Source 1primary paper2020Physical Review X
Toolkit/iLID-nano
iLID-nano
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
iLID-nano is a multi-component optogenetic switch built from an improved light-induced dimerization pair comprising LOV2-SsrA and SspB. It has been used to control talin-mediated cell spreading and migration and has been physically characterized for force-coupled regulation in mechanotransduction contexts.
Usefulness & Problems
Why this is useful
This tool is useful for optically controlling protein association in cellular systems where mechanical load is relevant. Its quantified ability to remain stable under forces up to 10 pN for seconds to tens of seconds supports use in mechanotransduction studies involving similar force regimes.
Source:
We demonstrate the use of this system to control talin-mediated cell spreading and migration.
Source:
The mechanical stability of the system suggests that it can be employed to modulate mechanotransduction processes that involve similar force ranges.
Problem solved
iLID-nano helps address the problem of modulating signaling or adhesion-linked processes with a light-responsive interaction module that remains functional under physiologically relevant mechanical tension. The cited work specifically positions it for controlling talin-mediated cell spreading and migration and for probing force-coupled mechanotransduction.
Source:
We demonstrate the use of this system to control talin-mediated cell spreading and migration.
Source:
The mechanical stability of the system suggests that it can be employed to modulate mechanotransduction processes that involve similar force ranges.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Techniques
No technique tags yet.
Target processes
recombinationInput: Light
Implementation Constraints
The switch is composed of LOV2-SsrA and SspB, so implementation requires expression or delivery of these interacting components as a multi-component system. The available evidence supports use in talin-mediated cell spreading and migration assays, but it does not provide further construct design, cofactor, or host-system details.
The supplied evidence is focused on mechanotransduction-related use and mechanical characterization rather than broad benchmarking across many targets or organisms. Stability decreases with increasing force, and no additional implementation details such as wavelength, kinetics, expression context, or independent replication are provided in the supplied evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Observations
successMammalian Cell Lineapplication demo
Inferred from claim c3 during normalization. The iLID-nano system was used to control talin-mediated cell spreading and migration. Derived from claim c3. Quoted text: We demonstrate the use of this system to control talin-mediated cell spreading and migration.
Source:
successMammalian Cell Lineapplication demo
Inferred from claim c4 during normalization. This work establishes a physical basis for using iLID-nano to directly control intramolecular force transmission in cells during mechanotransduction. Derived from claim c4. Quoted text: Together, we establish the physical basis for utilizing the iLID-nano system in the direct control of intramolecular force transmission in cells during mechanotransduction processes.
Source:
successMammalian Cell Lineapplication demo
Inferred from claim c3 during normalization. The iLID-nano system was used to control talin-mediated cell spreading and migration. Derived from claim c3. Quoted text: We demonstrate the use of this system to control talin-mediated cell spreading and migration.
Source:
successMammalian Cell Lineapplication demo
Inferred from claim c4 during normalization. This work establishes a physical basis for using iLID-nano to directly control intramolecular force transmission in cells during mechanotransduction. Derived from claim c4. Quoted text: Together, we establish the physical basis for utilizing the iLID-nano system in the direct control of intramolecular force transmission in cells during mechanotransduction processes.
Source:
successMammalian Cell Lineapplication demo
Inferred from claim c3 during normalization. The iLID-nano system was used to control talin-mediated cell spreading and migration. Derived from claim c3. Quoted text: We demonstrate the use of this system to control talin-mediated cell spreading and migration.
Source:
successMammalian Cell Lineapplication demo
Inferred from claim c4 during normalization. This work establishes a physical basis for using iLID-nano to directly control intramolecular force transmission in cells during mechanotransduction. Derived from claim c4. Quoted text: Together, we establish the physical basis for utilizing the iLID-nano system in the direct control of intramolecular force transmission in cells during mechanotransduction processes.
Source:
successMammalian Cell Lineapplication demo
Inferred from claim c3 during normalization. The iLID-nano system was used to control talin-mediated cell spreading and migration. Derived from claim c3. Quoted text: We demonstrate the use of this system to control talin-mediated cell spreading and migration.
Source:
successMammalian Cell Lineapplication demo
Inferred from claim c4 during normalization. This work establishes a physical basis for using iLID-nano to directly control intramolecular force transmission in cells during mechanotransduction. Derived from claim c4. Quoted text: Together, we establish the physical basis for utilizing the iLID-nano system in the direct control of intramolecular force transmission in cells during mechanotransduction processes.
Source:
successMammalian Cell Lineapplication demo
Inferred from claim c3 during normalization. The iLID-nano system was used to control talin-mediated cell spreading and migration. Derived from claim c3. Quoted text: We demonstrate the use of this system to control talin-mediated cell spreading and migration.
Source:
successMammalian Cell Lineapplication demo
Inferred from claim c4 during normalization. This work establishes a physical basis for using iLID-nano to directly control intramolecular force transmission in cells during mechanotransduction. Derived from claim c4. Quoted text: Together, we establish the physical basis for utilizing the iLID-nano system in the direct control of intramolecular force transmission in cells during mechanotransduction processes.
Source:
successMammalian Cell Lineapplication demo
Inferred from claim c3 during normalization. The iLID-nano system was used to control talin-mediated cell spreading and migration. Derived from claim c3. Quoted text: We demonstrate the use of this system to control talin-mediated cell spreading and migration.
Source:
successMammalian Cell Lineapplication demo
Inferred from claim c4 during normalization. This work establishes a physical basis for using iLID-nano to directly control intramolecular force transmission in cells during mechanotransduction. Derived from claim c4. Quoted text: Together, we establish the physical basis for utilizing the iLID-nano system in the direct control of intramolecular force transmission in cells during mechanotransduction processes.
Source:
successMammalian Cell Lineapplication demo
Inferred from claim c3 during normalization. The iLID-nano system was used to control talin-mediated cell spreading and migration. Derived from claim c3. Quoted text: We demonstrate the use of this system to control talin-mediated cell spreading and migration.
Source:
successMammalian Cell Lineapplication demo
Inferred from claim c4 during normalization. This work establishes a physical basis for using iLID-nano to directly control intramolecular force transmission in cells during mechanotransduction. Derived from claim c4. Quoted text: Together, we establish the physical basis for utilizing the iLID-nano system in the direct control of intramolecular force transmission in cells during mechanotransduction processes.
Source:
Supporting Sources
Ranked Claims
The iLID-nano system was used to control talin-mediated cell spreading and migration.
We demonstrate the use of this system to control talin-mediated cell spreading and migration.
The iLID-nano system was used to control talin-mediated cell spreading and migration.
We demonstrate the use of this system to control talin-mediated cell spreading and migration.
The iLID-nano system was used to control talin-mediated cell spreading and migration.
We demonstrate the use of this system to control talin-mediated cell spreading and migration.
The iLID-nano system was used to control talin-mediated cell spreading and migration.
We demonstrate the use of this system to control talin-mediated cell spreading and migration.
The iLID-nano system was used to control talin-mediated cell spreading and migration.
We demonstrate the use of this system to control talin-mediated cell spreading and migration.
The iLID-nano system was used to control talin-mediated cell spreading and migration.
We demonstrate the use of this system to control talin-mediated cell spreading and migration.
The iLID-nano system was used to control talin-mediated cell spreading and migration.
We demonstrate the use of this system to control talin-mediated cell spreading and migration.
The mechanical stability of iLID-nano suggests it can be used to modulate mechanotransduction processes involving similar force ranges.
The mechanical stability of the system suggests that it can be employed to modulate mechanotransduction processes that involve similar force ranges.
The mechanical stability of iLID-nano suggests it can be used to modulate mechanotransduction processes involving similar force ranges.
The mechanical stability of the system suggests that it can be employed to modulate mechanotransduction processes that involve similar force ranges.
The mechanical stability of iLID-nano suggests it can be used to modulate mechanotransduction processes involving similar force ranges.
The mechanical stability of the system suggests that it can be employed to modulate mechanotransduction processes that involve similar force ranges.
The mechanical stability of iLID-nano suggests it can be used to modulate mechanotransduction processes involving similar force ranges.
The mechanical stability of the system suggests that it can be employed to modulate mechanotransduction processes that involve similar force ranges.
The mechanical stability of iLID-nano suggests it can be used to modulate mechanotransduction processes involving similar force ranges.
The mechanical stability of the system suggests that it can be employed to modulate mechanotransduction processes that involve similar force ranges.
The mechanical stability of iLID-nano suggests it can be used to modulate mechanotransduction processes involving similar force ranges.
The mechanical stability of the system suggests that it can be employed to modulate mechanotransduction processes that involve similar force ranges.
The mechanical stability of iLID-nano suggests it can be used to modulate mechanotransduction processes involving similar force ranges.
The mechanical stability of the system suggests that it can be employed to modulate mechanotransduction processes that involve similar force ranges.
The iLID-nano optogenetic switch can withstand forces up to 10 pN for seconds to tens of seconds, with stability decreasing as force increases.
Our results show that the iLID-nano system can withstand forces up to 10 pN for seconds to tens of seconds that decrease as the force increases.
withstand duration seconds to tens of secondswithstood force 10 pN
The iLID-nano optogenetic switch can withstand forces up to 10 pN for seconds to tens of seconds, with stability decreasing as force increases.
Our results show that the iLID-nano system can withstand forces up to 10 pN for seconds to tens of seconds that decrease as the force increases.
withstand duration seconds to tens of secondswithstood force 10 pN
The iLID-nano optogenetic switch can withstand forces up to 10 pN for seconds to tens of seconds, with stability decreasing as force increases.
Our results show that the iLID-nano system can withstand forces up to 10 pN for seconds to tens of seconds that decrease as the force increases.
withstand duration seconds to tens of secondswithstood force 10 pN
The iLID-nano optogenetic switch can withstand forces up to 10 pN for seconds to tens of seconds, with stability decreasing as force increases.
Our results show that the iLID-nano system can withstand forces up to 10 pN for seconds to tens of seconds that decrease as the force increases.
withstand duration seconds to tens of secondswithstood force 10 pN
The iLID-nano optogenetic switch can withstand forces up to 10 pN for seconds to tens of seconds, with stability decreasing as force increases.
Our results show that the iLID-nano system can withstand forces up to 10 pN for seconds to tens of seconds that decrease as the force increases.
withstand duration seconds to tens of secondswithstood force 10 pN
The iLID-nano optogenetic switch can withstand forces up to 10 pN for seconds to tens of seconds, with stability decreasing as force increases.
Our results show that the iLID-nano system can withstand forces up to 10 pN for seconds to tens of seconds that decrease as the force increases.
withstand duration seconds to tens of secondswithstood force 10 pN
The iLID-nano optogenetic switch can withstand forces up to 10 pN for seconds to tens of seconds, with stability decreasing as force increases.
Our results show that the iLID-nano system can withstand forces up to 10 pN for seconds to tens of seconds that decrease as the force increases.
withstand duration seconds to tens of secondswithstood force 10 pN
This work establishes a physical basis for using iLID-nano to directly control intramolecular force transmission in cells during mechanotransduction.
Together, we establish the physical basis for utilizing the iLID-nano system in the direct control of intramolecular force transmission in cells during mechanotransduction processes.
This work establishes a physical basis for using iLID-nano to directly control intramolecular force transmission in cells during mechanotransduction.
Together, we establish the physical basis for utilizing the iLID-nano system in the direct control of intramolecular force transmission in cells during mechanotransduction processes.
This work establishes a physical basis for using iLID-nano to directly control intramolecular force transmission in cells during mechanotransduction.
Together, we establish the physical basis for utilizing the iLID-nano system in the direct control of intramolecular force transmission in cells during mechanotransduction processes.
This work establishes a physical basis for using iLID-nano to directly control intramolecular force transmission in cells during mechanotransduction.
Together, we establish the physical basis for utilizing the iLID-nano system in the direct control of intramolecular force transmission in cells during mechanotransduction processes.
This work establishes a physical basis for using iLID-nano to directly control intramolecular force transmission in cells during mechanotransduction.
Together, we establish the physical basis for utilizing the iLID-nano system in the direct control of intramolecular force transmission in cells during mechanotransduction processes.
This work establishes a physical basis for using iLID-nano to directly control intramolecular force transmission in cells during mechanotransduction.
Together, we establish the physical basis for utilizing the iLID-nano system in the direct control of intramolecular force transmission in cells during mechanotransduction processes.
This work establishes a physical basis for using iLID-nano to directly control intramolecular force transmission in cells during mechanotransduction.
Together, we establish the physical basis for utilizing the iLID-nano system in the direct control of intramolecular force transmission in cells during mechanotransduction processes.
Approval Evidence
1 source4 linked approval claimsfirst-pass slug ilid-nano
we quantify a frequently used molecular optogenetic switch, iLID-nano, which is an improved light-induced dimerization between LOV2-SsrA and SspB
Source:
application demosupports
The iLID-nano system was used to control talin-mediated cell spreading and migration.
We demonstrate the use of this system to control talin-mediated cell spreading and migration.
Source:
application relevancesupports
The mechanical stability of iLID-nano suggests it can be used to modulate mechanotransduction processes involving similar force ranges.
The mechanical stability of the system suggests that it can be employed to modulate mechanotransduction processes that involve similar force ranges.
Source:
mechanical stabilitysupports
The iLID-nano optogenetic switch can withstand forces up to 10 pN for seconds to tens of seconds, with stability decreasing as force increases.
Our results show that the iLID-nano system can withstand forces up to 10 pN for seconds to tens of seconds that decrease as the force increases.
Source:
physical basissupports
This work establishes a physical basis for using iLID-nano to directly control intramolecular force transmission in cells during mechanotransduction.
Together, we establish the physical basis for utilizing the iLID-nano system in the direct control of intramolecular force transmission in cells during mechanotransduction processes.
Source:
Comparisons
Source-backed strengths
The system is described as an improved light-induced dimerization between LOV2-SsrA and SspB, indicating an optimized heterodimerization module. It was applied to control talin-mediated cell spreading and migration, and its mechanical stability was quantified to withstand forces up to 10 pN for seconds to tens of seconds, although stability decreases as force increases.
Ranked Citations
- 1.