Source 1primary paper2018Comprehensive series in photochemistry and photobiology/Comprehensive series in photochemical & photobiological sciences
Toolkit/Genetically encoded PhyB–PIF light-inducible dimerization system
Genetically encoded PhyB–PIF light-inducible dimerization system
Also known as: genetically encoded PhyB LID system, PhyB–PIF LID system
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The genetically encoded PhyB–PIF light-inducible dimerization system is an optogenetic multi-component switch that uses a PhyB-based light-induced dimerization interaction to control signal transduction. The cited chapter describes a genetically encoded implementation enabled by efficient phycocyanobilin synthesis in cultured mammalian cells and reports applications in cultured cells and animals.
Usefulness & Problems
Why this is useful
This system is useful for manipulating biochemical networks and signal transduction with light, including longer-wavelength red and infrared light. The chapter frames it as an optogenetic approach for quantitative biology and notes use in both cultured cells and animals.
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The genetically encoded PhyB–PIF system is presented as a light-inducible dimerization system for controlling signal transduction. The abstract frames it as an optogenetic tool for manipulating biochemical networks with temporal and spatial precision.
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controlling signal transduction
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temporal manipulation of biochemical networks
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spatial manipulation of biochemical networks
Problem solved
It addresses the need for artificial control of biochemical networks to support quantitative understanding of biological systems through light-inducible control of signal transduction. It also solves the practical problem of making a PhyB-based light-induced dimerization system genetically encoded in cultured mammalian cells by enabling efficient phycocyanobilin synthesis.
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It addresses the need for artificial manipulation of biochemical networks to support quantitative understanding of biological systems. It also solves the practical issue of making a PhyB-based LID system genetically encoded in mammalian cells.
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enables genetically encoded light-induced control of signal transduction
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enables use of longer-wavelength light such as red and infrared light
Problem links
enables genetically encoded light-induced control of signal transduction
LiteratureIt addresses the need for artificial manipulation of biochemical networks to support quantitative understanding of biological systems. It also solves the practical issue of making a PhyB-based LID system genetically encoded in mammalian cells.
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It addresses the need for artificial manipulation of biochemical networks to support quantitative understanding of biological systems. It also solves the practical issue of making a PhyB-based LID system genetically encoded in mammalian cells.
enables use of longer-wavelength light such as red and infrared light
LiteratureIt addresses the need for artificial manipulation of biochemical networks to support quantitative understanding of biological systems. It also solves the practical issue of making a PhyB-based LID system genetically encoded in mammalian cells.
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It addresses the need for artificial manipulation of biochemical networks to support quantitative understanding of biological systems. It also solves the practical issue of making a PhyB-based LID system genetically encoded in mammalian cells.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Techniques
No technique tags yet.
Target processes
No target processes tagged yet.
Input: Light
Implementation Constraints
cofactor dependency: requires exogenous cofactorencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: multi component delivery burdenimplementation constraint: spectral hardware requirementoperating role: actuatorswitch architecture: multi componentswitch architecture: recruitment
Implementation requires the PhyB-based light-inducible dimerization components and the chromophore phycocyanobilin. The chapter specifically states that efficient phycocyanobilin synthesis in cultured mammalian cells permits the system to be genetically encoded, and operation uses light input including red and infrared wavelengths.
The supplied evidence does not provide quantitative performance metrics, kinetic parameters, dynamic range, or failure modes for the system. It also does not establish comparative superiority over other optogenetic or chemically induced dimerization systems.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The chapter discusses application of the genetically encoded PhyB light-induced dimerization system to cultured cells and animals.
This chapter discusses recent advances in the LID system, including a genetically encoded PhyB LID system, and its application to cultured cells and animals.
A method for efficient synthesis of phycocyanobilin in cultured mammalian cells permits a PhyB-based light-induced dimerization system to be genetically encoded.
Recently, the authors developed a method for the efficient synthesis of phycocyanobilin, a chromophore of phytochrome B (PhyB), in cultured mammalian cells. This technique permits the LID system to be made with PhyB genetically encoded
The genetically encoded PhyB-based light-induced dimerization system enables manipulation of signal transduction with longer-wavelength light such as red and infrared light.
This technique permits the LID system to be made with PhyB genetically encoded and manipulation of signal transduction with light of longer wavelength such as red and infrared light.
Approval Evidence
1 source3 linked approval claimsfirst-pass slug genetically-encoded-phyb-pif-light-inducible-dimerization-system
This chapter discusses recent advances in the LID system, including a genetically encoded PhyB LID system, and its application to cultured cells and animals.
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application scopesupports
The chapter discusses application of the genetically encoded PhyB light-induced dimerization system to cultured cells and animals.
This chapter discusses recent advances in the LID system, including a genetically encoded PhyB LID system, and its application to cultured cells and animals.
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method enables toolsupports
A method for efficient synthesis of phycocyanobilin in cultured mammalian cells permits a PhyB-based light-induced dimerization system to be genetically encoded.
Recently, the authors developed a method for the efficient synthesis of phycocyanobilin, a chromophore of phytochrome B (PhyB), in cultured mammalian cells. This technique permits the LID system to be made with PhyB genetically encoded
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spectral capabilitysupports
The genetically encoded PhyB-based light-induced dimerization system enables manipulation of signal transduction with longer-wavelength light such as red and infrared light.
This technique permits the LID system to be made with PhyB genetically encoded and manipulation of signal transduction with light of longer wavelength such as red and infrared light.
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Comparisons
Source-stated alternatives
The abstract contrasts light-induced dimerization with chemically induced dimerization systems. It also notes that other photoresponsive proteins from fungi, cyanobacteria, plants, and modified fluorescent proteins are used in LID systems.
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The abstract contrasts light-induced dimerization with chemically induced dimerization systems. It also notes that other photoresponsive proteins from fungi, cyanobacteria, plants, and modified fluorescent proteins are used in LID systems.
Source-backed strengths
A key strength is that the system is genetically encoded in cultured mammalian cells when efficient phycocyanobilin synthesis is provided. Another stated advantage is the ability to manipulate signal transduction with longer-wavelength light such as red and infrared light, and the chapter reports applications in cultured cells and animals.
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LID has strong advantages in terms of temporal and spatial manipulations
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permits manipulation with red and infrared light
Compared with iLID/SspB
The abstract contrasts light-induced dimerization with chemically induced dimerization systems. It also notes that other photoresponsive proteins from fungi, cyanobacteria, plants, and modified fluorescent proteins are used in LID systems.
Shared frame: source-stated alternative in extracted literature
Strengths here: LID has strong advantages in terms of temporal and spatial manipulations; permits manipulation with red and infrared light.
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The abstract contrasts light-induced dimerization with chemically induced dimerization systems. It also notes that other photoresponsive proteins from fungi, cyanobacteria, plants, and modified fluorescent proteins are used in LID systems.
Ranked Citations
- 1.FoundationalSource 1Comprehensive series in photochemistry and photobiology/Comprehensive series in photochemical & photobiological sciences2018Claim 1Claim 2Claim 3
Derived from 3 linked claims. Example evidence: This chapter discusses recent advances in the LID system, including a genetically encoded PhyB LID system, and its application to cultured cells and animals.