Source 1primary paper2016Applied and Environmental Microbiology
Toolkit/lac promoter-based gene expression
lac promoter-based gene expression
Also known as: lac-based gene expression
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
This tool is a light-mediated implementation of lac promoter-based gene expression that uses photocaged isopropyl β-D-thiogalactopyranoside (IPTG) as a synthetic inducer. In Corynebacterium glutamicum, it was applied for light-mediated optimization of lac promoter-driven expression and (+)-valencene biosynthesis.
Usefulness & Problems
Why this is useful
The reported utility is optical control of lac-based expression for synthetic biotechnology. The source states that light induction can provide precise, homogeneous, noninvasive, and spatiotemporal control, particularly for increasing numbers of parallelized expression cultures.
Source:
Especially for increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction qualifies for a precise, homogeneous, and thus higher-order control to fully automatize or optimize future biotechnological applications.
Source:
Specifically, light-controlled gene expression exhibits an enormous potential for various synthetic bio(techno)logical purposes. Especially for increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction qualifies for a precise, homogeneous, and thus higher-order control to fully automatize or optimize future biotechnological applications.
Problem solved
This approach addresses the need to control lac promoter-based gene expression with a noninvasive external input rather than conventional bulk chemical induction alone. It is presented as a way to support parallelized culture control and optimization in biotechnological applications, including pathway optimization for (+)-valencene production in C. glutamicum.
Source:
Especially for increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction qualifies for a precise, homogeneous, and thus higher-order control to fully automatize or optimize future biotechnological applications.
Source:
Specifically, light-controlled gene expression exhibits an enormous potential for various synthetic bio(techno)logical purposes. Especially for increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction qualifies for a precise, homogeneous, and thus higher-order control to fully automatize or optimize future biotechnological applications.
Problem links
Need precise spatiotemporal control with light input
DerivedThis tool is a light-mediated implementation of lac promoter-based gene expression that uses photocaged isopropyl β-D-thiogalactopyranoside (IPTG) as a synthetic inducer. In Corynebacterium glutamicum, it was applied for light-mediated optimization of lac promoter-driven expression and (+)-valencene biosynthesis.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Techniques
No technique tags yet.
Target processes
No target processes tagged yet.
Input: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: actuatorswitch architecture: cleavage
The implementation described in the source uses photocaged IPTG to mediate light-responsive induction of a lac promoter-based expression system. The evidence specifically supports use in Corynebacterium glutamicum, but the provided material does not detail construct architecture, illumination parameters, or delivery conditions for the caged inducer.
The supplied evidence is limited to a single 2016 source and provides little quantitative performance information such as induction fold, kinetics, leakiness, light dose requirements, or wavelength. Independent replication and validation outside Corynebacterium glutamicum are not documented in the provided evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
For increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction provides precise, homogeneous, higher-order control that could help automate or optimize future biotechnological applications.
Especially for increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction qualifies for a precise, homogeneous, and thus higher-order control to fully automatize or optimize future biotechnological applications.
For increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction provides precise, homogeneous, higher-order control that could help automate or optimize future biotechnological applications.
Especially for increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction qualifies for a precise, homogeneous, and thus higher-order control to fully automatize or optimize future biotechnological applications.
For increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction provides precise, homogeneous, higher-order control that could help automate or optimize future biotechnological applications.
Especially for increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction qualifies for a precise, homogeneous, and thus higher-order control to fully automatize or optimize future biotechnological applications.
For increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction provides precise, homogeneous, higher-order control that could help automate or optimize future biotechnological applications.
Especially for increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction qualifies for a precise, homogeneous, and thus higher-order control to fully automatize or optimize future biotechnological applications.
For increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction provides precise, homogeneous, higher-order control that could help automate or optimize future biotechnological applications.
Especially for increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction qualifies for a precise, homogeneous, and thus higher-order control to fully automatize or optimize future biotechnological applications.
For increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction provides precise, homogeneous, higher-order control that could help automate or optimize future biotechnological applications.
Especially for increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction qualifies for a precise, homogeneous, and thus higher-order control to fully automatize or optimize future biotechnological applications.
For increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction provides precise, homogeneous, higher-order control that could help automate or optimize future biotechnological applications.
Especially for increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction qualifies for a precise, homogeneous, and thus higher-order control to fully automatize or optimize future biotechnological applications.
Light-controlled gene expression has strong potential for synthetic biotechnological applications and can provide precise, homogeneous, noninvasive, and spatiotemporal control for parallelized expression cultures.
Specifically, light-controlled gene expression exhibits an enormous potential for various synthetic bio(techno)logical purposes. Especially for increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction qualifies for a precise, homogeneous, and thus higher-order control to fully automatize or optimize future biotechnological applications.
Light-controlled gene expression has strong potential for synthetic biotechnological applications and can provide precise, homogeneous, noninvasive, and spatiotemporal control for parallelized expression cultures.
Specifically, light-controlled gene expression exhibits an enormous potential for various synthetic bio(techno)logical purposes. Especially for increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction qualifies for a precise, homogeneous, and thus higher-order control to fully automatize or optimize future biotechnological applications.
Light-controlled gene expression has strong potential for synthetic biotechnological applications and can provide precise, homogeneous, noninvasive, and spatiotemporal control for parallelized expression cultures.
Specifically, light-controlled gene expression exhibits an enormous potential for various synthetic bio(techno)logical purposes. Especially for increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction qualifies for a precise, homogeneous, and thus higher-order control to fully automatize or optimize future biotechnological applications.
Light-controlled gene expression has strong potential for synthetic biotechnological applications and can provide precise, homogeneous, noninvasive, and spatiotemporal control for parallelized expression cultures.
Specifically, light-controlled gene expression exhibits an enormous potential for various synthetic bio(techno)logical purposes. Especially for increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction qualifies for a precise, homogeneous, and thus higher-order control to fully automatize or optimize future biotechnological applications.
Light-controlled gene expression has strong potential for synthetic biotechnological applications and can provide precise, homogeneous, noninvasive, and spatiotemporal control for parallelized expression cultures.
Specifically, light-controlled gene expression exhibits an enormous potential for various synthetic bio(techno)logical purposes. Especially for increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction qualifies for a precise, homogeneous, and thus higher-order control to fully automatize or optimize future biotechnological applications.
Light-controlled gene expression has strong potential for synthetic biotechnological applications and can provide precise, homogeneous, noninvasive, and spatiotemporal control for parallelized expression cultures.
Specifically, light-controlled gene expression exhibits an enormous potential for various synthetic bio(techno)logical purposes. Especially for increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction qualifies for a precise, homogeneous, and thus higher-order control to fully automatize or optimize future biotechnological applications.
Light-controlled gene expression has strong potential for synthetic biotechnological applications and can provide precise, homogeneous, noninvasive, and spatiotemporal control for parallelized expression cultures.
Specifically, light-controlled gene expression exhibits an enormous potential for various synthetic bio(techno)logical purposes. Especially for increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction qualifies for a precise, homogeneous, and thus higher-order control to fully automatize or optimize future biotechnological applications.
Light-controlled gene expression has strong potential for synthetic biotechnological applications and can provide precise, homogeneous, noninvasive, and spatiotemporal control for parallelized expression cultures.
Specifically, light-controlled gene expression exhibits an enormous potential for various synthetic bio(techno)logical purposes. Especially for increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction qualifies for a precise, homogeneous, and thus higher-order control to fully automatize or optimize future biotechnological applications.
Light-controlled gene expression has strong potential for synthetic biotechnological applications and can provide precise, homogeneous, noninvasive, and spatiotemporal control for parallelized expression cultures.
Specifically, light-controlled gene expression exhibits an enormous potential for various synthetic bio(techno)logical purposes. Especially for increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction qualifies for a precise, homogeneous, and thus higher-order control to fully automatize or optimize future biotechnological applications.
The study concerns light-mediated optimization of (+)-valencene biosynthesis in Corynebacterium glutamicum.
Light-Controlled Cell Factories: Employing Photocaged Isopropyl-b2- <scp>d</scp> -Thiogalactopyranoside for Light-Mediated Optimization of lac Promoter-Based Gene Expression and (+)-Valencene Biosynthesis in Corynebacterium glutamicum
The study concerns light-mediated optimization of (+)-valencene biosynthesis in Corynebacterium glutamicum.
Light-Controlled Cell Factories: Employing Photocaged Isopropyl-b2- <scp>d</scp> -Thiogalactopyranoside for Light-Mediated Optimization of lac Promoter-Based Gene Expression and (+)-Valencene Biosynthesis in Corynebacterium glutamicum
The study concerns light-mediated optimization of (+)-valencene biosynthesis in Corynebacterium glutamicum.
Light-Controlled Cell Factories: Employing Photocaged Isopropyl-b2- <scp>d</scp> -Thiogalactopyranoside for Light-Mediated Optimization of lac Promoter-Based Gene Expression and (+)-Valencene Biosynthesis in Corynebacterium glutamicum
The study concerns light-mediated optimization of (+)-valencene biosynthesis in Corynebacterium glutamicum.
Light-Controlled Cell Factories: Employing Photocaged Isopropyl-b2- <scp>d</scp> -Thiogalactopyranoside for Light-Mediated Optimization of lac Promoter-Based Gene Expression and (+)-Valencene Biosynthesis in Corynebacterium glutamicum
The study concerns light-mediated optimization of (+)-valencene biosynthesis in Corynebacterium glutamicum.
Light-Controlled Cell Factories: Employing Photocaged Isopropyl-b2- <scp>d</scp> -Thiogalactopyranoside for Light-Mediated Optimization of lac Promoter-Based Gene Expression and (+)-Valencene Biosynthesis in Corynebacterium glutamicum
The study concerns light-mediated optimization of (+)-valencene biosynthesis in Corynebacterium glutamicum.
Light-Controlled Cell Factories: Employing Photocaged Isopropyl-b2- <scp>d</scp> -Thiogalactopyranoside for Light-Mediated Optimization of lac Promoter-Based Gene Expression and (+)-Valencene Biosynthesis in Corynebacterium glutamicum
The study concerns light-mediated optimization of (+)-valencene biosynthesis in Corynebacterium glutamicum.
Light-Controlled Cell Factories: Employing Photocaged Isopropyl-b2- <scp>d</scp> -Thiogalactopyranoside for Light-Mediated Optimization of lac Promoter-Based Gene Expression and (+)-Valencene Biosynthesis in Corynebacterium glutamicum
In Corynebacterium glutamicum, applying photocaged IPTG as a synthetic inducer could almost completely abolish poor inducibility and phenotypic heterogeneity associated with IPTG-mediated induction of lac-based gene expression.
Before our study, poor inducibility, together with phenotypic heterogeneity, was reported for the IPTG-mediated induction of lac-based gene expression in Corynebacterium glutamicum By applying photocaged IPTG as a synthetic inducer, however, these drawbacks could be almost completely abolished.
In Corynebacterium glutamicum, applying photocaged IPTG as a synthetic inducer could almost completely abolish poor inducibility and phenotypic heterogeneity associated with IPTG-mediated induction of lac-based gene expression.
Before our study, poor inducibility, together with phenotypic heterogeneity, was reported for the IPTG-mediated induction of lac-based gene expression in Corynebacterium glutamicum By applying photocaged IPTG as a synthetic inducer, however, these drawbacks could be almost completely abolished.
In Corynebacterium glutamicum, applying photocaged IPTG as a synthetic inducer could almost completely abolish poor inducibility and phenotypic heterogeneity associated with IPTG-mediated induction of lac-based gene expression.
Before our study, poor inducibility, together with phenotypic heterogeneity, was reported for the IPTG-mediated induction of lac-based gene expression in Corynebacterium glutamicum By applying photocaged IPTG as a synthetic inducer, however, these drawbacks could be almost completely abolished.
In Corynebacterium glutamicum, applying photocaged IPTG as a synthetic inducer could almost completely abolish poor inducibility and phenotypic heterogeneity associated with IPTG-mediated induction of lac-based gene expression.
Before our study, poor inducibility, together with phenotypic heterogeneity, was reported for the IPTG-mediated induction of lac-based gene expression in Corynebacterium glutamicum By applying photocaged IPTG as a synthetic inducer, however, these drawbacks could be almost completely abolished.
In Corynebacterium glutamicum, applying photocaged IPTG as a synthetic inducer could almost completely abolish poor inducibility and phenotypic heterogeneity associated with IPTG-mediated induction of lac-based gene expression.
Before our study, poor inducibility, together with phenotypic heterogeneity, was reported for the IPTG-mediated induction of lac-based gene expression in Corynebacterium glutamicum By applying photocaged IPTG as a synthetic inducer, however, these drawbacks could be almost completely abolished.
In Corynebacterium glutamicum, applying photocaged IPTG as a synthetic inducer could almost completely abolish poor inducibility and phenotypic heterogeneity associated with IPTG-mediated induction of lac-based gene expression.
Before our study, poor inducibility, together with phenotypic heterogeneity, was reported for the IPTG-mediated induction of lac-based gene expression in Corynebacterium glutamicum By applying photocaged IPTG as a synthetic inducer, however, these drawbacks could be almost completely abolished.
In Corynebacterium glutamicum, applying photocaged IPTG as a synthetic inducer could almost completely abolish poor inducibility and phenotypic heterogeneity associated with IPTG-mediated induction of lac-based gene expression.
Before our study, poor inducibility, together with phenotypic heterogeneity, was reported for the IPTG-mediated induction of lac-based gene expression in Corynebacterium glutamicum By applying photocaged IPTG as a synthetic inducer, however, these drawbacks could be almost completely abolished.
In Corynebacterium glutamicum, applying photocaged IPTG as a synthetic inducer could almost completely abolish poor inducibility and phenotypic heterogeneity associated with IPTG-mediated induction of lac-based gene expression.
Before our study, poor inducibility, together with phenotypic heterogeneity, was reported for the IPTG-mediated induction of lac-based gene expression in Corynebacterium glutamicum By applying photocaged IPTG as a synthetic inducer, however, these drawbacks could be almost completely abolished.
In Corynebacterium glutamicum, applying photocaged IPTG as a synthetic inducer could almost completely abolish poor inducibility and phenotypic heterogeneity associated with IPTG-mediated induction of lac-based gene expression.
Before our study, poor inducibility, together with phenotypic heterogeneity, was reported for the IPTG-mediated induction of lac-based gene expression in Corynebacterium glutamicum By applying photocaged IPTG as a synthetic inducer, however, these drawbacks could be almost completely abolished.
In Corynebacterium glutamicum, applying photocaged IPTG as a synthetic inducer could almost completely abolish poor inducibility and phenotypic heterogeneity associated with IPTG-mediated induction of lac-based gene expression.
Before our study, poor inducibility, together with phenotypic heterogeneity, was reported for the IPTG-mediated induction of lac-based gene expression in Corynebacterium glutamicum By applying photocaged IPTG as a synthetic inducer, however, these drawbacks could be almost completely abolished.
In Corynebacterium glutamicum, applying photocaged IPTG as a synthetic inducer could almost completely abolish poor inducibility and phenotypic heterogeneity associated with IPTG-mediated induction of lac-based gene expression.
Before our study, poor inducibility, together with phenotypic heterogeneity, was reported for the IPTG-mediated induction of lac-based gene expression in Corynebacterium glutamicum By applying photocaged IPTG as a synthetic inducer, however, these drawbacks could be almost completely abolished.
In Corynebacterium glutamicum, applying photocaged IPTG as a synthetic inducer could almost completely abolish poor inducibility and phenotypic heterogeneity associated with IPTG-mediated induction of lac-based gene expression.
Before our study, poor inducibility, together with phenotypic heterogeneity, was reported for the IPTG-mediated induction of lac-based gene expression in Corynebacterium glutamicum By applying photocaged IPTG as a synthetic inducer, however, these drawbacks could be almost completely abolished.
In Corynebacterium glutamicum, applying photocaged IPTG as a synthetic inducer could almost completely abolish poor inducibility and phenotypic heterogeneity associated with IPTG-mediated induction of lac-based gene expression.
Before our study, poor inducibility, together with phenotypic heterogeneity, was reported for the IPTG-mediated induction of lac-based gene expression in Corynebacterium glutamicum By applying photocaged IPTG as a synthetic inducer, however, these drawbacks could be almost completely abolished.
In Corynebacterium glutamicum, applying photocaged IPTG as a synthetic inducer could almost completely abolish poor inducibility and phenotypic heterogeneity associated with IPTG-mediated induction of lac-based gene expression.
Before our study, poor inducibility, together with phenotypic heterogeneity, was reported for the IPTG-mediated induction of lac-based gene expression in Corynebacterium glutamicum By applying photocaged IPTG as a synthetic inducer, however, these drawbacks could be almost completely abolished.
In Corynebacterium glutamicum, applying photocaged IPTG as a synthetic inducer could almost completely abolish poor inducibility and phenotypic heterogeneity associated with IPTG-mediated induction of lac-based gene expression.
Before our study, poor inducibility, together with phenotypic heterogeneity, was reported for the IPTG-mediated induction of lac-based gene expression in Corynebacterium glutamicum By applying photocaged IPTG as a synthetic inducer, however, these drawbacks could be almost completely abolished.
In Corynebacterium glutamicum, applying photocaged IPTG as a synthetic inducer could almost completely abolish poor inducibility and phenotypic heterogeneity associated with IPTG-mediated induction of lac-based gene expression.
Before our study, poor inducibility, together with phenotypic heterogeneity, was reported for the IPTG-mediated induction of lac-based gene expression in Corynebacterium glutamicum By applying photocaged IPTG as a synthetic inducer, however, these drawbacks could be almost completely abolished.
Approval Evidence
1 source1 linked approval claimfirst-pass slug lac-promoter-based-gene-expression
light-mediated optimization of lac promoter-based gene expression
Source:
performance improvementsupports
In Corynebacterium glutamicum, applying photocaged IPTG as a synthetic inducer could almost completely abolish poor inducibility and phenotypic heterogeneity associated with IPTG-mediated induction of lac-based gene expression.
Before our study, poor inducibility, together with phenotypic heterogeneity, was reported for the IPTG-mediated induction of lac-based gene expression in Corynebacterium glutamicum By applying photocaged IPTG as a synthetic inducer, however, these drawbacks could be almost completely abolished.
Source:
Comparisons
Source-backed strengths
The cited study attributes to this system precise, homogeneous, noninvasive, and spatiotemporal control of gene expression under light induction. It was also demonstrated in an application context for optimization of (+)-valencene biosynthesis in Corynebacterium glutamicum.
Source:
Before our study, poor inducibility, together with phenotypic heterogeneity, was reported for the IPTG-mediated induction of lac-based gene expression in Corynebacterium glutamicum By applying photocaged IPTG as a synthetic inducer, however, these drawbacks could be almost completely abolished.
Compared with alkynyl-functionalized photocleavable linker
lac promoter-based gene expression and alkynyl-functionalized photocleavable linker address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: photocleavage; same primary input modality: light
Compared with Opto-Casp8-V1
lac promoter-based gene expression and Opto-Casp8-V1 address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: photocleavage; same primary input modality: light
Compared with Opto-Casp8-V2
lac promoter-based gene expression and Opto-Casp8-V2 address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: photocleavage; same primary input modality: light
Ranked Citations
- 1.