengths
large in vitro Ca2+ response; bright under one-photon and two-photon illumination; high apparent Ca2+ affinity; supports sensitive and fast detection of single action potentials in neurons
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large in vitro Ca2+ response
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bright under one-photon and two-photon illumination
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high apparent Ca2+ affinity
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supports sensitive and fast detection of single action potentials in neurons
Compared with genetically encoded Ca2+ indicators
The web research summary states that the anchor study explicitly benchmarks FR-GECO variants against jRGECO1a, jRCaMP1a, and near-infrared GECIs.
Shared frame: source-stated alternative in extracted literature
Strengths here: large in vitro Ca2+ response; bright under one-photon and two-photon illumination; high apparent Ca2+ affinity.
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The web research summary states that the anchor study explicitly benchmarks FR-GECO variants against jRGECO1a, jRCaMP1a, and near-infrared GECIs.
Compared with genetically encoded calcium indicators
The web research summary states that the anchor study explicitly benchmarks FR-GECO variants against jRGECO1a, jRCaMP1a, and near-infrared GECIs.
Shared frame: source-stated alternative in extracted literature
Strengths here: large in vitro Ca2+ response; bright under one-photon and two-photon illumination; high apparent Ca2+ affinity.
Source:
The web research summary states that the anchor study explicitly benchmarks FR-GECO variants against jRGECO1a, jRCaMP1a, and near-infrared GECIs.
Ranked Citations
- 1.