Source 1review2016Sensors
Toolkit/genetically encoded FRET biosensor
genetically encoded FRET biosensor
Also known as: genetically encoded FRET biosensors
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Förster or fluorescence resonance energy transfer (FRET) technology and genetically encoded FRET biosensors provide a powerful tool for visualizing signaling molecules in live cells with high spatiotemporal resolution.
Usefulness & Problems
Why this is useful
Genetically encoded FRET biosensors are described as tools for visualizing signaling molecules in live cells. They use FRET readout and are framed as compatible with biological imaging contexts.; visualizing signaling molecules in live cells; live-cell biosensing with high spatiotemporal resolution
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Genetically encoded FRET biosensors are described as tools for visualizing signaling molecules in live cells. They use FRET readout and are framed as compatible with biological imaging contexts.
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visualizing signaling molecules in live cells
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live-cell biosensing with high spatiotemporal resolution
Problem solved
They solve the need for live-cell, genetically encodable signaling readouts with high spatial and temporal resolution.; provides genetically encodable live-cell-compatible biosensing
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They solve the need for live-cell, genetically encodable signaling readouts with high spatial and temporal resolution.
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provides genetically encodable live-cell-compatible biosensing
Problem links
provides genetically encodable live-cell-compatible biosensing
LiteratureThey solve the need for live-cell, genetically encodable signaling readouts with high spatial and temporal resolution.
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They solve the need for live-cell, genetically encodable signaling readouts with high spatial and temporal resolution.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Mechanisms
förster resonance energy transferTechniques
No technique tags yet.
Target processes
signalingImplementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor
The abstract states that fluorescent proteins are most commonly used as both donor and acceptor fluorophores, and that FRET changes must be measured with appropriate methods.; requires donor and acceptor fluorophores; commonly implemented with fluorescent proteins; requires methods to measure FRET changes
The abstract does not establish that these biosensors solve all measurement challenges, and it notes that important factors must be considered when using fluorescent proteins for FRET studies.; performance depends on choice of fluorescent protein FRET pair
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Fluorescent proteins are most commonly used as both donor and acceptor fluorophores in FRET biosensors because they are genetically encodable and live-cell compatible.
FRET technology and genetically encoded FRET biosensors are useful for visualizing signaling molecules in live cells with high spatiotemporal resolution.
Approval Evidence
1 source2 linked approval claimsfirst-pass slug genetically-encoded-fret-biosensor
Förster or fluorescence resonance energy transfer (FRET) technology and genetically encoded FRET biosensors provide a powerful tool for visualizing signaling molecules in live cells with high spatiotemporal resolution.
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component usage summarysupports
Fluorescent proteins are most commonly used as both donor and acceptor fluorophores in FRET biosensors because they are genetically encodable and live-cell compatible.
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utility summarysupports
FRET technology and genetically encoded FRET biosensors are useful for visualizing signaling molecules in live cells with high spatiotemporal resolution.
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Comparisons
Source-stated alternatives
The abstract does not name non-FP biosensor alternatives, but it emphasizes fluorescent proteins as the most common donor and acceptor choice.
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The abstract does not name non-FP biosensor alternatives, but it emphasizes fluorescent proteins as the most common donor and acceptor choice.
Source-backed strengths
genetically encodable; live-cell compatible; high spatiotemporal resolution
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genetically encodable
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live-cell compatible
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high spatiotemporal resolution
Compared with engineered GEF-Pak1 interaction
genetically encoded FRET biosensor and engineered GEF-Pak1 interaction address a similar problem space because they share signaling.
Shared frame: same top-level item type; shared target processes: signaling
Compared with kinase translocation reporters
genetically encoded FRET biosensor and kinase translocation reporters address a similar problem space because they share signaling.
Shared frame: same top-level item type; shared target processes: signaling
genetically encoded FRET biosensor and novel fluorescent biosensor for mitochondrial outer membrane rupture address a similar problem space because they share signaling.
Shared frame: same top-level item type; shared target processes: signaling
Ranked Citations
- 1.