Source 1primary paper2016Scientific Reports
Toolkit/iC1C2
iC1C2
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
PMC text for the anchor paper explicitly reports behavioral inhibition during blue-light illumination in iC1C2+ mice and compares its effects against SwiChR and controls.
Usefulness & Problems
Why this is useful
iC1C2 is an inhibitory opsin used in the study as an optogenetic pain-inhibition tool. The summary indicates it produced behavioral inhibition during blue-light illumination.; optogenetic inhibition of pain during illumination; comparison against sustained inhibitory opsins
Source:
iC1C2 is an inhibitory opsin used in the study as an optogenetic pain-inhibition tool. The summary indicates it produced behavioral inhibition during blue-light illumination.
Source:
optogenetic inhibition of pain during illumination
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comparison against sustained inhibitory opsins
Problem solved
It enables optical suppression of pain-related behavior while light is applied.; provides light-dependent inhibition of peripheral pain signaling
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It enables optical suppression of pain-related behavior while light is applied.
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provides light-dependent inhibition of peripheral pain signaling
Problem links
provides light-dependent inhibition of peripheral pain signaling
LiteratureIt enables optical suppression of pain-related behavior while light is applied.
Source:
It enables optical suppression of pain-related behavior while light is applied.
Published Workflows
Objective: Develop and apply optogenetic and chemogenetic strategies for sustained inhibition of pain through peripheral nociceptor control.
Why it works: The study combines peripheral viral delivery of inhibitory actuators with in vivo behavioral testing, allowing direct comparison of optical and ligand-gated inhibition strategies in pain-related afferents.
optogenetic inhibition of peripheral nociceptorschemogenetic inhibition of peripheral nociceptorsAAV-mediated peripheral deliveryin vivo behavioral assay
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Mechanisms
light-gated optogenetic inhibitionTechniques
No technique tags yet.
Target processes
No target processes tagged yet.
Input: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: payload burdenimplementation constraint: spectral hardware requirementoperating role: actuator
The study context supports viral expression in peripheral nociceptors and blue-light delivery.; requires expression in peripheral afferents; requires blue-light illumination
The provided evidence does not support sustained post-light inhibition for iC1C2 in the way reported for SwiChR.; effect described during illumination rather than explicitly sustained after light offset
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The study used optogenetic and chemogenetic strategies in peripheral nociceptors to achieve sustained inhibition of pain.
The study developed optoPAIN to examine bidirectional optogenetic and chemogenetic control of pain without physically contacting the animal.
AAV6-hSyn delivery was used to express inhibitory optogenetic and chemogenetic constructs in peripheral afferents.
hM4D(Gi) expression in peripheral afferents increased mechanical and thermal thresholds in a CNO-dependent manner.
iC1C2 produced behavioral inhibition during blue-light illumination in the study.
SwiChR enabled transdermal optogenetic inhibition with sustained post-light inhibition of pain behaviors.
Approval Evidence
1 source2 linked approval claimsfirst-pass slug ic1c2
PMC text for the anchor paper explicitly reports behavioral inhibition during blue-light illumination in iC1C2+ mice and compares its effects against SwiChR and controls.
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applicationsupports
The study used optogenetic and chemogenetic strategies in peripheral nociceptors to achieve sustained inhibition of pain.
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mechanism or performancesupports
iC1C2 produced behavioral inhibition during blue-light illumination in the study.
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Comparisons
Source-stated alternatives
The paper contrasts iC1C2 with SwiChR and with the chemogenetic receptor hM4D(Gi).
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The paper contrasts iC1C2 with SwiChR and with the chemogenetic receptor hM4D(Gi).
Source-backed strengths
reported behavioral inhibition during blue-light illumination
Source:
reported behavioral inhibition during blue-light illumination
Compared with chemogenetic circuit manipulation
The paper contrasts iC1C2 with SwiChR and with the chemogenetic receptor hM4D(Gi).
Shared frame: source-stated alternative in extracted literature
Strengths here: reported behavioral inhibition during blue-light illumination.
Relative tradeoffs: effect described during illumination rather than explicitly sustained after light offset.
Source:
The paper contrasts iC1C2 with SwiChR and with the chemogenetic receptor hM4D(Gi).
Compared with hM4D(Gi)
The paper contrasts iC1C2 with SwiChR and with the chemogenetic receptor hM4D(Gi).
Shared frame: source-stated alternative in extracted literature
Strengths here: reported behavioral inhibition during blue-light illumination.
Relative tradeoffs: effect described during illumination rather than explicitly sustained after light offset.
Source:
The paper contrasts iC1C2 with SwiChR and with the chemogenetic receptor hM4D(Gi).
Compared with SwiChR
The paper contrasts iC1C2 with SwiChR and with the chemogenetic receptor hM4D(Gi).
Shared frame: source-stated alternative in extracted literature
Strengths here: reported behavioral inhibition during blue-light illumination.
Relative tradeoffs: effect described during illumination rather than explicitly sustained after light offset.
Source:
The paper contrasts iC1C2 with SwiChR and with the chemogenetic receptor hM4D(Gi).
Ranked Citations
- 1.