Toolkit/human Neuropsin

human Neuropsin

Multi-Component Switch·Research·Since 2022

Also known as: hOPN5

Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.

Summary

Human Neuropsin (hOPN5) is a light-responsive optogenetic switch used to selectively control Gq signaling. It has been reported to support specific and repetitive manipulation of this pathway in vitro and in vivo with high spatiotemporal resolution.

Usefulness & Problems

Why this is useful

hOPN5 is useful for optical control of Gq-coupled signaling with temporal and spatial precision. The cited study also reports its use in an all-optical high-throughput screening context for TRPC6 inhibitors, where screening was described as more specific and sensitive than conventional pharmacological screening.

Source:

Here we present the human Neuropsin (hOPN5) for specific and repetitive manipulation of Gq signaling in vitro and in vivo with high spatio-temporal resolution.

Problem solved

hOPN5 addresses the need for selective manipulation of Gq signaling without relying solely on conventional pharmacological perturbation. It also supports optical assay formats for screening compounds affecting downstream Gq-linked effectors such as TRPC6.

Taxonomy & Function

Primary hierarchy

Mechanism Branch

Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.

Target processes

recombinationselectionsignaling

Input: Light

Implementation Constraints

The available evidence establishes that hOPN5 is actuated by light and used as an optogenetic tool for Gq pathway control in vitro and in vivo. However, the provided material does not specify wavelength, expression strategy, required cofactors, delivery modality, or fusion/construct design details.

The supplied evidence does not provide construct architecture, illumination parameters, chromophore requirements, kinetic values, or quantitative dynamic range. Independent replication is not documented in the provided material, and the evidence is centered on a single 2022 study.

Validation

Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication

Supporting Sources

Ranked Claims

Claim 1functional capabilitysupports2022Source 1needs review

Human Neuropsin (hOPN5) enables specific and repetitive manipulation of Gq signaling in vitro and in vivo with high spatio-temporal resolution.

Here we present the human Neuropsin (hOPN5) for specific and repetitive manipulation of Gq signaling in vitro and in vivo with high spatio-temporal resolution.
Claim 2functional capabilitysupports2022Source 1needs review

Human Neuropsin (hOPN5) enables specific and repetitive manipulation of Gq signaling in vitro and in vivo with high spatio-temporal resolution.

Here we present the human Neuropsin (hOPN5) for specific and repetitive manipulation of Gq signaling in vitro and in vivo with high spatio-temporal resolution.
Claim 3functional capabilitysupports2022Source 1needs review

Human Neuropsin (hOPN5) enables specific and repetitive manipulation of Gq signaling in vitro and in vivo with high spatio-temporal resolution.

Here we present the human Neuropsin (hOPN5) for specific and repetitive manipulation of Gq signaling in vitro and in vivo with high spatio-temporal resolution.
Claim 4functional capabilitysupports2022Source 1needs review

Human Neuropsin (hOPN5) enables specific and repetitive manipulation of Gq signaling in vitro and in vivo with high spatio-temporal resolution.

Here we present the human Neuropsin (hOPN5) for specific and repetitive manipulation of Gq signaling in vitro and in vivo with high spatio-temporal resolution.
Claim 5functional capabilitysupports2022Source 1needs review

Human Neuropsin (hOPN5) enables specific and repetitive manipulation of Gq signaling in vitro and in vivo with high spatio-temporal resolution.

Here we present the human Neuropsin (hOPN5) for specific and repetitive manipulation of Gq signaling in vitro and in vivo with high spatio-temporal resolution.
Claim 6functional capabilitysupports2022Source 1needs review

Human Neuropsin (hOPN5) enables specific and repetitive manipulation of Gq signaling in vitro and in vivo with high spatio-temporal resolution.

Here we present the human Neuropsin (hOPN5) for specific and repetitive manipulation of Gq signaling in vitro and in vivo with high spatio-temporal resolution.
Claim 7functional capabilitysupports2022Source 1needs review

Human Neuropsin (hOPN5) enables specific and repetitive manipulation of Gq signaling in vitro and in vivo with high spatio-temporal resolution.

Here we present the human Neuropsin (hOPN5) for specific and repetitive manipulation of Gq signaling in vitro and in vivo with high spatio-temporal resolution.
Claim 8screening performancesupports2022Source 1needs review

All-optical high-throughput screening for TRPC6 inhibitors is more specific and sensitive than conventional pharmacological screening.

All-optical high-throughput screening for TRPC6 inhibitors is more specific and sensitive than conventional pharmacological screening.
Claim 9screening performancesupports2022Source 1needs review

All-optical high-throughput screening for TRPC6 inhibitors is more specific and sensitive than conventional pharmacological screening.

All-optical high-throughput screening for TRPC6 inhibitors is more specific and sensitive than conventional pharmacological screening.
Claim 10screening performancesupports2022Source 1needs review

All-optical high-throughput screening for TRPC6 inhibitors is more specific and sensitive than conventional pharmacological screening.

All-optical high-throughput screening for TRPC6 inhibitors is more specific and sensitive than conventional pharmacological screening.
Claim 11screening performancesupports2022Source 1needs review

All-optical high-throughput screening for TRPC6 inhibitors is more specific and sensitive than conventional pharmacological screening.

All-optical high-throughput screening for TRPC6 inhibitors is more specific and sensitive than conventional pharmacological screening.
Claim 12screening performancesupports2022Source 1needs review

All-optical high-throughput screening for TRPC6 inhibitors is more specific and sensitive than conventional pharmacological screening.

All-optical high-throughput screening for TRPC6 inhibitors is more specific and sensitive than conventional pharmacological screening.
Claim 13screening performancesupports2022Source 1needs review

All-optical high-throughput screening for TRPC6 inhibitors is more specific and sensitive than conventional pharmacological screening.

All-optical high-throughput screening for TRPC6 inhibitors is more specific and sensitive than conventional pharmacological screening.
Claim 14screening performancesupports2022Source 1needs review

All-optical high-throughput screening for TRPC6 inhibitors is more specific and sensitive than conventional pharmacological screening.

All-optical high-throughput screening for TRPC6 inhibitors is more specific and sensitive than conventional pharmacological screening.
Claim 15signaling specificitysupports2022Source 1needs review

hOPN5 shows specific Gq signaling.

Thus, we demonstrate specific Gq signaling of hOPN5 and unveil its potential for optogenetic applications.
Claim 16signaling specificitysupports2022Source 1needs review

hOPN5 shows specific Gq signaling.

Thus, we demonstrate specific Gq signaling of hOPN5 and unveil its potential for optogenetic applications.
Claim 17signaling specificitysupports2022Source 1needs review

hOPN5 shows specific Gq signaling.

Thus, we demonstrate specific Gq signaling of hOPN5 and unveil its potential for optogenetic applications.
Claim 18signaling specificitysupports2022Source 1needs review

hOPN5 shows specific Gq signaling.

Thus, we demonstrate specific Gq signaling of hOPN5 and unveil its potential for optogenetic applications.
Claim 19signaling specificitysupports2022Source 1needs review

hOPN5 shows specific Gq signaling.

Thus, we demonstrate specific Gq signaling of hOPN5 and unveil its potential for optogenetic applications.
Claim 20signaling specificitysupports2022Source 1needs review

hOPN5 shows specific Gq signaling.

Thus, we demonstrate specific Gq signaling of hOPN5 and unveil its potential for optogenetic applications.
Claim 21signaling specificitysupports2022Source 1needs review

hOPN5 shows specific Gq signaling.

Thus, we demonstrate specific Gq signaling of hOPN5 and unveil its potential for optogenetic applications.

Approval Evidence

1 source3 linked approval claimsfirst-pass slug human-neuropsin
Here we present the human Neuropsin (hOPN5) for specific and repetitive manipulation of Gq signaling in vitro and in vivo with high spatio-temporal resolution.

Source:

functional capabilitysupports

Human Neuropsin (hOPN5) enables specific and repetitive manipulation of Gq signaling in vitro and in vivo with high spatio-temporal resolution.

Here we present the human Neuropsin (hOPN5) for specific and repetitive manipulation of Gq signaling in vitro and in vivo with high spatio-temporal resolution.

Source:

screening performancesupports

All-optical high-throughput screening for TRPC6 inhibitors is more specific and sensitive than conventional pharmacological screening.

All-optical high-throughput screening for TRPC6 inhibitors is more specific and sensitive than conventional pharmacological screening.

Source:

signaling specificitysupports

hOPN5 shows specific Gq signaling.

Thus, we demonstrate specific Gq signaling of hOPN5 and unveil its potential for optogenetic applications.

Source:

Comparisons

Source-backed strengths

The reported strengths are signaling specificity for Gq and the ability to drive repetitive manipulations with high spatiotemporal resolution. Validation was described in both in vitro and in vivo settings, and the associated all-optical screening application was reported to outperform conventional pharmacological screening in specificity and sensitivity.

Ranked Citations

  1. 1.
    StructuralSource 1Nature Communications2022Claim 1Claim 2Claim 3

    Extracted from this source document.