Source 1primary paper2022
Toolkit/I-BAR domain
I-BAR domain
Taxonomy: Mechanism Branch / Component. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The I-BAR domain is used as a component of CRY-BARs, a family of light-gated I-BAR-domain-containing tools designed for remodeling membrane architecture. In this context, the domain contributes to optogenetic control of membrane-associated cellular dynamics and membrane protrusion-related processes.
Usefulness & Problems
Why this is useful
Within CRY-BARs, the I-BAR domain is useful for building light-responsive tools that remodel membrane architectures and control cellular dynamics. The reported applications include use in cell lines and primary neuron cultures to report membrane dynamic changes associated with cellular activity.
Source:
with applications in the remodeling of membrane architectures and the control of cellular dynamics
Source:
Using cell lines and primary neuron cultures, we demonstrate that the CRY-BAR optogenetic tool reports membrane dynamic changes associated with cellular activity.
Problem solved
The I-BAR domain helps enable construction of light-gated tools for spatial and temporal control of membrane architecture remodeling. The cited work specifically positions these tools to address experimental needs involving induction and observation of membrane dynamic changes in living cells.
Source:
with applications in the remodeling of membrane architectures and the control of cellular dynamics
Source:
Using cell lines and primary neuron cultures, we demonstrate that the CRY-BAR optogenetic tool reports membrane dynamic changes associated with cellular activity.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Component: A low-level protein part used inside a larger architecture that realizes a mechanism.
Techniques
Computational DesignTarget processes
No target processes tagged yet.
Input: Light
Implementation Constraints
The reported implementation is through design of light-gated I-BAR-domain-containing fusion tools termed CRY-BARs. Beyond the fact that these are light-gated and were used in cell lines and primary neuron cultures, the supplied evidence does not provide additional practical details on cofactors, expression systems, or construct configuration.
The supplied evidence concerns the I-BAR domain only in the context of CRY-BAR fusion tools rather than as a standalone domain. The source text provided here does not specify quantitative performance, illumination parameters, construct architecture details, or direct independent replication.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
CRY-BARs have applications in remodeling membrane architectures and controlling cellular dynamics.
with applications in the remodeling of membrane architectures and the control of cellular dynamics
CRY-BARs have applications in remodeling membrane architectures and controlling cellular dynamics.
with applications in the remodeling of membrane architectures and the control of cellular dynamics
CRY-BARs have applications in remodeling membrane architectures and controlling cellular dynamics.
with applications in the remodeling of membrane architectures and the control of cellular dynamics
CRY-BARs have applications in remodeling membrane architectures and controlling cellular dynamics.
with applications in the remodeling of membrane architectures and the control of cellular dynamics
CRY-BARs have applications in remodeling membrane architectures and controlling cellular dynamics.
with applications in the remodeling of membrane architectures and the control of cellular dynamics
CRY-BARs have applications in remodeling membrane architectures and controlling cellular dynamics.
with applications in the remodeling of membrane architectures and the control of cellular dynamics
CRY-BARs have applications in remodeling membrane architectures and controlling cellular dynamics.
with applications in the remodeling of membrane architectures and the control of cellular dynamics
In cell lines and primary neuron cultures, the CRY-BAR optogenetic tool reports membrane dynamic changes associated with cellular activity.
Using cell lines and primary neuron cultures, we demonstrate that the CRY-BAR optogenetic tool reports membrane dynamic changes associated with cellular activity.
In cell lines and primary neuron cultures, the CRY-BAR optogenetic tool reports membrane dynamic changes associated with cellular activity.
Using cell lines and primary neuron cultures, we demonstrate that the CRY-BAR optogenetic tool reports membrane dynamic changes associated with cellular activity.
In cell lines and primary neuron cultures, the CRY-BAR optogenetic tool reports membrane dynamic changes associated with cellular activity.
Using cell lines and primary neuron cultures, we demonstrate that the CRY-BAR optogenetic tool reports membrane dynamic changes associated with cellular activity.
In cell lines and primary neuron cultures, the CRY-BAR optogenetic tool reports membrane dynamic changes associated with cellular activity.
Using cell lines and primary neuron cultures, we demonstrate that the CRY-BAR optogenetic tool reports membrane dynamic changes associated with cellular activity.
In cell lines and primary neuron cultures, the CRY-BAR optogenetic tool reports membrane dynamic changes associated with cellular activity.
Using cell lines and primary neuron cultures, we demonstrate that the CRY-BAR optogenetic tool reports membrane dynamic changes associated with cellular activity.
In cell lines and primary neuron cultures, the CRY-BAR optogenetic tool reports membrane dynamic changes associated with cellular activity.
Using cell lines and primary neuron cultures, we demonstrate that the CRY-BAR optogenetic tool reports membrane dynamic changes associated with cellular activity.
In cell lines and primary neuron cultures, the CRY-BAR optogenetic tool reports membrane dynamic changes associated with cellular activity.
Using cell lines and primary neuron cultures, we demonstrate that the CRY-BAR optogenetic tool reports membrane dynamic changes associated with cellular activity.
The paper describes the design and implementation of a family of versatile light-gated I-BAR-domain-containing tools called CRY-BARs.
In this work, we describe the design and implementation of a family of versatile light-gated I-BAR domain containing tools (‘CRY-BARs’)
The paper describes the design and implementation of a family of versatile light-gated I-BAR-domain-containing tools called CRY-BARs.
In this work, we describe the design and implementation of a family of versatile light-gated I-BAR domain containing tools (‘CRY-BARs’)
The paper describes the design and implementation of a family of versatile light-gated I-BAR-domain-containing tools called CRY-BARs.
In this work, we describe the design and implementation of a family of versatile light-gated I-BAR domain containing tools (‘CRY-BARs’)
The paper describes the design and implementation of a family of versatile light-gated I-BAR-domain-containing tools called CRY-BARs.
In this work, we describe the design and implementation of a family of versatile light-gated I-BAR domain containing tools (‘CRY-BARs’)
The paper describes the design and implementation of a family of versatile light-gated I-BAR-domain-containing tools called CRY-BARs.
In this work, we describe the design and implementation of a family of versatile light-gated I-BAR domain containing tools (‘CRY-BARs’)
The paper describes the design and implementation of a family of versatile light-gated I-BAR-domain-containing tools called CRY-BARs.
In this work, we describe the design and implementation of a family of versatile light-gated I-BAR domain containing tools (‘CRY-BARs’)
The paper describes the design and implementation of a family of versatile light-gated I-BAR-domain-containing tools called CRY-BARs.
In this work, we describe the design and implementation of a family of versatile light-gated I-BAR domain containing tools (‘CRY-BARs’)
CRY-BARs can be used for spatial and temporal control of cellular processes that require induction of membrane protrusions by leveraging the intrinsic membrane-binding propensity of the I-BAR domain.
By taking advantage of the intrinsic membrane binding propensity of the I-BAR domain, CRY-BARs can be used for spatial and temporal control of cellular processes that require induction of membrane protrusions.
CRY-BARs can be used for spatial and temporal control of cellular processes that require induction of membrane protrusions by leveraging the intrinsic membrane-binding propensity of the I-BAR domain.
By taking advantage of the intrinsic membrane binding propensity of the I-BAR domain, CRY-BARs can be used for spatial and temporal control of cellular processes that require induction of membrane protrusions.
CRY-BARs can be used for spatial and temporal control of cellular processes that require induction of membrane protrusions by leveraging the intrinsic membrane-binding propensity of the I-BAR domain.
By taking advantage of the intrinsic membrane binding propensity of the I-BAR domain, CRY-BARs can be used for spatial and temporal control of cellular processes that require induction of membrane protrusions.
CRY-BARs can be used for spatial and temporal control of cellular processes that require induction of membrane protrusions by leveraging the intrinsic membrane-binding propensity of the I-BAR domain.
By taking advantage of the intrinsic membrane binding propensity of the I-BAR domain, CRY-BARs can be used for spatial and temporal control of cellular processes that require induction of membrane protrusions.
CRY-BARs can be used for spatial and temporal control of cellular processes that require induction of membrane protrusions by leveraging the intrinsic membrane-binding propensity of the I-BAR domain.
By taking advantage of the intrinsic membrane binding propensity of the I-BAR domain, CRY-BARs can be used for spatial and temporal control of cellular processes that require induction of membrane protrusions.
CRY-BARs can be used for spatial and temporal control of cellular processes that require induction of membrane protrusions by leveraging the intrinsic membrane-binding propensity of the I-BAR domain.
By taking advantage of the intrinsic membrane binding propensity of the I-BAR domain, CRY-BARs can be used for spatial and temporal control of cellular processes that require induction of membrane protrusions.
CRY-BARs can be used for spatial and temporal control of cellular processes that require induction of membrane protrusions by leveraging the intrinsic membrane-binding propensity of the I-BAR domain.
By taking advantage of the intrinsic membrane binding propensity of the I-BAR domain, CRY-BARs can be used for spatial and temporal control of cellular processes that require induction of membrane protrusions.
Ezrin acts as a relay between the plasma membrane and the actin cytoskeleton and is an important mediator of CRY-BAR switch function.
Moreover, we provide evidence that Ezrin acts as a relay between the plasma membrane and the actin cytoskeleton and therefore is an important mediator of switch function.
Ezrin acts as a relay between the plasma membrane and the actin cytoskeleton and is an important mediator of CRY-BAR switch function.
Moreover, we provide evidence that Ezrin acts as a relay between the plasma membrane and the actin cytoskeleton and therefore is an important mediator of switch function.
Ezrin acts as a relay between the plasma membrane and the actin cytoskeleton and is an important mediator of CRY-BAR switch function.
Moreover, we provide evidence that Ezrin acts as a relay between the plasma membrane and the actin cytoskeleton and therefore is an important mediator of switch function.
Ezrin acts as a relay between the plasma membrane and the actin cytoskeleton and is an important mediator of CRY-BAR switch function.
Moreover, we provide evidence that Ezrin acts as a relay between the plasma membrane and the actin cytoskeleton and therefore is an important mediator of switch function.
Ezrin acts as a relay between the plasma membrane and the actin cytoskeleton and is an important mediator of CRY-BAR switch function.
Moreover, we provide evidence that Ezrin acts as a relay between the plasma membrane and the actin cytoskeleton and therefore is an important mediator of switch function.
Ezrin acts as a relay between the plasma membrane and the actin cytoskeleton and is an important mediator of CRY-BAR switch function.
Moreover, we provide evidence that Ezrin acts as a relay between the plasma membrane and the actin cytoskeleton and therefore is an important mediator of switch function.
Ezrin acts as a relay between the plasma membrane and the actin cytoskeleton and is an important mediator of CRY-BAR switch function.
Moreover, we provide evidence that Ezrin acts as a relay between the plasma membrane and the actin cytoskeleton and therefore is an important mediator of switch function.
CRY-BARs hold promise as a useful addition to the optogenetic toolkit for studying membrane remodeling in live cells.
Overall, CRY-BARs hold promise as a useful addition to the optogenetic toolkit to study membrane remodeling in live cells.
CRY-BARs hold promise as a useful addition to the optogenetic toolkit for studying membrane remodeling in live cells.
Overall, CRY-BARs hold promise as a useful addition to the optogenetic toolkit to study membrane remodeling in live cells.
CRY-BARs hold promise as a useful addition to the optogenetic toolkit for studying membrane remodeling in live cells.
Overall, CRY-BARs hold promise as a useful addition to the optogenetic toolkit to study membrane remodeling in live cells.
CRY-BARs hold promise as a useful addition to the optogenetic toolkit for studying membrane remodeling in live cells.
Overall, CRY-BARs hold promise as a useful addition to the optogenetic toolkit to study membrane remodeling in live cells.
CRY-BARs hold promise as a useful addition to the optogenetic toolkit for studying membrane remodeling in live cells.
Overall, CRY-BARs hold promise as a useful addition to the optogenetic toolkit to study membrane remodeling in live cells.
CRY-BARs hold promise as a useful addition to the optogenetic toolkit for studying membrane remodeling in live cells.
Overall, CRY-BARs hold promise as a useful addition to the optogenetic toolkit to study membrane remodeling in live cells.
CRY-BARs hold promise as a useful addition to the optogenetic toolkit for studying membrane remodeling in live cells.
Overall, CRY-BARs hold promise as a useful addition to the optogenetic toolkit to study membrane remodeling in live cells.
Approval Evidence
1 source2 linked approval claimsfirst-pass slug i-bar-domain
light-gated I-BAR domain containing tools
Source:
design and implementationsupports
The paper describes the design and implementation of a family of versatile light-gated I-BAR-domain-containing tools called CRY-BARs.
In this work, we describe the design and implementation of a family of versatile light-gated I-BAR domain containing tools (‘CRY-BARs’)
Source:
mechanism or functionsupports
CRY-BARs can be used for spatial and temporal control of cellular processes that require induction of membrane protrusions by leveraging the intrinsic membrane-binding propensity of the I-BAR domain.
By taking advantage of the intrinsic membrane binding propensity of the I-BAR domain, CRY-BARs can be used for spatial and temporal control of cellular processes that require induction of membrane protrusions.
Source:
Comparisons
Source-backed strengths
The available evidence supports that I-BAR-containing CRY-BARs are versatile light-gated tools for membrane architecture remodeling. They were demonstrated in both cell lines and primary neuron cultures, indicating use across more than one cellular context.
Ranked Citations
- 1.