Source 1review2016Cold Spring Harbor Perspectives in Biology
Toolkit/genome engineering
genome engineering
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Genome engineering is described in the cited review as a complementary engineering method that, together with progress in mammalian gene circuit design, may support the creation of tailored engineered mammalian cells for cell-based applications. The supplied evidence does not identify a specific genome engineering platform, molecular effector, or implementation.
Usefulness & Problems
Why this is useful
The evidence positions genome engineering as an enabling method that can be combined with synthetic gene switches and complex gene circuits in mammalian cells. In this context, its utility is framed broadly around supporting engineered mammalian cell functions relevant to future cell-based therapies, but no direct performance data are provided.
Source:
Their involvement in complex gene circuits results in sophisticated circuit topologies that are reminiscent of electronics and that are capable of providing engineered cells with the ability to memorize events, oscillate protein production, and perform complex information-processing tasks.
Source:
Microencapsulated mammalian cells that are engineered with closed-loop gene networks can be implanted into mice to sense disease-related input signals and to process this information to produce a custom, fine-tuned therapeutic response that rebalances animal metabolism.
Problem solved
The cited text suggests that genome engineering helps address the broader challenge of building tailored engineered mammalian cells when used alongside advances in gene circuit design. The specific technical problem solved by any particular genome engineering system is not defined in the supplied evidence.
Source:
Microencapsulated mammalian cells that are engineered with closed-loop gene networks can be implanted into mice to sense disease-related input signals and to process this information to produce a custom, fine-tuned therapeutic response that rebalances animal metabolism.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete method used to build, optimize, or evolve an engineered system.
Mechanisms
No mechanism tags yet.
Techniques
Computational DesignTarget processes
No target processes tagged yet.
Implementation Constraints
No practical implementation details are given for genome engineering in the supplied material. There is no information on construct architecture, host cell types beyond mammalian cells, delivery method, cofactors, or genomic target design.
The evidence is highly nonspecific and does not name a genome engineering modality such as CRISPR-Cas, recombinases, nucleases, or integrases. No data are provided on editing efficiency, targeting scope, delivery, safety, off-target effects, or validation in therapeutic settings.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Synthetic gene switches are described as basic building blocks for constructing complex gene circuits in mammalian cells.
Synthetic gene switches are basic building blocks for the construction of complex gene circuits
Synthetic gene switches are described as basic building blocks for constructing complex gene circuits in mammalian cells.
Synthetic gene switches are basic building blocks for the construction of complex gene circuits
Synthetic gene switches are described as basic building blocks for constructing complex gene circuits in mammalian cells.
Synthetic gene switches are basic building blocks for the construction of complex gene circuits
Synthetic gene switches are described as basic building blocks for constructing complex gene circuits in mammalian cells.
Synthetic gene switches are basic building blocks for the construction of complex gene circuits
Synthetic gene switches are described as basic building blocks for constructing complex gene circuits in mammalian cells.
Synthetic gene switches are basic building blocks for the construction of complex gene circuits
Synthetic gene switches are described as basic building blocks for constructing complex gene circuits in mammalian cells.
Synthetic gene switches are basic building blocks for the construction of complex gene circuits
Synthetic gene switches are described as basic building blocks for constructing complex gene circuits in mammalian cells.
Synthetic gene switches are basic building blocks for the construction of complex gene circuits
Complex gene circuits containing gene switches are described as capable of enabling mammalian cells to memorize events, oscillate protein production, and perform complex information-processing tasks.
Their involvement in complex gene circuits results in sophisticated circuit topologies that are reminiscent of electronics and that are capable of providing engineered cells with the ability to memorize events, oscillate protein production, and perform complex information-processing tasks.
Complex gene circuits containing gene switches are described as capable of enabling mammalian cells to memorize events, oscillate protein production, and perform complex information-processing tasks.
Their involvement in complex gene circuits results in sophisticated circuit topologies that are reminiscent of electronics and that are capable of providing engineered cells with the ability to memorize events, oscillate protein production, and perform complex information-processing tasks.
Complex gene circuits containing gene switches are described as capable of enabling mammalian cells to memorize events, oscillate protein production, and perform complex information-processing tasks.
Their involvement in complex gene circuits results in sophisticated circuit topologies that are reminiscent of electronics and that are capable of providing engineered cells with the ability to memorize events, oscillate protein production, and perform complex information-processing tasks.
Complex gene circuits containing gene switches are described as capable of enabling mammalian cells to memorize events, oscillate protein production, and perform complex information-processing tasks.
Their involvement in complex gene circuits results in sophisticated circuit topologies that are reminiscent of electronics and that are capable of providing engineered cells with the ability to memorize events, oscillate protein production, and perform complex information-processing tasks.
Complex gene circuits containing gene switches are described as capable of enabling mammalian cells to memorize events, oscillate protein production, and perform complex information-processing tasks.
Their involvement in complex gene circuits results in sophisticated circuit topologies that are reminiscent of electronics and that are capable of providing engineered cells with the ability to memorize events, oscillate protein production, and perform complex information-processing tasks.
Complex gene circuits containing gene switches are described as capable of enabling mammalian cells to memorize events, oscillate protein production, and perform complex information-processing tasks.
Their involvement in complex gene circuits results in sophisticated circuit topologies that are reminiscent of electronics and that are capable of providing engineered cells with the ability to memorize events, oscillate protein production, and perform complex information-processing tasks.
Complex gene circuits containing gene switches are described as capable of enabling mammalian cells to memorize events, oscillate protein production, and perform complex information-processing tasks.
Their involvement in complex gene circuits results in sophisticated circuit topologies that are reminiscent of electronics and that are capable of providing engineered cells with the ability to memorize events, oscillate protein production, and perform complex information-processing tasks.
The review states that progress in gene circuit design together with genome engineering may enable tailored engineered mammalian cells for future cell-based therapies.
Progress in gene circuit design, in combination with recent breakthroughs in genome engineering, may result in tailored engineered mammalian cells with great potential for future cell-based therapies.
The review states that progress in gene circuit design together with genome engineering may enable tailored engineered mammalian cells for future cell-based therapies.
Progress in gene circuit design, in combination with recent breakthroughs in genome engineering, may result in tailored engineered mammalian cells with great potential for future cell-based therapies.
The review states that progress in gene circuit design together with genome engineering may enable tailored engineered mammalian cells for future cell-based therapies.
Progress in gene circuit design, in combination with recent breakthroughs in genome engineering, may result in tailored engineered mammalian cells with great potential for future cell-based therapies.
The review states that progress in gene circuit design together with genome engineering may enable tailored engineered mammalian cells for future cell-based therapies.
Progress in gene circuit design, in combination with recent breakthroughs in genome engineering, may result in tailored engineered mammalian cells with great potential for future cell-based therapies.
The review states that progress in gene circuit design together with genome engineering may enable tailored engineered mammalian cells for future cell-based therapies.
Progress in gene circuit design, in combination with recent breakthroughs in genome engineering, may result in tailored engineered mammalian cells with great potential for future cell-based therapies.
The review states that progress in gene circuit design together with genome engineering may enable tailored engineered mammalian cells for future cell-based therapies.
Progress in gene circuit design, in combination with recent breakthroughs in genome engineering, may result in tailored engineered mammalian cells with great potential for future cell-based therapies.
The review states that progress in gene circuit design together with genome engineering may enable tailored engineered mammalian cells for future cell-based therapies.
Progress in gene circuit design, in combination with recent breakthroughs in genome engineering, may result in tailored engineered mammalian cells with great potential for future cell-based therapies.
Microencapsulated mammalian cells engineered with closed-loop gene networks are described as implantable in mice to sense disease-related inputs and produce fine-tuned therapeutic responses that rebalance metabolism.
Microencapsulated mammalian cells that are engineered with closed-loop gene networks can be implanted into mice to sense disease-related input signals and to process this information to produce a custom, fine-tuned therapeutic response that rebalances animal metabolism.
Microencapsulated mammalian cells engineered with closed-loop gene networks are described as implantable in mice to sense disease-related inputs and produce fine-tuned therapeutic responses that rebalance metabolism.
Microencapsulated mammalian cells that are engineered with closed-loop gene networks can be implanted into mice to sense disease-related input signals and to process this information to produce a custom, fine-tuned therapeutic response that rebalances animal metabolism.
Microencapsulated mammalian cells engineered with closed-loop gene networks are described as implantable in mice to sense disease-related inputs and produce fine-tuned therapeutic responses that rebalance metabolism.
Microencapsulated mammalian cells that are engineered with closed-loop gene networks can be implanted into mice to sense disease-related input signals and to process this information to produce a custom, fine-tuned therapeutic response that rebalances animal metabolism.
Microencapsulated mammalian cells engineered with closed-loop gene networks are described as implantable in mice to sense disease-related inputs and produce fine-tuned therapeutic responses that rebalance metabolism.
Microencapsulated mammalian cells that are engineered with closed-loop gene networks can be implanted into mice to sense disease-related input signals and to process this information to produce a custom, fine-tuned therapeutic response that rebalances animal metabolism.
Microencapsulated mammalian cells engineered with closed-loop gene networks are described as implantable in mice to sense disease-related inputs and produce fine-tuned therapeutic responses that rebalance metabolism.
Microencapsulated mammalian cells that are engineered with closed-loop gene networks can be implanted into mice to sense disease-related input signals and to process this information to produce a custom, fine-tuned therapeutic response that rebalances animal metabolism.
Microencapsulated mammalian cells engineered with closed-loop gene networks are described as implantable in mice to sense disease-related inputs and produce fine-tuned therapeutic responses that rebalance metabolism.
Microencapsulated mammalian cells that are engineered with closed-loop gene networks can be implanted into mice to sense disease-related input signals and to process this information to produce a custom, fine-tuned therapeutic response that rebalances animal metabolism.
Microencapsulated mammalian cells engineered with closed-loop gene networks are described as implantable in mice to sense disease-related inputs and produce fine-tuned therapeutic responses that rebalance metabolism.
Microencapsulated mammalian cells that are engineered with closed-loop gene networks can be implanted into mice to sense disease-related input signals and to process this information to produce a custom, fine-tuned therapeutic response that rebalances animal metabolism.
Approval Evidence
1 source1 linked approval claimfirst-pass slug genome-engineering
Progress in gene circuit design, in combination with recent breakthroughs in genome engineering
Source:
future potentialsupports
The review states that progress in gene circuit design together with genome engineering may enable tailored engineered mammalian cells for future cell-based therapies.
Progress in gene circuit design, in combination with recent breakthroughs in genome engineering, may result in tailored engineered mammalian cells with great potential for future cell-based therapies.
Source:
Comparisons
Source-backed strengths
A stated strength is its conceptual complementarity with gene circuit design in mammalian cells. The review also states that complex gene circuits with gene switches can support memory, oscillatory protein production, and information processing, but these capabilities are attributed to the circuits rather than to a defined genome engineering tool.
Ranked Citations
- 1.