Source 1review2022Physiological Reviews
Toolkit/genetically encoded photoswitches
genetically encoded photoswitches
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Genetically encoded photoswitches are opsin-free optogenetic components that can be modularly engineered into protein scaffolds or host cells to control biological processes with light. The cited review places these systems within optophysiology, where they are used to interrogate cellular physiology.
Usefulness & Problems
Why this is useful
These tools are useful because optogenetics is described as noninvasive, rapidly responsive, tunably reversible, and capable of superior spatiotemporal resolution. In the cited context, opsin-free genetically encoded photoswitches support light-based dissection of cellular physiology.
Source:
We also highlight exemplary applications of opsin-free optogenetics in dissecting cellular physiology (designated "optophysiology")
Source:
Optogenetics combines light and genetics to enable precise control of living cells, tissues, and organisms with tailored functions.
Problem solved
Genetically encoded photoswitches help solve the problem of perturbing and interrogating cellular physiology with high spatial and temporal precision using light. The review specifically frames this application area as optophysiology.
Source:
We also highlight exemplary applications of opsin-free optogenetics in dissecting cellular physiology (designated "optophysiology")
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
photoswitchingTechniques
Computational DesignTarget processes
No target processes tagged yet.
Input: Light
Implementation Constraints
The available evidence indicates that these photoswitches are genetically encoded and can be modularly engineered into protein scaffolds or host cells. No further practical details are provided on construct architecture, cofactors, delivery methods, or expression systems.
The provided evidence does not specify particular photoswitch families, chromophores, wavelengths, dynamic ranges, or target proteins. It also does not report independent benchmarking or application-specific performance data for any individual genetically encoded photoswitch.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Optogenetics is described as noninvasive, rapidly responsive, tunably reversible, and having superior spatiotemporal resolution.
Optogenetics has the advantages of noninvasiveness, rapid responsiveness, tunable reversibility, and superior spatiotemporal resolution.
Optogenetics is described as noninvasive, rapidly responsive, tunably reversible, and having superior spatiotemporal resolution.
Optogenetics has the advantages of noninvasiveness, rapid responsiveness, tunable reversibility, and superior spatiotemporal resolution.
Optogenetics is described as noninvasive, rapidly responsive, tunably reversible, and having superior spatiotemporal resolution.
Optogenetics has the advantages of noninvasiveness, rapid responsiveness, tunable reversibility, and superior spatiotemporal resolution.
Optogenetics is described as noninvasive, rapidly responsive, tunably reversible, and having superior spatiotemporal resolution.
Optogenetics has the advantages of noninvasiveness, rapid responsiveness, tunable reversibility, and superior spatiotemporal resolution.
Optogenetics is described as noninvasive, rapidly responsive, tunably reversible, and having superior spatiotemporal resolution.
Optogenetics has the advantages of noninvasiveness, rapid responsiveness, tunable reversibility, and superior spatiotemporal resolution.
Optogenetics is described as noninvasive, rapidly responsive, tunably reversible, and having superior spatiotemporal resolution.
Optogenetics has the advantages of noninvasiveness, rapid responsiveness, tunable reversibility, and superior spatiotemporal resolution.
Optogenetics is described as noninvasive, rapidly responsive, tunably reversible, and having superior spatiotemporal resolution.
Optogenetics has the advantages of noninvasiveness, rapid responsiveness, tunable reversibility, and superior spatiotemporal resolution.
Opsin-free optogenetics is applied to dissect cellular physiology, which the review designates as optophysiology.
We also highlight exemplary applications of opsin-free optogenetics in dissecting cellular physiology (designated "optophysiology")
Opsin-free optogenetics is applied to dissect cellular physiology, which the review designates as optophysiology.
We also highlight exemplary applications of opsin-free optogenetics in dissecting cellular physiology (designated "optophysiology")
Opsin-free optogenetics is applied to dissect cellular physiology, which the review designates as optophysiology.
We also highlight exemplary applications of opsin-free optogenetics in dissecting cellular physiology (designated "optophysiology")
Opsin-free optogenetics is applied to dissect cellular physiology, which the review designates as optophysiology.
We also highlight exemplary applications of opsin-free optogenetics in dissecting cellular physiology (designated "optophysiology")
Opsin-free optogenetics is applied to dissect cellular physiology, which the review designates as optophysiology.
We also highlight exemplary applications of opsin-free optogenetics in dissecting cellular physiology (designated "optophysiology")
Opsin-free optogenetics is applied to dissect cellular physiology, which the review designates as optophysiology.
We also highlight exemplary applications of opsin-free optogenetics in dissecting cellular physiology (designated "optophysiology")
Opsin-free optogenetics is applied to dissect cellular physiology, which the review designates as optophysiology.
We also highlight exemplary applications of opsin-free optogenetics in dissecting cellular physiology (designated "optophysiology")
Optogenetics combines light and genetics to enable precise control of living cells, tissues, and organisms with tailored functions.
Optogenetics combines light and genetics to enable precise control of living cells, tissues, and organisms with tailored functions.
Optogenetics combines light and genetics to enable precise control of living cells, tissues, and organisms with tailored functions.
Optogenetics combines light and genetics to enable precise control of living cells, tissues, and organisms with tailored functions.
Optogenetics combines light and genetics to enable precise control of living cells, tissues, and organisms with tailored functions.
Optogenetics combines light and genetics to enable precise control of living cells, tissues, and organisms with tailored functions.
Optogenetics combines light and genetics to enable precise control of living cells, tissues, and organisms with tailored functions.
Optogenetics combines light and genetics to enable precise control of living cells, tissues, and organisms with tailored functions.
Optogenetics combines light and genetics to enable precise control of living cells, tissues, and organisms with tailored functions.
Optogenetics combines light and genetics to enable precise control of living cells, tissues, and organisms with tailored functions.
Optogenetics combines light and genetics to enable precise control of living cells, tissues, and organisms with tailored functions.
Optogenetics combines light and genetics to enable precise control of living cells, tissues, and organisms with tailored functions.
Optogenetics combines light and genetics to enable precise control of living cells, tissues, and organisms with tailored functions.
Optogenetics combines light and genetics to enable precise control of living cells, tissues, and organisms with tailored functions.
Naturally occurring or engineered photoreceptors and photosensitive domains respond to light at varying wavelengths and have expanded optogenetic tool development.
a plethora of naturally occurring or engineered photoreceptors or photosensitive domains that respond to light at varying wavelengths has ushered in the next chapter of optogenetics
Genetically encoded photoswitches can be modularly engineered into protein scaffolds or host cells to control diverse biological processes and support behavioral control and disease intervention in vivo.
Through protein engineering and synthetic biology approaches, genetically encoded photoswitches can be modularly engineered into protein scaffolds or host cells to control a myriad of biological processes, as well as to enable behavioral control and disease intervention in vivo.
Genetically encoded photoswitches can be modularly engineered into protein scaffolds or host cells to control diverse biological processes and support behavioral control and disease intervention in vivo.
Through protein engineering and synthetic biology approaches, genetically encoded photoswitches can be modularly engineered into protein scaffolds or host cells to control a myriad of biological processes, as well as to enable behavioral control and disease intervention in vivo.
Genetically encoded photoswitches can be modularly engineered into protein scaffolds or host cells to control diverse biological processes and support behavioral control and disease intervention in vivo.
Through protein engineering and synthetic biology approaches, genetically encoded photoswitches can be modularly engineered into protein scaffolds or host cells to control a myriad of biological processes, as well as to enable behavioral control and disease intervention in vivo.
Genetically encoded photoswitches can be modularly engineered into protein scaffolds or host cells to control diverse biological processes and support behavioral control and disease intervention in vivo.
Through protein engineering and synthetic biology approaches, genetically encoded photoswitches can be modularly engineered into protein scaffolds or host cells to control a myriad of biological processes, as well as to enable behavioral control and disease intervention in vivo.
Genetically encoded photoswitches can be modularly engineered into protein scaffolds or host cells to control diverse biological processes and support behavioral control and disease intervention in vivo.
Through protein engineering and synthetic biology approaches, genetically encoded photoswitches can be modularly engineered into protein scaffolds or host cells to control a myriad of biological processes, as well as to enable behavioral control and disease intervention in vivo.
Genetically encoded photoswitches can be modularly engineered into protein scaffolds or host cells to control diverse biological processes and support behavioral control and disease intervention in vivo.
Through protein engineering and synthetic biology approaches, genetically encoded photoswitches can be modularly engineered into protein scaffolds or host cells to control a myriad of biological processes, as well as to enable behavioral control and disease intervention in vivo.
Genetically encoded photoswitches can be modularly engineered into protein scaffolds or host cells to control diverse biological processes and support behavioral control and disease intervention in vivo.
Through protein engineering and synthetic biology approaches, genetically encoded photoswitches can be modularly engineered into protein scaffolds or host cells to control a myriad of biological processes, as well as to enable behavioral control and disease intervention in vivo.
Approval Evidence
1 source4 linked approval claimsfirst-pass slug genetically-encoded-photoswitches
genetically encoded photoswitches can be modularly engineered into protein scaffolds or host cells
Source:
advantage summarysupports
Optogenetics is described as noninvasive, rapidly responsive, tunably reversible, and having superior spatiotemporal resolution.
Optogenetics has the advantages of noninvasiveness, rapid responsiveness, tunable reversibility, and superior spatiotemporal resolution.
Source:
application scopesupports
Opsin-free optogenetics is applied to dissect cellular physiology, which the review designates as optophysiology.
We also highlight exemplary applications of opsin-free optogenetics in dissecting cellular physiology (designated "optophysiology")
Source:
capability summarysupports
Optogenetics combines light and genetics to enable precise control of living cells, tissues, and organisms with tailored functions.
Optogenetics combines light and genetics to enable precise control of living cells, tissues, and organisms with tailored functions.
Source:
engineering modularitysupports
Genetically encoded photoswitches can be modularly engineered into protein scaffolds or host cells to control diverse biological processes and support behavioral control and disease intervention in vivo.
Through protein engineering and synthetic biology approaches, genetically encoded photoswitches can be modularly engineered into protein scaffolds or host cells to control a myriad of biological processes, as well as to enable behavioral control and disease intervention in vivo.
Source:
Comparisons
Source-backed strengths
The source attributes optogenetic approaches with noninvasiveness, rapid response, tunable reversibility, and superior spatiotemporal resolution. It also states that genetically encoded photoswitches can be modularly engineered into protein scaffolds or host cells, supporting flexible integration into biological systems.
Source:
Optogenetics has the advantages of noninvasiveness, rapid responsiveness, tunable reversibility, and superior spatiotemporal resolution.
Source:
Through protein engineering and synthetic biology approaches, genetically encoded photoswitches can be modularly engineered into protein scaffolds or host cells to control a myriad of biological processes, as well as to enable behavioral control and disease intervention in vivo.
Ranked Citations
- 1.