Source 1primary paper2019Journal of Visualized Experiments
Toolkit/high-viscosity micro-extrusion injector
high-viscosity micro-extrusion injector
Also known as: high-viscosity extruder
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
High-viscosity micro-extrusion injectors have dramatically reduced sample consumption in serial femtosecond crystallographic experiments (SFX) at X-ray free electron lasers (XFELs).
Usefulness & Problems
No literature-backed usefulness or problem-fit explainer has been materialized for this record yet.
Published Workflows
Objective: Optimize extrusion of bacteriorhodopsin microcrystals for time-resolved serial femtosecond crystallography by achieving stable continuous flow while maintaining high crystal density and ensuring crystal clearance between pump laser pulses.
Why it works: The workflow first prepares lipidic cubic phase with a homogeneous crystal distribution and then adjusts sample composition using measured extrusion stability, which the abstract presents as the route to stable continuous flow at high crystal density.
homogeneous distribution of crystals in lipidic cubic phasesample composition adjustment to improve extrusion stabilityhigh-viscosity micro-extrusionthree-way syringe coupling preparationhigh-speed camera-based extrusion stability measurement
Stages
- 1.Prepare lipidic cubic phase with homogeneous crystal distribution(library_build)
This stage creates lipidic cubic phase with a homogeneous distribution of crystals before extrusion optimization.
Selection: homogeneous distribution of crystals in lipidic cubic phase
- 2.Measure extrusion stability and adjust sample composition(functional_characterization)
This stage uses high-speed camera measurements of extrusion stability to guide sample composition adjustment for optimized flow.
Selection: measurements of extrusion stability
Steps
- 1.Prepare lipidic cubic phase with homogeneous crystal distribution using the three-way syringe coupling devicesample-preparation device
Generate lipidic cubic phase with homogeneous crystal distribution before extrusion testing.
Homogeneous crystal distribution is prepared first because it is part of how the method achieves optimized extrusion.
- 2.Measure extrusion stability with a high-speed camera setupmeasurement setup
Obtain extrusion stability measurements to inform sample composition adjustment.
This measurement is performed before composition adjustment because the adjustment is based on the measured extrusion stability.
- 3.Adjust sample composition based on extrusion stability measurements
Tune the sample for stable continuous flow while maintaining high crystal density.
This step follows the measurement step because the composition adjustment is explicitly based on the measured extrusion stability.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A delivery strategy grouped with the mechanism branch because it determines how a system is instantiated and deployed in context.
Techniques
Structural CharacterizationTarget processes
recombinationInput: Light
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The described methodology can be adapted to optimize the flow of other microcrystals.
The methodology can be adapted to optimize the flow of other microcrystals.
The method aims to optimize extrusion for stable continuous flow while maintaining high crystal density to increase TR-SFX data collection rate.
The goal of the method is to optimize extrusion for a stable and continuous flow while maintaining a high density of crystals to increase the rate at which data can be collected in a TR-SFX experiment.
High-viscosity micro-extrusion injectors dramatically reduce sample consumption in serial femtosecond crystallography experiments at XFELs.
High-viscosity micro-extrusion injectors have dramatically reduced sample consumption in serial femtosecond crystallographic experiments (SFX) at X-ray free electron lasers (XFELs).
High-viscosity micro-extrusion injectors are a preferred option for delivering crystals for time-resolved serial femtosecond crystallography in bacteriorhodopsin experiments.
A series of experiments using the light-driven proton pump bacteriorhodopsin have further established these injectors as a preferred option to deliver crystals for time-resolved serial femtosecond crystallography (TR-SFX) to resolve structural changes of proteins after photoactivation.
A high-speed camera setup is used to measure extrusion stability and guide sample composition adjustment.
followed by adjusting the sample composition based on measurements of the extrusion stability taken with a high-speed camera setup
A three-way syringe coupling device is used to prepare lipidic cubic phase with a homogeneous distribution of crystals.
We achieve this goal by preparing lipidic cubic phase with a homogenous distribution of crystals using a novel three-way syringe coupling device
Approval Evidence
1 source4 linked approval claimsfirst-pass slug high-viscosity-micro-extrusion-injector
High-viscosity micro-extrusion injectors have dramatically reduced sample consumption in serial femtosecond crystallographic experiments (SFX) at X-ray free electron lasers (XFELs).
Source:
generalizabilitysupports
The described methodology can be adapted to optimize the flow of other microcrystals.
The methodology can be adapted to optimize the flow of other microcrystals.
Source:
method objectivesupports
The method aims to optimize extrusion for stable continuous flow while maintaining high crystal density to increase TR-SFX data collection rate.
The goal of the method is to optimize extrusion for a stable and continuous flow while maintaining a high density of crystals to increase the rate at which data can be collected in a TR-SFX experiment.
Source:
performance advantagesupports
High-viscosity micro-extrusion injectors dramatically reduce sample consumption in serial femtosecond crystallography experiments at XFELs.
High-viscosity micro-extrusion injectors have dramatically reduced sample consumption in serial femtosecond crystallographic experiments (SFX) at X-ray free electron lasers (XFELs).
Source:
preferred use casesupports
High-viscosity micro-extrusion injectors are a preferred option for delivering crystals for time-resolved serial femtosecond crystallography in bacteriorhodopsin experiments.
A series of experiments using the light-driven proton pump bacteriorhodopsin have further established these injectors as a preferred option to deliver crystals for time-resolved serial femtosecond crystallography (TR-SFX) to resolve structural changes of proteins after photoactivation.
Source:
Comparisons
No literature-backed comparison notes have been materialized for this record yet.
Ranked Citations
- 1.