Source 1primary paper2022Frontiers in Bioengineering and Biotechnology
Toolkit/FUN-LOVSP-Hph
FUN-LOVSP-Hph
Also known as: FUN-LOVSP-Hph, FUN-LOVSP-Hph
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
FUN-LOVSP-Hph is a blue-light-responsive yeast optogenetic switch variant of FUN-LOVSP that includes a hygromycin resistance cassette for selection after genome integration. It drives light-induced reporter expression and was reported to outperform the original FUN-LOV system in BY4741 yeast while remaining functional in a wine yeast strain.
Usefulness & Problems
Why this is useful
This variant expands the applicability of the FUN-LOV optogenetic platform across different Saccharomyces cerevisiae strain backgrounds. Its hygromycin selection marker supports recovery of genome-integrated strains, and the system enables blue-light-controlled induction of reporter expression.
Source:
Altogether, the new FUN-LOV variants described here are functional in different yeast strains, expanding the biotechnological applications of this optogenetic tool.
Source:
In the budding yeast Saccharomyces cerevisiae, the FUN-LOV (FUNgal Light Oxygen and Voltage) optogenetic switch enables high levels of light-activated gene expression in a reversible and tunable fashion.
Problem solved
FUN-LOVSP-Hph addresses the need for a selectable, genome-integrable blue-light switch that functions beyond a single laboratory yeast background. It also addresses the need for improved induced expression relative to the original FUN-LOV system in BY4741 yeast.
Source:
Altogether, the new FUN-LOV variants described here are functional in different yeast strains, expanding the biotechnological applications of this optogenetic tool.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Techniques
Selection / EnrichmentTarget processes
selectionInput: Light
Implementation Constraints
The construct is a FUN-LOVSP variant carrying a hygromycin resistance cassette, indicating use for selection after genome integration. Reported validation was performed in yeast, including BY4741 and the 59A-EC1118 wine strain, and performance was assessed by luciferase expression under blue-light stimulation in episomal and genome-integrated formats.
The supplied evidence is limited to reporter-expression performance in yeast strains and does not provide quantitative fold changes, kinetic parameters, or mechanistic detail beyond blue-light responsiveness. No independent replication, non-yeast validation, or application to endogenous pathway control is described in the provided evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The new FUN-LOV variants are functional in different yeast strains and expand the biotechnological applications of the optogenetic tool.
Altogether, the new FUN-LOV variants described here are functional in different yeast strains, expanding the biotechnological applications of this optogenetic tool.
The new FUN-LOV variants are functional in different yeast strains and expand the biotechnological applications of the optogenetic tool.
Altogether, the new FUN-LOV variants described here are functional in different yeast strains, expanding the biotechnological applications of this optogenetic tool.
The new FUN-LOV variants are functional in different yeast strains and expand the biotechnological applications of the optogenetic tool.
Altogether, the new FUN-LOV variants described here are functional in different yeast strains, expanding the biotechnological applications of this optogenetic tool.
The new FUN-LOV variants are functional in different yeast strains and expand the biotechnological applications of the optogenetic tool.
Altogether, the new FUN-LOV variants described here are functional in different yeast strains, expanding the biotechnological applications of this optogenetic tool.
The new FUN-LOV variants are functional in different yeast strains and expand the biotechnological applications of the optogenetic tool.
Altogether, the new FUN-LOV variants described here are functional in different yeast strains, expanding the biotechnological applications of this optogenetic tool.
The new FUN-LOV variants are functional in different yeast strains and expand the biotechnological applications of the optogenetic tool.
Altogether, the new FUN-LOV variants described here are functional in different yeast strains, expanding the biotechnological applications of this optogenetic tool.
The new FUN-LOV variants are functional in different yeast strains and expand the biotechnological applications of the optogenetic tool.
Altogether, the new FUN-LOV variants described here are functional in different yeast strains, expanding the biotechnological applications of this optogenetic tool.
FUN-LOVSP-Nat and FUN-LOVSP-Hph reached higher luciferase expression upon blue-light stimulation than the original FUN-LOV system in BY4741 yeast, in both episomal and genome-integrated formats.
The results indicate that FUN-LOVSP-Nat and FUN-LOVSP-Hph, either episomally or genome integrated, reached higher levels of luciferase expression upon blue-light stimulation compared the original FUN-LOV system.
FUN-LOVSP-Nat and FUN-LOVSP-Hph reached higher luciferase expression upon blue-light stimulation than the original FUN-LOV system in BY4741 yeast, in both episomal and genome-integrated formats.
The results indicate that FUN-LOVSP-Nat and FUN-LOVSP-Hph, either episomally or genome integrated, reached higher levels of luciferase expression upon blue-light stimulation compared the original FUN-LOV system.
FUN-LOVSP-Nat and FUN-LOVSP-Hph reached higher luciferase expression upon blue-light stimulation than the original FUN-LOV system in BY4741 yeast, in both episomal and genome-integrated formats.
The results indicate that FUN-LOVSP-Nat and FUN-LOVSP-Hph, either episomally or genome integrated, reached higher levels of luciferase expression upon blue-light stimulation compared the original FUN-LOV system.
FUN-LOVSP-Nat and FUN-LOVSP-Hph reached higher luciferase expression upon blue-light stimulation than the original FUN-LOV system in BY4741 yeast, in both episomal and genome-integrated formats.
The results indicate that FUN-LOVSP-Nat and FUN-LOVSP-Hph, either episomally or genome integrated, reached higher levels of luciferase expression upon blue-light stimulation compared the original FUN-LOV system.
FUN-LOVSP-Nat and FUN-LOVSP-Hph reached higher luciferase expression upon blue-light stimulation than the original FUN-LOV system in BY4741 yeast, in both episomal and genome-integrated formats.
The results indicate that FUN-LOVSP-Nat and FUN-LOVSP-Hph, either episomally or genome integrated, reached higher levels of luciferase expression upon blue-light stimulation compared the original FUN-LOV system.
FUN-LOVSP-Nat and FUN-LOVSP-Hph reached higher luciferase expression upon blue-light stimulation than the original FUN-LOV system in BY4741 yeast, in both episomal and genome-integrated formats.
The results indicate that FUN-LOVSP-Nat and FUN-LOVSP-Hph, either episomally or genome integrated, reached higher levels of luciferase expression upon blue-light stimulation compared the original FUN-LOV system.
FUN-LOVSP-Nat and FUN-LOVSP-Hph reached higher luciferase expression upon blue-light stimulation than the original FUN-LOV system in BY4741 yeast, in both episomal and genome-integrated formats.
The results indicate that FUN-LOVSP-Nat and FUN-LOVSP-Hph, either episomally or genome integrated, reached higher levels of luciferase expression upon blue-light stimulation compared the original FUN-LOV system.
FUN-LOVSP-Hph was functional in the 59A-EC1118 wine yeast strain, with similar blue-light-induced reporter expression to the laboratory strain and lower luciferase background in darkness.
we demonstrated the functionality of FUN-LOVSP-Hph in the 59A-EC1118 wine yeast strain, showing similar levels of reporter gene induction under blue-light respect to the laboratory strain, and with lower luciferase expression background in darkness condition.
FUN-LOVSP-Hph was functional in the 59A-EC1118 wine yeast strain, with similar blue-light-induced reporter expression to the laboratory strain and lower luciferase background in darkness.
we demonstrated the functionality of FUN-LOVSP-Hph in the 59A-EC1118 wine yeast strain, showing similar levels of reporter gene induction under blue-light respect to the laboratory strain, and with lower luciferase expression background in darkness condition.
FUN-LOVSP-Hph was functional in the 59A-EC1118 wine yeast strain, with similar blue-light-induced reporter expression to the laboratory strain and lower luciferase background in darkness.
we demonstrated the functionality of FUN-LOVSP-Hph in the 59A-EC1118 wine yeast strain, showing similar levels of reporter gene induction under blue-light respect to the laboratory strain, and with lower luciferase expression background in darkness condition.
FUN-LOVSP-Hph was functional in the 59A-EC1118 wine yeast strain, with similar blue-light-induced reporter expression to the laboratory strain and lower luciferase background in darkness.
we demonstrated the functionality of FUN-LOVSP-Hph in the 59A-EC1118 wine yeast strain, showing similar levels of reporter gene induction under blue-light respect to the laboratory strain, and with lower luciferase expression background in darkness condition.
FUN-LOVSP-Hph was functional in the 59A-EC1118 wine yeast strain, with similar blue-light-induced reporter expression to the laboratory strain and lower luciferase background in darkness.
we demonstrated the functionality of FUN-LOVSP-Hph in the 59A-EC1118 wine yeast strain, showing similar levels of reporter gene induction under blue-light respect to the laboratory strain, and with lower luciferase expression background in darkness condition.
FUN-LOVSP-Hph was functional in the 59A-EC1118 wine yeast strain, with similar blue-light-induced reporter expression to the laboratory strain and lower luciferase background in darkness.
we demonstrated the functionality of FUN-LOVSP-Hph in the 59A-EC1118 wine yeast strain, showing similar levels of reporter gene induction under blue-light respect to the laboratory strain, and with lower luciferase expression background in darkness condition.
FUN-LOVSP-Hph was functional in the 59A-EC1118 wine yeast strain, with similar blue-light-induced reporter expression to the laboratory strain and lower luciferase background in darkness.
we demonstrated the functionality of FUN-LOVSP-Hph in the 59A-EC1118 wine yeast strain, showing similar levels of reporter gene induction under blue-light respect to the laboratory strain, and with lower luciferase expression background in darkness condition.
FUN-LOVSP is a single-plasmid variant of FUN-LOV generated by replacing promoter and terminator sequences and cloning the system into one plasmid.
Initially, we generated new variants of this system by replacing the promoter and terminator sequences and by cloning the system in a single plasmid (FUN-LOVSP).
FUN-LOVSP is a single-plasmid variant of FUN-LOV generated by replacing promoter and terminator sequences and cloning the system into one plasmid.
Initially, we generated new variants of this system by replacing the promoter and terminator sequences and by cloning the system in a single plasmid (FUN-LOVSP).
FUN-LOVSP is a single-plasmid variant of FUN-LOV generated by replacing promoter and terminator sequences and cloning the system into one plasmid.
Initially, we generated new variants of this system by replacing the promoter and terminator sequences and by cloning the system in a single plasmid (FUN-LOVSP).
FUN-LOVSP is a single-plasmid variant of FUN-LOV generated by replacing promoter and terminator sequences and cloning the system into one plasmid.
Initially, we generated new variants of this system by replacing the promoter and terminator sequences and by cloning the system in a single plasmid (FUN-LOVSP).
FUN-LOVSP is a single-plasmid variant of FUN-LOV generated by replacing promoter and terminator sequences and cloning the system into one plasmid.
Initially, we generated new variants of this system by replacing the promoter and terminator sequences and by cloning the system in a single plasmid (FUN-LOVSP).
FUN-LOVSP is a single-plasmid variant of FUN-LOV generated by replacing promoter and terminator sequences and cloning the system into one plasmid.
Initially, we generated new variants of this system by replacing the promoter and terminator sequences and by cloning the system in a single plasmid (FUN-LOVSP).
FUN-LOVSP is a single-plasmid variant of FUN-LOV generated by replacing promoter and terminator sequences and cloning the system into one plasmid.
Initially, we generated new variants of this system by replacing the promoter and terminator sequences and by cloning the system in a single plasmid (FUN-LOVSP).
FUN-LOVSP-Nat and FUN-LOVSP-Hph are FUN-LOVSP variants carrying nourseothricin or hygromycin resistance genes to allow selection after genome integration.
we included the nourseothricin (Nat) or hygromycin (Hph) antibiotic resistances genes in the new FUN-LOVSP plasmid, generating two new variants (FUN-LOVSP-Nat and FUN-LOVSP-Hph), to allow selection after genome integration.
FUN-LOVSP-Nat and FUN-LOVSP-Hph are FUN-LOVSP variants carrying nourseothricin or hygromycin resistance genes to allow selection after genome integration.
we included the nourseothricin (Nat) or hygromycin (Hph) antibiotic resistances genes in the new FUN-LOVSP plasmid, generating two new variants (FUN-LOVSP-Nat and FUN-LOVSP-Hph), to allow selection after genome integration.
FUN-LOVSP-Nat and FUN-LOVSP-Hph are FUN-LOVSP variants carrying nourseothricin or hygromycin resistance genes to allow selection after genome integration.
we included the nourseothricin (Nat) or hygromycin (Hph) antibiotic resistances genes in the new FUN-LOVSP plasmid, generating two new variants (FUN-LOVSP-Nat and FUN-LOVSP-Hph), to allow selection after genome integration.
FUN-LOVSP-Nat and FUN-LOVSP-Hph are FUN-LOVSP variants carrying nourseothricin or hygromycin resistance genes to allow selection after genome integration.
we included the nourseothricin (Nat) or hygromycin (Hph) antibiotic resistances genes in the new FUN-LOVSP plasmid, generating two new variants (FUN-LOVSP-Nat and FUN-LOVSP-Hph), to allow selection after genome integration.
FUN-LOVSP-Nat and FUN-LOVSP-Hph are FUN-LOVSP variants carrying nourseothricin or hygromycin resistance genes to allow selection after genome integration.
we included the nourseothricin (Nat) or hygromycin (Hph) antibiotic resistances genes in the new FUN-LOVSP plasmid, generating two new variants (FUN-LOVSP-Nat and FUN-LOVSP-Hph), to allow selection after genome integration.
FUN-LOVSP-Nat and FUN-LOVSP-Hph are FUN-LOVSP variants carrying nourseothricin or hygromycin resistance genes to allow selection after genome integration.
we included the nourseothricin (Nat) or hygromycin (Hph) antibiotic resistances genes in the new FUN-LOVSP plasmid, generating two new variants (FUN-LOVSP-Nat and FUN-LOVSP-Hph), to allow selection after genome integration.
FUN-LOVSP-Nat and FUN-LOVSP-Hph are FUN-LOVSP variants carrying nourseothricin or hygromycin resistance genes to allow selection after genome integration.
we included the nourseothricin (Nat) or hygromycin (Hph) antibiotic resistances genes in the new FUN-LOVSP plasmid, generating two new variants (FUN-LOVSP-Nat and FUN-LOVSP-Hph), to allow selection after genome integration.
FUN-LOV enables high levels of light-activated gene expression in Saccharomyces cerevisiae in a reversible and tunable fashion.
In the budding yeast Saccharomyces cerevisiae, the FUN-LOV (FUNgal Light Oxygen and Voltage) optogenetic switch enables high levels of light-activated gene expression in a reversible and tunable fashion.
FUN-LOV enables high levels of light-activated gene expression in Saccharomyces cerevisiae in a reversible and tunable fashion.
In the budding yeast Saccharomyces cerevisiae, the FUN-LOV (FUNgal Light Oxygen and Voltage) optogenetic switch enables high levels of light-activated gene expression in a reversible and tunable fashion.
FUN-LOV enables high levels of light-activated gene expression in Saccharomyces cerevisiae in a reversible and tunable fashion.
In the budding yeast Saccharomyces cerevisiae, the FUN-LOV (FUNgal Light Oxygen and Voltage) optogenetic switch enables high levels of light-activated gene expression in a reversible and tunable fashion.
FUN-LOV enables high levels of light-activated gene expression in Saccharomyces cerevisiae in a reversible and tunable fashion.
In the budding yeast Saccharomyces cerevisiae, the FUN-LOV (FUNgal Light Oxygen and Voltage) optogenetic switch enables high levels of light-activated gene expression in a reversible and tunable fashion.
FUN-LOV enables high levels of light-activated gene expression in Saccharomyces cerevisiae in a reversible and tunable fashion.
In the budding yeast Saccharomyces cerevisiae, the FUN-LOV (FUNgal Light Oxygen and Voltage) optogenetic switch enables high levels of light-activated gene expression in a reversible and tunable fashion.
FUN-LOV enables high levels of light-activated gene expression in Saccharomyces cerevisiae in a reversible and tunable fashion.
In the budding yeast Saccharomyces cerevisiae, the FUN-LOV (FUNgal Light Oxygen and Voltage) optogenetic switch enables high levels of light-activated gene expression in a reversible and tunable fashion.
FUN-LOV enables high levels of light-activated gene expression in Saccharomyces cerevisiae in a reversible and tunable fashion.
In the budding yeast Saccharomyces cerevisiae, the FUN-LOV (FUNgal Light Oxygen and Voltage) optogenetic switch enables high levels of light-activated gene expression in a reversible and tunable fashion.
Approval Evidence
1 source4 linked approval claimsfirst-pass slug fun-lovsp-hph
generating two new variants (FUN-LOVSP-Nat and FUN-LOVSP-Hph)
Source:
application scopesupports
The new FUN-LOV variants are functional in different yeast strains and expand the biotechnological applications of the optogenetic tool.
Altogether, the new FUN-LOV variants described here are functional in different yeast strains, expanding the biotechnological applications of this optogenetic tool.
Source:
comparative performancesupports
FUN-LOVSP-Nat and FUN-LOVSP-Hph reached higher luciferase expression upon blue-light stimulation than the original FUN-LOV system in BY4741 yeast, in both episomal and genome-integrated formats.
The results indicate that FUN-LOVSP-Nat and FUN-LOVSP-Hph, either episomally or genome integrated, reached higher levels of luciferase expression upon blue-light stimulation compared the original FUN-LOV system.
Source:
cross strain functionalitysupports
FUN-LOVSP-Hph was functional in the 59A-EC1118 wine yeast strain, with similar blue-light-induced reporter expression to the laboratory strain and lower luciferase background in darkness.
we demonstrated the functionality of FUN-LOVSP-Hph in the 59A-EC1118 wine yeast strain, showing similar levels of reporter gene induction under blue-light respect to the laboratory strain, and with lower luciferase expression background in darkness condition.
Source:
design modificationsupports
FUN-LOVSP-Nat and FUN-LOVSP-Hph are FUN-LOVSP variants carrying nourseothricin or hygromycin resistance genes to allow selection after genome integration.
we included the nourseothricin (Nat) or hygromycin (Hph) antibiotic resistances genes in the new FUN-LOVSP plasmid, generating two new variants (FUN-LOVSP-Nat and FUN-LOVSP-Hph), to allow selection after genome integration.
Source:
Comparisons
Source-backed strengths
In BY4741 yeast, FUN-LOVSP-Hph reached higher luciferase expression under blue-light stimulation than the original FUN-LOV system in both episomal and genome-integrated formats. It was also functional in the 59A-EC1118 wine yeast strain, showing similar blue-light-induced reporter expression to the laboratory strain and lower luciferase background in darkness.
Source:
The results indicate that FUN-LOVSP-Nat and FUN-LOVSP-Hph, either episomally or genome integrated, reached higher levels of luciferase expression upon blue-light stimulation compared the original FUN-LOV system.
Ranked Citations
- 1.