Source 1primary paper2014Molecular Biology of the Cell
Toolkit/Gβγ-sequestering domain
Gβγ-sequestering domain
Taxonomy: Mechanism Branch / Component. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The Gβγ-sequestering domain is a protein domain used in an optogenetic configuration to inhibit G protein signaling at selected plasma membrane regions. In the cited study, light-triggered recruitment of this domain to the plasma membrane produced localized suppression of G protein signaling.
Usefulness & Problems
Why this is useful
This domain is useful for spatially restricting inhibition of G protein signaling rather than perturbing signaling globally. The available evidence supports its use for generating subcellularly localized signaling perturbations at the plasma membrane.
Problem solved
It addresses the problem of inhibiting G protein signaling with subcellular precision. Specifically, light-triggered membrane recruitment enables localized suppression of signaling in a chosen plasma membrane region.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Component: A low-level protein part used inside a larger architecture that realizes a mechanism.
Mechanisms
gβγ sequestrationlight-triggered subcellular recruitmentlocalized inhibition of g protein signalingTechniques
No technique tags yet.
Target processes
localizationsignalingInput: Light
Implementation Constraints
The available evidence indicates that the domain was used in an optogenetic recruitment setup in which light triggers its localization to a selected plasma membrane region. No further practical details are provided here regarding construct architecture, fusion partners, illumination wavelength, cofactors, or expression system.
The supplied evidence is limited to a single statement from one study and does not define the domain sequence, binding specificity, kinetics, reversibility, or magnitude of inhibition. It also does not establish performance across multiple cell types, organisms, or signaling contexts.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Light-triggered recruitment of a truncated RGS protein or a Gβγ-sequestering domain to a selected plasma membrane region causes localized inhibition of G protein signaling.
Light-triggered recruitment of a truncated regulator of G protein signaling (RGS) protein or a Gβγ-sequestering domain to a selected region on the plasma membrane results in localized inhibition of G protein signaling.
Light-triggered recruitment of a truncated RGS protein or a Gβγ-sequestering domain to a selected plasma membrane region causes localized inhibition of G protein signaling.
Light-triggered recruitment of a truncated regulator of G protein signaling (RGS) protein or a Gβγ-sequestering domain to a selected region on the plasma membrane results in localized inhibition of G protein signaling.
Light-triggered recruitment of a truncated RGS protein or a Gβγ-sequestering domain to a selected plasma membrane region causes localized inhibition of G protein signaling.
Light-triggered recruitment of a truncated regulator of G protein signaling (RGS) protein or a Gβγ-sequestering domain to a selected region on the plasma membrane results in localized inhibition of G protein signaling.
Light-triggered recruitment of a truncated RGS protein or a Gβγ-sequestering domain to a selected plasma membrane region causes localized inhibition of G protein signaling.
Light-triggered recruitment of a truncated regulator of G protein signaling (RGS) protein or a Gβγ-sequestering domain to a selected region on the plasma membrane results in localized inhibition of G protein signaling.
Light-triggered recruitment of a truncated RGS protein or a Gβγ-sequestering domain to a selected plasma membrane region causes localized inhibition of G protein signaling.
Light-triggered recruitment of a truncated regulator of G protein signaling (RGS) protein or a Gβγ-sequestering domain to a selected region on the plasma membrane results in localized inhibition of G protein signaling.
Light-triggered recruitment of a truncated RGS protein or a Gβγ-sequestering domain to a selected plasma membrane region causes localized inhibition of G protein signaling.
Light-triggered recruitment of a truncated regulator of G protein signaling (RGS) protein or a Gβγ-sequestering domain to a selected region on the plasma membrane results in localized inhibition of G protein signaling.
Light-triggered recruitment of a truncated RGS protein or a Gβγ-sequestering domain to a selected plasma membrane region causes localized inhibition of G protein signaling.
Light-triggered recruitment of a truncated regulator of G protein signaling (RGS) protein or a Gβγ-sequestering domain to a selected region on the plasma membrane results in localized inhibition of G protein signaling.
Approval Evidence
1 source1 linked approval claimfirst-pass slug g-sequestering-domain
Light-triggered recruitment of ... a Gβγ-sequestering domain to a selected region on the plasma membrane results in localized inhibition of G protein signaling.
Source:
mechanism of actionsupports
Light-triggered recruitment of a truncated RGS protein or a Gβγ-sequestering domain to a selected plasma membrane region causes localized inhibition of G protein signaling.
Light-triggered recruitment of a truncated regulator of G protein signaling (RGS) protein or a Gβγ-sequestering domain to a selected region on the plasma membrane results in localized inhibition of G protein signaling.
Source:
Comparisons
Source-backed strengths
The reported strength is spatially localized inhibition of G protein signaling following light-triggered recruitment to the plasma membrane. The evidence directly supports subcellular control of signaling inhibition, but does not provide quantitative performance metrics in the supplied material.
Ranked Citations
- 1.