Source 1primary paper2022Frontiers in Bioengineering and Biotechnology
Toolkit/FUN-LOV
FUN-LOV
Also known as: FUNgal Light Oxygen and Voltage, FUNgal-Light Oxygen Voltage, fungal light-oxygen-voltage system
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
FUN-LOV is a fungal light-oxygen-voltage optogenetic switch for yeast built from Neurospora crassa photoreceptors WC-1 and VVD. It uses the photon-regulated interaction of these components to drive light-dependent target gene activation, including GPD1 and ADH1 expression in a wine yeast strain.
Usefulness & Problems
Why this is useful
FUN-LOV provides optical control of gene expression in yeast and has been applied to regulate fermentation-associated genes in a light-dependent manner. The cited application indicates that it can alter the balance of metabolic products of interest during wine fermentation.
Source:
Optogenetic tools can control yeast fermentation in a wine yeast strain and change the balance of metabolic products of interest in a light-dependent manner.
Source:
Altogether, the new FUN-LOV variants described here are functional in different yeast strains, expanding the biotechnological applications of this optogenetic tool.
Source:
In the budding yeast Saccharomyces cerevisiae, the FUN-LOV (FUNgal Light Oxygen and Voltage) optogenetic switch enables high levels of light-activated gene expression in a reversible and tunable fashion.
Source:
the main optogenetic systems implemented in the budding yeast Saccharomyces cerevisiae, which allow orthogonal control (by light) of gene expression, protein subcellular localization, reconstitution of protein activity, and protein sequestration by oligomerization
Problem solved
FUN-LOV addresses the need for externally controllable, light-responsive regulation of yeast gene expression during biotechnology applications. In the supplied evidence, it was used to control GPD1 and ADH1 expression and thereby modulate fermentation outputs in a wine yeast context.
Source:
Optogenetic tools can control yeast fermentation in a wine yeast strain and change the balance of metabolic products of interest in a light-dependent manner.
Source:
Altogether, the new FUN-LOV variants described here are functional in different yeast strains, expanding the biotechnological applications of this optogenetic tool.
Source:
Light-controlled expression of the flocculin encoding gene FLO1, by the FUN-LOV switch, yielded Flocculation in Light (FIL), whereas the light-controlled expression of the co-repressor TUP1 provided Flocculation in Darkness (FID).
Published Workflows
Objective: Engineer a wine yeast strain with light-responsive control of ADH1 and GPD1 to alter fermentation metabolite balance, especially glycerol production, during fermentation.
Why it works: The workflow first verifies that light changes target-gene expression, then tests whether those expression changes produce measurable fermentation phenotypes and metabolite shifts under different illumination conditions.
optogenetic regulation of ADH1 expressionoptogenetic regulation of GPD1 expressionoptogenetic controlRT-qPCRtranslational reporter assaygrowth curve assaylaboratory-scale fermentationfermentation time-course metabolite monitoring
Stages
- 1.Initial confirmation of light-controlled target-gene expression(functional_characterization)
This stage establishes that the optogenetic system actually controls the intended target genes before downstream phenotype testing.
Selection: Evidence that illumination changes expression of GPD1 and ADH1 in engineered strains.
- 2.Phenotypic characterization under different illumination conditions(secondary_characterization)
This stage tests whether light-controlled gene expression produces observable strain-level phenotypes in relevant fermentation assays.
Selection: Phenotypic differences between illumination conditions in growth and laboratory-scale fermentation assays.
- 3.Fermentation time-course metabolite monitoring(confirmatory_validation)
This stage directly measures whether optogenetic control changes fermentation outputs of interest, especially glycerol and ethanol.
Selection: Changes in glucose consumption and ethanol and glycerol production over fermentation time under different illumination conditions.
Steps
- 1.Confirm light-controlled GPD1 expression by RT-qPCRoptogenetic regulator being tested
Verify that the engineered strain shows light-responsive control of GPD1 expression.
The abstract states this confirmation was done initially, before broader phenotypic characterization.
- 2.Confirm light-controlled ADH1 expression with a translational reporteroptogenetic regulator being tested
Verify that the engineered strain shows light-responsive control of ADH1 expression.
This confirmation is described as part of the initial validation before phenotype assays.
- 3.Perform growth curve assays under different illumination conditionsengineered optogenetic system under characterization
Assess whether light-controlled target-gene expression produces growth phenotypes.
After confirming gene-expression control, the authors characterized the generated strains phenotypically.
- 4.Perform laboratory-scale fermentations under different illumination conditionsengineered optogenetic system under characterization
Test whether optogenetic control affects fermentation phenotypes in a relevant assay format.
The abstract places laboratory-scale fermentation after initial expression confirmation as part of strain characterization.
- 5.Monitor glucose consumption and ethanol and glycerol production during fermentation time courseengineered optogenetic system under confirmatory metabolic testing
Directly quantify whether optogenetic control changes fermentation metabolite outputs over time.
This follows expression and phenotype characterization to connect optogenetic control to the final metabolic outputs of interest.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
light-induced protein-protein interactionMembrane RecruitmentOligomerizationtranscriptional activationTechniques
No technique tags yet.
Target processes
localizationrecombinationtranscriptionInput: Light
Implementation Constraints
FUN-LOV is described as a multi-component switch based on Neurospora crassa photoreceptors WC-1 and VVD and their photon-regulated interaction. The evidence further indicates modular optimization in yeast and implementation for light-controlled expression of GPD1 and ADH1 in a wine yeast strain, but construct architecture and cofactor requirements are not specified in the supplied text.
The supplied evidence does not provide quantitative performance metrics, illumination parameters, dynamic range, leakiness, or response kinetics. It also does not document validation outside yeast or establish how broadly the system generalizes across strains and promoters.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Observations
successYeastapplication demoSaccharomyces cerevisiae
luciferase reporter
Inferred from claim c2 during normalization. In yeast, FUN-LOV enables light-controlled gene expression with over 1300-fold dynamic range measured by a luciferase reporter. Derived from claim c2. Quoted text: When tested in yeast, FUN-LOV yields light-controlled gene expression with exquisite temporal resolution, and a broad dynamic range of over 1300-fold, as measured by a luciferase reporter.
Source:
dynamic range1300(>)
successYeastapplication demoSaccharomyces cerevisiae
Western blot
Inferred from claim c3 during normalization. In Saccharomyces cerevisiae, FUN-LOV-driven heterologous protein expression under light stimulation exceeded expression from a classic GAL4/galactose inducible system by 2.5-fold. Derived from claim c3. Quoted text: Western blot analysis confirmed strong induction upon light stimulation, surpassing by 2.5 times the levels achieved with a classic GAL4 /galactose chemical inducible system.
Source:
expression level relative to GAL4/galactose system2.5 fold(=)
successYeastapplication demo
Inferred from claim c4 during normalization. FUN-LOV can control yeast flocculation through light-regulated expression programs, producing Flocculation in Light via FLO1 expression and Flocculation in Darkness via TUP1 expression. Derived from claim c4. Quoted text: Light-controlled expression of the flocculin encoding gene FLO1, by the FUN-LOV switch, yielded Flocculation in Light (FIL), whereas the light-controlled expression of the co-repressor TUP1 provided Flocculation in Darkness (FID).
Source:
successYeastapplication demo
Inferred from claim c5 during normalization. In yeast cells, FUN-LOV allowed tight regulation of gene expression with low background in darkness and potent control by light. Derived from claim c5. Quoted text: In yeast cells, FUN-LOV allowed tight regulation of gene expression, with low background in darkness and a highly dynamic and potent control by light.
Source:
successYeastapplication demoSaccharomyces cerevisiae
luciferase reporter
Inferred from claim c2 during normalization. In yeast, FUN-LOV enables light-controlled gene expression with over 1300-fold dynamic range measured by a luciferase reporter. Derived from claim c2. Quoted text: When tested in yeast, FUN-LOV yields light-controlled gene expression with exquisite temporal resolution, and a broad dynamic range of over 1300-fold, as measured by a luciferase reporter.
Source:
dynamic range1300(>)
successYeastapplication demoSaccharomyces cerevisiae
Western blot
Inferred from claim c3 during normalization. In Saccharomyces cerevisiae, FUN-LOV-driven heterologous protein expression under light stimulation exceeded expression from a classic GAL4/galactose inducible system by 2.5-fold. Derived from claim c3. Quoted text: Western blot analysis confirmed strong induction upon light stimulation, surpassing by 2.5 times the levels achieved with a classic GAL4 /galactose chemical inducible system.
Source:
expression level relative to GAL4/galactose system2.5 fold(=)
successYeastapplication demo
Inferred from claim c4 during normalization. FUN-LOV can control yeast flocculation through light-regulated expression programs, producing Flocculation in Light via FLO1 expression and Flocculation in Darkness via TUP1 expression. Derived from claim c4. Quoted text: Light-controlled expression of the flocculin encoding gene FLO1, by the FUN-LOV switch, yielded Flocculation in Light (FIL), whereas the light-controlled expression of the co-repressor TUP1 provided Flocculation in Darkness (FID).
Source:
successYeastapplication demo
Inferred from claim c5 during normalization. In yeast cells, FUN-LOV allowed tight regulation of gene expression with low background in darkness and potent control by light. Derived from claim c5. Quoted text: In yeast cells, FUN-LOV allowed tight regulation of gene expression, with low background in darkness and a highly dynamic and potent control by light.
Source:
successYeastapplication demoSaccharomyces cerevisiae
luciferase reporter
Inferred from claim c2 during normalization. In yeast, FUN-LOV enables light-controlled gene expression with over 1300-fold dynamic range measured by a luciferase reporter. Derived from claim c2. Quoted text: When tested in yeast, FUN-LOV yields light-controlled gene expression with exquisite temporal resolution, and a broad dynamic range of over 1300-fold, as measured by a luciferase reporter.
Source:
dynamic range1300(>)
successYeastapplication demoSaccharomyces cerevisiae
Western blot
Inferred from claim c3 during normalization. In Saccharomyces cerevisiae, FUN-LOV-driven heterologous protein expression under light stimulation exceeded expression from a classic GAL4/galactose inducible system by 2.5-fold. Derived from claim c3. Quoted text: Western blot analysis confirmed strong induction upon light stimulation, surpassing by 2.5 times the levels achieved with a classic GAL4 /galactose chemical inducible system.
Source:
expression level relative to GAL4/galactose system2.5 fold(=)
successYeastapplication demo
Inferred from claim c4 during normalization. FUN-LOV can control yeast flocculation through light-regulated expression programs, producing Flocculation in Light via FLO1 expression and Flocculation in Darkness via TUP1 expression. Derived from claim c4. Quoted text: Light-controlled expression of the flocculin encoding gene FLO1, by the FUN-LOV switch, yielded Flocculation in Light (FIL), whereas the light-controlled expression of the co-repressor TUP1 provided Flocculation in Darkness (FID).
Source:
successYeastapplication demo
Inferred from claim c5 during normalization. In yeast cells, FUN-LOV allowed tight regulation of gene expression with low background in darkness and potent control by light. Derived from claim c5. Quoted text: In yeast cells, FUN-LOV allowed tight regulation of gene expression, with low background in darkness and a highly dynamic and potent control by light.
Source:
successYeastapplication demoSaccharomyces cerevisiae
luciferase reporter
Inferred from claim c2 during normalization. In yeast, FUN-LOV enables light-controlled gene expression with over 1300-fold dynamic range measured by a luciferase reporter. Derived from claim c2. Quoted text: When tested in yeast, FUN-LOV yields light-controlled gene expression with exquisite temporal resolution, and a broad dynamic range of over 1300-fold, as measured by a luciferase reporter.
Source:
dynamic range1300(>)
successYeastapplication demoSaccharomyces cerevisiae
Western blot
Inferred from claim c3 during normalization. In Saccharomyces cerevisiae, FUN-LOV-driven heterologous protein expression under light stimulation exceeded expression from a classic GAL4/galactose inducible system by 2.5-fold. Derived from claim c3. Quoted text: Western blot analysis confirmed strong induction upon light stimulation, surpassing by 2.5 times the levels achieved with a classic GAL4 /galactose chemical inducible system.
Source:
expression level relative to GAL4/galactose system2.5 fold(=)
successYeastapplication demo
Inferred from claim c4 during normalization. FUN-LOV can control yeast flocculation through light-regulated expression programs, producing Flocculation in Light via FLO1 expression and Flocculation in Darkness via TUP1 expression. Derived from claim c4. Quoted text: Light-controlled expression of the flocculin encoding gene FLO1, by the FUN-LOV switch, yielded Flocculation in Light (FIL), whereas the light-controlled expression of the co-repressor TUP1 provided Flocculation in Darkness (FID).
Source:
successYeastapplication demo
Inferred from claim c5 during normalization. In yeast cells, FUN-LOV allowed tight regulation of gene expression with low background in darkness and potent control by light. Derived from claim c5. Quoted text: In yeast cells, FUN-LOV allowed tight regulation of gene expression, with low background in darkness and a highly dynamic and potent control by light.
Source:
successYeastapplication demoSaccharomyces cerevisiae
luciferase reporter
Inferred from claim c2 during normalization. In yeast, FUN-LOV enables light-controlled gene expression with over 1300-fold dynamic range measured by a luciferase reporter. Derived from claim c2. Quoted text: When tested in yeast, FUN-LOV yields light-controlled gene expression with exquisite temporal resolution, and a broad dynamic range of over 1300-fold, as measured by a luciferase reporter.
Source:
dynamic range1300(>)
successYeastapplication demoSaccharomyces cerevisiae
Western blot
Inferred from claim c3 during normalization. In Saccharomyces cerevisiae, FUN-LOV-driven heterologous protein expression under light stimulation exceeded expression from a classic GAL4/galactose inducible system by 2.5-fold. Derived from claim c3. Quoted text: Western blot analysis confirmed strong induction upon light stimulation, surpassing by 2.5 times the levels achieved with a classic GAL4 /galactose chemical inducible system.
Source:
expression level relative to GAL4/galactose system2.5 fold(=)
successYeastapplication demo
Inferred from claim c4 during normalization. FUN-LOV can control yeast flocculation through light-regulated expression programs, producing Flocculation in Light via FLO1 expression and Flocculation in Darkness via TUP1 expression. Derived from claim c4. Quoted text: Light-controlled expression of the flocculin encoding gene FLO1, by the FUN-LOV switch, yielded Flocculation in Light (FIL), whereas the light-controlled expression of the co-repressor TUP1 provided Flocculation in Darkness (FID).
Source:
successYeastapplication demo
Inferred from claim c5 during normalization. In yeast cells, FUN-LOV allowed tight regulation of gene expression with low background in darkness and potent control by light. Derived from claim c5. Quoted text: In yeast cells, FUN-LOV allowed tight regulation of gene expression, with low background in darkness and a highly dynamic and potent control by light.
Source:
successYeastapplication demoSaccharomyces cerevisiae
luciferase reporter
Inferred from claim c2 during normalization. In yeast, FUN-LOV enables light-controlled gene expression with over 1300-fold dynamic range measured by a luciferase reporter. Derived from claim c2. Quoted text: When tested in yeast, FUN-LOV yields light-controlled gene expression with exquisite temporal resolution, and a broad dynamic range of over 1300-fold, as measured by a luciferase reporter.
Source:
dynamic range1300(>)
successYeastapplication demoSaccharomyces cerevisiae
Western blot
Inferred from claim c3 during normalization. In Saccharomyces cerevisiae, FUN-LOV-driven heterologous protein expression under light stimulation exceeded expression from a classic GAL4/galactose inducible system by 2.5-fold. Derived from claim c3. Quoted text: Western blot analysis confirmed strong induction upon light stimulation, surpassing by 2.5 times the levels achieved with a classic GAL4 /galactose chemical inducible system.
Source:
expression level relative to GAL4/galactose system2.5 fold(=)
successYeastapplication demo
Inferred from claim c4 during normalization. FUN-LOV can control yeast flocculation through light-regulated expression programs, producing Flocculation in Light via FLO1 expression and Flocculation in Darkness via TUP1 expression. Derived from claim c4. Quoted text: Light-controlled expression of the flocculin encoding gene FLO1, by the FUN-LOV switch, yielded Flocculation in Light (FIL), whereas the light-controlled expression of the co-repressor TUP1 provided Flocculation in Darkness (FID).
Source:
successYeastapplication demo
Inferred from claim c5 during normalization. In yeast cells, FUN-LOV allowed tight regulation of gene expression with low background in darkness and potent control by light. Derived from claim c5. Quoted text: In yeast cells, FUN-LOV allowed tight regulation of gene expression, with low background in darkness and a highly dynamic and potent control by light.
Source:
successYeastapplication demoSaccharomyces cerevisiae
luciferase reporter
Inferred from claim c2 during normalization. In yeast, FUN-LOV enables light-controlled gene expression with over 1300-fold dynamic range measured by a luciferase reporter. Derived from claim c2. Quoted text: When tested in yeast, FUN-LOV yields light-controlled gene expression with exquisite temporal resolution, and a broad dynamic range of over 1300-fold, as measured by a luciferase reporter.
Source:
dynamic range1300(>)
successYeastapplication demoSaccharomyces cerevisiae
Western blot
Inferred from claim c3 during normalization. In Saccharomyces cerevisiae, FUN-LOV-driven heterologous protein expression under light stimulation exceeded expression from a classic GAL4/galactose inducible system by 2.5-fold. Derived from claim c3. Quoted text: Western blot analysis confirmed strong induction upon light stimulation, surpassing by 2.5 times the levels achieved with a classic GAL4 /galactose chemical inducible system.
Source:
expression level relative to GAL4/galactose system2.5 fold(=)
successYeastapplication demo
Inferred from claim c4 during normalization. FUN-LOV can control yeast flocculation through light-regulated expression programs, producing Flocculation in Light via FLO1 expression and Flocculation in Darkness via TUP1 expression. Derived from claim c4. Quoted text: Light-controlled expression of the flocculin encoding gene FLO1, by the FUN-LOV switch, yielded Flocculation in Light (FIL), whereas the light-controlled expression of the co-repressor TUP1 provided Flocculation in Darkness (FID).
Source:
successYeastapplication demo
Inferred from claim c5 during normalization. In yeast cells, FUN-LOV allowed tight regulation of gene expression with low background in darkness and potent control by light. Derived from claim c5. Quoted text: In yeast cells, FUN-LOV allowed tight regulation of gene expression, with low background in darkness and a highly dynamic and potent control by light.
Source:
Supporting Sources
Source 2primary paper2018
Source 3primary paper2018mBio
Source 4primary paper2021International Journal of Molecular Sciences
Source 5primary paper2025MED
Source 6primary paper2020Yeast
Ranked Claims
Optogenetic tools can control yeast fermentation in a wine yeast strain and change the balance of metabolic products of interest in a light-dependent manner.
Optogenetic tools can control yeast fermentation in a wine yeast strain and change the balance of metabolic products of interest in a light-dependent manner.
Optogenetic tools can control yeast fermentation in a wine yeast strain and change the balance of metabolic products of interest in a light-dependent manner.
Optogenetic tools can control yeast fermentation in a wine yeast strain and change the balance of metabolic products of interest in a light-dependent manner.
Optogenetic tools can control yeast fermentation in a wine yeast strain and change the balance of metabolic products of interest in a light-dependent manner.
Optogenetic tools can control yeast fermentation in a wine yeast strain and change the balance of metabolic products of interest in a light-dependent manner.
Optogenetic tools can control yeast fermentation in a wine yeast strain and change the balance of metabolic products of interest in a light-dependent manner.
Optogenetic tools can control yeast fermentation in a wine yeast strain and change the balance of metabolic products of interest in a light-dependent manner.
Engineered strains showed light-controlled expression of GPD1 and ADH1.
Engineered strains showed light-controlled expression of GPD1 and ADH1.
Engineered strains showed light-controlled expression of GPD1 and ADH1.
Engineered strains showed light-controlled expression of GPD1 and ADH1.
Engineered strains showed light-controlled expression of GPD1 and ADH1.
Engineered strains showed light-controlled expression of GPD1 and ADH1.
Engineered strains showed light-controlled expression of GPD1 and ADH1.
Engineered strains showed light-controlled expression of GPD1 and ADH1.
FUN-LOV can be used to regulate ADH1 and GPD1 expression in a wine yeast strain using light.
FUN-LOV can be used to regulate ADH1 and GPD1 expression in a wine yeast strain using light.
FUN-LOV can be used to regulate ADH1 and GPD1 expression in a wine yeast strain using light.
FUN-LOV can be used to regulate ADH1 and GPD1 expression in a wine yeast strain using light.
FUN-LOV can be used to regulate ADH1 and GPD1 expression in a wine yeast strain using light.
FUN-LOV can be used to regulate ADH1 and GPD1 expression in a wine yeast strain using light.
FUN-LOV can be used to regulate ADH1 and GPD1 expression in a wine yeast strain using light.
FUN-LOV can be used to regulate ADH1 and GPD1 expression in a wine yeast strain using light.
Optogenetic control of ADH1 showed an inverted phenotype in which glycerol production increased under constant darkness conditions.
Optogenetic control of ADH1 showed an inverted phenotype in which glycerol production increased under constant darkness conditions.
Optogenetic control of ADH1 showed an inverted phenotype in which glycerol production increased under constant darkness conditions.
Optogenetic control of ADH1 showed an inverted phenotype in which glycerol production increased under constant darkness conditions.
Optogenetic control of ADH1 showed an inverted phenotype in which glycerol production increased under constant darkness conditions.
Optogenetic control of ADH1 showed an inverted phenotype in which glycerol production increased under constant darkness conditions.
Optogenetic control of ADH1 showed an inverted phenotype in which glycerol production increased under constant darkness conditions.
Optogenetic control of ADH1 showed an inverted phenotype in which glycerol production increased under constant darkness conditions.
Optogenetic control of GPD1 increased glycerol production under constant illumination without affecting ethanol production.
Optogenetic control of GPD1 increased glycerol production under constant illumination without affecting ethanol production.
Optogenetic control of GPD1 increased glycerol production under constant illumination without affecting ethanol production.
Optogenetic control of GPD1 increased glycerol production under constant illumination without affecting ethanol production.
Optogenetic control of GPD1 increased glycerol production under constant illumination without affecting ethanol production.
Optogenetic control of GPD1 increased glycerol production under constant illumination without affecting ethanol production.
Optogenetic control of GPD1 increased glycerol production under constant illumination without affecting ethanol production.
Optogenetic control of GPD1 increased glycerol production under constant illumination without affecting ethanol production.
The new FUN-LOV variants are functional in different yeast strains and expand the biotechnological applications of the optogenetic tool.
Altogether, the new FUN-LOV variants described here are functional in different yeast strains, expanding the biotechnological applications of this optogenetic tool.
The new FUN-LOV variants are functional in different yeast strains and expand the biotechnological applications of the optogenetic tool.
Altogether, the new FUN-LOV variants described here are functional in different yeast strains, expanding the biotechnological applications of this optogenetic tool.
The new FUN-LOV variants are functional in different yeast strains and expand the biotechnological applications of the optogenetic tool.
Altogether, the new FUN-LOV variants described here are functional in different yeast strains, expanding the biotechnological applications of this optogenetic tool.
The new FUN-LOV variants are functional in different yeast strains and expand the biotechnological applications of the optogenetic tool.
Altogether, the new FUN-LOV variants described here are functional in different yeast strains, expanding the biotechnological applications of this optogenetic tool.
The new FUN-LOV variants are functional in different yeast strains and expand the biotechnological applications of the optogenetic tool.
Altogether, the new FUN-LOV variants described here are functional in different yeast strains, expanding the biotechnological applications of this optogenetic tool.
The new FUN-LOV variants are functional in different yeast strains and expand the biotechnological applications of the optogenetic tool.
Altogether, the new FUN-LOV variants described here are functional in different yeast strains, expanding the biotechnological applications of this optogenetic tool.
The new FUN-LOV variants are functional in different yeast strains and expand the biotechnological applications of the optogenetic tool.
Altogether, the new FUN-LOV variants described here are functional in different yeast strains, expanding the biotechnological applications of this optogenetic tool.
FUN-LOVSP-Nat and FUN-LOVSP-Hph reached higher luciferase expression upon blue-light stimulation than the original FUN-LOV system in BY4741 yeast, in both episomal and genome-integrated formats.
The results indicate that FUN-LOVSP-Nat and FUN-LOVSP-Hph, either episomally or genome integrated, reached higher levels of luciferase expression upon blue-light stimulation compared the original FUN-LOV system.
FUN-LOVSP-Nat and FUN-LOVSP-Hph reached higher luciferase expression upon blue-light stimulation than the original FUN-LOV system in BY4741 yeast, in both episomal and genome-integrated formats.
The results indicate that FUN-LOVSP-Nat and FUN-LOVSP-Hph, either episomally or genome integrated, reached higher levels of luciferase expression upon blue-light stimulation compared the original FUN-LOV system.
FUN-LOVSP-Nat and FUN-LOVSP-Hph reached higher luciferase expression upon blue-light stimulation than the original FUN-LOV system in BY4741 yeast, in both episomal and genome-integrated formats.
The results indicate that FUN-LOVSP-Nat and FUN-LOVSP-Hph, either episomally or genome integrated, reached higher levels of luciferase expression upon blue-light stimulation compared the original FUN-LOV system.
FUN-LOVSP-Nat and FUN-LOVSP-Hph reached higher luciferase expression upon blue-light stimulation than the original FUN-LOV system in BY4741 yeast, in both episomal and genome-integrated formats.
The results indicate that FUN-LOVSP-Nat and FUN-LOVSP-Hph, either episomally or genome integrated, reached higher levels of luciferase expression upon blue-light stimulation compared the original FUN-LOV system.
FUN-LOVSP-Nat and FUN-LOVSP-Hph reached higher luciferase expression upon blue-light stimulation than the original FUN-LOV system in BY4741 yeast, in both episomal and genome-integrated formats.
The results indicate that FUN-LOVSP-Nat and FUN-LOVSP-Hph, either episomally or genome integrated, reached higher levels of luciferase expression upon blue-light stimulation compared the original FUN-LOV system.
FUN-LOVSP-Nat and FUN-LOVSP-Hph reached higher luciferase expression upon blue-light stimulation than the original FUN-LOV system in BY4741 yeast, in both episomal and genome-integrated formats.
The results indicate that FUN-LOVSP-Nat and FUN-LOVSP-Hph, either episomally or genome integrated, reached higher levels of luciferase expression upon blue-light stimulation compared the original FUN-LOV system.
FUN-LOVSP-Nat and FUN-LOVSP-Hph reached higher luciferase expression upon blue-light stimulation than the original FUN-LOV system in BY4741 yeast, in both episomal and genome-integrated formats.
The results indicate that FUN-LOVSP-Nat and FUN-LOVSP-Hph, either episomally or genome integrated, reached higher levels of luciferase expression upon blue-light stimulation compared the original FUN-LOV system.
FUN-LOVSP-Hph was functional in the 59A-EC1118 wine yeast strain, with similar blue-light-induced reporter expression to the laboratory strain and lower luciferase background in darkness.
we demonstrated the functionality of FUN-LOVSP-Hph in the 59A-EC1118 wine yeast strain, showing similar levels of reporter gene induction under blue-light respect to the laboratory strain, and with lower luciferase expression background in darkness condition.
FUN-LOVSP-Hph was functional in the 59A-EC1118 wine yeast strain, with similar blue-light-induced reporter expression to the laboratory strain and lower luciferase background in darkness.
we demonstrated the functionality of FUN-LOVSP-Hph in the 59A-EC1118 wine yeast strain, showing similar levels of reporter gene induction under blue-light respect to the laboratory strain, and with lower luciferase expression background in darkness condition.
FUN-LOVSP-Hph was functional in the 59A-EC1118 wine yeast strain, with similar blue-light-induced reporter expression to the laboratory strain and lower luciferase background in darkness.
we demonstrated the functionality of FUN-LOVSP-Hph in the 59A-EC1118 wine yeast strain, showing similar levels of reporter gene induction under blue-light respect to the laboratory strain, and with lower luciferase expression background in darkness condition.
FUN-LOVSP-Hph was functional in the 59A-EC1118 wine yeast strain, with similar blue-light-induced reporter expression to the laboratory strain and lower luciferase background in darkness.
we demonstrated the functionality of FUN-LOVSP-Hph in the 59A-EC1118 wine yeast strain, showing similar levels of reporter gene induction under blue-light respect to the laboratory strain, and with lower luciferase expression background in darkness condition.
FUN-LOVSP-Hph was functional in the 59A-EC1118 wine yeast strain, with similar blue-light-induced reporter expression to the laboratory strain and lower luciferase background in darkness.
we demonstrated the functionality of FUN-LOVSP-Hph in the 59A-EC1118 wine yeast strain, showing similar levels of reporter gene induction under blue-light respect to the laboratory strain, and with lower luciferase expression background in darkness condition.
FUN-LOVSP-Hph was functional in the 59A-EC1118 wine yeast strain, with similar blue-light-induced reporter expression to the laboratory strain and lower luciferase background in darkness.
we demonstrated the functionality of FUN-LOVSP-Hph in the 59A-EC1118 wine yeast strain, showing similar levels of reporter gene induction under blue-light respect to the laboratory strain, and with lower luciferase expression background in darkness condition.
FUN-LOVSP-Hph was functional in the 59A-EC1118 wine yeast strain, with similar blue-light-induced reporter expression to the laboratory strain and lower luciferase background in darkness.
we demonstrated the functionality of FUN-LOVSP-Hph in the 59A-EC1118 wine yeast strain, showing similar levels of reporter gene induction under blue-light respect to the laboratory strain, and with lower luciferase expression background in darkness condition.
FUN-LOVSP is a single-plasmid variant of FUN-LOV generated by replacing promoter and terminator sequences and cloning the system into one plasmid.
Initially, we generated new variants of this system by replacing the promoter and terminator sequences and by cloning the system in a single plasmid (FUN-LOVSP).
FUN-LOVSP is a single-plasmid variant of FUN-LOV generated by replacing promoter and terminator sequences and cloning the system into one plasmid.
Initially, we generated new variants of this system by replacing the promoter and terminator sequences and by cloning the system in a single plasmid (FUN-LOVSP).
FUN-LOVSP is a single-plasmid variant of FUN-LOV generated by replacing promoter and terminator sequences and cloning the system into one plasmid.
Initially, we generated new variants of this system by replacing the promoter and terminator sequences and by cloning the system in a single plasmid (FUN-LOVSP).
FUN-LOVSP is a single-plasmid variant of FUN-LOV generated by replacing promoter and terminator sequences and cloning the system into one plasmid.
Initially, we generated new variants of this system by replacing the promoter and terminator sequences and by cloning the system in a single plasmid (FUN-LOVSP).
FUN-LOVSP is a single-plasmid variant of FUN-LOV generated by replacing promoter and terminator sequences and cloning the system into one plasmid.
Initially, we generated new variants of this system by replacing the promoter and terminator sequences and by cloning the system in a single plasmid (FUN-LOVSP).
FUN-LOVSP is a single-plasmid variant of FUN-LOV generated by replacing promoter and terminator sequences and cloning the system into one plasmid.
Initially, we generated new variants of this system by replacing the promoter and terminator sequences and by cloning the system in a single plasmid (FUN-LOVSP).
FUN-LOVSP is a single-plasmid variant of FUN-LOV generated by replacing promoter and terminator sequences and cloning the system into one plasmid.
Initially, we generated new variants of this system by replacing the promoter and terminator sequences and by cloning the system in a single plasmid (FUN-LOVSP).
FUN-LOVSP-Nat and FUN-LOVSP-Hph are FUN-LOVSP variants carrying nourseothricin or hygromycin resistance genes to allow selection after genome integration.
we included the nourseothricin (Nat) or hygromycin (Hph) antibiotic resistances genes in the new FUN-LOVSP plasmid, generating two new variants (FUN-LOVSP-Nat and FUN-LOVSP-Hph), to allow selection after genome integration.
FUN-LOVSP-Nat and FUN-LOVSP-Hph are FUN-LOVSP variants carrying nourseothricin or hygromycin resistance genes to allow selection after genome integration.
we included the nourseothricin (Nat) or hygromycin (Hph) antibiotic resistances genes in the new FUN-LOVSP plasmid, generating two new variants (FUN-LOVSP-Nat and FUN-LOVSP-Hph), to allow selection after genome integration.
FUN-LOVSP-Nat and FUN-LOVSP-Hph are FUN-LOVSP variants carrying nourseothricin or hygromycin resistance genes to allow selection after genome integration.
we included the nourseothricin (Nat) or hygromycin (Hph) antibiotic resistances genes in the new FUN-LOVSP plasmid, generating two new variants (FUN-LOVSP-Nat and FUN-LOVSP-Hph), to allow selection after genome integration.
FUN-LOVSP-Nat and FUN-LOVSP-Hph are FUN-LOVSP variants carrying nourseothricin or hygromycin resistance genes to allow selection after genome integration.
we included the nourseothricin (Nat) or hygromycin (Hph) antibiotic resistances genes in the new FUN-LOVSP plasmid, generating two new variants (FUN-LOVSP-Nat and FUN-LOVSP-Hph), to allow selection after genome integration.
FUN-LOVSP-Nat and FUN-LOVSP-Hph are FUN-LOVSP variants carrying nourseothricin or hygromycin resistance genes to allow selection after genome integration.
we included the nourseothricin (Nat) or hygromycin (Hph) antibiotic resistances genes in the new FUN-LOVSP plasmid, generating two new variants (FUN-LOVSP-Nat and FUN-LOVSP-Hph), to allow selection after genome integration.
FUN-LOVSP-Nat and FUN-LOVSP-Hph are FUN-LOVSP variants carrying nourseothricin or hygromycin resistance genes to allow selection after genome integration.
we included the nourseothricin (Nat) or hygromycin (Hph) antibiotic resistances genes in the new FUN-LOVSP plasmid, generating two new variants (FUN-LOVSP-Nat and FUN-LOVSP-Hph), to allow selection after genome integration.
FUN-LOVSP-Nat and FUN-LOVSP-Hph are FUN-LOVSP variants carrying nourseothricin or hygromycin resistance genes to allow selection after genome integration.
we included the nourseothricin (Nat) or hygromycin (Hph) antibiotic resistances genes in the new FUN-LOVSP plasmid, generating two new variants (FUN-LOVSP-Nat and FUN-LOVSP-Hph), to allow selection after genome integration.
FUN-LOV enables high levels of light-activated gene expression in Saccharomyces cerevisiae in a reversible and tunable fashion.
In the budding yeast Saccharomyces cerevisiae, the FUN-LOV (FUNgal Light Oxygen and Voltage) optogenetic switch enables high levels of light-activated gene expression in a reversible and tunable fashion.
FUN-LOV enables high levels of light-activated gene expression in Saccharomyces cerevisiae in a reversible and tunable fashion.
In the budding yeast Saccharomyces cerevisiae, the FUN-LOV (FUNgal Light Oxygen and Voltage) optogenetic switch enables high levels of light-activated gene expression in a reversible and tunable fashion.
FUN-LOV enables high levels of light-activated gene expression in Saccharomyces cerevisiae in a reversible and tunable fashion.
In the budding yeast Saccharomyces cerevisiae, the FUN-LOV (FUNgal Light Oxygen and Voltage) optogenetic switch enables high levels of light-activated gene expression in a reversible and tunable fashion.
FUN-LOV enables high levels of light-activated gene expression in Saccharomyces cerevisiae in a reversible and tunable fashion.
In the budding yeast Saccharomyces cerevisiae, the FUN-LOV (FUNgal Light Oxygen and Voltage) optogenetic switch enables high levels of light-activated gene expression in a reversible and tunable fashion.
FUN-LOV enables high levels of light-activated gene expression in Saccharomyces cerevisiae in a reversible and tunable fashion.
In the budding yeast Saccharomyces cerevisiae, the FUN-LOV (FUNgal Light Oxygen and Voltage) optogenetic switch enables high levels of light-activated gene expression in a reversible and tunable fashion.
FUN-LOV enables high levels of light-activated gene expression in Saccharomyces cerevisiae in a reversible and tunable fashion.
In the budding yeast Saccharomyces cerevisiae, the FUN-LOV (FUNgal Light Oxygen and Voltage) optogenetic switch enables high levels of light-activated gene expression in a reversible and tunable fashion.
FUN-LOV enables high levels of light-activated gene expression in Saccharomyces cerevisiae in a reversible and tunable fashion.
In the budding yeast Saccharomyces cerevisiae, the FUN-LOV (FUNgal Light Oxygen and Voltage) optogenetic switch enables high levels of light-activated gene expression in a reversible and tunable fashion.
Combining the Hap1p DNA-binding domain with either p65 or VP16 activation domains increased reporter expression relative to the original switch.
the combination of the Hap1p DBD with either p65 or VP16 activation domains also resulted in higher levels of reporter expression compared to the original switch
Combining the Hap1p DNA-binding domain with either p65 or VP16 activation domains increased reporter expression relative to the original switch.
the combination of the Hap1p DBD with either p65 or VP16 activation domains also resulted in higher levels of reporter expression compared to the original switch
Combining the Hap1p DNA-binding domain with either p65 or VP16 activation domains increased reporter expression relative to the original switch.
the combination of the Hap1p DBD with either p65 or VP16 activation domains also resulted in higher levels of reporter expression compared to the original switch
Combining the Hap1p DNA-binding domain with either p65 or VP16 activation domains increased reporter expression relative to the original switch.
the combination of the Hap1p DBD with either p65 or VP16 activation domains also resulted in higher levels of reporter expression compared to the original switch
Combining the Hap1p DNA-binding domain with either p65 or VP16 activation domains increased reporter expression relative to the original switch.
the combination of the Hap1p DBD with either p65 or VP16 activation domains also resulted in higher levels of reporter expression compared to the original switch
Combining the Hap1p DNA-binding domain with either p65 or VP16 activation domains increased reporter expression relative to the original switch.
the combination of the Hap1p DBD with either p65 or VP16 activation domains also resulted in higher levels of reporter expression compared to the original switch
Combining the Hap1p DNA-binding domain with either p65 or VP16 activation domains increased reporter expression relative to the original switch.
the combination of the Hap1p DBD with either p65 or VP16 activation domains also resulted in higher levels of reporter expression compared to the original switch
The HAP-LOV variant showed higher luciferase expression upon induction than FUN-LOV.
the variant carrying the Hap1p DBD, which we call "HAP-LOV", displayed higher levels of luciferase expression upon induction compared to FUN-LOV
The HAP-LOV variant showed higher luciferase expression upon induction than FUN-LOV.
the variant carrying the Hap1p DBD, which we call "HAP-LOV", displayed higher levels of luciferase expression upon induction compared to FUN-LOV
The HAP-LOV variant showed higher luciferase expression upon induction than FUN-LOV.
the variant carrying the Hap1p DBD, which we call "HAP-LOV", displayed higher levels of luciferase expression upon induction compared to FUN-LOV
The HAP-LOV variant showed higher luciferase expression upon induction than FUN-LOV.
the variant carrying the Hap1p DBD, which we call "HAP-LOV", displayed higher levels of luciferase expression upon induction compared to FUN-LOV
The HAP-LOV variant showed higher luciferase expression upon induction than FUN-LOV.
the variant carrying the Hap1p DBD, which we call "HAP-LOV", displayed higher levels of luciferase expression upon induction compared to FUN-LOV
The HAP-LOV variant showed higher luciferase expression upon induction than FUN-LOV.
the variant carrying the Hap1p DBD, which we call "HAP-LOV", displayed higher levels of luciferase expression upon induction compared to FUN-LOV
The HAP-LOV variant showed higher luciferase expression upon induction than FUN-LOV.
the variant carrying the Hap1p DBD, which we call "HAP-LOV", displayed higher levels of luciferase expression upon induction compared to FUN-LOV
Using low-copy plasmids and strong promoters for expression of FUN-LOV and HAP-LOV components produced a stronger response in both systems.
when low-copy plasmids and strong promoters were used, a stronger response was achieved in both systems
Using low-copy plasmids and strong promoters for expression of FUN-LOV and HAP-LOV components produced a stronger response in both systems.
when low-copy plasmids and strong promoters were used, a stronger response was achieved in both systems
Using low-copy plasmids and strong promoters for expression of FUN-LOV and HAP-LOV components produced a stronger response in both systems.
when low-copy plasmids and strong promoters were used, a stronger response was achieved in both systems
Using low-copy plasmids and strong promoters for expression of FUN-LOV and HAP-LOV components produced a stronger response in both systems.
when low-copy plasmids and strong promoters were used, a stronger response was achieved in both systems
Using low-copy plasmids and strong promoters for expression of FUN-LOV and HAP-LOV components produced a stronger response in both systems.
when low-copy plasmids and strong promoters were used, a stronger response was achieved in both systems
Using low-copy plasmids and strong promoters for expression of FUN-LOV and HAP-LOV components produced a stronger response in both systems.
when low-copy plasmids and strong promoters were used, a stronger response was achieved in both systems
Using low-copy plasmids and strong promoters for expression of FUN-LOV and HAP-LOV components produced a stronger response in both systems.
when low-copy plasmids and strong promoters were used, a stronger response was achieved in both systems
This paper reports modular optimization of the FUN-LOV optogenetic switch in Saccharomyces cerevisiae.
In Saccharomyces cerevisiae... we report on the modular optimization of the fungal light-oxygen-voltage (FUN-LOV) system
This paper reports modular optimization of the FUN-LOV optogenetic switch in Saccharomyces cerevisiae.
In Saccharomyces cerevisiae... we report on the modular optimization of the fungal light-oxygen-voltage (FUN-LOV) system
This paper reports modular optimization of the FUN-LOV optogenetic switch in Saccharomyces cerevisiae.
In Saccharomyces cerevisiae... we report on the modular optimization of the fungal light-oxygen-voltage (FUN-LOV) system
This paper reports modular optimization of the FUN-LOV optogenetic switch in Saccharomyces cerevisiae.
In Saccharomyces cerevisiae... we report on the modular optimization of the fungal light-oxygen-voltage (FUN-LOV) system
This paper reports modular optimization of the FUN-LOV optogenetic switch in Saccharomyces cerevisiae.
In Saccharomyces cerevisiae... we report on the modular optimization of the fungal light-oxygen-voltage (FUN-LOV) system
This paper reports modular optimization of the FUN-LOV optogenetic switch in Saccharomyces cerevisiae.
In Saccharomyces cerevisiae... we report on the modular optimization of the fungal light-oxygen-voltage (FUN-LOV) system
This paper reports modular optimization of the FUN-LOV optogenetic switch in Saccharomyces cerevisiae.
In Saccharomyces cerevisiae... we report on the modular optimization of the fungal light-oxygen-voltage (FUN-LOV) system
The study describes a new set of blue-light optogenetic switches carrying different protein modules that expands the available suite of optogenetic tools in yeast.
we describe a new set of blue-light optogenetic switches carrying different protein modules, which expands the available suite of optogenetic tools in yeast
The study describes a new set of blue-light optogenetic switches carrying different protein modules that expands the available suite of optogenetic tools in yeast.
we describe a new set of blue-light optogenetic switches carrying different protein modules, which expands the available suite of optogenetic tools in yeast
The study describes a new set of blue-light optogenetic switches carrying different protein modules that expands the available suite of optogenetic tools in yeast.
we describe a new set of blue-light optogenetic switches carrying different protein modules, which expands the available suite of optogenetic tools in yeast
The study describes a new set of blue-light optogenetic switches carrying different protein modules that expands the available suite of optogenetic tools in yeast.
we describe a new set of blue-light optogenetic switches carrying different protein modules, which expands the available suite of optogenetic tools in yeast
The study describes a new set of blue-light optogenetic switches carrying different protein modules that expands the available suite of optogenetic tools in yeast.
we describe a new set of blue-light optogenetic switches carrying different protein modules, which expands the available suite of optogenetic tools in yeast
The study describes a new set of blue-light optogenetic switches carrying different protein modules that expands the available suite of optogenetic tools in yeast.
we describe a new set of blue-light optogenetic switches carrying different protein modules, which expands the available suite of optogenetic tools in yeast
The study describes a new set of blue-light optogenetic switches carrying different protein modules that expands the available suite of optogenetic tools in yeast.
we describe a new set of blue-light optogenetic switches carrying different protein modules, which expands the available suite of optogenetic tools in yeast
New FUN-LOV switch variants were generated by replacing the Gal4 DNA-binding domain with nine different zinc cluster family yeast transcription factor DNA-binding domains.
We also describe new switch variants obtained by replacing the Gal4 DNA-binding domain (DBD) of FUN-LOV with nine different DBDs from yeast transcription factors of the zinc cluster family.
different DBDs tested 9
New FUN-LOV switch variants were generated by replacing the Gal4 DNA-binding domain with nine different zinc cluster family yeast transcription factor DNA-binding domains.
We also describe new switch variants obtained by replacing the Gal4 DNA-binding domain (DBD) of FUN-LOV with nine different DBDs from yeast transcription factors of the zinc cluster family.
different DBDs tested 9
New FUN-LOV switch variants were generated by replacing the Gal4 DNA-binding domain with nine different zinc cluster family yeast transcription factor DNA-binding domains.
We also describe new switch variants obtained by replacing the Gal4 DNA-binding domain (DBD) of FUN-LOV with nine different DBDs from yeast transcription factors of the zinc cluster family.
different DBDs tested 9
New FUN-LOV switch variants were generated by replacing the Gal4 DNA-binding domain with nine different zinc cluster family yeast transcription factor DNA-binding domains.
We also describe new switch variants obtained by replacing the Gal4 DNA-binding domain (DBD) of FUN-LOV with nine different DBDs from yeast transcription factors of the zinc cluster family.
different DBDs tested 9
New FUN-LOV switch variants were generated by replacing the Gal4 DNA-binding domain with nine different zinc cluster family yeast transcription factor DNA-binding domains.
We also describe new switch variants obtained by replacing the Gal4 DNA-binding domain (DBD) of FUN-LOV with nine different DBDs from yeast transcription factors of the zinc cluster family.
different DBDs tested 9
New FUN-LOV switch variants were generated by replacing the Gal4 DNA-binding domain with nine different zinc cluster family yeast transcription factor DNA-binding domains.
We also describe new switch variants obtained by replacing the Gal4 DNA-binding domain (DBD) of FUN-LOV with nine different DBDs from yeast transcription factors of the zinc cluster family.
different DBDs tested 9
New FUN-LOV switch variants were generated by replacing the Gal4 DNA-binding domain with nine different zinc cluster family yeast transcription factor DNA-binding domains.
We also describe new switch variants obtained by replacing the Gal4 DNA-binding domain (DBD) of FUN-LOV with nine different DBDs from yeast transcription factors of the zinc cluster family.
different DBDs tested 9
Optogenetic systems implemented in budding yeast Saccharomyces cerevisiae allow orthogonal light control of gene expression, protein subcellular localization, reconstitution of protein activity, and protein sequestration by oligomerization.
the main optogenetic systems implemented in the budding yeast Saccharomyces cerevisiae, which allow orthogonal control (by light) of gene expression, protein subcellular localization, reconstitution of protein activity, and protein sequestration by oligomerization
Optogenetic systems implemented in budding yeast Saccharomyces cerevisiae allow orthogonal light control of gene expression, protein subcellular localization, reconstitution of protein activity, and protein sequestration by oligomerization.
the main optogenetic systems implemented in the budding yeast Saccharomyces cerevisiae, which allow orthogonal control (by light) of gene expression, protein subcellular localization, reconstitution of protein activity, and protein sequestration by oligomerization
Optogenetic systems implemented in budding yeast Saccharomyces cerevisiae allow orthogonal light control of gene expression, protein subcellular localization, reconstitution of protein activity, and protein sequestration by oligomerization.
the main optogenetic systems implemented in the budding yeast Saccharomyces cerevisiae, which allow orthogonal control (by light) of gene expression, protein subcellular localization, reconstitution of protein activity, and protein sequestration by oligomerization
Optogenetic systems implemented in budding yeast Saccharomyces cerevisiae allow orthogonal light control of gene expression, protein subcellular localization, reconstitution of protein activity, and protein sequestration by oligomerization.
the main optogenetic systems implemented in the budding yeast Saccharomyces cerevisiae, which allow orthogonal control (by light) of gene expression, protein subcellular localization, reconstitution of protein activity, and protein sequestration by oligomerization
Optogenetic systems implemented in budding yeast Saccharomyces cerevisiae allow orthogonal light control of gene expression, protein subcellular localization, reconstitution of protein activity, and protein sequestration by oligomerization.
the main optogenetic systems implemented in the budding yeast Saccharomyces cerevisiae, which allow orthogonal control (by light) of gene expression, protein subcellular localization, reconstitution of protein activity, and protein sequestration by oligomerization
Optogenetic systems implemented in budding yeast Saccharomyces cerevisiae allow orthogonal light control of gene expression, protein subcellular localization, reconstitution of protein activity, and protein sequestration by oligomerization.
the main optogenetic systems implemented in the budding yeast Saccharomyces cerevisiae, which allow orthogonal control (by light) of gene expression, protein subcellular localization, reconstitution of protein activity, and protein sequestration by oligomerization
Optogenetic systems implemented in budding yeast Saccharomyces cerevisiae allow orthogonal light control of gene expression, protein subcellular localization, reconstitution of protein activity, and protein sequestration by oligomerization.
the main optogenetic systems implemented in the budding yeast Saccharomyces cerevisiae, which allow orthogonal control (by light) of gene expression, protein subcellular localization, reconstitution of protein activity, and protein sequestration by oligomerization
Optogenetics has been successfully implemented in yeast.
Optogenetics has been successfully implemented in yeast
Optogenetics has been successfully implemented in yeast.
Optogenetics has been successfully implemented in yeast
Optogenetics has been successfully implemented in yeast.
Optogenetics has been successfully implemented in yeast
Optogenetics has been successfully implemented in yeast.
Optogenetics has been successfully implemented in yeast
Optogenetics has been successfully implemented in yeast.
Optogenetics has been successfully implemented in yeast
Optogenetics has been successfully implemented in yeast.
Optogenetics has been successfully implemented in yeast
Optogenetics has been successfully implemented in yeast.
Optogenetics has been successfully implemented in yeast
FUN-LOV allows precise and strong activation of the target gene.
FUN-LOV, which allows precise and strong activation of the target gene
FUN-LOV allows precise and strong activation of the target gene.
FUN-LOV, which allows precise and strong activation of the target gene
FUN-LOV allows precise and strong activation of the target gene.
FUN-LOV, which allows precise and strong activation of the target gene
FUN-LOV allows precise and strong activation of the target gene.
FUN-LOV, which allows precise and strong activation of the target gene
FUN-LOV allows precise and strong activation of the target gene.
FUN-LOV, which allows precise and strong activation of the target gene
FUN-LOV allows precise and strong activation of the target gene.
FUN-LOV, which allows precise and strong activation of the target gene
FUN-LOV allows precise and strong activation of the target gene.
FUN-LOV, which allows precise and strong activation of the target gene
FUN-LOV can control yeast flocculation through light-regulated expression programs, producing Flocculation in Light via FLO1 expression and Flocculation in Darkness via TUP1 expression.
Light-controlled expression of the flocculin encoding gene FLO1, by the FUN-LOV switch, yielded Flocculation in Light (FIL), whereas the light-controlled expression of the co-repressor TUP1 provided Flocculation in Darkness (FID).
FUN-LOV can control yeast flocculation through light-regulated expression programs, producing Flocculation in Light via FLO1 expression and Flocculation in Darkness via TUP1 expression.
Light-controlled expression of the flocculin encoding gene FLO1, by the FUN-LOV switch, yielded Flocculation in Light (FIL), whereas the light-controlled expression of the co-repressor TUP1 provided Flocculation in Darkness (FID).
FUN-LOV can control yeast flocculation through light-regulated expression programs, producing Flocculation in Light via FLO1 expression and Flocculation in Darkness via TUP1 expression.
Light-controlled expression of the flocculin encoding gene FLO1, by the FUN-LOV switch, yielded Flocculation in Light (FIL), whereas the light-controlled expression of the co-repressor TUP1 provided Flocculation in Darkness (FID).
FUN-LOV can control yeast flocculation through light-regulated expression programs, producing Flocculation in Light via FLO1 expression and Flocculation in Darkness via TUP1 expression.
Light-controlled expression of the flocculin encoding gene FLO1, by the FUN-LOV switch, yielded Flocculation in Light (FIL), whereas the light-controlled expression of the co-repressor TUP1 provided Flocculation in Darkness (FID).
FUN-LOV can control yeast flocculation through light-regulated expression programs, producing Flocculation in Light via FLO1 expression and Flocculation in Darkness via TUP1 expression.
Light-controlled expression of the flocculin encoding gene FLO1, by the FUN-LOV switch, yielded Flocculation in Light (FIL), whereas the light-controlled expression of the co-repressor TUP1 provided Flocculation in Darkness (FID).
FUN-LOV can control yeast flocculation through light-regulated expression programs, producing Flocculation in Light via FLO1 expression and Flocculation in Darkness via TUP1 expression.
Light-controlled expression of the flocculin encoding gene FLO1, by the FUN-LOV switch, yielded Flocculation in Light (FIL), whereas the light-controlled expression of the co-repressor TUP1 provided Flocculation in Darkness (FID).
FUN-LOV can control yeast flocculation through light-regulated expression programs, producing Flocculation in Light via FLO1 expression and Flocculation in Darkness via TUP1 expression.
Light-controlled expression of the flocculin encoding gene FLO1, by the FUN-LOV switch, yielded Flocculation in Light (FIL), whereas the light-controlled expression of the co-repressor TUP1 provided Flocculation in Darkness (FID).
In yeast cells, FUN-LOV allowed tight regulation of gene expression with low background in darkness and potent control by light.
In yeast cells, FUN-LOV allowed tight regulation of gene expression, with low background in darkness and a highly dynamic and potent control by light.
In yeast cells, FUN-LOV allowed tight regulation of gene expression with low background in darkness and potent control by light.
In yeast cells, FUN-LOV allowed tight regulation of gene expression, with low background in darkness and a highly dynamic and potent control by light.
In yeast cells, FUN-LOV allowed tight regulation of gene expression with low background in darkness and potent control by light.
In yeast cells, FUN-LOV allowed tight regulation of gene expression, with low background in darkness and a highly dynamic and potent control by light.
In yeast cells, FUN-LOV allowed tight regulation of gene expression with low background in darkness and potent control by light.
In yeast cells, FUN-LOV allowed tight regulation of gene expression, with low background in darkness and a highly dynamic and potent control by light.
In yeast cells, FUN-LOV allowed tight regulation of gene expression with low background in darkness and potent control by light.
In yeast cells, FUN-LOV allowed tight regulation of gene expression, with low background in darkness and a highly dynamic and potent control by light.
In yeast cells, FUN-LOV allowed tight regulation of gene expression with low background in darkness and potent control by light.
In yeast cells, FUN-LOV allowed tight regulation of gene expression, with low background in darkness and a highly dynamic and potent control by light.
In yeast cells, FUN-LOV allowed tight regulation of gene expression with low background in darkness and potent control by light.
In yeast cells, FUN-LOV allowed tight regulation of gene expression, with low background in darkness and a highly dynamic and potent control by light.
In Saccharomyces cerevisiae, FUN-LOV-driven heterologous protein expression under light stimulation exceeded expression from a classic GAL4/galactose inducible system by 2.5-fold.
Western blot analysis confirmed strong induction upon light stimulation, surpassing by 2.5 times the levels achieved with a classic GAL4 /galactose chemical inducible system.
expression level relative to GAL4/galactose system 2.5 fold
In Saccharomyces cerevisiae, FUN-LOV-driven heterologous protein expression under light stimulation exceeded expression from a classic GAL4/galactose inducible system by 2.5-fold.
Western blot analysis confirmed strong induction upon light stimulation, surpassing by 2.5 times the levels achieved with a classic GAL4 /galactose chemical inducible system.
expression level relative to GAL4/galactose system 2.5 fold
In Saccharomyces cerevisiae, FUN-LOV-driven heterologous protein expression under light stimulation exceeded expression from a classic GAL4/galactose inducible system by 2.5-fold.
Western blot analysis confirmed strong induction upon light stimulation, surpassing by 2.5 times the levels achieved with a classic GAL4 /galactose chemical inducible system.
expression level relative to GAL4/galactose system 2.5 fold
In Saccharomyces cerevisiae, FUN-LOV-driven heterologous protein expression under light stimulation exceeded expression from a classic GAL4/galactose inducible system by 2.5-fold.
Western blot analysis confirmed strong induction upon light stimulation, surpassing by 2.5 times the levels achieved with a classic GAL4 /galactose chemical inducible system.
expression level relative to GAL4/galactose system 2.5 fold
In Saccharomyces cerevisiae, FUN-LOV-driven heterologous protein expression under light stimulation exceeded expression from a classic GAL4/galactose inducible system by 2.5-fold.
Western blot analysis confirmed strong induction upon light stimulation, surpassing by 2.5 times the levels achieved with a classic GAL4 /galactose chemical inducible system.
expression level relative to GAL4/galactose system 2.5 fold
In Saccharomyces cerevisiae, FUN-LOV-driven heterologous protein expression under light stimulation exceeded expression from a classic GAL4/galactose inducible system by 2.5-fold.
Western blot analysis confirmed strong induction upon light stimulation, surpassing by 2.5 times the levels achieved with a classic GAL4 /galactose chemical inducible system.
expression level relative to GAL4/galactose system 2.5 fold
In Saccharomyces cerevisiae, FUN-LOV-driven heterologous protein expression under light stimulation exceeded expression from a classic GAL4/galactose inducible system by 2.5-fold.
Western blot analysis confirmed strong induction upon light stimulation, surpassing by 2.5 times the levels achieved with a classic GAL4 /galactose chemical inducible system.
expression level relative to GAL4/galactose system 2.5 fold
In Saccharomyces cerevisiae, FUN-LOV-driven heterologous protein expression under light stimulation surpassed a classic GAL4/galactose inducible system by 2.5-fold.
Western blot analysis confirmed strong induction upon light stimulation, surpassing by 2.5 times the levels achieved with a classic GAL4/galactose chemical-inducible system.
expression level versus GAL4/galactose system 2.5 fold
In Saccharomyces cerevisiae, FUN-LOV-driven heterologous protein expression under light stimulation surpassed a classic GAL4/galactose inducible system by 2.5-fold.
Western blot analysis confirmed strong induction upon light stimulation, surpassing by 2.5 times the levels achieved with a classic GAL4/galactose chemical-inducible system.
expression level versus GAL4/galactose system 2.5 fold
In Saccharomyces cerevisiae, FUN-LOV-driven heterologous protein expression under light stimulation surpassed a classic GAL4/galactose inducible system by 2.5-fold.
Western blot analysis confirmed strong induction upon light stimulation, surpassing by 2.5 times the levels achieved with a classic GAL4/galactose chemical-inducible system.
expression level versus GAL4/galactose system 2.5 fold
In Saccharomyces cerevisiae, FUN-LOV-driven heterologous protein expression under light stimulation surpassed a classic GAL4/galactose inducible system by 2.5-fold.
Western blot analysis confirmed strong induction upon light stimulation, surpassing by 2.5 times the levels achieved with a classic GAL4/galactose chemical-inducible system.
expression level versus GAL4/galactose system 2.5 fold
In Saccharomyces cerevisiae, FUN-LOV-driven heterologous protein expression under light stimulation surpassed a classic GAL4/galactose inducible system by 2.5-fold.
Western blot analysis confirmed strong induction upon light stimulation, surpassing by 2.5 times the levels achieved with a classic GAL4/galactose chemical-inducible system.
expression level versus GAL4/galactose system 2.5 fold
In Saccharomyces cerevisiae, FUN-LOV-driven heterologous protein expression under light stimulation surpassed a classic GAL4/galactose inducible system by 2.5-fold.
Western blot analysis confirmed strong induction upon light stimulation, surpassing by 2.5 times the levels achieved with a classic GAL4/galactose chemical-inducible system.
expression level versus GAL4/galactose system 2.5 fold
In Saccharomyces cerevisiae, FUN-LOV-driven heterologous protein expression under light stimulation surpassed a classic GAL4/galactose inducible system by 2.5-fold.
Western blot analysis confirmed strong induction upon light stimulation, surpassing by 2.5 times the levels achieved with a classic GAL4/galactose chemical-inducible system.
expression level versus GAL4/galactose system 2.5 fold
FUN-LOV is an optogenetic switch based on the photon-regulated interaction of WC-1 and VVD from Neurospora crassa.
we implemented FUN-LOV, an optogenetic switch based on the photon-regulated interaction of WC-1 and VVD, two LOV (light-oxygen-voltage) blue-light photoreceptors from the fungus Neurospora crassa
FUN-LOV is an optogenetic switch based on the photon-regulated interaction of WC-1 and VVD from Neurospora crassa.
we implemented FUN-LOV, an optogenetic switch based on the photon-regulated interaction of WC-1 and VVD, two LOV (light-oxygen-voltage) blue-light photoreceptors from the fungus Neurospora crassa
FUN-LOV is an optogenetic switch based on the photon-regulated interaction of WC-1 and VVD from Neurospora crassa.
we implemented FUN-LOV, an optogenetic switch based on the photon-regulated interaction of WC-1 and VVD, two LOV (light-oxygen-voltage) blue-light photoreceptors from the fungus Neurospora crassa
FUN-LOV is an optogenetic switch based on the photon-regulated interaction of WC-1 and VVD from Neurospora crassa.
we implemented FUN-LOV, an optogenetic switch based on the photon-regulated interaction of WC-1 and VVD, two LOV (light-oxygen-voltage) blue-light photoreceptors from the fungus Neurospora crassa
FUN-LOV is an optogenetic switch based on the photon-regulated interaction of WC-1 and VVD from Neurospora crassa.
we implemented FUN-LOV, an optogenetic switch based on the photon-regulated interaction of WC-1 and VVD, two LOV (light-oxygen-voltage) blue-light photoreceptors from the fungus Neurospora crassa
FUN-LOV is an optogenetic switch based on the photon-regulated interaction of WC-1 and VVD from Neurospora crassa.
we implemented FUN-LOV, an optogenetic switch based on the photon-regulated interaction of WC-1 and VVD, two LOV (light-oxygen-voltage) blue-light photoreceptors from the fungus Neurospora crassa
FUN-LOV is an optogenetic switch based on the photon-regulated interaction of WC-1 and VVD from Neurospora crassa.
we implemented FUN-LOV, an optogenetic switch based on the photon-regulated interaction of WC-1 and VVD, two LOV (light-oxygen-voltage) blue-light photoreceptors from the fungus Neurospora crassa
FUN-LOV is an optogenetic switch based on the photon-regulated interaction of the fungal blue-light photoreceptors WC-1 and VVD from Neurospora crassa.
we implemented FUN-LOV; an optogenetic switch based on the photon-regulated interaction of WC-1 and VVD, two LOV (Light Oxygen Voltage) blue-light photoreceptors from the fungus Neurospora crassa
FUN-LOV is an optogenetic switch based on the photon-regulated interaction of the fungal blue-light photoreceptors WC-1 and VVD from Neurospora crassa.
we implemented FUN-LOV; an optogenetic switch based on the photon-regulated interaction of WC-1 and VVD, two LOV (Light Oxygen Voltage) blue-light photoreceptors from the fungus Neurospora crassa
FUN-LOV is an optogenetic switch based on the photon-regulated interaction of the fungal blue-light photoreceptors WC-1 and VVD from Neurospora crassa.
we implemented FUN-LOV; an optogenetic switch based on the photon-regulated interaction of WC-1 and VVD, two LOV (Light Oxygen Voltage) blue-light photoreceptors from the fungus Neurospora crassa
FUN-LOV is an optogenetic switch based on the photon-regulated interaction of the fungal blue-light photoreceptors WC-1 and VVD from Neurospora crassa.
we implemented FUN-LOV; an optogenetic switch based on the photon-regulated interaction of WC-1 and VVD, two LOV (Light Oxygen Voltage) blue-light photoreceptors from the fungus Neurospora crassa
FUN-LOV is an optogenetic switch based on the photon-regulated interaction of the fungal blue-light photoreceptors WC-1 and VVD from Neurospora crassa.
we implemented FUN-LOV; an optogenetic switch based on the photon-regulated interaction of WC-1 and VVD, two LOV (Light Oxygen Voltage) blue-light photoreceptors from the fungus Neurospora crassa
FUN-LOV is an optogenetic switch based on the photon-regulated interaction of the fungal blue-light photoreceptors WC-1 and VVD from Neurospora crassa.
we implemented FUN-LOV; an optogenetic switch based on the photon-regulated interaction of WC-1 and VVD, two LOV (Light Oxygen Voltage) blue-light photoreceptors from the fungus Neurospora crassa
FUN-LOV is an optogenetic switch based on the photon-regulated interaction of the fungal blue-light photoreceptors WC-1 and VVD from Neurospora crassa.
we implemented FUN-LOV; an optogenetic switch based on the photon-regulated interaction of WC-1 and VVD, two LOV (Light Oxygen Voltage) blue-light photoreceptors from the fungus Neurospora crassa
In yeast, FUN-LOV enables light-controlled gene expression with over 1,300-fold dynamic range measured by a luciferase reporter.
When tested in yeast, FUN-LOV yields light-controlled gene expression with exquisite temporal resolution and a broad dynamic range of over 1,300-fold, as measured by a luciferase reporter.
dynamic range 1300 fold
In yeast, FUN-LOV enables light-controlled gene expression with over 1,300-fold dynamic range measured by a luciferase reporter.
When tested in yeast, FUN-LOV yields light-controlled gene expression with exquisite temporal resolution and a broad dynamic range of over 1,300-fold, as measured by a luciferase reporter.
dynamic range 1300 fold
In yeast, FUN-LOV enables light-controlled gene expression with over 1,300-fold dynamic range measured by a luciferase reporter.
When tested in yeast, FUN-LOV yields light-controlled gene expression with exquisite temporal resolution and a broad dynamic range of over 1,300-fold, as measured by a luciferase reporter.
dynamic range 1300 fold
In yeast, FUN-LOV enables light-controlled gene expression with over 1,300-fold dynamic range measured by a luciferase reporter.
When tested in yeast, FUN-LOV yields light-controlled gene expression with exquisite temporal resolution and a broad dynamic range of over 1,300-fold, as measured by a luciferase reporter.
dynamic range 1300 fold
In yeast, FUN-LOV enables light-controlled gene expression with over 1,300-fold dynamic range measured by a luciferase reporter.
When tested in yeast, FUN-LOV yields light-controlled gene expression with exquisite temporal resolution and a broad dynamic range of over 1,300-fold, as measured by a luciferase reporter.
dynamic range 1300 fold
In yeast, FUN-LOV enables light-controlled gene expression with over 1,300-fold dynamic range measured by a luciferase reporter.
When tested in yeast, FUN-LOV yields light-controlled gene expression with exquisite temporal resolution and a broad dynamic range of over 1,300-fold, as measured by a luciferase reporter.
dynamic range 1300 fold
In yeast, FUN-LOV enables light-controlled gene expression with over 1,300-fold dynamic range measured by a luciferase reporter.
When tested in yeast, FUN-LOV yields light-controlled gene expression with exquisite temporal resolution and a broad dynamic range of over 1,300-fold, as measured by a luciferase reporter.
dynamic range 1300 fold
In yeast, FUN-LOV enables light-controlled gene expression with over 1300-fold dynamic range measured by a luciferase reporter.
When tested in yeast, FUN-LOV yields light-controlled gene expression with exquisite temporal resolution, and a broad dynamic range of over 1300-fold, as measured by a luciferase reporter.
dynamic range 1300
In yeast, FUN-LOV enables light-controlled gene expression with over 1300-fold dynamic range measured by a luciferase reporter.
When tested in yeast, FUN-LOV yields light-controlled gene expression with exquisite temporal resolution, and a broad dynamic range of over 1300-fold, as measured by a luciferase reporter.
dynamic range 1300
In yeast, FUN-LOV enables light-controlled gene expression with over 1300-fold dynamic range measured by a luciferase reporter.
When tested in yeast, FUN-LOV yields light-controlled gene expression with exquisite temporal resolution, and a broad dynamic range of over 1300-fold, as measured by a luciferase reporter.
dynamic range 1300
In yeast, FUN-LOV enables light-controlled gene expression with over 1300-fold dynamic range measured by a luciferase reporter.
When tested in yeast, FUN-LOV yields light-controlled gene expression with exquisite temporal resolution, and a broad dynamic range of over 1300-fold, as measured by a luciferase reporter.
dynamic range 1300
In yeast, FUN-LOV enables light-controlled gene expression with over 1300-fold dynamic range measured by a luciferase reporter.
When tested in yeast, FUN-LOV yields light-controlled gene expression with exquisite temporal resolution, and a broad dynamic range of over 1300-fold, as measured by a luciferase reporter.
dynamic range 1300
In yeast, FUN-LOV enables light-controlled gene expression with over 1300-fold dynamic range measured by a luciferase reporter.
When tested in yeast, FUN-LOV yields light-controlled gene expression with exquisite temporal resolution, and a broad dynamic range of over 1300-fold, as measured by a luciferase reporter.
dynamic range 1300
In yeast, FUN-LOV enables light-controlled gene expression with over 1300-fold dynamic range measured by a luciferase reporter.
When tested in yeast, FUN-LOV yields light-controlled gene expression with exquisite temporal resolution, and a broad dynamic range of over 1300-fold, as measured by a luciferase reporter.
dynamic range 1300
FUN-LOV-controlled expression of FLO1 yielded flocculation in light.
Light-controlled expression of the flocculin-encoding gene FLO1, by the FUN-LOV switch, yielded flocculation in light (FIL)
FUN-LOV-controlled expression of FLO1 yielded flocculation in light.
Light-controlled expression of the flocculin-encoding gene FLO1, by the FUN-LOV switch, yielded flocculation in light (FIL)
FUN-LOV-controlled expression of FLO1 yielded flocculation in light.
Light-controlled expression of the flocculin-encoding gene FLO1, by the FUN-LOV switch, yielded flocculation in light (FIL)
FUN-LOV-controlled expression of FLO1 yielded flocculation in light.
Light-controlled expression of the flocculin-encoding gene FLO1, by the FUN-LOV switch, yielded flocculation in light (FIL)
FUN-LOV-controlled expression of FLO1 yielded flocculation in light.
Light-controlled expression of the flocculin-encoding gene FLO1, by the FUN-LOV switch, yielded flocculation in light (FIL)
FUN-LOV-controlled expression of FLO1 yielded flocculation in light.
Light-controlled expression of the flocculin-encoding gene FLO1, by the FUN-LOV switch, yielded flocculation in light (FIL)
FUN-LOV-controlled expression of FLO1 yielded flocculation in light.
Light-controlled expression of the flocculin-encoding gene FLO1, by the FUN-LOV switch, yielded flocculation in light (FIL)
FUN-LOV-controlled expression of TUP1 provided flocculation in darkness.
the light-controlled expression of the corepressor TUP1 provided flocculation in darkness (FID)
FUN-LOV-controlled expression of TUP1 provided flocculation in darkness.
the light-controlled expression of the corepressor TUP1 provided flocculation in darkness (FID)
FUN-LOV-controlled expression of TUP1 provided flocculation in darkness.
the light-controlled expression of the corepressor TUP1 provided flocculation in darkness (FID)
FUN-LOV-controlled expression of TUP1 provided flocculation in darkness.
the light-controlled expression of the corepressor TUP1 provided flocculation in darkness (FID)
FUN-LOV-controlled expression of TUP1 provided flocculation in darkness.
the light-controlled expression of the corepressor TUP1 provided flocculation in darkness (FID)
FUN-LOV-controlled expression of TUP1 provided flocculation in darkness.
the light-controlled expression of the corepressor TUP1 provided flocculation in darkness (FID)
FUN-LOV-controlled expression of TUP1 provided flocculation in darkness.
the light-controlled expression of the corepressor TUP1 provided flocculation in darkness (FID)
Approval Evidence
6 sources25 linked approval claimsfirst-pass slug fun-lov
In this work, we used an optogenetic system named FUN-LOV (FUNgal-Light Oxygen Voltage) to regulate the expression of ADH1 and GPD1 in a wine yeast strain using light.
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the FUN-LOV (FUNgal Light Oxygen and Voltage) optogenetic switch
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we report on the modular optimization of the fungal light-oxygen-voltage (FUN-LOV) system, an optogenetic switch based on photoreceptors from the fungus Neurospora crassa.
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We then revise an example of a previously described yeast optogenetic switch, named FUN-LOV, which allows precise and strong activation of the target gene.
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we implemented FUN-LOV, an optogenetic switch based on the photon-regulated interaction of WC-1 and VVD
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we implemented FUN-LOV; an optogenetic switch based on the photon-regulated interaction of WC-1 and VVD
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application scopesupports
Optogenetic tools can control yeast fermentation in a wine yeast strain and change the balance of metabolic products of interest in a light-dependent manner.
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expression controlsupports
Engineered strains showed light-controlled expression of GPD1 and ADH1.
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feasibilitysupports
FUN-LOV can be used to regulate ADH1 and GPD1 expression in a wine yeast strain using light.
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metabolic effectsupports
Optogenetic control of ADH1 showed an inverted phenotype in which glycerol production increased under constant darkness conditions.
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metabolic effectsupports
Optogenetic control of GPD1 increased glycerol production under constant illumination without affecting ethanol production.
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functional capabilitysupports
FUN-LOV enables high levels of light-activated gene expression in Saccharomyces cerevisiae in a reversible and tunable fashion.
In the budding yeast Saccharomyces cerevisiae, the FUN-LOV (FUNgal Light Oxygen and Voltage) optogenetic switch enables high levels of light-activated gene expression in a reversible and tunable fashion.
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comparative performancesupports
Combining the Hap1p DNA-binding domain with either p65 or VP16 activation domains increased reporter expression relative to the original switch.
the combination of the Hap1p DBD with either p65 or VP16 activation domains also resulted in higher levels of reporter expression compared to the original switch
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comparative performancesupports
The HAP-LOV variant showed higher luciferase expression upon induction than FUN-LOV.
the variant carrying the Hap1p DBD, which we call "HAP-LOV", displayed higher levels of luciferase expression upon induction compared to FUN-LOV
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design parameter effectsupports
Using low-copy plasmids and strong promoters for expression of FUN-LOV and HAP-LOV components produced a stronger response in both systems.
when low-copy plasmids and strong promoters were used, a stronger response was achieved in both systems
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optimization reportsupports
This paper reports modular optimization of the FUN-LOV optogenetic switch in Saccharomyces cerevisiae.
In Saccharomyces cerevisiae... we report on the modular optimization of the fungal light-oxygen-voltage (FUN-LOV) system
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toolkit expansionsupports
The study describes a new set of blue-light optogenetic switches carrying different protein modules that expands the available suite of optogenetic tools in yeast.
we describe a new set of blue-light optogenetic switches carrying different protein modules, which expands the available suite of optogenetic tools in yeast
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variant generationsupports
New FUN-LOV switch variants were generated by replacing the Gal4 DNA-binding domain with nine different zinc cluster family yeast transcription factor DNA-binding domains.
We also describe new switch variants obtained by replacing the Gal4 DNA-binding domain (DBD) of FUN-LOV with nine different DBDs from yeast transcription factors of the zinc cluster family.
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functional capabilitysupports
Optogenetic systems implemented in budding yeast Saccharomyces cerevisiae allow orthogonal light control of gene expression, protein subcellular localization, reconstitution of protein activity, and protein sequestration by oligomerization.
the main optogenetic systems implemented in the budding yeast Saccharomyces cerevisiae, which allow orthogonal control (by light) of gene expression, protein subcellular localization, reconstitution of protein activity, and protein sequestration by oligomerization
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implementation statussupports
Optogenetics has been successfully implemented in yeast.
Optogenetics has been successfully implemented in yeast
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performance statementsupports
FUN-LOV allows precise and strong activation of the target gene.
FUN-LOV, which allows precise and strong activation of the target gene
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applicationsupports
FUN-LOV can control yeast flocculation through light-regulated expression programs, producing Flocculation in Light via FLO1 expression and Flocculation in Darkness via TUP1 expression.
Light-controlled expression of the flocculin encoding gene FLO1, by the FUN-LOV switch, yielded Flocculation in Light (FIL), whereas the light-controlled expression of the co-repressor TUP1 provided Flocculation in Darkness (FID).
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background leakagesupports
In yeast cells, FUN-LOV allowed tight regulation of gene expression with low background in darkness and potent control by light.
In yeast cells, FUN-LOV allowed tight regulation of gene expression, with low background in darkness and a highly dynamic and potent control by light.
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comparative performancesupports
In Saccharomyces cerevisiae, FUN-LOV-driven heterologous protein expression under light stimulation exceeded expression from a classic GAL4/galactose inducible system by 2.5-fold.
Western blot analysis confirmed strong induction upon light stimulation, surpassing by 2.5 times the levels achieved with a classic GAL4 /galactose chemical inducible system.
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comparative performancesupports
In Saccharomyces cerevisiae, FUN-LOV-driven heterologous protein expression under light stimulation surpassed a classic GAL4/galactose inducible system by 2.5-fold.
Western blot analysis confirmed strong induction upon light stimulation, surpassing by 2.5 times the levels achieved with a classic GAL4/galactose chemical-inducible system.
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implementationsupports
FUN-LOV is an optogenetic switch based on the photon-regulated interaction of WC-1 and VVD from Neurospora crassa.
we implemented FUN-LOV, an optogenetic switch based on the photon-regulated interaction of WC-1 and VVD, two LOV (light-oxygen-voltage) blue-light photoreceptors from the fungus Neurospora crassa
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Comparisons
Source-backed strengths
The available evidence describes FUN-LOV as allowing precise and strong activation of a target gene in yeast. It has also undergone modular optimization and has been applied beyond standard laboratory contexts to a wine yeast strain for light-controlled metabolic regulation.
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The results indicate that FUN-LOVSP-Nat and FUN-LOVSP-Hph, either episomally or genome integrated, reached higher levels of luciferase expression upon blue-light stimulation compared the original FUN-LOV system.
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the combination of the Hap1p DBD with either p65 or VP16 activation domains also resulted in higher levels of reporter expression compared to the original switch
Source:
the variant carrying the Hap1p DBD, which we call "HAP-LOV", displayed higher levels of luciferase expression upon induction compared to FUN-LOV
Source:
In Saccharomyces cerevisiae... we report on the modular optimization of the fungal light-oxygen-voltage (FUN-LOV) system
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