Source 1primary paper2020UNC Libraries
Toolkit/genetically encodable, light activatable heterodimerizer system
genetically encodable, light activatable heterodimerizer system
Also known as: light activatable heterodimerizer system
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The genetically encodable, light activatable heterodimerizer system is an optogenetic multi-component switch that places a protein cargo within a genetically encoded, light-responsive heterodimerization framework. In the cited application, incorporation of cofilin enabled illumination-dependent remodeling of the F-actin network and consequent changes in cell motility.
Usefulness & Problems
Why this is useful
This system is useful for coupling light input to inducible protein association in living cells through a genetically encodable design. The cited use case indicates utility for optically controlling actin cytoskeletal organization and motility-related behavior.
Source:
We describe herein an actin-remodelling protein cofilin that, when placed within the framework of a genetically encodable, light activatable heterodimerizer system, induces dramatic changes in the F-actin network and consequent cell motility upon illumination.
Problem solved
It addresses the problem of controlling intracellular effector function with light using a genetically encoded heterodimerization strategy. In the reported example, it enabled light-triggered control over cofilin-associated F-actin network changes linked to cell motility.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Techniques
No technique tags yet.
Target processes
No target processes tagged yet.
Input: Light
Implementation Constraints
The system is described as genetically encodable and based on a light-activatable heterodimerizer framework, implying expression of multiple engineered components. Beyond the fact that cofilin was placed within this framework, the supplied evidence does not specify construct architecture, cofactors, delivery method, or expression context.
The provided evidence does not identify the specific photoreceptor pair, wavelengths, kinetics, reversibility, dynamic range, or host system used. Validation is limited here to a single described application involving cofilin, F-actin remodeling, and motility.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Cofilin placed within a genetically encodable light-activatable heterodimerizer system induces dramatic changes in the F-actin network and consequent cell motility upon illumination.
We describe herein an actin-remodelling protein cofilin that, when placed within the framework of a genetically encodable, light activatable heterodimerizer system, induces dramatic changes in the F-actin network and consequent cell motility upon illumination.
Cofilin placed within a genetically encodable light-activatable heterodimerizer system induces dramatic changes in the F-actin network and consequent cell motility upon illumination.
We describe herein an actin-remodelling protein cofilin that, when placed within the framework of a genetically encodable, light activatable heterodimerizer system, induces dramatic changes in the F-actin network and consequent cell motility upon illumination.
Cofilin placed within a genetically encodable light-activatable heterodimerizer system induces dramatic changes in the F-actin network and consequent cell motility upon illumination.
We describe herein an actin-remodelling protein cofilin that, when placed within the framework of a genetically encodable, light activatable heterodimerizer system, induces dramatic changes in the F-actin network and consequent cell motility upon illumination.
Cofilin placed within a genetically encodable light-activatable heterodimerizer system induces dramatic changes in the F-actin network and consequent cell motility upon illumination.
We describe herein an actin-remodelling protein cofilin that, when placed within the framework of a genetically encodable, light activatable heterodimerizer system, induces dramatic changes in the F-actin network and consequent cell motility upon illumination.
Cofilin placed within a genetically encodable light-activatable heterodimerizer system induces dramatic changes in the F-actin network and consequent cell motility upon illumination.
We describe herein an actin-remodelling protein cofilin that, when placed within the framework of a genetically encodable, light activatable heterodimerizer system, induces dramatic changes in the F-actin network and consequent cell motility upon illumination.
Cofilin placed within a genetically encodable light-activatable heterodimerizer system induces dramatic changes in the F-actin network and consequent cell motility upon illumination.
We describe herein an actin-remodelling protein cofilin that, when placed within the framework of a genetically encodable, light activatable heterodimerizer system, induces dramatic changes in the F-actin network and consequent cell motility upon illumination.
Cofilin placed within a genetically encodable light-activatable heterodimerizer system induces dramatic changes in the F-actin network and consequent cell motility upon illumination.
We describe herein an actin-remodelling protein cofilin that, when placed within the framework of a genetically encodable, light activatable heterodimerizer system, induces dramatic changes in the F-actin network and consequent cell motility upon illumination.
Approval Evidence
1 source1 linked approval claimfirst-pass slug genetically-encodable-light-activatable-heterodimerizer-system
placed within the framework of a genetically encodable, light activatable heterodimerizer system
Source:
functional effectsupports
Cofilin placed within a genetically encodable light-activatable heterodimerizer system induces dramatic changes in the F-actin network and consequent cell motility upon illumination.
We describe herein an actin-remodelling protein cofilin that, when placed within the framework of a genetically encodable, light activatable heterodimerizer system, induces dramatic changes in the F-actin network and consequent cell motility upon illumination.
Source:
Comparisons
Source-backed strengths
The available evidence supports that illumination can produce dramatic changes in the F-actin network and consequent cell motility when cofilin is deployed in this system. Its genetically encodable format also supports implementation as an intracellular optogenetic control module.
Ranked Citations
- 1.