Source 1primary paper2025PPR
Toolkit/HAdV-D10
HAdV-D10
Also known as: Adenovirus 10, human adenovirus type 10
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Species D adenoviruses, such as human adenovirus type 10 (HAdV-D10), are promising candidates due to low seroprevalence in humans... support the advancement of HAdV-D10 as a next-generation platform for gene delivery and vaccine development.
Usefulness & Problems
Why this is useful
HAdV-D10 is presented as an adenoviral platform candidate for gene delivery and vaccine development. The paper characterizes its capsid structure and infection-associated transcripts to inform vector development.; gene delivery; vaccine development; vector development
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HAdV-D10 is presented as an adenoviral platform candidate for gene delivery and vaccine development. The paper characterizes its capsid structure and infection-associated transcripts to inform vector development.
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gene delivery
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vaccine development
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vector development
Problem solved
It addresses the need for adenoviral vectors with favorable translational properties, including low human seroprevalence. The paper frames this as relevant for subunit vaccines and oncolytic therapies.; provides a low-seroprevalence adenoviral platform candidate for therapeutic transgene delivery
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It addresses the need for adenoviral vectors with favorable translational properties, including low human seroprevalence. The paper frames this as relevant for subunit vaccines and oncolytic therapies.
Source:
provides a low-seroprevalence adenoviral platform candidate for therapeutic transgene delivery
Problem links
Under-Provisioning of Antibiotics, Vaccines and Other Interventions for Major Global Health Challenges
Gap mapView gapThis item is explicitly described as a platform for gene delivery and vaccine development, which is directly relevant to the vaccine under-provisioning part of the gap. The noted low human seroprevalence could be advantageous for vector-based vaccination strategies.
provides a low-seroprevalence adenoviral platform candidate for therapeutic transgene delivery
LiteratureIt addresses the need for adenoviral vectors with favorable translational properties, including low human seroprevalence. The paper frames this as relevant for subunit vaccines and oncolytic therapies.
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It addresses the need for adenoviral vectors with favorable translational properties, including low human seroprevalence. The paper frames this as relevant for subunit vaccines and oncolytic therapies.
Published Workflows
Objective: To inform HAdV-D10 vector development through combined structural and transcriptomic characterization prior to clinical translation.
Why it works: The paper pairs capsid structural analysis with infected-cell transcript profiling so that both entry-relevant architecture and viral gene-expression features can be evaluated to inform vector development.
fiber shaft structural features relevant to cell entryviral transcript usage including mature protein VII-equivalent transcript expressioncryo-electron microscopytranscriptomic profilingORF-centric transcript analysis
Stages
- 1.Structural characterization of HAdV-D10 capsid and fiber shaft(functional_characterization)
This stage provides structural information about the capsid and fiber shaft, including a newly observed motif, to inform vector development.
Selection: Resolve HAdV-D10 capsid architecture and identify entry-relevant structural features.
- 2.Transcriptomic profiling of infected cells with ORF-centric analysis(secondary_characterization)
This stage identifies transcriptomic features that differ from HAdV-C5 and may matter for vector development.
Selection: Profile viral transcripts and compare HAdV-D10 transcript usage against HAdV-C5.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A delivery strategy grouped with the mechanism branch because it determines how a system is instantiated and deployed in context.
Techniques
Structural CharacterizationTarget processes
translationImplementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: externally suppliedimplementation constraint: context specific validationimplementation constraint: payload burdenoperating role: delivery
Use of HAdV-D10 as a vector requires target-cell infection and high-level therapeutic transgene delivery. The study also relies on cryo-EM and infected-cell transcript profiling to characterize it.; efficient use depends on infecting target cells and delivering therapeutic transgenes at high levels
The abstract does not show clinical performance or direct therapeutic efficacy. It instead emphasizes preclinical characterization before clinical translation.; requires detailed characterization prior to clinical translation
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The HAdV-D10 fiber shaft contains a previously uncharacterized umbrella motif resolved by cryo-EM at 4.6 Å.
The fiber shaft, essential for cell entry, was resolved at 4.6 Å, revealing a previously uncharacterized ‘umbrella’ motif.
resolution 4.6 Å
An ORF-centric pipeline identified key transcript differences between HAdV-D10 and HAdV-C5, including abundant expression of a transcript encoding a protein equivalent to mature protein VII.
Viral transcript analysis using an ORF-centric pipeline uncovered key differences from HAdV-C5, including abundant expression of a transcript encoding a protein equivalent to mature protein VII.
The reported structural and transcriptomic findings support advancement of HAdV-D10 as a next-generation platform for gene delivery and vaccine development.
These findings highlight the importance of detailed vector characterization prior to clinical translation and support the advancement of HAdV-D10 as a next-generation platform for gene delivery and vaccine development.
Approval Evidence
1 source3 linked approval claimsfirst-pass slug hadv-d10
Species D adenoviruses, such as human adenovirus type 10 (HAdV-D10), are promising candidates due to low seroprevalence in humans... support the advancement of HAdV-D10 as a next-generation platform for gene delivery and vaccine development.
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structural characterizationsupports
The HAdV-D10 fiber shaft contains a previously uncharacterized umbrella motif resolved by cryo-EM at 4.6 Å.
The fiber shaft, essential for cell entry, was resolved at 4.6 Å, revealing a previously uncharacterized ‘umbrella’ motif.
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transcriptomic differencesupports
An ORF-centric pipeline identified key transcript differences between HAdV-D10 and HAdV-C5, including abundant expression of a transcript encoding a protein equivalent to mature protein VII.
Viral transcript analysis using an ORF-centric pipeline uncovered key differences from HAdV-C5, including abundant expression of a transcript encoding a protein equivalent to mature protein VII.
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translational potentialsupports
The reported structural and transcriptomic findings support advancement of HAdV-D10 as a next-generation platform for gene delivery and vaccine development.
These findings highlight the importance of detailed vector characterization prior to clinical translation and support the advancement of HAdV-D10 as a next-generation platform for gene delivery and vaccine development.
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Comparisons
Source-stated alternatives
HAdV-C5 is the explicit comparator used for transcript differences in the abstract. More broadly, adenoviruses are described as vectors for vaccines and oncolytic therapies.
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HAdV-C5 is the explicit comparator used for transcript differences in the abstract. More broadly, adenoviruses are described as vectors for vaccines and oncolytic therapies.
Source-backed strengths
described as promising due to low seroprevalence in humans; supported by structural and transcriptomic characterization in this study
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described as promising due to low seroprevalence in humans
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supported by structural and transcriptomic characterization in this study
Compared with intranasal oxytocin
HAdV-D10 and intranasal oxytocin address a similar problem space because they share translation.
Shared frame: same top-level item type; shared target processes: translation; shared mechanisms: translation_control
Compared with optical cochlear implant
HAdV-D10 and optical cochlear implant address a similar problem space because they share translation.
Shared frame: same top-level item type; shared target processes: translation; shared mechanisms: translation_control
Strengths here: looks easier to implement in practice.
Compared with virus-like particles
HAdV-D10 and virus-like particles address a similar problem space because they share translation.
Shared frame: same top-level item type; shared target processes: translation; shared mechanisms: translation_control
Relative tradeoffs: appears more independently replicated; looks easier to implement in practice.
Ranked Citations
- 1.