Source 1primary paper2022Frontiers in Bioengineering and Biotechnology
Toolkit/FUN-LOVSP-Nat
FUN-LOVSP-Nat
Also known as: FUN-LOVSP-Nat, FUN-LOVSP-Nat
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
FUN-LOVSP-Nat is a blue-light-responsive yeast optogenetic switch variant derived from the FUN-LOVSP system and carrying a nourseothricin resistance marker. In BY4741 yeast, it supported blue-light-induced luciferase expression that exceeded the original FUN-LOV system in both episomal and genome-integrated formats.
Usefulness & Problems
Why this is useful
This variant is useful for optogenetic gene-expression control in yeast while enabling selection through a nourseothricin resistance cassette after construct introduction or genome integration. The cited study indicates that new FUN-LOV variants broaden the biotechnological applicability of the platform across yeast backgrounds, although direct cross-strain data were not specifically reported for the Nat version.
Source:
Altogether, the new FUN-LOV variants described here are functional in different yeast strains, expanding the biotechnological applications of this optogenetic tool.
Source:
In the budding yeast Saccharomyces cerevisiae, the FUN-LOV (FUNgal Light Oxygen and Voltage) optogenetic switch enables high levels of light-activated gene expression in a reversible and tunable fashion.
Problem solved
FUN-LOVSP-Nat addresses the need to combine selectable-marker-based strain engineering with light-inducible transcriptional control in yeast. It also addresses the need for improved blue-light-driven reporter output relative to the original FUN-LOV system in BY4741.
Source:
Altogether, the new FUN-LOV variants described here are functional in different yeast strains, expanding the biotechnological applications of this optogenetic tool.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Techniques
Selection / EnrichmentTarget processes
selectionInput: Light
Implementation Constraints
The tool was reported in yeast and evaluated in the BY4741 laboratory strain using luciferase reporter expression under blue-light stimulation. It exists as a FUN-LOVSP-derived variant with a nourseothricin resistance marker and was tested in both episomal and genome-integrated formats.
The supplied evidence is limited to comparative luciferase reporter performance and marker identity, with no quantitative fold changes, kinetics, or photophysical parameters provided. Cross-strain functionality was demonstrated in the source claims for FUN-LOVSP-Hph, but not directly for FUN-LOVSP-Nat, so generalization to other yeast strains remains indirect.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The new FUN-LOV variants are functional in different yeast strains and expand the biotechnological applications of the optogenetic tool.
Altogether, the new FUN-LOV variants described here are functional in different yeast strains, expanding the biotechnological applications of this optogenetic tool.
The new FUN-LOV variants are functional in different yeast strains and expand the biotechnological applications of the optogenetic tool.
Altogether, the new FUN-LOV variants described here are functional in different yeast strains, expanding the biotechnological applications of this optogenetic tool.
The new FUN-LOV variants are functional in different yeast strains and expand the biotechnological applications of the optogenetic tool.
Altogether, the new FUN-LOV variants described here are functional in different yeast strains, expanding the biotechnological applications of this optogenetic tool.
The new FUN-LOV variants are functional in different yeast strains and expand the biotechnological applications of the optogenetic tool.
Altogether, the new FUN-LOV variants described here are functional in different yeast strains, expanding the biotechnological applications of this optogenetic tool.
The new FUN-LOV variants are functional in different yeast strains and expand the biotechnological applications of the optogenetic tool.
Altogether, the new FUN-LOV variants described here are functional in different yeast strains, expanding the biotechnological applications of this optogenetic tool.
The new FUN-LOV variants are functional in different yeast strains and expand the biotechnological applications of the optogenetic tool.
Altogether, the new FUN-LOV variants described here are functional in different yeast strains, expanding the biotechnological applications of this optogenetic tool.
The new FUN-LOV variants are functional in different yeast strains and expand the biotechnological applications of the optogenetic tool.
Altogether, the new FUN-LOV variants described here are functional in different yeast strains, expanding the biotechnological applications of this optogenetic tool.
FUN-LOVSP-Nat and FUN-LOVSP-Hph reached higher luciferase expression upon blue-light stimulation than the original FUN-LOV system in BY4741 yeast, in both episomal and genome-integrated formats.
The results indicate that FUN-LOVSP-Nat and FUN-LOVSP-Hph, either episomally or genome integrated, reached higher levels of luciferase expression upon blue-light stimulation compared the original FUN-LOV system.
FUN-LOVSP-Nat and FUN-LOVSP-Hph reached higher luciferase expression upon blue-light stimulation than the original FUN-LOV system in BY4741 yeast, in both episomal and genome-integrated formats.
The results indicate that FUN-LOVSP-Nat and FUN-LOVSP-Hph, either episomally or genome integrated, reached higher levels of luciferase expression upon blue-light stimulation compared the original FUN-LOV system.
FUN-LOVSP-Nat and FUN-LOVSP-Hph reached higher luciferase expression upon blue-light stimulation than the original FUN-LOV system in BY4741 yeast, in both episomal and genome-integrated formats.
The results indicate that FUN-LOVSP-Nat and FUN-LOVSP-Hph, either episomally or genome integrated, reached higher levels of luciferase expression upon blue-light stimulation compared the original FUN-LOV system.
FUN-LOVSP-Nat and FUN-LOVSP-Hph reached higher luciferase expression upon blue-light stimulation than the original FUN-LOV system in BY4741 yeast, in both episomal and genome-integrated formats.
The results indicate that FUN-LOVSP-Nat and FUN-LOVSP-Hph, either episomally or genome integrated, reached higher levels of luciferase expression upon blue-light stimulation compared the original FUN-LOV system.
FUN-LOVSP-Nat and FUN-LOVSP-Hph reached higher luciferase expression upon blue-light stimulation than the original FUN-LOV system in BY4741 yeast, in both episomal and genome-integrated formats.
The results indicate that FUN-LOVSP-Nat and FUN-LOVSP-Hph, either episomally or genome integrated, reached higher levels of luciferase expression upon blue-light stimulation compared the original FUN-LOV system.
FUN-LOVSP-Nat and FUN-LOVSP-Hph reached higher luciferase expression upon blue-light stimulation than the original FUN-LOV system in BY4741 yeast, in both episomal and genome-integrated formats.
The results indicate that FUN-LOVSP-Nat and FUN-LOVSP-Hph, either episomally or genome integrated, reached higher levels of luciferase expression upon blue-light stimulation compared the original FUN-LOV system.
FUN-LOVSP-Nat and FUN-LOVSP-Hph reached higher luciferase expression upon blue-light stimulation than the original FUN-LOV system in BY4741 yeast, in both episomal and genome-integrated formats.
The results indicate that FUN-LOVSP-Nat and FUN-LOVSP-Hph, either episomally or genome integrated, reached higher levels of luciferase expression upon blue-light stimulation compared the original FUN-LOV system.
FUN-LOVSP-Hph was functional in the 59A-EC1118 wine yeast strain, with similar blue-light-induced reporter expression to the laboratory strain and lower luciferase background in darkness.
we demonstrated the functionality of FUN-LOVSP-Hph in the 59A-EC1118 wine yeast strain, showing similar levels of reporter gene induction under blue-light respect to the laboratory strain, and with lower luciferase expression background in darkness condition.
FUN-LOVSP-Hph was functional in the 59A-EC1118 wine yeast strain, with similar blue-light-induced reporter expression to the laboratory strain and lower luciferase background in darkness.
we demonstrated the functionality of FUN-LOVSP-Hph in the 59A-EC1118 wine yeast strain, showing similar levels of reporter gene induction under blue-light respect to the laboratory strain, and with lower luciferase expression background in darkness condition.
FUN-LOVSP-Hph was functional in the 59A-EC1118 wine yeast strain, with similar blue-light-induced reporter expression to the laboratory strain and lower luciferase background in darkness.
we demonstrated the functionality of FUN-LOVSP-Hph in the 59A-EC1118 wine yeast strain, showing similar levels of reporter gene induction under blue-light respect to the laboratory strain, and with lower luciferase expression background in darkness condition.
FUN-LOVSP-Hph was functional in the 59A-EC1118 wine yeast strain, with similar blue-light-induced reporter expression to the laboratory strain and lower luciferase background in darkness.
we demonstrated the functionality of FUN-LOVSP-Hph in the 59A-EC1118 wine yeast strain, showing similar levels of reporter gene induction under blue-light respect to the laboratory strain, and with lower luciferase expression background in darkness condition.
FUN-LOVSP-Hph was functional in the 59A-EC1118 wine yeast strain, with similar blue-light-induced reporter expression to the laboratory strain and lower luciferase background in darkness.
we demonstrated the functionality of FUN-LOVSP-Hph in the 59A-EC1118 wine yeast strain, showing similar levels of reporter gene induction under blue-light respect to the laboratory strain, and with lower luciferase expression background in darkness condition.
FUN-LOVSP-Hph was functional in the 59A-EC1118 wine yeast strain, with similar blue-light-induced reporter expression to the laboratory strain and lower luciferase background in darkness.
we demonstrated the functionality of FUN-LOVSP-Hph in the 59A-EC1118 wine yeast strain, showing similar levels of reporter gene induction under blue-light respect to the laboratory strain, and with lower luciferase expression background in darkness condition.
FUN-LOVSP-Hph was functional in the 59A-EC1118 wine yeast strain, with similar blue-light-induced reporter expression to the laboratory strain and lower luciferase background in darkness.
we demonstrated the functionality of FUN-LOVSP-Hph in the 59A-EC1118 wine yeast strain, showing similar levels of reporter gene induction under blue-light respect to the laboratory strain, and with lower luciferase expression background in darkness condition.
FUN-LOVSP is a single-plasmid variant of FUN-LOV generated by replacing promoter and terminator sequences and cloning the system into one plasmid.
Initially, we generated new variants of this system by replacing the promoter and terminator sequences and by cloning the system in a single plasmid (FUN-LOVSP).
FUN-LOVSP is a single-plasmid variant of FUN-LOV generated by replacing promoter and terminator sequences and cloning the system into one plasmid.
Initially, we generated new variants of this system by replacing the promoter and terminator sequences and by cloning the system in a single plasmid (FUN-LOVSP).
FUN-LOVSP is a single-plasmid variant of FUN-LOV generated by replacing promoter and terminator sequences and cloning the system into one plasmid.
Initially, we generated new variants of this system by replacing the promoter and terminator sequences and by cloning the system in a single plasmid (FUN-LOVSP).
FUN-LOVSP is a single-plasmid variant of FUN-LOV generated by replacing promoter and terminator sequences and cloning the system into one plasmid.
Initially, we generated new variants of this system by replacing the promoter and terminator sequences and by cloning the system in a single plasmid (FUN-LOVSP).
FUN-LOVSP is a single-plasmid variant of FUN-LOV generated by replacing promoter and terminator sequences and cloning the system into one plasmid.
Initially, we generated new variants of this system by replacing the promoter and terminator sequences and by cloning the system in a single plasmid (FUN-LOVSP).
FUN-LOVSP is a single-plasmid variant of FUN-LOV generated by replacing promoter and terminator sequences and cloning the system into one plasmid.
Initially, we generated new variants of this system by replacing the promoter and terminator sequences and by cloning the system in a single plasmid (FUN-LOVSP).
FUN-LOVSP is a single-plasmid variant of FUN-LOV generated by replacing promoter and terminator sequences and cloning the system into one plasmid.
Initially, we generated new variants of this system by replacing the promoter and terminator sequences and by cloning the system in a single plasmid (FUN-LOVSP).
FUN-LOVSP-Nat and FUN-LOVSP-Hph are FUN-LOVSP variants carrying nourseothricin or hygromycin resistance genes to allow selection after genome integration.
we included the nourseothricin (Nat) or hygromycin (Hph) antibiotic resistances genes in the new FUN-LOVSP plasmid, generating two new variants (FUN-LOVSP-Nat and FUN-LOVSP-Hph), to allow selection after genome integration.
FUN-LOVSP-Nat and FUN-LOVSP-Hph are FUN-LOVSP variants carrying nourseothricin or hygromycin resistance genes to allow selection after genome integration.
we included the nourseothricin (Nat) or hygromycin (Hph) antibiotic resistances genes in the new FUN-LOVSP plasmid, generating two new variants (FUN-LOVSP-Nat and FUN-LOVSP-Hph), to allow selection after genome integration.
FUN-LOVSP-Nat and FUN-LOVSP-Hph are FUN-LOVSP variants carrying nourseothricin or hygromycin resistance genes to allow selection after genome integration.
we included the nourseothricin (Nat) or hygromycin (Hph) antibiotic resistances genes in the new FUN-LOVSP plasmid, generating two new variants (FUN-LOVSP-Nat and FUN-LOVSP-Hph), to allow selection after genome integration.
FUN-LOVSP-Nat and FUN-LOVSP-Hph are FUN-LOVSP variants carrying nourseothricin or hygromycin resistance genes to allow selection after genome integration.
we included the nourseothricin (Nat) or hygromycin (Hph) antibiotic resistances genes in the new FUN-LOVSP plasmid, generating two new variants (FUN-LOVSP-Nat and FUN-LOVSP-Hph), to allow selection after genome integration.
FUN-LOVSP-Nat and FUN-LOVSP-Hph are FUN-LOVSP variants carrying nourseothricin or hygromycin resistance genes to allow selection after genome integration.
we included the nourseothricin (Nat) or hygromycin (Hph) antibiotic resistances genes in the new FUN-LOVSP plasmid, generating two new variants (FUN-LOVSP-Nat and FUN-LOVSP-Hph), to allow selection after genome integration.
FUN-LOVSP-Nat and FUN-LOVSP-Hph are FUN-LOVSP variants carrying nourseothricin or hygromycin resistance genes to allow selection after genome integration.
we included the nourseothricin (Nat) or hygromycin (Hph) antibiotic resistances genes in the new FUN-LOVSP plasmid, generating two new variants (FUN-LOVSP-Nat and FUN-LOVSP-Hph), to allow selection after genome integration.
FUN-LOVSP-Nat and FUN-LOVSP-Hph are FUN-LOVSP variants carrying nourseothricin or hygromycin resistance genes to allow selection after genome integration.
we included the nourseothricin (Nat) or hygromycin (Hph) antibiotic resistances genes in the new FUN-LOVSP plasmid, generating two new variants (FUN-LOVSP-Nat and FUN-LOVSP-Hph), to allow selection after genome integration.
FUN-LOV enables high levels of light-activated gene expression in Saccharomyces cerevisiae in a reversible and tunable fashion.
In the budding yeast Saccharomyces cerevisiae, the FUN-LOV (FUNgal Light Oxygen and Voltage) optogenetic switch enables high levels of light-activated gene expression in a reversible and tunable fashion.
FUN-LOV enables high levels of light-activated gene expression in Saccharomyces cerevisiae in a reversible and tunable fashion.
In the budding yeast Saccharomyces cerevisiae, the FUN-LOV (FUNgal Light Oxygen and Voltage) optogenetic switch enables high levels of light-activated gene expression in a reversible and tunable fashion.
FUN-LOV enables high levels of light-activated gene expression in Saccharomyces cerevisiae in a reversible and tunable fashion.
In the budding yeast Saccharomyces cerevisiae, the FUN-LOV (FUNgal Light Oxygen and Voltage) optogenetic switch enables high levels of light-activated gene expression in a reversible and tunable fashion.
FUN-LOV enables high levels of light-activated gene expression in Saccharomyces cerevisiae in a reversible and tunable fashion.
In the budding yeast Saccharomyces cerevisiae, the FUN-LOV (FUNgal Light Oxygen and Voltage) optogenetic switch enables high levels of light-activated gene expression in a reversible and tunable fashion.
FUN-LOV enables high levels of light-activated gene expression in Saccharomyces cerevisiae in a reversible and tunable fashion.
In the budding yeast Saccharomyces cerevisiae, the FUN-LOV (FUNgal Light Oxygen and Voltage) optogenetic switch enables high levels of light-activated gene expression in a reversible and tunable fashion.
FUN-LOV enables high levels of light-activated gene expression in Saccharomyces cerevisiae in a reversible and tunable fashion.
In the budding yeast Saccharomyces cerevisiae, the FUN-LOV (FUNgal Light Oxygen and Voltage) optogenetic switch enables high levels of light-activated gene expression in a reversible and tunable fashion.
FUN-LOV enables high levels of light-activated gene expression in Saccharomyces cerevisiae in a reversible and tunable fashion.
In the budding yeast Saccharomyces cerevisiae, the FUN-LOV (FUNgal Light Oxygen and Voltage) optogenetic switch enables high levels of light-activated gene expression in a reversible and tunable fashion.
Approval Evidence
1 source3 linked approval claimsfirst-pass slug fun-lovsp-nat
generating two new variants (FUN-LOVSP-Nat and FUN-LOVSP-Hph)
Source:
application scopesupports
The new FUN-LOV variants are functional in different yeast strains and expand the biotechnological applications of the optogenetic tool.
Altogether, the new FUN-LOV variants described here are functional in different yeast strains, expanding the biotechnological applications of this optogenetic tool.
Source:
comparative performancesupports
FUN-LOVSP-Nat and FUN-LOVSP-Hph reached higher luciferase expression upon blue-light stimulation than the original FUN-LOV system in BY4741 yeast, in both episomal and genome-integrated formats.
The results indicate that FUN-LOVSP-Nat and FUN-LOVSP-Hph, either episomally or genome integrated, reached higher levels of luciferase expression upon blue-light stimulation compared the original FUN-LOV system.
Source:
design modificationsupports
FUN-LOVSP-Nat and FUN-LOVSP-Hph are FUN-LOVSP variants carrying nourseothricin or hygromycin resistance genes to allow selection after genome integration.
we included the nourseothricin (Nat) or hygromycin (Hph) antibiotic resistances genes in the new FUN-LOVSP plasmid, generating two new variants (FUN-LOVSP-Nat and FUN-LOVSP-Hph), to allow selection after genome integration.
Source:
Comparisons
Source-backed strengths
In BY4741 yeast, FUN-LOVSP-Nat reached higher luciferase expression under blue-light stimulation than the original FUN-LOV system. This improved performance was observed in both episomal and genome-integrated formats, supporting use in multiple construct-deployment strategies.
Source:
The results indicate that FUN-LOVSP-Nat and FUN-LOVSP-Hph, either episomally or genome integrated, reached higher levels of luciferase expression upon blue-light stimulation compared the original FUN-LOV system.
Ranked Citations
- 1.