Source 1primary paper2008Journal of Neurochemistry
Toolkit/Galpha(16gust44)
Galpha(16gust44)
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Galpha(16gust44) is a G protein chimera used in HEK293 cells as part of a multi-component signaling switch. It couples the sweet taste receptor heterodimer TAS1R2/TAS1R3 to an InsP3-dependent intracellular Ca2+ release pathway.
Usefulness & Problems
Why this is useful
This chimera is useful for redirecting output from the sweet taste receptor dimer TAS1R2/TAS1R3 into a Ca2+-based readout in HEK293 cells. The available evidence supports its use as a pathway-coupling component in cell-based signaling assays, but does not provide broader performance benchmarking.
Source:
Stable and transient depletion of CIB1 by short-hairpin RNA increased the Ca2+ response of HEK293 cells to the InsP3-generating ligands ATP, UTP and carbachol
Problem solved
It addresses the problem of linking TAS1R2/TAS1R3 receptor activation to an InsP3-dependent Ca2+ release pathway in a heterologous HEK293 cell system. This enables receptor signaling to be monitored through intracellular calcium responses rather than relying on the receptor's native coupling context.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Techniques
No technique tags yet.
Target processes
No target processes tagged yet.
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: multi component delivery burdenoperating role: actuatorswitch architecture: multi componentswitch architecture: recruitment
The reported implementation context is HEK293 cells co-opting the sweet taste receptor heterodimer TAS1R2/TAS1R3 into an InsP3-dependent Ca2+ release pathway using the Galpha(16gust44) chimera. No additional practical details such as sequence design, expression strategy, stoichiometry, or delivery method are provided in the supplied evidence.
Evidence is limited to a single cited study and a narrow use case in HEK293 cells with TAS1R2/TAS1R3. The available material does not report quantitative performance, dynamic range, specificity relative to other GPCRs, construct architecture, or independent replication.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Depletion of CIB1 by short-hairpin RNA increases the Ca2+ response of HEK293 cells to InsP3-generating ligands ATP, UTP, and carbachol.
Stable and transient depletion of CIB1 by short-hairpin RNA increased the Ca2+ response of HEK293 cells to the InsP3-generating ligands ATP, UTP and carbachol
Depletion of CIB1 by short-hairpin RNA increases the Ca2+ response of HEK293 cells to InsP3-generating ligands ATP, UTP, and carbachol.
Stable and transient depletion of CIB1 by short-hairpin RNA increased the Ca2+ response of HEK293 cells to the InsP3-generating ligands ATP, UTP and carbachol
Depletion of CIB1 by short-hairpin RNA increases the Ca2+ response of HEK293 cells to InsP3-generating ligands ATP, UTP, and carbachol.
Stable and transient depletion of CIB1 by short-hairpin RNA increased the Ca2+ response of HEK293 cells to the InsP3-generating ligands ATP, UTP and carbachol
Depletion of CIB1 by short-hairpin RNA increases the Ca2+ response of HEK293 cells to InsP3-generating ligands ATP, UTP, and carbachol.
Stable and transient depletion of CIB1 by short-hairpin RNA increased the Ca2+ response of HEK293 cells to the InsP3-generating ligands ATP, UTP and carbachol
Depletion of CIB1 by short-hairpin RNA increases the Ca2+ response of HEK293 cells to InsP3-generating ligands ATP, UTP, and carbachol.
Stable and transient depletion of CIB1 by short-hairpin RNA increased the Ca2+ response of HEK293 cells to the InsP3-generating ligands ATP, UTP and carbachol
Depletion of CIB1 by short-hairpin RNA increases the Ca2+ response of HEK293 cells to InsP3-generating ligands ATP, UTP, and carbachol.
Stable and transient depletion of CIB1 by short-hairpin RNA increased the Ca2+ response of HEK293 cells to the InsP3-generating ligands ATP, UTP and carbachol
Depletion of CIB1 by short-hairpin RNA increases the Ca2+ response of HEK293 cells to InsP3-generating ligands ATP, UTP, and carbachol.
Stable and transient depletion of CIB1 by short-hairpin RNA increased the Ca2+ response of HEK293 cells to the InsP3-generating ligands ATP, UTP and carbachol
Depletion of CIB1 by short-hairpin RNA increases the Ca2+ response of HEK293 cells to InsP3-generating ligands ATP, UTP, and carbachol.
Stable and transient depletion of CIB1 by short-hairpin RNA increased the Ca2+ response of HEK293 cells to the InsP3-generating ligands ATP, UTP and carbachol
Depletion of CIB1 by short-hairpin RNA increases the Ca2+ response of HEK293 cells to InsP3-generating ligands ATP, UTP, and carbachol.
Stable and transient depletion of CIB1 by short-hairpin RNA increased the Ca2+ response of HEK293 cells to the InsP3-generating ligands ATP, UTP and carbachol
Depletion of CIB1 by short-hairpin RNA increases the Ca2+ response of HEK293 cells to InsP3-generating ligands ATP, UTP, and carbachol.
Stable and transient depletion of CIB1 by short-hairpin RNA increased the Ca2+ response of HEK293 cells to the InsP3-generating ligands ATP, UTP and carbachol
In HEK293 cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an InsP3-dependent Ca2+ release pathway.
In heterologous human embryonic kidney 293 (HEK293) cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an inositol 1,4,5-trisphosphate (InsP3)-dependent Ca2+ release pathway
In HEK293 cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an InsP3-dependent Ca2+ release pathway.
In heterologous human embryonic kidney 293 (HEK293) cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an inositol 1,4,5-trisphosphate (InsP3)-dependent Ca2+ release pathway
In HEK293 cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an InsP3-dependent Ca2+ release pathway.
In heterologous human embryonic kidney 293 (HEK293) cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an inositol 1,4,5-trisphosphate (InsP3)-dependent Ca2+ release pathway
In HEK293 cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an InsP3-dependent Ca2+ release pathway.
In heterologous human embryonic kidney 293 (HEK293) cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an inositol 1,4,5-trisphosphate (InsP3)-dependent Ca2+ release pathway
In HEK293 cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an InsP3-dependent Ca2+ release pathway.
In heterologous human embryonic kidney 293 (HEK293) cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an inositol 1,4,5-trisphosphate (InsP3)-dependent Ca2+ release pathway
In HEK293 cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an InsP3-dependent Ca2+ release pathway.
In heterologous human embryonic kidney 293 (HEK293) cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an inositol 1,4,5-trisphosphate (InsP3)-dependent Ca2+ release pathway
In HEK293 cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an InsP3-dependent Ca2+ release pathway.
In heterologous human embryonic kidney 293 (HEK293) cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an inositol 1,4,5-trisphosphate (InsP3)-dependent Ca2+ release pathway
In HEK293 cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an InsP3-dependent Ca2+ release pathway.
In heterologous human embryonic kidney 293 (HEK293) cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an inositol 1,4,5-trisphosphate (InsP3)-dependent Ca2+ release pathway
In HEK293 cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an InsP3-dependent Ca2+ release pathway.
In heterologous human embryonic kidney 293 (HEK293) cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an inositol 1,4,5-trisphosphate (InsP3)-dependent Ca2+ release pathway
In HEK293 cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an InsP3-dependent Ca2+ release pathway.
In heterologous human embryonic kidney 293 (HEK293) cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an inositol 1,4,5-trisphosphate (InsP3)-dependent Ca2+ release pathway
In HEK293 cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an InsP3-dependent Ca2+ release pathway.
In heterologous human embryonic kidney 293 (HEK293) cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an inositol 1,4,5-trisphosphate (InsP3)-dependent Ca2+ release pathway
In HEK293 cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an InsP3-dependent Ca2+ release pathway.
In heterologous human embryonic kidney 293 (HEK293) cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an inositol 1,4,5-trisphosphate (InsP3)-dependent Ca2+ release pathway
In HEK293 cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an InsP3-dependent Ca2+ release pathway.
In heterologous human embryonic kidney 293 (HEK293) cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an inositol 1,4,5-trisphosphate (InsP3)-dependent Ca2+ release pathway
In HEK293 cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an InsP3-dependent Ca2+ release pathway.
In heterologous human embryonic kidney 293 (HEK293) cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an inositol 1,4,5-trisphosphate (InsP3)-dependent Ca2+ release pathway
In HEK293 cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an InsP3-dependent Ca2+ release pathway.
In heterologous human embryonic kidney 293 (HEK293) cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an inositol 1,4,5-trisphosphate (InsP3)-dependent Ca2+ release pathway
In HEK293 cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an InsP3-dependent Ca2+ release pathway.
In heterologous human embryonic kidney 293 (HEK293) cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an inositol 1,4,5-trisphosphate (InsP3)-dependent Ca2+ release pathway
In HEK293 cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an InsP3-dependent Ca2+ release pathway.
In heterologous human embryonic kidney 293 (HEK293) cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an inositol 1,4,5-trisphosphate (InsP3)-dependent Ca2+ release pathway
Approval Evidence
1 source1 linked approval claimfirst-pass slug galpha-16gust44
the G protein chimeras Galpha(16gust44) and Galpha(15i3)
Source:
pathway couplingsupports
In HEK293 cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an InsP3-dependent Ca2+ release pathway.
In heterologous human embryonic kidney 293 (HEK293) cells, the G protein chimeras Galpha(16gust44) and Galpha(15i3) link the sweet taste receptor dimer TAS1R2/TAS1R3 to an inositol 1,4,5-trisphosphate (InsP3)-dependent Ca2+ release pathway
Source:
Comparisons
Source-backed strengths
The cited study explicitly identifies Galpha(16gust44) as a chimera that links TAS1R2/TAS1R3 to InsP3-dependent Ca2+ release in HEK293 cells. This provides direct evidence for functional pathway redirection in a defined mammalian expression system.
Compared with Cry2-Cib photodimerizing pair
Galpha(16gust44) and Cry2-Cib photodimerizing pair address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: heterodimerization
Strengths here: looks easier to implement in practice.
Compared with optoPAK1
Galpha(16gust44) and optoPAK1 address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: heterodimerization
Strengths here: looks easier to implement in practice.
Compared with Opto-RhoGEFs
Galpha(16gust44) and Opto-RhoGEFs address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: heterodimerization
Strengths here: looks easier to implement in practice.
Ranked Citations
- 1.