Source 1primary paper2025MED
Toolkit/genetically encoded gas vesicles
genetically encoded gas vesicles
Also known as: gas vesicles
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
We reported volumetric imaging of tumor gene expression at the cubic centimeter scale using genetically encoded gas vesicles
Usefulness & Problems
Why this is useful
Genetically encoded gas vesicles are used here as acoustic reporters for volumetric imaging of tumor gene expression. The abstract presents them as the reporter system for the gene-expression application.; ultrasound imaging of tumor gene expression; nonlinear acoustic reporting
Source:
Genetically encoded gas vesicles are used here as acoustic reporters for volumetric imaging of tumor gene expression. The abstract presents them as the reporter system for the gene-expression application.
Source:
ultrasound imaging of tumor gene expression
Source:
nonlinear acoustic reporting
Problem solved
They enable noninvasive ultrasound-based readout of gene expression in tumors at cubic-centimeter scale. This addresses the need for reporter systems compatible with deep-tissue ultrasound imaging.; provides a genetically encoded acoustic reporter for volumetric tumor gene-expression imaging
Source:
They enable noninvasive ultrasound-based readout of gene expression in tumors at cubic-centimeter scale. This addresses the need for reporter systems compatible with deep-tissue ultrasound imaging.
Source:
provides a genetically encoded acoustic reporter for volumetric tumor gene-expression imaging
Problem links
provides a genetically encoded acoustic reporter for volumetric tumor gene-expression imaging
LiteratureThey enable noninvasive ultrasound-based readout of gene expression in tumors at cubic-centimeter scale. This addresses the need for reporter systems compatible with deep-tissue ultrasound imaging.
Source:
They enable noninvasive ultrasound-based readout of gene expression in tumors at cubic-centimeter scale. This addresses the need for reporter systems compatible with deep-tissue ultrasound imaging.
Published Workflows
Objective: Enable fast volumetric imaging of targeted biological functions in opaque organs by combining nondiffractive ultrasound beams, a cross-amplitude modulation sequence, and nonlinear acoustic reporters.
Why it works: The abstract states that the method combines nondiffractive ultrasound beams, a cross-amplitude modulation sequence, and nonlinear acoustic reporters to enable fast and volumetric imaging in tissues where optical light-sheet microscopy is limited by scattering and photobleaching.
nonlinear acoustic reportingultrasound-based volumetric imagingnondiffractive ultrasound beamscross-amplitude modulationlocalization microscopy
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Mechanisms
nonlinear acoustic reportingTechniques
No technique tags yet.
Target processes
No target processes tagged yet.
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor
They must be genetically encoded in the biological system being imaged and used with the ultrasound imaging method described in the paper. The abstract does not specify the exact construct or host implementation.; requires expression as a genetically encoded reporter; used with nonlinear sound-sheet microscopy
The abstract does not show that gas vesicles are the reporter for vascular localization microscopy, which instead uses microbubbles. It also does not provide details on expression burden, delivery, or generalizability.; abstract does not specify construct design, host system, or delivery details
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The study reported localization microscopy of cerebral capillary networks using intravascular microbubble contrast agents.
The study reported volumetric imaging of tumor gene expression at cubic-centimeter scale using genetically encoded gas vesicles.
imaging scale cubic centimeter scale
Nonlinear sound-sheet microscopy provides approximately 64-fold faster imaging speed, 35-fold larger imaged volume, and 4-fold higher classical imaging resolution than the state of the art in biomolecular ultrasound.
classical imaging resolution increase 4 foldimaged volume increase 35 foldimaging speed acceleration 64 fold
Nonlinear sound-sheet microscopy enables fast and volumetric imaging of targeted biological functions using nondiffractive ultrasound beams, cross-amplitude modulation, and nonlinear acoustic reporters.
Approval Evidence
1 source1 linked approval claimfirst-pass slug genetically-encoded-gas-vesicles
We reported volumetric imaging of tumor gene expression at the cubic centimeter scale using genetically encoded gas vesicles
Source:
applicationsupports
The study reported volumetric imaging of tumor gene expression at cubic-centimeter scale using genetically encoded gas vesicles.
Source:
Comparisons
Source-stated alternatives
The abstract contrasts this reporter application with intravascular microbubble contrast agents used for cerebral capillary localization microscopy.
Source:
The abstract contrasts this reporter application with intravascular microbubble contrast agents used for cerebral capillary localization microscopy.
Source-backed strengths
genetically encoded reporter format supports imaging of gene expression
Source:
genetically encoded reporter format supports imaging of gene expression
Compared with localization microscopy
The abstract contrasts this reporter application with intravascular microbubble contrast agents used for cerebral capillary localization microscopy.
Shared frame: source-stated alternative in extracted literature
Strengths here: genetically encoded reporter format supports imaging of gene expression.
Relative tradeoffs: abstract does not specify construct design, host system, or delivery details.
Source:
The abstract contrasts this reporter application with intravascular microbubble contrast agents used for cerebral capillary localization microscopy.
Compared with microscopy
The abstract contrasts this reporter application with intravascular microbubble contrast agents used for cerebral capillary localization microscopy.
Shared frame: source-stated alternative in extracted literature
Strengths here: genetically encoded reporter format supports imaging of gene expression.
Relative tradeoffs: abstract does not specify construct design, host system, or delivery details.
Source:
The abstract contrasts this reporter application with intravascular microbubble contrast agents used for cerebral capillary localization microscopy.
Ranked Citations
- 1.