Source 1review2019Annual Review of Neuroscience
Toolkit/imaging
imaging
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
this view has been challenged in the last few years with the availability of RNA sequencing, immunohistochemistry, electron microscopy, morphological reconstruction, and imaging data
Usefulness & Problems
Why this is useful
Imaging is named as a general data source that helped overturn the assumption of astrocyte homogeneity. In this review, it functions as a broad observational modality.; observing astrocyte properties and heterogeneity
Source:
Imaging is named as a general data source that helped overturn the assumption of astrocyte homogeneity. In this review, it functions as a broad observational modality.
Source:
observing astrocyte properties and heterogeneity
Problem solved
It helps visualize astrocyte differences across brain areas or conditions.; provides observational evidence that astrocytes differ across contexts
Source:
It helps visualize astrocyte differences across brain areas or conditions.
Source:
provides observational evidence that astrocytes differ across contexts
Problem links
provides observational evidence that astrocytes differ across contexts
LiteratureIt helps visualize astrocyte differences across brain areas or conditions.
Source:
It helps visualize astrocyte differences across brain areas or conditions.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Mechanisms
observation-based visualizationTarget processes
No target processes tagged yet.
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor
The abstract only supports that imaging data were available. It does not identify microscopy type, reporters, or analysis methods.; requires imaging-compatible preparations and analysis
The abstract does not indicate that imaging alone defines molecular subtypes or causal mechanisms.; the abstract does not specify imaging modality or readout
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Recent availability of RNA sequencing, immunohistochemistry, electron microscopy, morphological reconstruction, and imaging data has challenged the view that astrocytes are a homogeneous population across the CNS.
Approval Evidence
1 source1 linked approval claimfirst-pass slug imaging
this view has been challenged in the last few years with the availability of RNA sequencing, immunohistochemistry, electron microscopy, morphological reconstruction, and imaging data
Source:
field summarysupports
Recent availability of RNA sequencing, immunohistochemistry, electron microscopy, morphological reconstruction, and imaging data has challenged the view that astrocytes are a homogeneous population across the CNS.
Source:
Comparisons
Source-stated alternatives
Other methods named in the abstract are RNA sequencing, immunohistochemistry, electron microscopy, and morphological reconstruction.
Source:
Other methods named in the abstract are RNA sequencing, immunohistochemistry, electron microscopy, and morphological reconstruction.
Source-backed strengths
included in the abstract as a major evidence stream supporting astrocyte diversity
Source:
included in the abstract as a major evidence stream supporting astrocyte diversity
Compared with electron microscopy
Other methods named in the abstract are RNA sequencing, immunohistochemistry, electron microscopy, and morphological reconstruction.
Shared frame: source-stated alternative in extracted literature
Strengths here: included in the abstract as a major evidence stream supporting astrocyte diversity.
Relative tradeoffs: the abstract does not specify imaging modality or readout.
Source:
Other methods named in the abstract are RNA sequencing, immunohistochemistry, electron microscopy, and morphological reconstruction.
Compared with immunohistochemistry
Other methods named in the abstract are RNA sequencing, immunohistochemistry, electron microscopy, and morphological reconstruction.
Shared frame: source-stated alternative in extracted literature
Strengths here: included in the abstract as a major evidence stream supporting astrocyte diversity.
Relative tradeoffs: the abstract does not specify imaging modality or readout.
Source:
Other methods named in the abstract are RNA sequencing, immunohistochemistry, electron microscopy, and morphological reconstruction.
Compared with microscopy
Other methods named in the abstract are RNA sequencing, immunohistochemistry, electron microscopy, and morphological reconstruction.
Shared frame: source-stated alternative in extracted literature
Strengths here: included in the abstract as a major evidence stream supporting astrocyte diversity.
Relative tradeoffs: the abstract does not specify imaging modality or readout.
Source:
Other methods named in the abstract are RNA sequencing, immunohistochemistry, electron microscopy, and morphological reconstruction.
Compared with morphological reconstruction
Other methods named in the abstract are RNA sequencing, immunohistochemistry, electron microscopy, and morphological reconstruction.
Shared frame: source-stated alternative in extracted literature
Strengths here: included in the abstract as a major evidence stream supporting astrocyte diversity.
Relative tradeoffs: the abstract does not specify imaging modality or readout.
Source:
Other methods named in the abstract are RNA sequencing, immunohistochemistry, electron microscopy, and morphological reconstruction.
Compared with RNA sequencing
Other methods named in the abstract are RNA sequencing, immunohistochemistry, electron microscopy, and morphological reconstruction.
Shared frame: source-stated alternative in extracted literature
Strengths here: included in the abstract as a major evidence stream supporting astrocyte diversity.
Relative tradeoffs: the abstract does not specify imaging modality or readout.
Source:
Other methods named in the abstract are RNA sequencing, immunohistochemistry, electron microscopy, and morphological reconstruction.
Ranked Citations
- 1.