Source 1primary paper2008Journal of Neurochemistry
Toolkit/Galpha(15i3)
Galpha(15i3)
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Galpha(15i3) is a G protein chimera reported in HEK293 cells as a pathway-linking component that couples the sweet taste receptor heterodimer TAS1R2/TAS1R3 to an InsP3-dependent Ca2+ release pathway. It is described together with Galpha(16gust44) in receptor signaling assays.
Usefulness & Problems
Why this is useful
This chimera is useful as an assay-enabling coupling element that redirects TAS1R2/TAS1R3 signaling into an InsP3-dependent Ca2+ readout in HEK293 cells. The available evidence supports utility for cellular sweet receptor signaling measurements, but does not provide broader benchmarking.
Source:
Stable and transient depletion of CIB1 by short-hairpin RNA increased the Ca2+ response of HEK293 cells to the InsP3-generating ligands ATP, UTP and carbachol
Problem solved
Galpha(15i3) addresses the problem of linking TAS1R2/TAS1R3 activation to a measurable intracellular Ca2+ release pathway in HEK293 cells. This enables receptor signaling assays when native coupling is not directly configured for the desired InsP3-dependent readout.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Techniques
No technique tags yet.
Target processes
No target processes tagged yet.
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: multi component delivery burdenoperating role: actuatorswitch architecture: multi componentswitch architecture: recruitment
The reported implementation context is HEK293 cells co-used with the sweet taste receptor heterodimer TAS1R2/TAS1R3. The assay output is InsP3-dependent Ca2+ release, but the supplied evidence does not specify construct design, expression strategy, or additional cofactors.
Evidence is limited to a single cited study and a single cell context, HEK293 cells. No quantitative performance data, sequence or domain architecture, specificity analysis, or independent replication are provided in the supplied evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Depletion of CIB1 by short-hairpin RNA increases the Ca2+ response of HEK293 cells to InsP3-generating ligands ATP, UTP, and carbachol.
Stable and transient depletion of CIB1 by short-hairpin RNA increased the Ca2+ response of HEK293 cells to the InsP3-generating ligands ATP, UTP and carbachol
Depletion of CIB1 by short-hairpin RNA increases the Ca2+ response of HEK293 cells to InsP3-generating ligands ATP, UTP, and carbachol.
Stable and transient depletion of CIB1 by short-hairpin RNA increased the Ca2+ response of HEK293 cells to the InsP3-generating ligands ATP, UTP and carbachol
Depletion of CIB1 by short-hairpin RNA increases the Ca2+ response of HEK293 cells to InsP3-generating ligands ATP, UTP, and carbachol.
Stable and transient depletion of CIB1 by short-hairpin RNA increased the Ca2+ response of HEK293 cells to the InsP3-generating ligands ATP, UTP and carbachol
Depletion of CIB1 by short-hairpin RNA increases the Ca2+ response of HEK293 cells to InsP3-generating ligands ATP, UTP, and carbachol.
Stable and transient depletion of CIB1 by short-hairpin RNA increased the Ca2+ response of HEK293 cells to the InsP3-generating ligands ATP, UTP and carbachol
Depletion of CIB1 by short-hairpin RNA increases the Ca2+ response of HEK293 cells to InsP3-generating ligands ATP, UTP, and carbachol.
Stable and transient depletion of CIB1 by short-hairpin RNA increased the Ca2+ response of HEK293 cells to the InsP3-generating ligands ATP, UTP and carbachol
Depletion of CIB1 by short-hairpin RNA increases the Ca2+ response of HEK293 cells to InsP3-generating ligands ATP, UTP, and carbachol.
Stable and transient depletion of CIB1 by short-hairpin RNA increased the Ca2+ response of HEK293 cells to the InsP3-generating ligands ATP, UTP and carbachol
Depletion of CIB1 by short-hairpin RNA increases the Ca2+ response of HEK293 cells to InsP3-generating ligands ATP, UTP, and carbachol.
Stable and transient depletion of CIB1 by short-hairpin RNA increased the Ca2+ response of HEK293 cells to the InsP3-generating ligands ATP, UTP and carbachol
Depletion of CIB1 by short-hairpin RNA increases the Ca2+ response of HEK293 cells to InsP3-generating ligands ATP, UTP, and carbachol.
Stable and transient depletion of CIB1 by short-hairpin RNA increased the Ca2+ response of HEK293 cells to the InsP3-generating ligands ATP, UTP and carbachol
Depletion of CIB1 by short-hairpin RNA increases the Ca2+ response of HEK293 cells to InsP3-generating ligands ATP, UTP, and carbachol.
Stable and transient depletion of CIB1 by short-hairpin RNA increased the Ca2+ response of HEK293 cells to the InsP3-generating ligands ATP, UTP and carbachol
Depletion of CIB1 by short-hairpin RNA increases the Ca2+ response of HEK293 cells to InsP3-generating ligands ATP, UTP, and carbachol.
Stable and transient depletion of CIB1 by short-hairpin RNA increased the Ca2+ response of HEK293 cells to the InsP3-generating ligands ATP, UTP and carbachol
In HEK293 cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an InsP3-dependent Ca2+ release pathway.
In heterologous human embryonic kidney 293 (HEK293) cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an inositol 1,4,5-trisphosphate (InsP3)-dependent Ca2+ release pathway
In HEK293 cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an InsP3-dependent Ca2+ release pathway.
In heterologous human embryonic kidney 293 (HEK293) cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an inositol 1,4,5-trisphosphate (InsP3)-dependent Ca2+ release pathway
In HEK293 cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an InsP3-dependent Ca2+ release pathway.
In heterologous human embryonic kidney 293 (HEK293) cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an inositol 1,4,5-trisphosphate (InsP3)-dependent Ca2+ release pathway
In HEK293 cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an InsP3-dependent Ca2+ release pathway.
In heterologous human embryonic kidney 293 (HEK293) cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an inositol 1,4,5-trisphosphate (InsP3)-dependent Ca2+ release pathway
In HEK293 cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an InsP3-dependent Ca2+ release pathway.
In heterologous human embryonic kidney 293 (HEK293) cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an inositol 1,4,5-trisphosphate (InsP3)-dependent Ca2+ release pathway
In HEK293 cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an InsP3-dependent Ca2+ release pathway.
In heterologous human embryonic kidney 293 (HEK293) cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an inositol 1,4,5-trisphosphate (InsP3)-dependent Ca2+ release pathway
In HEK293 cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an InsP3-dependent Ca2+ release pathway.
In heterologous human embryonic kidney 293 (HEK293) cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an inositol 1,4,5-trisphosphate (InsP3)-dependent Ca2+ release pathway
In HEK293 cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an InsP3-dependent Ca2+ release pathway.
In heterologous human embryonic kidney 293 (HEK293) cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an inositol 1,4,5-trisphosphate (InsP3)-dependent Ca2+ release pathway
In HEK293 cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an InsP3-dependent Ca2+ release pathway.
In heterologous human embryonic kidney 293 (HEK293) cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an inositol 1,4,5-trisphosphate (InsP3)-dependent Ca2+ release pathway
In HEK293 cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an InsP3-dependent Ca2+ release pathway.
In heterologous human embryonic kidney 293 (HEK293) cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an inositol 1,4,5-trisphosphate (InsP3)-dependent Ca2+ release pathway
In HEK293 cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an InsP3-dependent Ca2+ release pathway.
In heterologous human embryonic kidney 293 (HEK293) cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an inositol 1,4,5-trisphosphate (InsP3)-dependent Ca2+ release pathway
In HEK293 cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an InsP3-dependent Ca2+ release pathway.
In heterologous human embryonic kidney 293 (HEK293) cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an inositol 1,4,5-trisphosphate (InsP3)-dependent Ca2+ release pathway
In HEK293 cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an InsP3-dependent Ca2+ release pathway.
In heterologous human embryonic kidney 293 (HEK293) cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an inositol 1,4,5-trisphosphate (InsP3)-dependent Ca2+ release pathway
In HEK293 cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an InsP3-dependent Ca2+ release pathway.
In heterologous human embryonic kidney 293 (HEK293) cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an inositol 1,4,5-trisphosphate (InsP3)-dependent Ca2+ release pathway
In HEK293 cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an InsP3-dependent Ca2+ release pathway.
In heterologous human embryonic kidney 293 (HEK293) cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an inositol 1,4,5-trisphosphate (InsP3)-dependent Ca2+ release pathway
In HEK293 cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an InsP3-dependent Ca2+ release pathway.
In heterologous human embryonic kidney 293 (HEK293) cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an inositol 1,4,5-trisphosphate (InsP3)-dependent Ca2+ release pathway
In HEK293 cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an InsP3-dependent Ca2+ release pathway.
In heterologous human embryonic kidney 293 (HEK293) cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an inositol 1,4,5-trisphosphate (InsP3)-dependent Ca2+ release pathway
Approval Evidence
1 source1 linked approval claimfirst-pass slug galpha-15i3
the G protein chimeras Galpha(16gust44) and Galpha(15i3)
Source:
pathway couplingsupports
In HEK293 cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an InsP3-dependent Ca2+ release pathway.
In heterologous human embryonic kidney 293 (HEK293) cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an inositol 1,4,5-trisphosphate (InsP3)-dependent Ca2+ release pathway
Source:
Comparisons
Source-backed strengths
The cited study explicitly reports that Galpha(15i3) links TAS1R2/TAS1R3 to an InsP3-dependent Ca2+ release pathway in HEK293 cells. This provides direct evidence for functional pathway coupling in a mammalian cell assay context.
Compared with Cry2-Cib photodimerizing pair
Galpha(15i3) and Cry2-Cib photodimerizing pair address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: heterodimerization
Strengths here: looks easier to implement in practice.
Compared with optoPAK1
Galpha(15i3) and optoPAK1 address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: heterodimerization
Strengths here: looks easier to implement in practice.
Compared with Opto-RhoGEFs
Galpha(15i3) and Opto-RhoGEFs address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: heterodimerization
Strengths here: looks easier to implement in practice.
Ranked Citations
- 1.