Source 1primary paper2010Experimental Physiology
Toolkit/genetic probes for membrane potential monitoring
genetic probes for membrane potential monitoring
Also known as: membrane potential probes, voltage sensors
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Genetic probes for membrane potential monitoring are a specific focus... voltage sensors are the only currently explored approach that can possibly provide an accurate read-out of neuronal excitation within its full dynamic range.
Usefulness & Problems
Why this is useful
These genetically encoded reporters are used to monitor membrane potential optically. The abstract frames them as a direct read-out of neuronal excitation.; membrane potential monitoring; accurate read-out of neuronal excitation
Source:
These genetically encoded reporters are used to monitor membrane potential optically. The abstract frames them as a direct read-out of neuronal excitation.
Source:
membrane potential monitoring
Source:
accurate read-out of neuronal excitation
Problem solved
They address the need for direct monitoring of neuronal excitation rather than relying only on indirect activity indices such as Ca2+ probes.; provides direct optical read-out of neuronal excitation
Source:
They address the need for direct monitoring of neuronal excitation rather than relying only on indirect activity indices such as Ca2+ probes.
Source:
provides direct optical read-out of neuronal excitation
Problem links
provides direct optical read-out of neuronal excitation
LiteratureThey address the need for direct monitoring of neuronal excitation rather than relying only on indirect activity indices such as Ca2+ probes.
Source:
They address the need for direct monitoring of neuronal excitation rather than relying only on indirect activity indices such as Ca2+ probes.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Techniques
No technique tags yet.
Target processes
No target processes tagged yet.
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor
They require targeted genetic expression and appropriate optical instrumentation. The source specifically notes that optics are important for successful implementation.; requires optics suitable for successful implementation
The abstract indicates that these probes remain technically challenging because useful signal is confined to a very small membrane-adjacent compartment and background fluorescence reduces dynamic range.; generation of membrane potential probes is described as perhaps the most challenging direction; signal arises from a very fine subplasmalemma compartment; other fluorescence appears as background and decreases dynamic range
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
DREADDs allow activation of desired intracellular signaling pathways in defined groups of cells by systemic application of a synthetic biologically inert drug without implanting optrodes.
Light-sensitive chimeric GPCRs enable optical control of intracellular signaling and can switch on pathways associated with Gαs, Gαq, and Gαi.
Voltage sensors are the only currently explored approach mentioned here that can possibly provide an accurate read-out of neuronal excitation within its full dynamic range.
DREADDs can serve as an alternative to optogenetics or be combined with it to control intracellular signaling in defined cell groups.
Generation of membrane potential probes is particularly challenging because useful signal comes from a very small subplasmalemmal compartment while other fluorescence acts as background and reduces dynamic range.
Approval Evidence
1 source2 linked approval claimsfirst-pass slug genetic-probes-for-membrane-potential-monitoring
Genetic probes for membrane potential monitoring are a specific focus... voltage sensors are the only currently explored approach that can possibly provide an accurate read-out of neuronal excitation within its full dynamic range.
Source:
capabilitysupports
Voltage sensors are the only currently explored approach mentioned here that can possibly provide an accurate read-out of neuronal excitation within its full dynamic range.
Source:
limitationsupports
Generation of membrane potential probes is particularly challenging because useful signal comes from a very small subplasmalemmal compartment while other fluorescence acts as background and reduces dynamic range.
Source:
Comparisons
Source-stated alternatives
The source contrasts voltage sensors with indirect indices of neuronal activity such as Ca2+ probes.
Source:
The source contrasts voltage sensors with indirect indices of neuronal activity such as Ca2+ probes.
Source-backed strengths
described as the only currently explored approach that can possibly provide an accurate read-out of neuronal excitation within its full dynamic range
Source:
described as the only currently explored approach that can possibly provide an accurate read-out of neuronal excitation within its full dynamic range
Ranked Citations
- 1.