Source 1primary paper2021Journal of Biological Chemistry
Toolkit/genetically encoded red fluorescence intensity-based small GTPase biosensors
genetically encoded red fluorescence intensity-based small GTPase biosensors
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Genetically encoded red fluorescence intensity-based small GTPase biosensors are assay reagents used to characterize optogenetic regulators of small GTPase activity. In the cited 2021 Journal of Biological Chemistry study, they were used to assess and confirm the specificities of iLID-based optogenetic small GTPase control tools.
Usefulness & Problems
Why this is useful
These biosensors provide a genetically encoded fluorescence readout for evaluating whether optogenetic perturbation tools act on the intended small GTPase pathway. Their use is specifically supported for characterization of light-inducible small GTPase regulators in signaling studies.
Source:
we constructed optogenetic tools to control the activity of small GTPases (RhoA, Rac1, Cdc42, Ras, Rap, and Ral) using an improved light-inducible dimer system (iLID)
Problem solved
They address the need for a reporter assay that can verify the specificity of optogenetic small GTPase control systems. In the cited work, this was important for confirming the behavior of iLID-based tools designed to control small GTPase activity.
Problem links
Need conditional control of signaling activity
DerivedGenetically encoded red fluorescence intensity-based small GTPase biosensors are assay reagents used to characterize optogenetic small GTPase control tools. In the cited study, they were used to confirm the specificities of iLID-based optogenetic regulators of small GTPase activity.
Need precise spatiotemporal control with light input
DerivedGenetically encoded red fluorescence intensity-based small GTPase biosensors are assay reagents used to characterize optogenetic small GTPase control tools. In the cited study, they were used to confirm the specificities of iLID-based optogenetic regulators of small GTPase activity.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Techniques
Functional AssayTarget processes
signalingInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: sensor
The biosensors are genetically encoded and were used as fluorescence intensity-based reporters in conjunction with optogenetic small GTPase control tools built on the improved light-inducible dimer system iLID. The provided evidence does not specify construct architecture, expression system, chromophore requirements, or imaging settings.
The supplied evidence does not identify the exact biosensor design, target GTPases, dynamic range, kinetics, or spectral parameters beyond red fluorescence intensity-based reporting. Validation is only documented here in the context of a single study characterizing iLID-based optogenetic tools.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The optogenetic small GTPase control tools were characterized with red fluorescence intensity-based small GTPase biosensors and their specificities were confirmed.
We characterized these optogenetic tools with genetically encoded red fluorescence intensity-based small GTPase biosensors and confirmed these optogenetic tools' specificities.
The optogenetic small GTPase control tools were characterized with red fluorescence intensity-based small GTPase biosensors and their specificities were confirmed.
We characterized these optogenetic tools with genetically encoded red fluorescence intensity-based small GTPase biosensors and confirmed these optogenetic tools' specificities.
The optogenetic small GTPase control tools were characterized with red fluorescence intensity-based small GTPase biosensors and their specificities were confirmed.
We characterized these optogenetic tools with genetically encoded red fluorescence intensity-based small GTPase biosensors and confirmed these optogenetic tools' specificities.
The optogenetic small GTPase control tools were characterized with red fluorescence intensity-based small GTPase biosensors and their specificities were confirmed.
We characterized these optogenetic tools with genetically encoded red fluorescence intensity-based small GTPase biosensors and confirmed these optogenetic tools' specificities.
The optogenetic small GTPase control tools were characterized with red fluorescence intensity-based small GTPase biosensors and their specificities were confirmed.
We characterized these optogenetic tools with genetically encoded red fluorescence intensity-based small GTPase biosensors and confirmed these optogenetic tools' specificities.
The optogenetic small GTPase control tools were characterized with red fluorescence intensity-based small GTPase biosensors and their specificities were confirmed.
We characterized these optogenetic tools with genetically encoded red fluorescence intensity-based small GTPase biosensors and confirmed these optogenetic tools' specificities.
The optogenetic small GTPase control tools were characterized with red fluorescence intensity-based small GTPase biosensors and their specificities were confirmed.
We characterized these optogenetic tools with genetically encoded red fluorescence intensity-based small GTPase biosensors and confirmed these optogenetic tools' specificities.
The optogenetic small GTPase control tools were characterized with red fluorescence intensity-based small GTPase biosensors and their specificities were confirmed.
We characterized these optogenetic tools with genetically encoded red fluorescence intensity-based small GTPase biosensors and confirmed these optogenetic tools' specificities.
The optogenetic small GTPase control tools were characterized with red fluorescence intensity-based small GTPase biosensors and their specificities were confirmed.
We characterized these optogenetic tools with genetically encoded red fluorescence intensity-based small GTPase biosensors and confirmed these optogenetic tools' specificities.
The optogenetic small GTPase control tools were characterized with red fluorescence intensity-based small GTPase biosensors and their specificities were confirmed.
We characterized these optogenetic tools with genetically encoded red fluorescence intensity-based small GTPase biosensors and confirmed these optogenetic tools' specificities.
The optogenetic small GTPase control tools were characterized with red fluorescence intensity-based small GTPase biosensors and their specificities were confirmed.
We characterized these optogenetic tools with genetically encoded red fluorescence intensity-based small GTPase biosensors and confirmed these optogenetic tools' specificities.
The optogenetic small GTPase control tools were characterized with red fluorescence intensity-based small GTPase biosensors and their specificities were confirmed.
We characterized these optogenetic tools with genetically encoded red fluorescence intensity-based small GTPase biosensors and confirmed these optogenetic tools' specificities.
The optogenetic small GTPase control tools were characterized with red fluorescence intensity-based small GTPase biosensors and their specificities were confirmed.
We characterized these optogenetic tools with genetically encoded red fluorescence intensity-based small GTPase biosensors and confirmed these optogenetic tools' specificities.
The optogenetic small GTPase control tools were characterized with red fluorescence intensity-based small GTPase biosensors and their specificities were confirmed.
We characterized these optogenetic tools with genetically encoded red fluorescence intensity-based small GTPase biosensors and confirmed these optogenetic tools' specificities.
The optogenetic small GTPase control tools were characterized with red fluorescence intensity-based small GTPase biosensors and their specificities were confirmed.
We characterized these optogenetic tools with genetically encoded red fluorescence intensity-based small GTPase biosensors and confirmed these optogenetic tools' specificities.
The optogenetic small GTPase control tools were characterized with red fluorescence intensity-based small GTPase biosensors and their specificities were confirmed.
We characterized these optogenetic tools with genetically encoded red fluorescence intensity-based small GTPase biosensors and confirmed these optogenetic tools' specificities.
The optogenetic small GTPase control tools were characterized with red fluorescence intensity-based small GTPase biosensors and their specificities were confirmed.
We characterized these optogenetic tools with genetically encoded red fluorescence intensity-based small GTPase biosensors and confirmed these optogenetic tools' specificities.
The authors constructed optogenetic tools to control the activity of small GTPases using the improved light-inducible dimer system iLID.
we constructed optogenetic tools to control the activity of small GTPases (RhoA, Rac1, Cdc42, Ras, Rap, and Ral) using an improved light-inducible dimer system (iLID)
The authors constructed optogenetic tools to control the activity of small GTPases using the improved light-inducible dimer system iLID.
we constructed optogenetic tools to control the activity of small GTPases (RhoA, Rac1, Cdc42, Ras, Rap, and Ral) using an improved light-inducible dimer system (iLID)
The authors constructed optogenetic tools to control the activity of small GTPases using the improved light-inducible dimer system iLID.
we constructed optogenetic tools to control the activity of small GTPases (RhoA, Rac1, Cdc42, Ras, Rap, and Ral) using an improved light-inducible dimer system (iLID)
The authors constructed optogenetic tools to control the activity of small GTPases using the improved light-inducible dimer system iLID.
we constructed optogenetic tools to control the activity of small GTPases (RhoA, Rac1, Cdc42, Ras, Rap, and Ral) using an improved light-inducible dimer system (iLID)
The authors constructed optogenetic tools to control the activity of small GTPases using the improved light-inducible dimer system iLID.
we constructed optogenetic tools to control the activity of small GTPases (RhoA, Rac1, Cdc42, Ras, Rap, and Ral) using an improved light-inducible dimer system (iLID)
The authors constructed optogenetic tools to control the activity of small GTPases using the improved light-inducible dimer system iLID.
we constructed optogenetic tools to control the activity of small GTPases (RhoA, Rac1, Cdc42, Ras, Rap, and Ral) using an improved light-inducible dimer system (iLID)
The authors constructed optogenetic tools to control the activity of small GTPases using the improved light-inducible dimer system iLID.
we constructed optogenetic tools to control the activity of small GTPases (RhoA, Rac1, Cdc42, Ras, Rap, and Ral) using an improved light-inducible dimer system (iLID)
The authors constructed optogenetic tools to control the activity of small GTPases using the improved light-inducible dimer system iLID.
we constructed optogenetic tools to control the activity of small GTPases (RhoA, Rac1, Cdc42, Ras, Rap, and Ral) using an improved light-inducible dimer system (iLID)
The authors constructed optogenetic tools to control the activity of small GTPases using the improved light-inducible dimer system iLID.
we constructed optogenetic tools to control the activity of small GTPases (RhoA, Rac1, Cdc42, Ras, Rap, and Ral) using an improved light-inducible dimer system (iLID)
The authors constructed optogenetic tools to control the activity of small GTPases using the improved light-inducible dimer system iLID.
we constructed optogenetic tools to control the activity of small GTPases (RhoA, Rac1, Cdc42, Ras, Rap, and Ral) using an improved light-inducible dimer system (iLID)
Approval Evidence
1 source1 linked approval claimfirst-pass slug genetically-encoded-red-fluorescence-intensity-based-small-gtpase-biosensors
We characterized these optogenetic tools with genetically encoded red fluorescence intensity-based small GTPase biosensors
Source:
specificitysupports
The optogenetic small GTPase control tools were characterized with red fluorescence intensity-based small GTPase biosensors and their specificities were confirmed.
We characterized these optogenetic tools with genetically encoded red fluorescence intensity-based small GTPase biosensors and confirmed these optogenetic tools' specificities.
Source:
Comparisons
Source-backed strengths
The available evidence shows that these biosensors were sufficient to characterize optogenetic small GTPase tools and confirm their specificities in the reported study. They are genetically encoded and red fluorescence intensity-based, which establishes the modality of the assay, but no quantitative performance metrics are provided in the supplied evidence.
Compared with cDNA microarray
genetically encoded red fluorescence intensity-based small GTPase biosensors and cDNA microarray address a similar problem space because they share signaling.
Shared frame: same top-level item type; shared target processes: signaling; same primary input modality: light
Compared with IRAP-pHluorin translocation assay
genetically encoded red fluorescence intensity-based small GTPase biosensors and IRAP-pHluorin translocation assay address a similar problem space because they share signaling.
Shared frame: same top-level item type; shared target processes: signaling; same primary input modality: light
genetically encoded red fluorescence intensity-based small GTPase biosensors and light-induced Fourier transform infrared (FTIR) difference spectroscopy address a similar problem space because they share signaling.
Shared frame: same top-level item type; shared target processes: signaling; same primary input modality: light
Ranked Citations
- 1.